Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 781,668 C→T 7.2% intergenic (+16/‑417) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913781,6680CT 7.2% 495.9 / 20.0 167intergenic (+16/‑417)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
Reads supporting (aligned to +/- strand):  ref base C (72/82);  new base T (4/8);  total (76/91)
Fisher's exact test for biased strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

GAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAA  >  NC_000913/781521‑781816
                                                                                                                                                   |                                                                                                                                                    
 acctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaa                                                                                                                                                   >  1:2406897‑M1/56‑150 (MQ=255)
 aGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaa                                                                                                                                                   >  1:1917987/1‑150 (MQ=255)
 aGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaa                                                                                                                                                   >  1:2310194/1‑150 (MQ=255)
  gTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCTGTTGGTAGAGCAGTTGACTTTTATTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa                                                                                                                                                  <  1:654580/150‑1 (MQ=255)
  cctcccttacatggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTTGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa                                                                                                                                                  >  1:4032287‑M1/55‑150 (MQ=255)
  cctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa                                                                                                                                                  <  1:2727626‑M1/96‑1 (MQ=255)
  cctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa                                                                                                                                                  <  1:1981218‑M1/96‑1 (MQ=255)
   tCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGCGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                                                                                                                 >  1:387217/1‑150 (MQ=255)
   tCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                                                                                                                 <  2:1425429/150‑1 (MQ=255)
   ctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                                                                                                                 <  1:1796644‑M1/97‑1 (MQ=255)
    tcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg                                                                                                                                                <  1:776908‑M1/98‑1 (MQ=255)
    tcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAgg                                                                                                                                                <  2:3088676‑M1/98‑1 (MQ=255)
    cATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg                                                                                                                                                <  2:2798097/150‑1 (MQ=255)
    cATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg                                                                                                                                                >  1:3046438/1‑150 (MQ=255)
     aTCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATGAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc                                                                                                                                               <  2:89305/150‑1 (MQ=255)
     aTCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc                                                                                                                                               <  2:2112925/150‑1 (MQ=255)
     aTCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc                                                                                                                                               <  2:75867/150‑1 (MQ=255)
      tCCTGCACGACCCTCCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg                                                                                                                                              <  2:1450016/150‑1 (MQ=255)
      ccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg                                                                                                                                              <  2:1514703‑M1/100‑1 (MQ=255)
        ttacaaggagggggtcggcgggtcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGGTGGTAGAGCAGGTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCCATGTAAAAAAGCGCCCTAAAGGCGCt                                                                                                                                            <  2:1792200‑M1/102‑1 (MQ=255)
         tGCACGACCCACCATCCTTAATTATTAAGGCAGCATAATCCCGCACGGGGTCGTTAGCTCTGTTGGTTGAGCAGTTGACTTTTAATCAATGGGTCGCCGTTTCGAATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCtt                                                                                                                                           <  1:739452/150‑1 (MQ=18)
           cACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt                                                                                                                                         >  2:3937524/1‑150 (MQ=255)
           cACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt                                                                                                                                         >  2:929692/1‑150 (MQ=255)
           cACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCtttt                                                                                                                                         <  2:1991168/150‑1 (MQ=255)
            ccggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCACAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTACCGGCGCttttt                                                                                                                                        >  2:3216561‑M1/45‑150 (MQ=255)
            aaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttttt                                                                                                                                        >  1:303543‑M1/45‑150 (MQ=255)
            aCGACCCACCATCCTGACTGATTAAGGCAGCATACTCCCGCACGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAATTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCttttt                                                                                                                                        <  1:541645/150‑1 (MQ=18)
             cGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCCAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTCACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttttt                                                                                                                                       <  1:3516001/150‑1 (MQ=255)
             cGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttttt                                                                                                                                       <  1:2187832/150‑1 (MQ=255)
             cGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttttt                                                                                                                                       <  1:2187805/150‑1 (MQ=255)
                  caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCtct                                                                                                                                  >  1:2006205/1‑148 (MQ=255)
                  caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTAt                                                                                                                                  >  1:183152/1‑150 (MQ=255)
                  caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTAt                                                                                                                                  >  1:2006144/1‑150 (MQ=255)
                  caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTAt                                                                                                                                  >  1:1789092/1‑150 (MQ=255)
                    ccttCCAGAACGATAACGGCTGCATAACCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCt                                                                                                                                <  2:3669213/147‑1 (MQ=14)
                    ccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCt                                                                                                                                <  2:2633758/150‑1 (MQ=255)
                    ccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCt                                                                                                                                <  2:2558932/150‑1 (MQ=255)
                       tCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCg                                                                                                                             <  1:2559932/150‑1 (MQ=255)
                       cggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGGTCAGTTGGTAGAGCAGTTGACTTTTAATGAATTTGTCGCAGGTTGGAATCCTGCACGACCCACCAATGTAAATAAGCGCCCTAAAGGGGGTTTTTTTATatcttcg                                                                                                                             <  1:1876438‑M1/117‑4 (MQ=255)
                         cTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGAt                                                                                                                           <  1:1913728/150‑1 (MQ=255)
                          tGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATa                                                                                                                          >  1:3084776/1‑150 (MQ=255)
                             aTGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGCTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTc                                                                                                                       <  2:1915716/150‑1 (MQ=255)
                               gATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaa                                                                                                                     >  1:383007/1‑150 (MQ=255)
                               gATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaa                                                                                                                     <  1:4104881/150‑1 (MQ=255)
                                aatAAGGCAGCATAAGCCCGCAAGGGGTCGTGAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCACTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaaa                                                                                                                    <  1:1243911/148‑1 (MQ=255)
                                 ttAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAg                                                                                                                   >  1:3849703/1‑150 (MQ=255)
                                 atcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCGAAg                                                                                                                   <  2:317388‑M1/127‑1 (MQ=255)
                                    aGGCAGCATAATCCCGCACGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGAtt                                                                                                                <  1:2458994/150‑1 (MQ=255)
                                    aGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGAtt                                                                                                                <  2:2143417/150‑1 (MQ=255)
                                      gcagcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCg                                                                                                              >  1:1267899/1‑150 (MQ=255)
                                        cgcaTTATCCCTATAGGGGTCTTTAGCTCAGTTGGTAGATCTTTTGACTTTTAATCTATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGaa                                                                                                            <  1:3595415/149‑1 (MQ=11)
                                          tcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAGGCGCTTTTTTTCTATATGCGATACTCAAAGATTCGAAcc                                                                                                          <  1:3900464‑M1/136‑1 (MQ=255)
                                          caTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAAcc                                                                                                          >  2:3968659/1‑150 (MQ=255)
                                             aaTCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATAGGCGATACTAAAAGATTCGAACCTgc                                                                                                       >  1:29160/1‑150 (MQ=255)
                                              ccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAAAGTAAAAAAGCGCCCGAAAGGCGCTTTTTTTCAATCTGCGATAATCAAAGATTCGAACCTgca                                                                                                      <  1:675491‑M1/140‑1 (MQ=255)
                                              aTCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTgca                                                                                                      <  2:1365161/150‑1 (MQ=40)
                                               caccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTATCTGCGATACTCAAATATTCGAACCTgcag                                                                                                     <  1:3388267‑M1/141‑1 (MQ=255)
                                                      aGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt                                                                                              <  1:3265995/150‑1 (MQ=33)
                                                      aGGGGTCGTTAGCTCAGTGGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt                                                                                              <  1:3346513/150‑1 (MQ=18)
                                                        gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGGCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttga                                                                                            <  1:1134429/150‑1 (MQ=17)
                                                           tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagtt                                                                                         <  2:3315571/150‑1 (MQ=32)
                                                           tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAAGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagtt                                                                                         <  2:1111456/150‑1 (MQ=17)
                                                           tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAAGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagtt                                                                                         <  1:716030/150‑1 (MQ=17)
                                                             gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCAGCGCTACAAAAATATTAGAAACTGCAGCAGGTttgatatca                                                                                       >  1:2664092/1‑145 (MQ=11)
                                                                   tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATACGAACCTGCAGCAGGTTTGAGTTGATCGCAg                                                                                 >  2:2336167/1‑150 (MQ=17)
                                                                       ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGaa                                                                             >  2:1558787/1‑150 (MQ=32)
                                                                            agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaacaac                                                                        <  2:1864027/150‑1 (MQ=32)
                                                                              agCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACgg                                                                      <  2:3026323/150‑1 (MQ=32)
                                                                                cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAg                                                                    >  1:3659653/1‑150 (MQ=32)
                                                                                   ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCg                                                                 >  2:537447/1‑150 (MQ=32)
                                                                                       cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcg                                                             >  1:3650805/1‑150 (MQ=32)
                                                                                        ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc                                                            >  2:1830447/1‑150 (MQ=32)
                                                                                        ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc                                                            >  1:1029101/1‑150 (MQ=32)
                                                                                              tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATATGCGAAACACAAATATACGAGCCAGCAGCAGCATTGAGATGAGCTCAGCGAAAACAAGGAGCCGCTCGCGGCGAc                                                      >  1:1079859/1‑150 (MQ=11)
                                                                                               cAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACg                                                     >  1:2808406/1‑150 (MQ=32)
                                                                                                 aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTCTCTGGGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGc                                                   >  1:657098/1‑150 (MQ=17)
                                                                                                      tCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGaa                                              >  1:1747206/1‑150 (MQ=32)
                                                                                                          aGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgc                                          >  1:2668591/1‑150 (MQ=32)
                                                                                                            gTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATGTGCGATACACAAATATACGAACATGCGGCAGGATTGAGTTGAGAGAACCGAAACAACTGAGCCGCTCGCGGCGACGGCCCGAAGGgcga                                        >  1:2802391/1‑150 (MQ=11)
                                                                                                             ttCGACTCCTGCTCGCCCCACCCCGGTCACCACGCGCCCTACAGGCGCTTTTTGGCTCTCTGCGATCCTCAACGATTCGACCCTGCAGCTGGTGTGAGTGGAGGGCCGCGACACTCCGGAGCCGCGCGCGGCGACGGCCCGAAGGgcgag                                       <  2:812116/150‑1 (MQ=2)
                                                                                                             ttCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgag                                       >  1:1030020/1‑150 (MQ=32)
                                                                                                                  aTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaag                                  >  1:1093013/1‑150 (MQ=32)
                                                                                                                   tCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                 >  2:2892466/1‑150 (MQ=32)
                                                                                                                   tCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                 <  2:2310194/150‑1 (MQ=32)
                                                                                                                   tCCGGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCCGGTTTGTGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                 <  2:1917987/150‑1 (MQ=255)
                                                                                                                   accTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                 >  1:3454439/2‑150 (MQ=17)
                                                                                                                     cTGCACGACCCTCCAATGTAAAAAAGCGCCCTTAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCTGTTTTGAGTTGTGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcga                               <  2:2074298/150‑1 (MQ=11)
                                                                                                                     cTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcga                               <  2:272325/150‑1 (MQ=32)
                                                                                                                      tGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAg                              <  2:3659653/150‑1 (MQ=32)
                                                                                                                         aCGACCCTCCACTGTCTTATAGCGCCGTATAGGCGCTTTTTTGCTATCTGCGATTCTCAAAGTTTCGAACATGCAGCAGGTTTGAGTTGAGCGCAGCGTAACTACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCa                           <  2:1699205/150‑1 (MQ=11)
                                                                                                                           caCCCACCAATGAAAAAAAGCCCCTTACTGGGGATTTTTTGCTATGTGCGATAATAAAATTTTCCAACCTGCTGCTGTTTTTTTTTGCGCGCAGCCAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATc                         <  1:4020618/149‑1 (MQ=2)
                                                                                                                             ccccccACTGTAAAAAAGCGCCCTACAGGCGCTTTTTTGCTATCTGCGATACTCAACGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCt                       <  2:3299328/150‑1 (MQ=255)
                                                                                                                              ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACACCAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTg                      >  2:2052895/1‑150 (MQ=17)
                                                                                                                               caccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATAGTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGc                     >  2:2476110/1‑150 (MQ=17)
                                                                                                                                accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                    >  2:3307784/1‑150 (MQ=32)
                                                                                                                                accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                    >  1:1627692/1‑150 (MQ=32)
                                                                                                                                accaATGTAAAAAAGCGCCCTAAAGGCGCTGTTTTGCTATCTGCGATACTCAAAGATTCGACCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                    >  2:2769708/1‑150 (MQ=17)
                                                                                                                                       aaaaaaaGCGCCCTAACGGCTCTTTTTTGCTTTCTGCCATACTCACAGACTCGAACCTGCAGCGGTTTTGCTTTGAGCGCAGCTAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACcca             <  1:3375469/149‑1 (MQ=11)
                                                                                                                                        aaaaaaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac            >  2:3792869/1‑150 (MQ=32)
                                                                                                                                         aaaaaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc           >  1:2163830/1‑150 (MQ=32)
                                                                                                                                                   |                                                                                                                                                    
GAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAA  >  NC_000913/781521‑781816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: