Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 16.6% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA16.6% 362.4 / 12.2 156intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (50/71);  new base A (19/11);  total (74/82)
Fisher's exact test for biased strand distribution p-value = 4.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

CAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177090‑2177383
                                                                                                                                                |                                                                                                                                                     
cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTCAAAat                                                                                                                                                  <  1:1255505/150‑1 (MQ=255)
cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                  <  1:1255435/150‑1 (MQ=255)
cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                  >  2:1681969/1‑150 (MQ=255)
  gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAatat                                                                                                                                                >  2:1434770/1‑150 (MQ=255)
  gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                <  2:133589/150‑1 (MQ=255)
  gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                >  2:891085/1‑150 (MQ=255)
  gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                <  2:1996038/150‑1 (MQ=255)
  gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                <  2:2980857/150‑1 (MQ=255)
  gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAatat                                                                                                                                                >  1:1851346/1‑150 (MQ=255)
  gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAatat                                                                                                                                                >  2:2421341/1‑150 (MQ=255)
   cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAtata                                                                                                                                               >  1:1915505/1‑150 (MQ=255)
    ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                              <  1:2414927/150‑1 (MQ=255)
    ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                              <  1:2414970/150‑1 (MQ=255)
          tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                        >  2:2603845/1‑150 (MQ=255)
           accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGTTTTCAATATATAATgttatt                                                                                                                                       >  2:2717419/1‑145 (MQ=255)
           accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                       >  1:3433592/1‑150 (MQ=255)
            ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                      >  2:590768/1‑150 (MQ=255)
            ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                      <  2:2157778/150‑1 (MQ=255)
            ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAaata                                                                                                                                      >  1:2570964/1‑150 (MQ=255)
             caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAAAGAAattat                                                                                                                                     >  1:239003/1‑150 (MQ=255)
                cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                                  >  2:3425079/1‑150 (MQ=255)
                 acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATTAAAATAttttt                                                                                                                                 >  1:2797567/1‑148 (MQ=255)
                   ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATGAAATTATtttttt                                                                                                                               >  2:2840307/1‑150 (MQ=255)
                     gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAAATATTTGttttt                                                                                                                             >  2:2243552/1‑150 (MQ=255)
                     gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAAATATtttttttt                                                                                                                             >  2:2243990/1‑150 (MQ=255)
                      cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                            <  2:1936441/150‑1 (MQ=255)
                      cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                            <  1:2874545/150‑1 (MQ=255)
                        tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                          <  1:456725/150‑1 (MQ=255)
                           tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAAATATTTTTTTTTAAatat                                                                                                                       >  1:1595333/1‑150 (MQ=255)
                           tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTGTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATTAAAAAATTTTTTTTTAAatat                                                                                                                       >  1:1595325/1‑150 (MQ=255)
                              cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  2:1642815/150‑1 (MQ=255)
                               gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATagag                                                                                                                   >  2:379070/1‑150 (MQ=255)
                                       gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGTAATTATTTTTTTTTAAATATAGAGATAACGAt                                                                                                           >  1:1041830/1‑150 (MQ=255)
                                       gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTAAAATATTTTTTTTTTAATATAGAGATAAAGAt                                                                                                           >  1:3917509/1‑150 (MQ=255)
                                               aaTGGCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  2:2811537/150‑1 (MQ=255)
                                               aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  2:908457/150‑1 (MQ=255)
                                               aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATCACaaaaa                                                                                                   >  1:335496/1‑150 (MQ=255)
                                               aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATATAATGTAATTATTTTTTTTTTAATATCGAGATAACGATCACaaaaa                                                                                                   >  2:8417/1‑150 (MQ=255)
                                                aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                  <  2:786404/150‑1 (MQ=255)
                                                 tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                 >  2:405838/1‑150 (MQ=255)
                                                    cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATTAAATTATTTTTTTTTAAATATAGATATAACGATAACAAAAAAGaaa                                                                                              >  1:859590/1‑148 (MQ=255)
                                                    cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAAATATTTTTTTTTAAATATAGAGATAAACAAAACAAAAACGACa                                                                                              >  1:1689670/1‑150 (MQ=255)
                                                     ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCTTCTGTTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTTTTTTCTTTTTCAAAATATAATTAATTTATTTTTTTTTAAATATCTATATAACTATCACAAAAACGACaa                                                                                             <  2:3995104/150‑1 (MQ=255)
                                                     ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTGAAAAAATAATGTAATTATTTTTTTTTAAATATAGAGTTAAAGGAGACAAAATAGACaa                                                                                             >  1:1143366/1‑150 (MQ=255)
                                                      gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAAATATTTTTTTTTAAAAATCGAGATAAAGATAACAAAAACGACaaa                                                                                            >  1:2141022/1‑149 (MQ=255)
                                                          cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGa                                                                                        <  2:3214784/150‑1 (MQ=255)
                                                           gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATACCAAAAACGACAATATGaa                                                                                       >  1:4077698/1‑150 (MQ=255)
                                                           gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACCATATgta                                                                                       >  1:4077756/1‑148 (MQ=255)
                                                            cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAAAATTaaa                                                                                      >  1:2724529/1‑150 (MQ=255)
                                                               aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatt                                                                                   <  2:1328427/150‑1 (MQ=255)
                                                               aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatt                                                                                   <  2:1328022/150‑1 (MQ=255)
                                                                aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAatta                                                                                  <  2:1890540/150‑1 (MQ=255)
                                                                 ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAAAGATAACAAAAAAGACAAAATGAAAattat                                                                                 >  2:1253906/1‑150 (MQ=255)
                                                                  cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                <  2:3689703/150‑1 (MQ=255)
                                                                        gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTTAATTATTTTTTTTTTAAATTGGAGATAAAGATACCAAAAAAAAAAAAATTAAAATTATTCgatgg                                                                          >  1:3664935/1‑147 (MQ=255)
                                                                               gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                   <  2:4122988/150‑1 (MQ=255)
                                                                                 tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                 <  2:276240/150‑1 (MQ=255)
                                                                                   ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTTAAAATATAAAAAAAATATTTTTTTTTAAATATATAGAAAACAATAAAAAAAAAGACAAAATTGAAAAAATTAGAAGAGTGAAAgtaaa                                                               >  1:2337094/1‑146 (MQ=255)
                                                                                   ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTTAAAAAAAAAATAAAATATTTTTTTTTTAATATATATATAAAGATAACAAAAAACAAAAAAAGAAAATTATTAGTGGAGTGGAATGCaa                                                               >  1:538935/1‑150 (MQ=255)
                                                                                    tccgCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAATTTATTTTTTTTTAAATATCGATATAAGTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  1:2749604/147‑1 (MQ=255)
                                                                                    tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAAAAAAATTAAAATATTTTTTTTTAAATATATAGATAAAGAAAAAAAAAAACAAAAAAATAAAAATATTCTAGGAGGGGAAGGCaaa                                                              >  1:1892881/1‑150 (MQ=255)
                                                                                    tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTTAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              >  2:2261740/1‑150 (MQ=255)
                                                                                            tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      >  2:4101049/1‑150 (MQ=255)
                                                                                                   tctaTACCACTTACATTTTCATCTCCTGTCGTTTGTTTTCGATTTCAAATTATAATGAATTTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:354817/147‑1 (MQ=255)
                                                                                                   tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:2380962/150‑1 (MQ=255)
                                                                                                   tCGATACCACGTACATTTTAATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:2092737/150‑1 (MQ=255)
                                                                                                   gcGATACCACGTTAATTTTCATAGCCTGTCGTTTTTTTGCGATTTCAAAATATATTTAAATTATTTGTTTTTAAATATCGTGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:1803058/149‑1 (MQ=255)
                                                                                                   accaaaCCACTAACATTTACATATCATCACTTTTTTTTAACATTAAAAAATATAATCAAATTATTTTTTTTAAAATATCGACATAACCATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:152494/145‑1 (MQ=255)
                                                                                                     gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                             <  2:3790797/150‑1 (MQ=255)
                                                                                                      acccccggTACTTTTTCATTGCCTGTCGTTTTTTTGGGTTTTAAAATTATAATGAATTTTTTTTTTTTTAAATATCGAGATACCGAACAAAAAAACGACAATATGAAAATTATTCGAGGTGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                            <  1:3630433/143‑1 (MQ=255)
                                                                                                       tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTTAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAACGAAAATATGAAAATTATTAGGGGGGTGGAAGGCAAAAAAACGGCCACCCGATAgg                                           >  2:806252/1‑150 (MQ=255)
                                                                                                        cccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                          <  2:1724715/149‑1 (MQ=255)
                                                                                                          cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGAGGGGGGAAAGGCAAAAAAAAGGCCACCCGCTAggggc                                        >  2:2944128/1‑148 (MQ=255)
                                                                                                           acttACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                       <  2:1028073/147‑1 (MQ=255)
                                                                                                           aCGTACATTTTCATTTCCTTTCGTTTGTTTTCTATTTAAAAATATAATTAATTTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                       <  1:2726401/150‑1 (MQ=255)
                                                                                                           aCGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                       <  2:3096073/150‑1 (MQ=255)
                                                                                                             gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGCAGcc                                     >  2:3005312/1‑150 (MQ=255)
                                                                                                             gTACATTTTCATATCCTGTCGTGTTTTTTGGATTTCAAAATATAATGTAATTATTTTTTTTTAAATATAGATGAAAAGATGACAAAAAAGACAAAATGTAAAATATTAGATGAGTGATAGGGAAAAAAACGGACTCCCGATAGGGAAGcc                                     >  2:3223130/1‑150 (MQ=255)
                                                                                                                cATTTTCATACCCGGTCTTTTGTTTTCGATTACAAAATATAATCAAATTATTTGTTTTTAAATATCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGtt                                  <  1:2418962/150‑2 (MQ=255)
                                                                                                                aaTTTTCATATCCTGTCTTTTTTTTTCTATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:2475058/149‑1 (MQ=255)
                                                                                                                aaTTTACTTATCCTGTCTTTTTTTTTCTATTAAAAAATATAATTAAATTATTTGTTTTTAAATATCGATATAACTAACAAAAACACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:384947/149‑1 (MQ=255)
                                                                                                                 aTTTTCATATCCTGTCTTTTTTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                 <  2:1066609/150‑1 (MQ=255)
                                                                                                                 aTTTTCATATCCTGTCGTTTTTTTTAGATTTGAAAATATAATTTAATTATTTTTTTTTTAATATGGTGTTAAAGTTGACAAAAAAGAGAATATTTAAATTTTTTGTGGTGTGTAAGGGAAAAAAAAGGGCTGCCGATATGGGAGGGGTAg                                 >  2:1622401/1‑150 (MQ=255)
                                                                                                                       atatCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                           <  2:1186861/150‑1 (MQ=255)
                                                                                                                        tatCCTGTCTTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                          <  2:2547723/150‑1 (MQ=255)
                                                                                                                                 ttttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 <  1:2673254/149‑1 (MQ=255)
                                                                                                                                     gtttTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  2:3113776/150‑1 (MQ=255)
                                                                                                                                     gtttTCGATTTCAAAATATAAGGAAATTATTTGTGTTTAACTATCGAGATAACGATCACACAAACGACACTATGAAAATTATTCGAGGAGTGAACGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  2:2073253/150‑1 (MQ=255)
                                                                                                                                     gtttTCGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  1:1894707/150‑1 (MQ=255)
                                                                                                                                       tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           <  1:4101049/150‑1 (MQ=255)
                                                                                                                                       tttCGATTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           <  2:859590/150‑1 (MQ=255)
                                                                                                                                          cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg        >  2:3954123/1‑150 (MQ=255)
                                                                                                                                           ggtttCAAGATATAATGAAATTATTTGTGTGTAAATATCGAGATAACGATCACAGAAACGACACTATGCTAATGATGCGAGGCGTGAAAGGCAAAAATACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGTTGGTATGCCggt       >  1:286146/3‑149 (MQ=255)
                                                                                                                                           gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       >  2:3278171/1‑150 (MQ=255)
                                                                                                                                           gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       >  2:2782683/1‑150 (MQ=255)
                                                                                                                                           gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       >  2:2087921/1‑150 (MQ=255)
                                                                                                                                           gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       >  2:236306/1‑150 (MQ=255)
                                                                                                                                            aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt      >  1:346444/1‑150 (MQ=255)
                                                                                                                                             tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg     >  2:3297091/1‑150 (MQ=255)
                                                                                                                                               tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   >  2:878422/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177090‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: