Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,167,280 C→T 5.4% intergenic (+80/+22) cyaR → / ← ogrK sRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,167,2800CT 5.4% 832.2 / 22.1 241intergenic (+80/+22)cyaR/ogrKsRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth
Reads supporting (aligned to +/- strand):  ref base C (126/102);  new base T (5/8);  total (131/110)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

AATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTCT  >  NC_000913/2167135‑2167429
                                                                                                                                                 |                                                                                                                                                     
aaTGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTTTAATCTCCCTTACACGGGCTTATTTTTTACGCTTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAg                                                                                                                                                   <  1:2159273/150‑1 (MQ=255)
  tGCTAGCTTTACCCGGAACCACCTCCTTCGCGTTTGTAATCTCCCTCACACGGGCTTATTTTTTCCGCTTAATACAATTAAAAAAAAGGATTTTTTTCTGGTCACGTCCAAACATTGACCACATCGACAAAAAAGCCCCTCGACTGAggg                                                                                                                                                 <  1:735468/150‑1 (MQ=255)
     tAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCTTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCt                                                                                                                                              <  1:130424/150‑1 (MQ=255)
     tAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCt                                                                                                                                              >  2:2077873/1‑150 (MQ=255)
       gCTGTCCCCGGAACCACCGCCTTAGCCGGTGTAATCTCCCTTACCCGGGCTTATTTTTTACGCGTAATACAATTAAATAAAAGGATTTAGTTCTGGTCACGTCAACACTTTGACCACATCGACAAACAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                            <  1:3914430/150‑2 (MQ=255)
       gCTGTACCAGGAACCCCCTCCTTAGCCTGTTTAATCTCCCTTACACGGGCTTATTTTTTCCGCGTAATACAATGAAATAAAAGGATTTATTTCTGGACACGCCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                            <  1:1399109/150‑2 (MQ=255)
       gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                                                                                                                                            >  2:3968054/1‑150 (MQ=255)
       gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                            <  2:2396425/150‑2 (MQ=255)
       gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttt                                                                                                                                            <  1:2684406/150‑2 (MQ=255)
         tGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAAAAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCt                                                                                                                                          <  1:1218314/150‑1 (MQ=255)
         tGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTAACGCTTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCt                                                                                                                                          <  1:3556734/150‑1 (MQ=255)
           tACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGt                                                                                                                                        <  1:952196/150‑1 (MQ=255)
           tACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGt                                                                                                                                        >  1:2411413/1‑150 (MQ=255)
             ccAGGCCCCACCTCCTTCGCCTGTGTACGCTCCCGGCCCCGGGCGTATTTTTGCCGCGTAATACCCTGCACTACCAGGATTTATTTCTGGTCACGGCCACACATTGACCACATCGACCAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                      <  2:163230/150‑1 (MQ=255)
             ccAGGACCCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGATTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                      <  1:1039889/150‑1 (MQ=255)
              cAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTc                                                                                                                                     >  1:1910098/1‑150 (MQ=255)
              cAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTc                                                                                                                                     >  1:3037371/1‑150 (MQ=255)
              cAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTc                                                                                                                                     >  1:3959749/1‑150 (MQ=255)
                 gAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGGAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAACAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTaa                                                                                                                                  <  2:3448401/150‑1 (MQ=255)
                  aaCCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAt                                                                                                                                 >  1:3489081/1‑150 (MQ=255)
                   accaccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAtt                                                                                                                                <  1:1138073/150‑1 (MQ=255)
                     caccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTAc                                                                                                                              <  2:83041/150‑1 (MQ=255)
                        ctcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATc                                                                                                                           >  1:1767122/1‑150 (MQ=255)
                            ttAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCcaca                                                                                                                       >  1:2073461/1‑150 (MQ=255)
                                   gtgtAATCTCCCTTACACGGGCTTATTTTTTACGCTTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtg                                                                                                                <  1:3770889/150‑1 (MQ=255)
                                   gtgtAATCTCCCTTACACGGGCTTATTTATTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtg                                                                                                                <  1:1430843/150‑1 (MQ=255)
                                   ctgtAATCGCCCTTCCACGGGCTTATTTTTTACGCGTAAGACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACAGTGACCACATCGACAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAGTACATCCACATAATTtgc                                                                                                               <  2:2382715/149‑1 (MQ=255)
                                    tgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                               <  2:770388/150‑1 (MQ=255)
                                      tAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgctg                                                                                                             <  1:2134112/150‑1 (MQ=255)
                                        aTCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGcc                                                                                                           <  2:3952532/150‑1 (MQ=255)
                                        aTCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGcc                                                                                                           >  2:372721/1‑150 (MQ=255)
                                            cccTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATATAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGa                                                                                                       <  2:1710325/150‑1 (MQ=255)
                                              cTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                     <  2:2685874/150‑1 (MQ=255)
                                              cTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                     >  1:722559/1‑150 (MQ=255)
                                              cTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                     <  2:1829236/150‑1 (MQ=255)
                                               ttACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACgg                                                                                                    <  1:2725761/150‑1 (MQ=255)
                                               ttACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACgg                                                                                                    >  2:3330792/1‑150 (MQ=255)
                                                 acacGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCa                                                                                                  >  2:315089/1‑150 (MQ=255)
                                                   acGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAAc                                                                                                >  2:810820/1‑150 (MQ=255)
                                                     gggCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTGATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACgg                                                                                              <  1:2980619/150‑1 (MQ=255)
                                                       gCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGt                                                                                            <  1:1937318/150‑1 (MQ=255)
                                                          tATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCACCGGGTGCg                                                                                         >  2:4066091/1‑150 (MQ=255)
                                                           aTTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCgg                                                                                        >  2:272552/1‑150 (MQ=255)
                                                            ttttttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGc                                                                                       <  2:2122914/150‑1 (MQ=255)
                                                            ttttttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGc                                                                                       >  1:504790/1‑150 (MQ=255)
                                                            ttttttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGc                                                                                       <  1:1993219/150‑1 (MQ=255)
                                                             tttttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGcc                                                                                      >  2:3993966/1‑150 (MQ=255)
                                                               tttACGCGTAATCCAATGAAATAAAATGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCtt                                                                                    <  2:858702/150‑1 (MQ=255)
                                                                ttACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACCTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTa                                                                                   >  2:3222801/1‑150 (MQ=255)
                                                                   cgcgTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTacga                                                                                <  1:280283/150‑1 (MQ=255)
                                                                      gTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGt                                                                             <  1:1784689/150‑1 (MQ=255)
                                                                       tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                            <  1:3607395/150‑1 (MQ=255)
                                                                       tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                            >  2:1160594/1‑150 (MQ=255)
                                                                       tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                            <  2:277170/150‑1 (MQ=255)
                                                                       tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                            <  1:3022075/150‑1 (MQ=255)
                                                                       tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                            <  1:1769330/150‑1 (MQ=255)
                                                                       tAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTg                                                                            <  1:1970733/150‑1 (MQ=255)
                                                                        aaTACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTgg                                                                           <  1:272552/150‑1 (MQ=255)
                                                                          tACAATGAAATACAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGAc                                                                         <  2:1669744/150‑1 (MQ=255)
                                                                              aTGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtct                                                                     >  1:1465230/1‑150 (MQ=255)
                                                                               tGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTtctc                                                                    <  1:1584914/150‑1 (MQ=255)
                                                                                gAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTcc                                                                   >  2:2867071/1‑150 (MQ=255)
                                                                                 aaaTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTccc                                                                  <  1:1110354/150‑1 (MQ=255)
                                                                                        aGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTca                                                           <  1:3969846/150‑1 (MQ=255)
                                                                                         ggATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcac                                                          >  1:1186873/1‑150 (MQ=255)
                                                                                          gATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCACCGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcaca                                                         >  1:347653/1‑150 (MQ=255)
                                                                                          gATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcaca                                                         <  2:469546/150‑1 (MQ=255)
                                                                                           atttatttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACaa                                                        <  2:2208352/150‑1 (MQ=255)
                                                                                           atttatttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACaa                                                        >  2:1898834/1‑150 (MQ=255)
                                                                                            tttatttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAAt                                                       <  1:3051469/150‑1 (MQ=255)
                                                                                                  tCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCg                                                 <  1:3287649/150‑1 (MQ=255)
                                                                                                    tGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCt                                               <  2:1555043/150‑1 (MQ=255)
                                                                                                      gTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCACGACTGAGGGGCTTCCTGGTTCTCATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTGCTCCCGGCTTCACACTGGATCGCGGt                                             >  2:3487027/1‑150 (MQ=255)
                                                                                                        cACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTAc                                           >  1:3660797/1‑150 (MQ=255)
                                                                                                              cacacaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTc                                     >  2:89431/1‑150 (MQ=255)
                                                                                                               acacaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACCTAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCa                                    >  2:2233606/1‑150 (MQ=255)
                                                                                                                   aTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGTCTTCTCCCGGCTTGTCAATGTATCGGTGTACCGTCTCATAAg                                >  1:3837363/1‑150 (MQ=255)
                                                                                                                    ttGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGt                               >  1:3344349/1‑150 (MQ=255)
                                                                                                                     tGACCACATCGACAAAACAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtg                              <  1:3186145/150‑1 (MQ=255)
                                                                                                                      gACCACATCGACAAAATAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCATCACAATGTATCGCTGTACCGACTCATAAGtga                             >  2:3410825/1‑150 (MQ=255)
                                                                                                                      gACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACCTAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACACTGTCTCGCGGTACCGACTCATAAGtga                             >  2:3501769/1‑150 (MQ=255)
                                                                                                                      gACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACATTGTATCTCTGTACCGAATCATAAGtgt                             >  2:17902/1‑149 (MQ=255)
                                                                                                                      gACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAATACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTCCCGACTCCGAAGtga                             >  2:4039429/1‑150 (MQ=255)
                                                                                                                         cacaTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtgatga                          <  2:1381028/150‑1 (MQ=255)
                                                                                                                          acaTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGaa                         >  2:1253059/1‑150 (MQ=255)
                                                                                                                           caTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAAc                        >  1:1347557/1‑150 (MQ=255)
                                                                                                                           caTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAAc                        >  1:1587249/1‑150 (MQ=255)
                                                                                                                               gACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTgg                    <  1:3648353/150‑1 (MQ=255)
                                                                                                                               gACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTgg                    >  2:1426695/1‑150 (MQ=255)
                                                                                                                               gACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTgg                    >  2:2660611/1‑150 (MQ=255)
                                                                                                                               gACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTgg                    <  2:2419839/150‑1 (MQ=255)
                                                                                                                                  aaaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcgc                 >  2:358061/1‑150 (MQ=255)
                                                                                                                                  aaaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcgc                 >  2:4007673/1‑150 (MQ=255)
                                                                                                                                    ggaaGCCCCTCAGTCGAGGGGCTTTCTGTTTCTAATTAAATACACAAAATTTGCTGCCCTGACGGCAACGGGTGCGGCCATACGACGTGGACTACTCCCGGCTTCAAAATGTATCGCTGTACCGACTCATAAGAGATGAACGTGGCGCTg               >  1:644995/3‑150 (MQ=255)
                                                                                                                                    ggaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACTGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTg               >  2:422201/3‑150 (MQ=255)
                                                                                                                                     aaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGc              <  2:651262/150‑1 (MQ=255)
                                                                                                                                           ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCa        >  1:2268032/1‑150 (MQ=255)
                                                                                                                                           ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCa        <  2:3639060/150‑1 (MQ=255)
                                                                                                                                            cTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCAc       >  2:483748/1‑150 (MQ=255)
                                                                                                                                                aCTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTc   <  1:2625598/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
AATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTCT  >  NC_000913/2167135‑2167429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: