Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,167,279 A→G 5.4% intergenic (+79/+23) cyaR → / ← ogrK sRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,167,2790AG 5.4% 812.1 / 24.5 239intergenic (+79/+23)cyaR/ogrKsRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth
Reads supporting (aligned to +/- strand):  ref base A (124/101);  new base G (5/8);  total (130/109)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 244 total aligned reads displayed.

CATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTC  >  NC_000913/2167130‑2167428
                                                                                                                                                     |                                                                                                                                                     
cATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGa                                                                                                                                                       >  2:2448639/1‑150 (MQ=255)
     aaTGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTTTAATCTCCCTTACACGGGCTTATTTTTTACGCTTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAg                                                                                                                                                  <  1:2159273/150‑1 (MQ=255)
          tAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCTTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCt                                                                                                                                             <  1:130424/150‑1 (MQ=255)
          tAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCt                                                                                                                                             >  2:2077873/1‑150 (MQ=255)
            gCTGTACCCGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGATTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                           <  1:2684411/150‑2 (MQ=255)
            gCTGTACCAGGAACCCCCTCCTTAGCCTGTTTAATCTCCCTTACACGGGCTTATTTTTTCCGCGTAATACAATGAAATAAAAGGATTTATTTCTGGACACGCCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                           <  1:1399109/150‑2 (MQ=255)
            gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                                                                                                                                           >  2:3968054/1‑150 (MQ=255)
            gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                           <  2:2396425/150‑2 (MQ=255)
              tGTACCAGGAACCACCTCCTTCGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATTAAATAAAAGTATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCt                                                                                                                                         <  1:3786675/150‑1 (MQ=255)
              tGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAAAAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCt                                                                                                                                         <  1:1218314/150‑1 (MQ=255)
                tACCAGGAACCACCTCCTTCGCCTGTTTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGt                                                                                                                                       <  1:3760642/150‑1 (MQ=255)
                tACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGt                                                                                                                                       <  1:952196/150‑1 (MQ=255)
                  ccAGGCCCCACCTCCTTCGCCTGTGTACGCTCCCGGCCCCGGGCGTATTTTTGCCGCGTAATACCCTGCACTACCAGGATTTATTTCTGGTCACGGCCACACATTGACCACATCGACCAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                     <  2:163230/150‑1 (MQ=255)
                  ccAGGACCCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGATTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                     <  1:1039889/150‑1 (MQ=255)
                   cAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTc                                                                                                                                    >  1:1910098/1‑150 (MQ=255)
                   cAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTc                                                                                                                                    >  1:3037371/1‑150 (MQ=255)
                      gAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGGAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAACAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTaa                                                                                                                                 <  2:3448401/150‑1 (MQ=255)
                        accaccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAtt                                                                                                                               <  1:1138073/150‑1 (MQ=255)
                          caccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTAc                                                                                                                             <  2:83041/150‑1 (MQ=255)
                             ctcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATc                                                                                                                          >  1:1767122/1‑150 (MQ=255)
                                ctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCcac                                                                                                                       <  2:104874/150‑1 (MQ=255)
                                 ttAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCcaca                                                                                                                      >  1:2073461/1‑150 (MQ=255)
                                        gtgtAATCTCCCTTACACGGGCTTATTTATTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtg                                                                                                               <  1:1430843/150‑1 (MQ=255)
                                         tgtAATCTCCCTTACACGGGCTTATTTTTTACGTGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                              <  1:3532974/150‑1 (MQ=255)
                                         tgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGTGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                              >  1:2512841/1‑150 (MQ=255)
                                         tgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                              <  2:770388/150‑1 (MQ=255)
                                           tAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgctg                                                                                                            <  1:2134112/150‑1 (MQ=255)
                                             aTCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGcc                                                                                                          <  2:3952532/150‑1 (MQ=255)
                                                tcCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGGCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATACTTTGCTGCCCTg                                                                                                       >  1:3883016/1‑150 (MQ=255)
                                                   cTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                    >  1:722578/1‑150 (MQ=255)
                                                   cTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                    <  2:2685874/150‑1 (MQ=255)
                                                    ttACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACgg                                                                                                   <  1:2725761/150‑1 (MQ=255)
                                                      acacGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCa                                                                                                 >  2:315089/1‑150 (MQ=255)
                                                        acGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAAc                                                                                               >  2:810820/1‑150 (MQ=255)
                                                         cGGGCTTATTTTTTACGCGTAATACAATGAAATAACAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACg                                                                                              <  1:3601329/150‑1 (MQ=255)
                                                          gggCTTATTTTTTACGCGTAATACAATGACATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACgg                                                                                             <  2:1326701/150‑1 (MQ=255)
                                                          gggCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTGATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACgg                                                                                             <  1:2980619/150‑1 (MQ=255)
                                                            gCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGt                                                                                           <  1:1937321/150‑1 (MQ=255)
                                                              ttATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCACCGGGTGc                                                                                         >  1:3120411/1‑150 (MQ=255)
                                                              ttATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGc                                                                                         >  1:3324072/1‑150 (MQ=255)
                                                              ttATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGc                                                                                         >  1:347654/1‑150 (MQ=255)
                                                               tATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCACCGGGTGCg                                                                                        >  2:4066091/1‑150 (MQ=255)
                                                                aTTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCgg                                                                                       >  2:272552/1‑150 (MQ=255)
                                                                 ttttttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGc                                                                                      <  1:1993219/150‑1 (MQ=255)
                                                                  tttttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGcc                                                                                     >  2:3993966/1‑150 (MQ=255)
                                                                    tttACGCGTAATCCAATGAAATAAAATGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCtt                                                                                   <  2:858702/150‑1 (MQ=255)
                                                                     ttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACCTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTa                                                                                  >  2:3222801/1‑150 (MQ=255)
                                                                        cgcgTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTacga                                                                               <  1:280283/150‑1 (MQ=255)
                                                                           gTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGt                                                                            <  1:1784689/150‑1 (MQ=255)
                                                                            tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                           <  1:1970733/150‑1 (MQ=255)
                                                                            tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                           <  2:277170/150‑1 (MQ=255)
                                                                            tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                           <  1:1769330/150‑1 (MQ=255)
                                                                             aaTACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTgg                                                                          <  1:272552/150‑1 (MQ=255)
                                                                               tACAATGAAATACAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGAc                                                                        <  2:1669744/150‑1 (MQ=255)
                                                                                   aTGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtct                                                                    >  1:1465230/1‑150 (MQ=255)
                                                                                   aTGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtct                                                                    >  2:2625598/1‑150 (MQ=255)
                                                                                    tGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtctc                                                                   >  1:417024/1‑150 (MQ=255)
                                                                                    tGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtctc                                                                   <  1:1584914/150‑1 (MQ=255)
                                                                                     gAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTcc                                                                  >  2:2867071/1‑150 (MQ=255)
                                                                                     gAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTcc                                                                  >  2:2179878/1‑150 (MQ=255)
                                                                                      aaaTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTccc                                                                 <  1:1110354/150‑1 (MQ=255)
                                                                                      aaaTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTccc                                                                 >  1:3422250/1‑150 (MQ=255)
                                                                                            aaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTc                                                           >  2:329798/1‑150 (MQ=255)
                                                                                            aaGGATTTATTTCTGGTCACGTCCACACATAGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCAGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTc                                                           >  2:1511426/1‑150 (MQ=255)
                                                                                              ggATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcac                                                         >  1:1186873/1‑150 (MQ=255)
                                                                                               gATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCACCGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcaca                                                        >  2:1701784/1‑150 (MQ=255)
                                                                                               gATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcaca                                                        <  2:469546/150‑1 (MQ=255)
                                                                                               gATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcaca                                                        >  1:594199/1‑150 (MQ=255)
                                                                                                atttatttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACaa                                                       >  2:1898834/1‑150 (MQ=255)
                                                                                                         tGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACTTCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACATGGACTTCTCCCGGCTTCACAATGTATCGCt                                              >  1:3657260/1‑150 (MQ=255)
                                                                                                           gTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCACGACTGAGGGGCTTCCTGGTTCTCATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTGCTCCCGGCTTCACACTGGATCGCGGt                                            >  2:3487027/1‑150 (MQ=255)
                                                                                                                 tCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGAc                                      >  2:2385034/1‑150 (MQ=255)
                                                                                                                   cacacaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTc                                    >  2:89431/1‑150 (MQ=255)
                                                                                                                    acacaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCa                                   >  2:2234281/1‑150 (MQ=255)
                                                                                                                    acacaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCa                                   >  2:1183951/1‑150 (MQ=255)
                                                                                                                    acacaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCa                                   >  2:1815350/1‑150 (MQ=255)
                                                                                                                          tGACCACATCGACAAAACAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtg                             <  1:3186145/150‑1 (MQ=255)
                                                                                                                           gACCACATCGACAAAATAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCATCACAATGTATCGCTGTACCGACTCATAAGtga                            >  2:3410825/1‑150 (MQ=255)
                                                                                                                           gACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACCTAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACACTGTCTCGCGGTACCGACTCATAAGtga                            >  2:3501769/1‑150 (MQ=255)
                                                                                                                           gACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACATTGTATCTCTGTACCGAATCATAAGtgt                            >  2:17902/1‑149 (MQ=255)
                                                                                                                           gACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAATACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTCCCGACTCCGAAGtga                            >  2:4039429/1‑150 (MQ=255)
                                                                                                                                caTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAAc                       >  1:1347557/1‑150 (MQ=255)
                                                                                                                                caTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAAc                       >  1:1587249/1‑150 (MQ=255)
                                                                                                                                caTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAAc                       >  1:3025627/1‑150 (MQ=255)
                                                                                                                                   cGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTg                    <  1:3968054/150‑1 (MQ=255)
                                                                                                                                    gACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTgg                   <  1:3648352/150‑1 (MQ=255)
                                                                                                                                       aaaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcgc                >  2:358061/1‑150 (MQ=255)
                                                                                                                                       aaaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcgc                >  2:3704312/1‑150 (MQ=255)
                                                                                                                                       aaaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcgc                >  2:4007673/1‑150 (MQ=255)
                                                                                                                                         ggaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACTGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTg              >  2:422201/3‑150 (MQ=255)
                                                                                                                                          aaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGc             <  2:651262/150‑1 (MQ=255)
                                                                                                                                          aaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATAGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGc             >  1:3947507/1‑150 (MQ=255)
                                                                                                                                            aGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCaa           <  2:1398831/150‑1 (MQ=255)
                                                                                                                                               cccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTc        <  1:690343/150‑1 (MQ=255)
                                                                                                                                                ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCa       >  1:2268032/1‑150 (MQ=255)
                                                                                                                                                ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCa       <  2:3639060/150‑1 (MQ=255)
                                                                                                                                                 cTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCAc      >  2:483748/1‑150 (MQ=255)
                                                                                                                                                  tCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACg     >  2:1604055/1‑150 (MQ=255)
                                                                                                                                                    gACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGtt   >  2:2213185/1‑150 (MQ=255)
                                                                                                                                                     aCTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTc  <  1:2625598/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTC  >  NC_000913/2167130‑2167428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: