| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 781,672 | A→G | 7.0% | intergenic (+20/‑413) | lysQ → / → nadA | tRNA‑Lys/quinolinate synthase, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 781,672 | 0 | A | G | 7.0% | 443.4 / 19.7 | 159 | intergenic (+20/‑413) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
| Reads supporting (aligned to +/- strand): ref base A (65/77); new base G (4/7); total (70/89) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01 | |||||||||||
Only 100 of 167 total aligned reads displayed.
GTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCG > NC_000913/781523‑781821 | gTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCTGTTGGTAGAGCAGTTGACTTTTATTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa < 1:654580/150‑1 (MQ=255)cctcccttacttggtgggggtcggcggttcgatcccgtcatcacccaccacttgGGGTCGTTAGCTCTTTTGGTAGAGCTGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa > 1:4032224‑M1/55‑150 (MQ=255)cctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa < 1:2727626‑M1/96‑1 (MQ=255)cctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa < 1:1981218‑M1/96‑1 (MQ=255) tCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg < 2:1425429/150‑1 (MQ=255) ctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTCGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAg < 1:1796674‑M1/97‑1 (MQ=255) tcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg < 1:776908‑M1/98‑1 (MQ=255) tcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAgg < 2:3088676‑M1/98‑1 (MQ=255) cATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg < 2:2798097/150‑1 (MQ=255) cATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg > 1:3046438/1‑150 (MQ=255) aTCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATGAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc < 2:89305/150‑1 (MQ=255) aTCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc < 2:3181051/150‑1 (MQ=255) aTCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc < 2:75867/150‑1 (MQ=255) aTCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc < 2:2112925/150‑1 (MQ=255) tCCTGCACGACCCTCCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg < 2:1450016/150‑1 (MQ=255) ccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg < 2:1514703‑M1/100‑1 (MQ=255) ttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCt < 1:170972‑M1/102‑1 (MQ=255) ttacaaggagggggtcggcgggtcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGGTGGTAGAGCAGGTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCCATGTAAAAAAGCGCCCTAAAGGCGCt < 2:1792200‑M1/102‑1 (MQ=255) cTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCt > 1:1991168/1‑150 (MQ=255) tGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtt < 1:2185479/150‑1 (MQ=255) gCACCACCCACCAACCTGAATGATTAAGGCAGCATAACCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTAAATCATTTGGTCGCCGGTTCGAATCCTGCACGACCCACCAATTTAAAAAAGCGCCTTTAGGGCGCttt < 1:3902350/150‑1 (MQ=18) cACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt > 2:929692/1‑150 (MQ=255) cACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt > 2:3937524/1‑150 (MQ=255) cACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCtttt < 2:1991168/150‑1 (MQ=255) aaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttttt > 1:303543‑M1/45‑150 (MQ=255) cGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttttt < 1:2187832/150‑1 (MQ=255) gACCCACCATCCTGAATTTTTATGTCAGCATAATCCCGCAAGGGGTCGTTATCTCAGTTGGTAGCGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCtttttta < 1:3810821/150‑2 (MQ=17) caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCtct > 1:2006205/1‑148 (MQ=255) caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTAt > 1:1789092/1‑150 (MQ=255) caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCAAAAGGCGCTTTTTTGCTAt > 1:1789083/1‑150 (MQ=255) ccttCCAGAACGATAACGGCTGCATAACCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCt < 2:3669213/147‑1 (MQ=14) ccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCt < 2:2633758/150‑1 (MQ=255) tCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCg < 1:2559932/150‑1 (MQ=255) cggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGGTCAGTTGGTAGAGCAGTTGACTTTTAATGAATTTGTCGCAGGTTGGAATCCTGCACGACCCACCAATGTAAATAAGCGCCCTAAAGGGGGTTTTTTTATatcttcg < 1:1876438‑M1/117‑4 (MQ=255) cTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGAt < 1:234283/150‑1 (MQ=255) aTGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGCTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTc < 2:1915716/150‑1 (MQ=255) gATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaa < 1:4104881/150‑1 (MQ=255) gATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaa > 1:383007/1‑150 (MQ=255) aatAAGGCAGCATAAGCCCGCAAGGGGTCGTGAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCACTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaaa < 1:1243911/148‑1 (MQ=255) ttAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAg > 1:3849703/1‑150 (MQ=255) atcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCGAAg < 2:317388‑M1/127‑1 (MQ=255) aGGCAGCATAATCCCGCACGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGAtt < 1:2458994/150‑1 (MQ=255) aGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGAtt < 2:2143417/150‑1 (MQ=255) gcagcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCg > 1:1267899/1‑150 (MQ=255) cagcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGa < 1:1840589/150‑1 (MQ=255) cgcaTTATCCCTATAGGGGTCTTTAGCTCAGTTGGTAGATCTTTTGACTTTTAATCTATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGaa < 1:3595415/149‑1 (MQ=11) agcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGaa < 1:3595389/150‑1 (MQ=255) tcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAGGCGCTTTTTTTCTATATGCGATACTCAAAGATTCGAAcc < 1:3900464‑M1/136‑1 (MQ=255) caTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAAcc > 2:3968659/1‑150 (MQ=255) aTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCt > 1:2741739/1‑150 (MQ=255) ccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAAAGTAAAAAAGCGCCCGAAAGGCGCTTTTTTTCAATCTGCGATAATCAAAGATTCGAACCTgca < 1:675491‑M1/140‑1 (MQ=255) aTCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTgca < 2:1365161/150‑1 (MQ=40) caccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTATCTGCGATACTCAAATATTCGAACCTgcag < 1:3388267‑M1/141‑1 (MQ=255) caccacttcGGGTCGTTAGCGCAGTTGGTAGAGCAGTTGACTTTTAATCAATTTGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAATAGCCCAAAAGGCGCTTTTTTTATATATGCGAAAGTCAAAATTTCCAACCGgcag < 1:2994221‑M1/141‑1 (MQ=255) aGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt < 1:3265995/150‑1 (MQ=33) gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGGCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttga < 1:1134429/150‑1 (MQ=17) tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAAGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagtt < 1:716030/150‑1 (MQ=17) tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAAGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagtt < 1:1917615/150‑1 (MQ=17) tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAAGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagtt < 2:1111456/150‑1 (MQ=17) gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCAGCGCTACAAAAATATTAGAAACTGCAGCAGGTttgatatca > 1:2664092/1‑145 (MQ=11) ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttgag < 1:756399/150‑1 (MQ=32) tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCTGGTTTGAGTTGAGCGCAg > 2:2220012/1‑150 (MQ=17) tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATACGAACCTGCAGCAGGTTTGAGTTGATCGCAg > 2:2336167/1‑150 (MQ=17) aGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCg > 2:3375038/1‑150 (MQ=32) ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGaa > 2:1558787/1‑150 (MQ=32) agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaacaac < 2:1864027/150‑1 (MQ=32) ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCg > 2:537447/1‑150 (MQ=32) cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcg > 1:3650805/1‑150 (MQ=32) ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc > 2:1830447/1‑150 (MQ=32) ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc > 1:1029101/1‑150 (MQ=32) tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTGTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGAc > 2:328606/1‑150 (MQ=17) tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATATGCGAAACACAAATATACGAGCCAGCAGCAGCATTGAGATGAGCTCAGCGAAAACAAGGAGCCGCTCGCGGCGAc > 1:1079859/1‑150 (MQ=11) cAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACg > 1:2808406/1‑150 (MQ=32) aGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgc > 1:2668591/1‑150 (MQ=32) gTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATGTGCGATACACAAATATACGAACATGCGGCAGGATTGAGTTGAGAGAACCGAAACAACTGAGCCGCTCGCGGCGACGGCCCGAAGGgcga > 1:2802391/1‑150 (MQ=11) ttCGACTCCTGCTCGCCCCACCCCGGTCACCACGCGCCCTACAGGCGCTTTTTGGCTCTCTGCGATCCTCAACGATTCGACCCTGCAGCTGGTGTGAGTGGAGGGCCGCGACACTCCGGAGCCGCGCGCGGCGACGGCCCGAAGGgcgag < 2:812116/150‑1 (MQ=2) ttCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgag > 1:1030020/1‑150 (MQ=32) aTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaag > 1:669745/1‑150 (MQ=32) aTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaag > 1:1093013/1‑150 (MQ=32) tCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc > 2:2892466/1‑150 (MQ=32) tCCGGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCCGGTTTGTGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc < 2:1917987/150‑1 (MQ=255) accTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc > 1:3454439/2‑150 (MQ=17) cTGCACGACCCTCCAATGTAAAAAAGCGCCCTTAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCTGTTTTGAGTTGTGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcga < 2:2074298/150‑1 (MQ=11) cTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcga < 2:272325/150‑1 (MQ=32) tGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAg < 2:3659653/150‑1 (MQ=32) aCGACCCTCCACTGTCTTATAGCGCCGTATAGGCGCTTTTTTGCTATCTGCGATTCTCAAAGTTTCGAACATGCAGCAGGTTTGAGTTGAGCGCAGCGTAACTACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCa < 2:1699205/150‑1 (MQ=11) ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACACCAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTg > 2:2052895/1‑150 (MQ=17) caccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATAGTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGc > 2:2476110/1‑150 (MQ=17) accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa > 2:3307784/1‑150 (MQ=32) accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa > 2:3307788/1‑150 (MQ=32) ccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATGCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGCAGGGCGAGCGACGCGAGTCCTCCGGCAc > 2:2733670/1‑150 (MQ=11) caaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATATGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCCAAACAACGGAGCCGCACGCGGCGACGGCCCCAAGGGCGAGCCAAGCGAGTCATCCAGCcccaccca > 1:3264670/2‑150 (MQ=11) aaaaaaaGCGCCCTAACGGCTCTTTTTTGCTTTCTGCCATACTCACAGACTCGAACCTGCAGCGGTTTTGCTTTGAGCGCAGCTAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACcca < 1:3375469/149‑1 (MQ=11) aaaaaaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac > 2:3792869/1‑150 (MQ=32) aaaaaaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac > 1:477442/1‑150 (MQ=32) aaaaaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc > 1:2163830/1‑150 (MQ=32) aaaGCCCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa < 2:3018401/150‑1 (MQ=17) ccTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaa > 2:2509870/1‑150 (MQ=32) tAACGGCGCTTTTTTGCTTTCTGCTATACTAAAATTTTCGACCGTGCAGCTGGTTTGAGTTGTGCGCAGGGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaaa < 1:3317940/150‑1 (MQ=11) tAAAGGCTTTTTTTTGCTTTCGGCTATACTCAAATTTTGGAACGTGCAGCTGTTTTGTTTTGCGCGCAGCTAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaaa < 1:1558787/150‑1 (MQ=11) | GTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCG > NC_000913/781523‑781821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |