Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 15.3% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT15.3% 361.4 / 6.5 151intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (60/59);  new base T (15/12);  total (76/75)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

TACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  NC_000913/2177100‑2177393
                                                                                                                                                 |                                                                                                                                                    
tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                                  >  2:2603845/1‑150 (MQ=255)
 accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                                 >  1:3433592/1‑150 (MQ=255)
 accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCAAAAAATAATTAAatt                                                                                                                                                 >  2:1138597/1‑150 (MQ=255)
  ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                                <  2:2158064/150‑1 (MQ=255)
  ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                                <  2:2157778/150‑1 (MQ=255)
   caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAAAGAAattat                                                                                                                                               >  1:239003/1‑150 (MQ=255)
     ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                             <  1:2077082/150‑1 (MQ=255)
       acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATTAAAATAttttt                                                                                                                                           >  1:2797567/1‑148 (MQ=255)
         ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATGAAATTATtttttt                                                                                                                                         >  2:2840307/1‑150 (MQ=255)
          tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                                        <  2:3129274/150‑1 (MQ=255)
           gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAAATATtttttttt                                                                                                                                       >  2:2243990/1‑150 (MQ=255)
           gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATGAAATTATTTGttttt                                                                                                                                       >  2:2030498/1‑150 (MQ=255)
            cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                      <  2:1936441/150‑1 (MQ=255)
            cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                      <  1:2874545/150‑1 (MQ=255)
              tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                                    <  1:456725/150‑1 (MQ=255)
                 tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTGTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATTAAAAAATTTTTTTTTAAatat                                                                                                                                 >  1:1595325/1‑150 (MQ=255)
                    cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                              <  2:1642815/150‑1 (MQ=255)
                          tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                        >  2:225122/1‑150 (MQ=255)
                            tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                      <  1:3975322/150‑1 (MQ=255)
                            tgAATAGTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                      <  2:2983105/150‑1 (MQ=255)
                             gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                     <  2:3777476/150‑1 (MQ=255)
                             gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGTAATTATTTTTTTTTAAATATAGAGATAACGAt                                                                                                                     >  1:1041830/1‑150 (MQ=255)
                             gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTAAAATATTTTTTTTTTAATATAGAGATAAAGAt                                                                                                                     >  1:3917509/1‑150 (MQ=255)
                             gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGAt                                                                                                                     >  1:262357/1‑150 (MQ=255)
                                     aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                             <  1:3685054/150‑1 (MQ=255)
                                     aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                             <  2:908457/150‑1 (MQ=255)
                                     aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATATAATGTAATTATTTTTTTTTTAATATCGAGATAACGATCACaaaaa                                                                                                             >  2:8417/1‑150 (MQ=255)
                                     aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATATTCATATCCTGTCGTGGGGTTTCCAATTAAAAATAAAATGAAATTATTTTTTTTTAGATAACGAGATACACAACACaaaaa                                                                                                             >  2:8379/1‑150 (MQ=255)
                                      aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                            <  2:786404/150‑1 (MQ=255)
                                      aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                            <  2:787125/150‑1 (MQ=255)
                                      aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                            <  2:1869807/150‑1 (MQ=255)
                                          cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATTAAATTATTTTTTTTTAAATATAGATATAACGATAACAAAAAAGaaa                                                                                                        >  1:859590/1‑148 (MQ=255)
                                          cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAAATATTTTTTTTTAAATATAGAGATAAACAAAACAAAAACGACa                                                                                                        >  1:1689670/1‑150 (MQ=255)
                                           gtaaCCGCATAACCGCCGCGCTTTGCTTTTTTCAGAATCTGCTTTGTCTATACCCCGTACTTTTTCATTTCCTGTGTTTTTTTTTCGATTTAAAAATATAATCAAATTATTTTTTTTTAAATATCTTTATAACGATCACAAAAACGACaa                                                                                                       <  2:3995161/148‑1 (MQ=255)
                                           ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCTTCTGTTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTTTTTTCTTTTTCAAAATATAATTAATTTATTTTTTTTTAAATATCTATATAACTATCACAAAAACGACaa                                                                                                       <  2:3995104/150‑1 (MQ=255)
                                           ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTGAAAAAATAATGTAATTATTTTTTTTTAAATATAGAGTTAAAGGAGACAAAATAGACaa                                                                                                       >  1:1143366/1‑150 (MQ=255)
                                                cGCATAACCGCCGCGCTGTGGGTTGTTCAGCATCTGCTTTGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTCAAAATATATTTAAATTTTTTGTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGa                                                                                                  <  2:1412934/150‑1 (MQ=255)
                                                 gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACCATATgta                                                                                                 >  1:4077756/1‑148 (MQ=255)
                                                 gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAAAAATGAAATTATTTGTTTTTTAAAATCGAGATAACGATCAAAAAAAAGACAAAATTaa                                                                                                 >  1:4077735/1‑150 (MQ=255)
                                                     aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatt                                                                                             <  2:1328022/150‑1 (MQ=255)
                                                          ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTAATTTTTTTTAAAAATAGAGATAAACAAAACAAAAAAGACAAAAAGAAAattattag                                                                                        >  1:949883/1‑148 (MQ=255)
                                                              gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTTAATTATTTTTTTTTTAAATTGGAGATAAAGATACCAAAAAAAAAAAAATTAAAATTATTCgatgg                                                                                    >  1:3664935/1‑147 (MQ=255)
                                                                       tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                           <  2:276240/150‑1 (MQ=255)
                                                                       tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                           <  2:276268/150‑1 (MQ=255)
                                                                         ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTTAAAATATAAAAAAAATATTTTTTTTTAAATATATAGAAAACAATAAAAAAAAAGACAAAATTGAAAAAATTAGAAGAGTGAAAgtaaa                                                                         >  1:2337094/1‑146 (MQ=255)
                                                                         ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTTAAAAAAAAAATAAAATATTTTTTTTTTAATATATATATAAAGATAACAAAAAACAAAAAAAGAAAATTATTAGTGGAGTGGAATGCaa                                                                         >  1:538935/1‑150 (MQ=255)
                                                                         ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACCACAATATGAAAAATATTCGAGGAGTGAAAGGCaa                                                                         >  2:3345035/1‑150 (MQ=255)
                                                                          tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAAAAAAATTAAAATATTTTTTTTTAAATATATAGATAAAGAAAAAAAAAAACAAAAAAATAAAAATATTCTAGGAGGGGAAGGCaaa                                                                        >  1:1892881/1‑150 (MQ=255)
                                                                                 cTGCTTTGTCGATACCACCTAAATTTTAATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                                 <  2:2816102/150‑1 (MQ=255)
                                                                                  tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                                >  2:4101049/1‑150 (MQ=255)
                                                                                         tctaTACCACTTACATTTTCATCTCCTGTCGTTTGTTTTCGATTTCAAATTATAATGAATTTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                         <  2:354817/147‑1 (MQ=255)
                                                                                         tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                         <  2:2326129/150‑1 (MQ=255)
                                                                                         gcGATACCACGTTAATTTTCATAGCCTGTCGTTTTTTTGCGATTTCAAAATATATTTAAATTATTTGTTTTTAAATATCGTGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                         <  2:1803058/149‑1 (MQ=255)
                                                                                           gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                       <  2:1292351/150‑1 (MQ=255)
                                                                                           gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                       <  2:3790797/150‑1 (MQ=255)
                                                                                                cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGAGGGGGGAAAGGCAAAAAAAAGGCCACCCGCTAggggc                                                  >  2:2944128/1‑148 (MQ=255)
                                                                                                 acttaatttttCATATCTTGTCGTTTTTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                 <  1:2726296/143‑1 (MQ=255)
                                                                                                 aCGTACATTTTCATTTCCTTTCGTTTGTTTTCTATTTAAAAATATAATTAATTTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                 <  1:2726401/150‑1 (MQ=255)
                                                                                                 aCGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                 <  2:3096073/150‑1 (MQ=255)
                                                                                                   gTACATTTTCATATCCTGTCGTGTTTTTTGGATTTCAAAATATAATGTAATTATTTTTTTTTAAATATAGATGAAAAGATGACAAAAAAGACAAAATGTAAAATATTAGATGAGTGATAGGGAAAAAAACGGACTCCCGATAGGGAAGcc                                               >  2:3223130/1‑150 (MQ=255)
                                                                                                     aCATTTTCATATCCTGTCGTTTGTTTTCGATTTGAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAATATTTAAATTATTCGATGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGt                                             >  2:510717/1‑150 (MQ=255)
                                                                                                      cATTTTCATATCCTGTCTTTTTTTTTCGATTAAAAAATATAATAAAATTTTTTTTTTTTAATTATCGATATAACGACCACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGtt                                            <  1:2453230/150‑2 (MQ=255)
                                                                                                      aaTTTTCATATCCTGTCTTTTTTTTTCTTTTTAAAAATATAATTAATTTTTTTGTTTTTAAATATCGATATAACTAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                            <  1:2796377/149‑1 (MQ=255)
                                                                                                      aaTTTTCATATCCTGTCTTTTTTTTTCTATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                            <  1:2475058/149‑1 (MQ=255)
                                                                                                       aTTTTCATATCCTGTCTTTTTTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                           <  2:1066609/150‑1 (MQ=255)
                                                                                                       aTTTTCATATCCTGTCGTTTTTTTTAGATTTGAAAATATAATTTAATTATTTTTTTTTTAATATGGTGTTAAAGTTGACAAAAAAGAGAATATTTAAATTTTTTGTGGTGTGTAAGGGAAAAAAAAGGGCTGCCGATATGGGAGGGGTAg                                           >  2:1622401/1‑150 (MQ=255)
                                                                                                       aTTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGTGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGTAGCCGTAg                                           >  2:3251608/1‑150 (MQ=255)
                                                                                                           tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                       >  2:261957/1‑150 (MQ=255)
                                                                                                             atatCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                                     <  2:1186861/150‑1 (MQ=255)
                                                                                                              tatCCTGTCTTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                    <  2:2547723/150‑1 (MQ=255)
                                                                                                                       ttttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                           <  1:2673254/149‑1 (MQ=255)
                                                                                                                       gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGttt                           >  1:3603419/1‑150 (MQ=255)
                                                                                                                           tttttCTATTTCAAATTATAATTAATTTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                       <  1:1894702/149‑1 (MQ=255)
                                                                                                                           gtttTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                       <  2:3113776/150‑1 (MQ=255)
                                                                                                                             tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                     <  2:1689670/150‑1 (MQ=255)
                                                                                                                                 ggtttCAAGATATAATGAAATTATTTGTGTGTAAATATCGAGATAACGATCACAGAAACGACACTATGCTAATGATGCGAGGCGTGAAAGGCAAAAATACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGTTGGTATGCCggt                 >  1:286146/3‑149 (MQ=255)
                                                                                                                                 gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                 >  2:3834095/1‑150 (MQ=255)
                                                                                                                                 gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                 >  2:2782683/1‑150 (MQ=255)
                                                                                                                                 gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                 >  2:236306/1‑150 (MQ=255)
                                                                                                                                 gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                 >  2:2087936/1‑150 (MQ=255)
                                                                                                                                 gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                 >  2:2087921/1‑150 (MQ=255)
                                                                                                                                  aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                >  1:346444/1‑150 (MQ=255)
                                                                                                                                   tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               >  2:3297091/1‑150 (MQ=255)
                                                                                                                                    ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc              <  2:239003/150‑1 (MQ=255)
                                                                                                                                     tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg             >  2:878422/1‑150 (MQ=255)
                                                                                                                                     tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg             >  2:877939/1‑150 (MQ=255)
                                                                                                                                     tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg             >  1:1601270/1‑150 (MQ=255)
                                                                                                                                      cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg            >  1:1523862/1‑150 (MQ=255)
                                                                                                                                      cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg            >  2:4068696/1‑150 (MQ=255)
                                                                                                                                      cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg            >  2:3582716/1‑150 (MQ=255)
                                                                                                                                       acaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAATAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt           <  2:1535561/148‑1 (MQ=255)
                                                                                                                                           tataCTGAAATTATTTGTTTTTAAATAGCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa       <  2:3736416/150‑1 (MQ=255)
                                                                                                                                            ataATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  2:2797567/150‑1 (MQ=255)
                                                                                                                                            ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  1:2243552/150‑1 (MQ=255)
                                                                                                                                            ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  1:2030498/150‑1 (MQ=255)
                                                                                                                                            ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  1:2243990/150‑1 (MQ=255)
                                                                                                                                              aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa    >  2:1076032/1‑150 (MQ=255)
                                                                                                                                              aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATACGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa    >  2:1512733/1‑150 (MQ=255)
                                                                                                                                                tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa  >  2:478989/1‑150 (MQ=255)
                                                                                                                                                tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGAAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa  >  2:1551854/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  NC_000913/2177100‑2177393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: