Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 832,378 G→T 5.2% intergenic (+142/+90) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913832,3780GT 5.2% 849.7 / 23.0 232intergenic (+142/+90)rhlE/ybiAATP‑dependent RNA helicase/DUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base G (103/117);  new base T (2/10);  total (105/127)
Fisher's exact test for biased strand distribution p-value = 7.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 256 total aligned reads displayed.

CGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCT  >  NC_000913/832229‑832526
                                                                                                                                                     |                                                                                                                                                    
cgcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                                      <  1:2430523/150‑1 (MQ=255)
cgcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                                      <  1:4049315/150‑1 (MQ=255)
cgcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                                      <  2:2974806/150‑1 (MQ=255)
cgcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                                      <  1:827055/150‑1 (MQ=255)
 gcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                                     <  1:3854621/150‑1 (MQ=255)
 gcAGTAATCTTTTATGCCGGGCTATGCCCGGCAACAGGCTGATGAACAAACGCAAATCTGCCTGATGCGCTACGCTTATAAGGCCTACGTGAACTCTGCAATATATTGAATTTTCATGCTTTTGTTGGCCGGATAAGGCGTTTTagccgc                                                                                                                                                     >  1:3154530/1‑150 (MQ=25)
       atattttttGCGGGGCTTTGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACGCTGCAATATATTGATTTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                               <  1:1758459/147‑1 (MQ=21)
       aTCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                               >  1:617920/1‑150 (MQ=255)
       aTCTTTTAGGCCGGGCTATGCCCGGCATCAGGCTGCTGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGAAATATATTTAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                               <  1:1974592/150‑1 (MQ=255)
        tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                              <  2:1105874/150‑1 (MQ=21)
        tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                              <  2:603067/150‑1 (MQ=21)
        tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATGTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                              <  2:1867958/150‑1 (MQ=21)
        tCTTTTATGCCGGGCGATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                              <  2:2807770/150‑1 (MQ=21)
          ttttATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt                                                                                                                                            >  2:2135082/1‑150 (MQ=255)
          ttttATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt                                                                                                                                            <  1:2528794/150‑1 (MQ=255)
          ttttATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt                                                                                                                                            >  1:2385714/1‑150 (MQ=255)
          ttttATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTAAGCTTATCTGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTGGTCGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt                                                                                                                                            >  2:3662737/1‑150 (MQ=255)
            tgaTGCCGGGCATAGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt                                                                                                                                          >  2:3623374/3‑150 (MQ=255)
            tgaTGCCGGGCATAGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt                                                                                                                                          >  2:1780792/3‑150 (MQ=255)
               tGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTca                                                                                                                                        >  2:2765680/1‑149 (MQ=255)
               tGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTca                                                                                                                                        <  1:2765680/149‑1 (MQ=255)
                gCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcaca                                                                                                                                      >  2:1593550/1‑150 (MQ=255)
                 ccGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTTAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCAACCGGCAATTCacaa                                                                                                                                     >  1:2234366/1‑150 (MQ=255)
                 ccGGGCTATGCCCGGAATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCacaa                                                                                                                                     <  1:531025/150‑1 (MQ=255)
                    ggCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCAcaacaa                                                                                                                                  >  1:3432469/1‑150 (MQ=255)
                    ggCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCAcaacaa                                                                                                                                  <  1:1310485/150‑1 (MQ=255)
                     gCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg                                                                                                                                 <  2:3443737/150‑1 (MQ=255)
                       tATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCa                                                                                                                               >  2:3178130/1‑150 (MQ=255)
                          gCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACtt                                                                                                                            <  2:286644/150‑1 (MQ=255)
                                       cTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTga                                                                                                               <  1:1017101/150‑1 (MQ=255)
                                       cTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTga                                                                                                               <  1:871264/150‑1 (MQ=255)
                                       cTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTga                                                                                                               <  1:2852566/150‑1 (MQ=255)
                                        tgatgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTgag                                                                                                              >  2:3665768/1‑150 (MQ=255)
                                          atgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTACAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGaa                                                                                                            <  2:3019346/150‑1 (MQ=255)
                                             aaCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAacac                                                                                                         <  1:3052625/150‑1 (MQ=255)
                                              aCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACg                                                                                                        <  1:3584300/150‑1 (MQ=255)
                                                aaaCGCAAAACTGCATGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATAGTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTAGTCAGCAATTTGAGAACACggg                                                                                                      >  2:1347683/1‑150 (MQ=255)
                                                 aaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGa                                                                                                     >  1:2445528/1‑150 (MQ=255)
                                                  aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATATGAGAACACGGGaa                                                                                                    >  2:3582349/1‑150 (MQ=255)
                                                        aaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAAtt                                                                                              <  2:543125/150‑1 (MQ=255)
                                                         aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAAttt                                                                                             <  1:2629220/150‑1 (MQ=255)
                                                           tcccTGATGCGCTACGCTTATCAGGCCTACGAGAACTCTGCAATATAATGAATTTGCAAGCTTTTGTAGGCCGGATAACGCGTTAACCCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACTGGAAAAAATTTAt                                                                                           >  2:2283637/3‑150 (MQ=21)
                                                           tGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTAt                                                                                           <  2:3704213/150‑1 (MQ=255)
                                                              cTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTg                                                                                        <  2:1881144/150‑1 (MQ=255)
                                                                    cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTTTCTGTTTTAc                                                                                  <  2:2823593/150‑1 (MQ=255)
                                                                    cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAc                                                                                  <  1:1832963/150‑1 (MQ=255)
                                                                     gcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAcc                                                                                 <  2:2908127/150‑1 (MQ=255)
                                                                       tACGCTTATCATGACTACGTGAACTCTGCAATATATTGATTTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACATGCACTTGTCAGCAATTTGAGATCACGGGAAAAAATTTATCTGTTTTACCGc                                                                               <  2:3111285/150‑1 (MQ=25)
                                                                        aCGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGTAAAAAATTTATCTGTTTTACCGcc                                                                              <  1:2536546/150‑1 (MQ=255)
                                                                        aCGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGcc                                                                              <  2:2615367/150‑1 (MQ=255)
                                                                             tATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATaa                                                                         <  1:3771355/150‑1 (MQ=255)
                                                                             tATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATaa                                                                         <  1:2869844/150‑1 (MQ=255)
                                                                             tATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATaa                                                                         <  1:1659257/150‑1 (MQ=255)
                                                                             tATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATaa                                                                         <  1:1580846/150‑1 (MQ=255)
                                                                              aTCAGGCCTACGTGAACTCTGCAATATCTTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATacg                                                                        <  2:2377512/150‑3 (MQ=255)
                                                                               tCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGc                                                                       >  1:2048778/1‑150 (MQ=255)
                                                                                  ggCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCAc                                                                    >  2:3771649/1‑150 (MQ=255)
                                                                                   gCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCAcc                                                                   >  1:3374740/1‑150 (MQ=255)
                                                                                       aCGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCCCAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgaga                                                               <  2:501265/150‑1 (MQ=255)
                                                                                          tGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGt                                                            <  2:82968/150‑1 (MQ=255)
                                                                                          tGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGt                                                            >  2:1725598/1‑150 (MQ=255)
                                                                                          tGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGACGCATCCGGCATATCACAACAAGCACTTGTCAGCAATTAGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGt                                                            >  2:251079/1‑150 (MQ=255)
                                                                                               tctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACtt                                                       >  1:3401512/1‑150 (MQ=255)
                                                                                                 tGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtct                                                     >  2:1513163/1‑150 (MQ=255)
                                                                                                 tGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtct                                                     >  2:1409176/1‑150 (MQ=255)
                                                                                                     atatatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTATACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGACAAAATTTATCTGTTTTACCGCCCCTACGCCACCGCGACGTTCCGTCTCtat                                                 >  2:544429/1‑150 (MQ=37)
                                                                                                      tatctTGAATTTGCATGCTTGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCtata                                                <  1:3265306/150‑1 (MQ=255)
                                                                                                      tatatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGACAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCtata                                                >  1:2696466/1‑150 (MQ=255)
                                                                                                      tatatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCtata                                                >  2:3885034/1‑150 (MQ=255)
                                                                                                      tatatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCtata                                                <  1:3265591/150‑1 (MQ=255)
                                                                                                         atTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGcc                                             <  2:4120957/150‑1 (MQ=255)
                                                                                                          ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCa                                            >  1:1213411/1‑150 (MQ=255)
                                                                                                           tGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCaa                                           <  1:2035021/150‑1 (MQ=255)
                                                                                                           tGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCaa                                           >  1:154143/1‑150 (MQ=255)
                                                                                                            gAATTTGCAAGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACAGCCCATAAGCCACCGAGACGTTACTTCTCTATAGACAAt                                          >  1:806573/1‑150 (MQ=255)
                                                                                                             aaTTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAAtt                                         <  1:3315039/150‑1 (MQ=255)
                                                                                                                ttGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGtt                                      >  1:1494462/1‑150 (MQ=255)
                                                                                                                gtGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGtt                                      <  2:26456/149‑1 (MQ=255)
                                                                                                                  gCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTcg                                    <  2:2591323/150‑1 (MQ=255)
                                                                                                                   cATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTcgc                                   <  1:3721449/150‑1 (MQ=255)
                                                                                                                   cATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTcgc                                   >  2:1510759/1‑150 (MQ=255)
                                                                                                                        ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCGCCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAAc                              >  1:2019444/1‑150 (MQ=255)
                                                                                                                        ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAAc                              >  1:3778284/1‑150 (MQ=255)
                                                                                                                        ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAAc                              >  1:3989702/1‑150 (MQ=255)
                                                                                                                        ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGGTACTGCTCTAGAGCCACTGGTTCGAGCAAc                              >  2:3986884/1‑150 (MQ=38)
                                                                                                                        ttttGTAGGCCGGATAAGGCGATTACGCCGCATCCGGCAAATCACAACAAGCACTTGACAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAAc                              <  1:1671740/150‑1 (MQ=255)
                                                                                                                             tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCAt                         <  2:3154499/150‑1 (MQ=255)
                                                                                                                               ggCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTa                       >  1:2341114/1‑150 (MQ=255)
                                                                                                                                 ccGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTaaa                     >  1:3010001/1‑150 (MQ=255)
                                                                                                                                  cGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGACAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATGGTTCGCGCCACTCCAGTcaaa                    >  2:129203/1‑150 (MQ=255)
                                                                                                                                  cGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTaaaa                    >  1:3188006/1‑150 (MQ=255)
                                                                                                                                  cGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTaaaa                    <  1:447278/150‑1 (MQ=255)
                                                                                                                                         ggCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCATCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGcc             <  1:1111778/150‑1 (MQ=255)
                                                                                                                                          gCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGccc            >  2:2204151/1‑150 (MQ=255)
                                                                                                                                              ttACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTc        <  1:3574071/150‑1 (MQ=255)
                                                                                                                                                 cgccgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTAGCGCAACTCCATTAAAAGGTAGCCCAGTCTAt     >  2:3271777/1‑150 (MQ=255)
                                                                                                                                                  gccgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGAtt    >  2:3945081/1‑150 (MQ=255)
                                                                                                                                                  gccgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGAtt    <  2:3151489/150‑1 (MQ=255)
                                                                                                                                                   ccgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTc   >  1:651988/1‑150 (MQ=255)
                                                                                                                                                    cgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCt  >  2:692209/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCT  >  NC_000913/832229‑832526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: