Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,985,112 C→G 5.5% intergenic (‑43/+27) araG ← / ← araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,985,1120CG 5.5% 599.5 / 21.4 165intergenic (‑43/+27)araG/araFL‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (75/80);  new base G (5/4);  total (81/84)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 174 total aligned reads displayed.

GAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAA  >  NC_000913/1984964‑1985260
                                                                                                                                                    |                                                                                                                                                    
gAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGcatca                                                                                                                                                     <  1:1440442/150‑1 (MQ=255)
  aCCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATg                                                                                                                                                   <  1:1786894/150‑1 (MQ=255)
        ccGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATGATGCAGAgg                                                                                                                                             <  2:1234288/150‑1 (MQ=255)
         cGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAggg                                                                                                                                            >  2:3096325/1‑150 (MQ=255)
            cATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATGATGCAGAgggggt                                                                                                                                         <  2:1837892/150‑3 (MQ=255)
             aTAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTATTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAAt                                                                                                                                        >  1:572938/1‑150 (MQ=255)
                 cAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAAttttt                                                                                                                                    <  2:1235053/150‑1 (MQ=255)
                    tCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCg                                                                                                                                 >  1:858625/1‑150 (MQ=255)
                    tCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCg                                                                                                                                 >  2:1790304/1‑150 (MQ=255)
                    tCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCg                                                                                                                                 >  2:3697555/1‑150 (MQ=255)
                        aaCTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGTGGGGAATTTTTCCGGCaa                                                                                                                             <  2:3388746/150‑1 (MQ=255)
                          cTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAt                                                                                                                           <  1:4003058/150‑1 (MQ=255)
                                tCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttactta                                                                                                                     <  2:2109717/150‑1 (MQ=255)
                                 ccGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttacttac                                                                                                                    >  2:823152/1‑150 (MQ=255)
                                  cGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTAcc                                                                                                                   <  1:3850225/150‑1 (MQ=255)
                                   gTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCg                                                                                                                  >  1:319844/1‑150 (MQ=255)
                                     cAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGcc                                                                                                                <  1:434800/150‑1 (MQ=255)
                                          ccTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAc                                                                                                           <  2:3229105/150‑1 (MQ=255)
                                           cTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAcc                                                                                                          >  2:2586162/1‑150 (MQ=255)
                                           cTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAcc                                                                                                          >  1:2303740/1‑150 (MQ=255)
                                           cTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAcc                                                                                                          >  2:3885496/1‑150 (MQ=255)
                                            ttAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCt                                                                                                         >  1:466275/1‑150 (MQ=255)
                                            ttAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCt                                                                                                         >  1:1291501/1‑150 (MQ=255)
                                              aaCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttt                                                                                                       <  2:652820/150‑1 (MQ=255)
                                              aaCGCCGGGAAACGTTTGACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGACTCACTGGTCGTTCACACCCCCTCTGCATCAAGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttt                                                                                                       <  2:346853/150‑1 (MQ=255)
                                               aCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTCCCGCCTAAACCtttt                                                                                                      >  2:3913789/1‑150 (MQ=255)
                                                cGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttttt                                                                                                     <  1:1936037/150‑1 (MQ=255)
                                                cGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttttt                                                                                                     <  1:1606164/150‑1 (MQ=255)
                                                   cGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtct                                                                                                  >  2:2175213/1‑150 (MQ=255)
                                                   cGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtct                                                                                                  <  2:3736251/150‑1 (MQ=255)
                                                    gggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtctc                                                                                                 <  2:3104021/150‑1 (MQ=255)
                                                    gggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtctc                                                                                                 <  2:1117455/150‑1 (MQ=255)
                                                    gggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTAGCATAACGTCTCCGTGAATCACTGGTCGATCACACCCCATCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtctc                                                                                                 >  2:273286/1‑150 (MQ=255)
                                                     ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTcc                                                                                                >  1:3640210/1‑150 (MQ=255)
                                                           gTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTATTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAAATTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttct                                                                                          <  2:572938/150‑1 (MQ=255)
                                                            ttttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAGTACTTACCGCCTAAACCTTTTTTCTCCAGttctt                                                                                         <  2:1682974/150‑1 (MQ=255)
                                                                 ccGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTa                                                                                    <  1:2918932/150‑1 (MQ=255)
                                                                   gATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                  <  1:947025/150‑1 (MQ=255)
                                                                   gATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                  <  2:1197516/150‑1 (MQ=255)
                                                                   gATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                  <  1:714472/150‑1 (MQ=255)
                                                                   gATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                  <  2:1197493/150‑1 (MQ=255)
                                                                   gATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                  <  1:2000090/150‑1 (MQ=255)
                                                                    aTGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAATTTACTTACCGCCTAATCCTTTTTTCTCCAGTTGTTCTTTAAAg                                                                                 >  2:4039034/1‑150 (MQ=255)
                                                                       ccGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTg                                                                              >  1:2911394/1‑150 (MQ=255)
                                                                         gcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc                                                                            <  2:280603/150‑1 (MQ=255)
                                                                         gcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc                                                                            >  1:3989112/1‑150 (MQ=255)
                                                                             aaTGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCAcgc                                                                        <  1:1142286/150‑1 (MQ=255)
                                                                             aaTGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCAcgc                                                                        <  2:3999361/150‑1 (MQ=255)
                                                                              aTGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCAcgcg                                                                       >  1:3229704/1‑150 (MQ=255)
                                                                                gagagaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTg                                                                     <  1:2518064/150‑1 (MQ=255)
                                                                                 agagaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGa                                                                    >  2:123167/1‑150 (MQ=255)
                                                                                  gagaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGAt                                                                   <  2:3314484/150‑1 (MQ=255)
                                                                                  gagaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGAt                                                                   <  2:1250354/150‑1 (MQ=255)
                                                                                       aCGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTa                                                              >  2:3549977/1‑150 (MQ=255)
                                                                                        cGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCATATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTAc                                                             <  2:2252318/150‑1 (MQ=255)
                                                                                        cGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTAc                                                             <  2:3060185/150‑1 (MQ=255)
                                                                                         ggggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTAcc                                                            >  1:3399336/1‑150 (MQ=255)
                                                                                          gggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCa                                                           <  1:785384/150‑1 (MQ=255)
                                                                                             tAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGt                                                        <  1:638649/150‑1 (MQ=255)
                                                                                              aGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTc                                                       <  1:1344809/150‑1 (MQ=255)
                                                                                                aCTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCgg                                                     >  1:1019846/1‑150 (MQ=255)
                                                                                                aCTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCgg                                                     >  2:2313055/1‑150 (MQ=255)
                                                                                                   gTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTaa                                                  <  1:3558178/150‑1 (MQ=255)
                                                                                                   gTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTaa                                                  >  1:3174411/1‑150 (MQ=255)
                                                                                                   gTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTaa                                                  <  1:3712827/150‑1 (MQ=255)
                                                                                                    ttGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAAc                                                 <  2:237886/150‑1 (MQ=255)
                                                                                                      gCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACtt                                               <  2:319844/150‑1 (MQ=255)
                                                                                                        aTAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCg                                             <  1:2519295/150‑1 (MQ=255)
                                                                                                         tAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGGAGAGGGGAATTTTTCCGGCAAATTTCTTACCGGCTAAACCTTTTTTGTGCAGTTGTTGTTTAATGTTGTCACGCGTGATCAGTACCACGTCGGTAAGTTCgg                                            >  1:2948016/1‑150 (MQ=255)
                                                                                                         tAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCgg                                            >  1:1007890/1‑150 (MQ=255)
                                                                                                         tAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCgg                                            >  1:2076655/1‑150 (MQ=255)
                                                                                                                 ccGTGACTCACGGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtttt                                    <  2:2668082/150‑1 (MQ=255)
                                                                                                                 ccGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtttt                                    <  2:2668096/150‑1 (MQ=255)
                                                                                                                    tGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGc                                 <  1:2897707/150‑1 (MQ=255)
                                                                                                                    tGAATCACTGGTCGGTCACACCCCCTCTGCATCATGCAGAGGGGAATGTGTCCGGCAAAGTACTTACCGCCTAAACCTTTTTTCTCCCGTTCGTCTTTCAAGTTGTCACGCGGGATCAGTACCACGTCGGTAACTGCGGTCCATTTTGGc                                 >  2:399613/1‑150 (MQ=255)
                                                                                                                     gAATCACTGGTCGTTCACACCCCCACTGCATCATGCAGAGGGGAATTTTTCCGGCAAAATACTTACCGCCTAAACCATTTTTATCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCg                                >  1:1188209/1‑150 (MQ=255)
                                                                                                                       aTCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGt                              <  1:534031/150‑1 (MQ=255)
                                                                                                                        tCACTGGTCGTTCACACCCCCACTGCAAAATGCAGAGGGGAAATTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTACTTCTTTAAAGTTGTCACGCGTGATAAGTAACACGACGGGAAAGTCGGTAAAATTTGTGGGtt                             >  1:3288240/1‑150 (MQ=255)
                                                                                                                         cACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTc                            <  2:1364945/150‑1 (MQ=255)
                                                                                                                            tGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAAc                         <  2:200849/150‑1 (MQ=255)
                                                                                                                              gTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGt                       >  2:1168353/1‑150 (MQ=255)
                                                                                                                               tCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTc                      >  2:2336985/1‑150 (MQ=255)
                                                                                                                               tCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTc                      >  1:9385/1‑150 (MQ=255)
                                                                                                                               tCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCGTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTc                      >  1:3949761/1‑150 (MQ=255)
                                                                                                                                  ttCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCCGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCttt                   >  2:448928/1‑150 (MQ=255)
                                                                                                                                   tCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCtttt                  <  1:3096325/150‑1 (MQ=255)
                                                                                                                                          cccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAccc           >  2:1507295/1‑150 (MQ=255)
                                                                                                                                          cccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAccc           >  2:3214309/1‑150 (MQ=255)
                                                                                                                                          cccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAccc           >  1:3254582/1‑150 (MQ=255)
                                                                                                                                          cccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGGTCAACGTCGTTTGCTAccc           >  1:2218686/1‑150 (MQ=255)
                                                                                                                                           ccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCa          >  2:3238275/1‑150 (MQ=255)
                                                                                                                                           ccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCa          >  1:4069938/1‑150 (MQ=255)
                                                                                                                                           ccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCa          <  2:557363/150‑1 (MQ=255)
                                                                                                                                           ccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCa          <  2:578889/150‑1 (MQ=255)
                                                                                                                                                 catgatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTa    >  1:2952931/1‑150 (MQ=255)
                                                                                                                                                 catcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTa    <  1:719464/150‑1 (MQ=255)
                                                                                                                                                 catcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTa    <  2:2911394/150‑1 (MQ=255)
                                                                                                                                                 catcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTa    <  2:2909389/150‑1 (MQ=255)
                                                                                                                                                  atcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGGTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTaa   <  1:1630354/150‑1 (MQ=255)
                                                                                                                                                   tcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGTCGGTTCAACGTCTTTTGCTACCCAGTTGTaat  >  2:3886238/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAA  >  NC_000913/1984964‑1985260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: