Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,167,278 G→A 5.4% intergenic (+78/+24) cyaR → / ← ogrK sRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,167,2780GA 5.4% 840.2 / 23.3 241intergenic (+78/+24)cyaR/ogrKsRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth
Reads supporting (aligned to +/- strand):  ref base G (127/101);  new base A (5/8);  total (132/109)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 248 total aligned reads displayed.

CCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTT  >  NC_000913/2167129‑2167427
                                                                                                                                                     |                                                                                                                                                     
ccATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAATAGCCCCTCg                                                                                                                                                       >  2:2788673/1‑150 (MQ=255)
 cATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGa                                                                                                                                                      >  2:1619119/1‑150 (MQ=255)
 cATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGa                                                                                                                                                      >  2:2448639/1‑150 (MQ=255)
        tGCTAGCTTTACCCGGAACCACCTCCTTCGCGTTTGTAATCTCCCTCACACGGGCTTATTTTTTCCGCTTAATACAATTAAAAAAAAGGATTTTTTTCTGGTCACGTCCAAACATTGACCACATCGACAAAAAAGCCCCTCGACTGAggg                                                                                                                                               <  1:735468/150‑1 (MQ=255)
         gCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAgggg                                                                                                                                              <  1:2325114/150‑1 (MQ=255)
           tAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCTTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCt                                                                                                                                            <  1:130424/150‑1 (MQ=255)
           tAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCCCATCGACCAAAACGCCCCTCGACTGAGGGGCt                                                                                                                                            >  1:2121113/1‑150 (MQ=255)
           tAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCt                                                                                                                                            >  2:2077873/1‑150 (MQ=255)
            aGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCtt                                                                                                                                           <  2:3946257/150‑1 (MQ=255)
             gCTGTCCCCGGAACCACCGCCTTAGCCGGTGTAATCTCCCTTACCCGGGCTTATTTTTTACGCGTAATACAATTAAATAAAAGGATTTAGTTCTGGTCACGTCAACACTTTGACCACATCGACAAACAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                          <  1:3914430/150‑2 (MQ=255)
             gCTGTACCAGGAACCCCCTCCTTAGCCTGTTTAATCTCCCTTACACGGGCTTATTTTTTCCGCGTAATACAATGAAATAAAAGGATTTATTTCTGGACACGCCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                          <  1:1399109/150‑2 (MQ=255)
             gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                                                                                                                                          >  2:3968054/1‑150 (MQ=255)
             gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                          <  1:2684406/150‑2 (MQ=255)
               tGTACCAGGAACCACCTCCTTCGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATTAAATAAAAGTATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCt                                                                                                                                        <  1:3786675/150‑1 (MQ=255)
               tGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAAAAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCt                                                                                                                                        <  1:1218314/150‑1 (MQ=255)
                 tACCAGGAACCACCTCCTTCGCCTGTTTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGt                                                                                                                                      <  1:3760642/150‑1 (MQ=255)
                 tACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGTGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGt                                                                                                                                      >  2:3532974/1‑150 (MQ=255)
                 tACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGt                                                                                                                                      <  1:952196/150‑1 (MQ=255)
                   ccAGGCCCCACCTCCTTCGCCTGTGTACGCTCCCGGCCCCGGGCGTATTTTTGCCGCGTAATACCCTGCACTACCAGGATTTATTTCTGGTCACGGCCACACATTGACCACATCGACCAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                    <  2:163230/150‑1 (MQ=255)
                   ccAGGACCCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGATTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                    <  1:1039889/150‑1 (MQ=255)
                    cAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTc                                                                                                                                   >  1:3037371/1‑150 (MQ=255)
                       gAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTaa                                                                                                                                >  2:2769066/1‑150 (MQ=255)
                       gAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACAGAGGGGCGTACTGTTTCTaa                                                                                                                                >  2:339710/1‑150 (MQ=255)
                         accaccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAtt                                                                                                                              <  1:1138073/150‑1 (MQ=255)
                           caccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTAc                                                                                                                            <  2:83041/150‑1 (MQ=255)
                              ctcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATc                                                                                                                         >  1:1767122/1‑150 (MQ=255)
                                cctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCTCATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCca                                                                                                                       <  2:3474961/150‑1 (MQ=255)
                                 ctTAGCCTGTGTAATCTCCCTTACCCGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCcac                                                                                                                      <  1:2625410/150‑1 (MQ=255)
                                        tgtgTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATACttt                                                                                                               >  2:198865/1‑150 (MQ=255)
                                         gtgtAATCTCCCTTACACGGGCTTATTTATTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtg                                                                                                              <  1:1430843/150‑1 (MQ=255)
                                          tgtAATCTCCCTTACACGGGCTTATTTTTTACGTGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                             <  1:3532974/150‑1 (MQ=255)
                                          tgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                             <  2:770388/150‑1 (MQ=255)
                                                  cccTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATATAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGa                                                                                                     <  2:1710325/150‑1 (MQ=255)
                                                    cTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                   <  2:2685874/150‑1 (MQ=255)
                                                    cTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                   <  2:1829236/150‑1 (MQ=255)
                                                    cTTACACGGGCTTATTTTTTACGCGTAATACAAAGAAATAAAAGGATTTATTTCTGGACACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                   <  2:3984747/150‑1 (MQ=255)
                                                     ttACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACgg                                                                                                  <  1:2725761/150‑1 (MQ=255)
                                                       acacGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCa                                                                                                >  2:315089/1‑150 (MQ=255)
                                                         acGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAAc                                                                                              >  2:810820/1‑150 (MQ=255)
                                                          cGGGCTTATTTTTTACGCGTAATACAATGAAATAACAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACg                                                                                             <  1:3601329/150‑1 (MQ=255)
                                                           gggCTTATTTTTTACGCGTAATACAATGACATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACgg                                                                                            <  2:1326701/150‑1 (MQ=255)
                                                           gggCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTGATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACgg                                                                                            <  1:2980619/150‑1 (MQ=255)
                                                             gCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGt                                                                                          <  1:1937321/150‑1 (MQ=255)
                                                               ttATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGc                                                                                        >  1:347654/1‑150 (MQ=255)
                                                               ttATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGc                                                                                        >  1:3324072/1‑150 (MQ=255)
                                                                tATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCACCGGGTGCg                                                                                       >  2:4066091/1‑150 (MQ=255)
                                                                   tttttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGcc                                                                                    <  1:2769066/150‑1 (MQ=255)
                                                                     tttACGCGTAATCCAATGAAATAAAATGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCtt                                                                                  <  2:858702/150‑1 (MQ=255)
                                                                      ttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACCTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTa                                                                                 >  2:3222801/1‑150 (MQ=255)
                                                                          gcgTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAATACATCCACATAATATGCTGCCCTGACGGCAACGGGTGCGGCCATacgac                                                                             >  2:3412094/1‑150 (MQ=255)
                                                                            gTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGt                                                                           <  1:1784689/150‑1 (MQ=255)
                                                                             tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                          >  2:1160594/1‑150 (MQ=255)
                                                                             tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                          <  1:3022075/150‑1 (MQ=255)
                                                                             tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                          <  2:3489081/150‑1 (MQ=255)
                                                                             tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                          <  2:1796228/150‑1 (MQ=255)
                                                                              aaTACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTgg                                                                         <  1:272552/150‑1 (MQ=255)
                                                                                    aTGAAATAAAATGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtct                                                                   <  1:15474/150‑1 (MQ=255)
                                                                                     tGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtctc                                                                  >  1:417024/1‑150 (MQ=255)
                                                                                     tGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtctc                                                                  <  1:1777484/150‑1 (MQ=255)
                                                                                     tGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtctc                                                                  <  1:1584914/150‑1 (MQ=255)
                                                                                      gAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTcc                                                                 >  2:2179878/1‑150 (MQ=255)
                                                                                      gAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTcc                                                                 >  2:2867071/1‑150 (MQ=255)
                                                                                       aaaTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTccc                                                                <  1:1110354/150‑1 (MQ=255)
                                                                                       aaaTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTccc                                                                >  1:3422250/1‑150 (MQ=255)
                                                                                            aaaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCtt                                                           <  2:3037371/150‑1 (MQ=255)
                                                                                             aaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTc                                                          >  2:329798/1‑150 (MQ=255)
                                                                                             aaGGATTTATTTCTGGTCACGTCCACACATAGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCAGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTc                                                          >  2:1511426/1‑150 (MQ=255)
                                                                                              aGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTca                                                         <  1:3969846/150‑1 (MQ=255)
                                                                                               ggATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcac                                                        >  1:1186873/1‑150 (MQ=255)
                                                                                                gATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcaca                                                       <  2:469546/150‑1 (MQ=255)
                                                                                                gATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcaca                                                       >  1:1982243/1‑150 (MQ=255)
                                                                                                  tttatttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAAt                                                     <  1:3051469/150‑1 (MQ=255)
                                                                                                       ttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATc                                                >  1:2224836/1‑150 (MQ=255)
                                                                                                          tGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACTTCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACATGGACTTCTCCCGGCTTCACAATGTATCGCt                                             >  1:3657260/1‑150 (MQ=255)
                                                                                                                  tCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGAc                                     >  2:2385034/1‑150 (MQ=255)
                                                                                                                    cacacaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTc                                   >  2:89431/1‑150 (MQ=255)
                                                                                                                     acacaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCa                                  >  2:2234281/1‑150 (MQ=255)
                                                                                                                         aTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAg                              >  2:1699214/1‑150 (MQ=255)
                                                                                                                           tGACCACATCGACAAAACAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtg                            <  1:3186145/150‑1 (MQ=255)
                                                                                                                             aCCACATCGACAAAATAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACACTGTCTCGCTGTACCGACTCATAAGtgat                          >  2:4030963/1‑150 (MQ=255)
                                                                                                                                acaTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGaa                       >  2:1253059/1‑150 (MQ=255)
                                                                                                                                acaTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGaa                       <  1:2008282/150‑1 (MQ=255)
                                                                                                                                 caTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAAc                      >  1:1347557/1‑150 (MQ=255)
                                                                                                                                     gACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTgg                  <  1:3648352/150‑1 (MQ=255)
                                                                                                                                     gACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTgg                  >  2:1426695/1‑150 (MQ=255)
                                                                                                                                     gACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTgg                  >  2:2660611/1‑150 (MQ=255)
                                                                                                                                        aaaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATCATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcgc               <  1:2151969/150‑1 (MQ=255)
                                                                                                                                        aaaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcgc               >  2:3704312/1‑150 (MQ=255)
                                                                                                                                        aaaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcgc               >  2:4007673/1‑150 (MQ=255)
                                                                                                                                          ggaaGCCCCTCAGTCGAGGGGCTTTCTGTTTCTAATTAAATACACAAAATTTGCTGCCCTGACGGCAACGGGTGCGGCCATACGACGTGGACTACTCCCGGCTTCAAAATGTATCGCTGTACCGACTCATAAGAGATGAACGTGGCGCTg             >  1:644995/3‑150 (MQ=255)
                                                                                                                                          ggaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTg             >  2:422178/3‑150 (MQ=255)
                                                                                                                                           aaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGc            <  2:651262/150‑1 (MQ=255)
                                                                                                                                           aaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATAGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGc            >  1:3947507/1‑150 (MQ=255)
                                                                                                                                                cccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTc       <  1:690343/150‑1 (MQ=255)
                                                                                                                                                cccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACATCTCCCGGCTTCACAAAGTATCGCAGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTc       >  1:1932121/1‑150 (MQ=255)
                                                                                                                                                 ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCa      <  2:3639039/150‑1 (MQ=255)
                                                                                                                                                 ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCa      >  1:1679498/1‑150 (MQ=255)
                                                                                                                                                  cTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCAc     >  2:483748/1‑150 (MQ=255)
                                                                                                                                                    cGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGt   <  2:2411413/150‑1 (MQ=255)
                                                                                                                                                     gACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGtt  >  2:2213185/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTT  >  NC_000913/2167129‑2167427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: