Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,649 C→T 7.0% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,6490CT 7.0% 534.1 / 24.1 189intergenic (+256/+459)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (61/113);  new base T (4/9);  total (67/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

GGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACG  >  NC_000913/2304500‑2304797
                                                                                                                                                     |                                                                                                                                                    
 gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                                                                                                                                                     <  1:1417404/150‑1 (MQ=255)
 gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                                                                                                                                                     <  2:1124297/150‑1 (MQ=255)
 gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                                                                                                                                                     <  1:2714225/150‑1 (MQ=255)
      cacCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                <  2:3558177/150‑1 (MQ=255)
       actgccgtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAg                                                                                                                                               <  2:665783/144‑1 (MQ=21)
        ccGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCTCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                                                                                              <  2:937541/150‑1 (MQ=255)
        ccGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                                                                                              <  2:1323317/150‑1 (MQ=255)
         tgccgtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGc                                                                                                                                             <  2:2329730/146‑1 (MQ=255)
          gccgtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                                                                                                            <  2:451590/147‑1 (MQ=37)
           cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg                                                                                                                                           <  1:707760/150‑1 (MQ=40)
           cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg                                                                                                                                           <  1:707387/150‑1 (MQ=40)
           cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg                                                                                                                                           <  1:2364548/150‑1 (MQ=40)
              tgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa                                                                                                                                        <  1:4060719/150‑1 (MQ=40)
                aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAATGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg                                                                                                                                      >  1:372626/1‑150 (MQ=255)
                aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAATGCGGTTAcg                                                                                                                                      >  2:1500892/1‑150 (MQ=255)
                aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg                                                                                                                                      <  1:880800/150‑1 (MQ=255)
                aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTAACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg                                                                                                                                      >  1:22060/1‑150 (MQ=255)
                 aGTGCGGCACCCCGTAGGTCAGACTAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgc                                                                                                                                     <  2:1372088/150‑1 (MQ=255)
                 aGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgc                                                                                                                                     <  1:3317519/150‑1 (MQ=255)
                  gTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgcc                                                                                                                                    <  2:1671118/150‑1 (MQ=255)
                  gTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCCGATAAGGCGGTTAcgcc                                                                                                                                    <  2:3355629/150‑1 (MQ=255)
                   tGCGGCACCCCGTAGGTCCGACACGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccg                                                                                                                                   <  1:538023/150‑1 (MQ=255)
                   tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccg                                                                                                                                   <  2:1203506/150‑1 (MQ=255)
                   tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccg                                                                                                                                   <  2:2846499/150‑1 (MQ=255)
                   tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccg                                                                                                                                   <  1:640527/150‑1 (MQ=255)
                       gCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATc                                                                                                                               <  2:2553776/150‑1 (MQ=255)
                       gCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATc                                                                                                                               >  1:1998931/1‑150 (MQ=255)
                       gCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATc                                                                                                                               >  1:735233/1‑150 (MQ=255)
                       gCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCTCCCCCCCGTCGGGCGGAAAAGGCGGGGACGCCGCagc                                                                                                                               >  1:1319253/1‑148 (MQ=11)
                        cACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                                                              <  2:3022532/150‑1 (MQ=255)
                          ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                            <  1:679083/150‑1 (MQ=255)
                          ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                            <  1:3823281/150‑1 (MQ=255)
                           cccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGAAGGTCGGATAAGGCGGTTACGCCGCATCCGAc                                                                                                                           >  1:432794/1‑150 (MQ=255)
                           cccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGAc                                                                                                                           >  1:432935/1‑150 (MQ=255)
                            ccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACa                                                                                                                          <  2:1268039/150‑1 (MQ=255)
                             cGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAt                                                                                                                         <  1:1768546/150‑1 (MQ=255)
                             cGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAt                                                                                                                         <  1:1960072/150‑1 (MQ=255)
                             cGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAt                                                                                                                         <  1:1175012/150‑1 (MQ=255)
                              gTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATc                                                                                                                        <  2:3173916/150‑1 (MQ=255)
                              gTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATc                                                                                                                        <  1:3394935/150‑1 (MQ=255)
                              gTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATc                                                                                                                        <  1:3394867/150‑1 (MQ=255)
                                 ggTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTaa                                                                                                                     <  1:3111387/150‑1 (MQ=40)
                                  gTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGACCGAGCCGGTTGACAGAGGAGGAGCTTCCGCTACTGAACGGGCCTACAACCCTGTGAAGATCTCCACCCCCTACGTATCATAAGGCGGTTACGCCGCATCCGACATcgcac                                                                                                                    >  2:506536/1‑146 (MQ=2)
                                   tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                   >  2:2038171/1‑150 (MQ=39)
                                   tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                   <  1:2775668/150‑1 (MQ=39)
                                   tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                   <  2:2428915/150‑1 (MQ=39)
                                   tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                   >  1:2173621/1‑150 (MQ=39)
                                   tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGACGGCTGCGACGCAGGCGCGTCTGATAAGGCCTACGCCGCTGTGGAGTGCTCCACCCCGTGGGTCGGTTGAGGCGGGTACGCCGCAGCCGACGGCTAACg                                                                                                                   >  1:3959597/1‑150 (MQ=14)
                                   tCAGACAAGGCGATCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACATCTAACg                                                                                                                   >  1:2465050/1‑150 (MQ=255)
                                    cAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCCGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                                                                                                  <  2:3480541/150‑1 (MQ=38)
                                     cgACCAGGCGGGCACGCCGCAGCCGACAGCCACCGCCCTAGCCGGTTGGCCGATGCGCGGCTTGGGCCTCTTCTGTGGCCTACTTCGCTGTGACGTGGGCCTCCCGCTAGGTCGGATAAGGCGGTGACGCCGCATCCGACATCTAACGcc                                                                                                                 <  2:3959597/149‑1 (MQ=2)
                                     aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcc                                                                                                                 <  2:3298100/150‑1 (MQ=38)
                                      gACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                                                                                                                                                     >  2:3607848/1‑113 (MQ=37)
                                      gACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGccc                                                                                                                >  2:3488434/1‑150 (MQ=37)
                                      gACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGAAGCGACGCTGGCGCGTCTTATCAGGCCTACACACCAGTGAACTGCTCCACCCCGAACGTCGGATAAGGCGGTAACGCCGCATCCAACATATAAcccac                                                                                                                >  1:292071/1‑146 (MQ=11)
                                         aaGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAg                                                                                                             >  1:1780150/1‑150 (MQ=21)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCg                                                                                                                             >  1:482877/1‑133 (MQ=34)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCg                                                                                                                             >  1:3442305/1‑133 (MQ=34)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCg                                                                                                                             <  2:2735581/133‑1 (MQ=34)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCg                                                                                                                             <  2:483060/133‑1 (MQ=17)
                                           ggCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGcc                                                                                                           >  2:3810807/1‑150 (MQ=34)
                                            gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                                                                                                                                                      <  1:2652586/106‑1 (MQ=35)
                                            gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg                                                                                                                                           >  1:1274050/1‑117 (MQ=35)
                                            gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg                                                                                                                                           <  2:1274003/117‑1 (MQ=35)
                                            gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg                                                                                                                                           >  1:1273802/1‑117 (MQ=35)
                                            gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                          <  2:1070573/150‑1 (MQ=34)
                                            gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                          <  2:66178/150‑1 (MQ=34)
                                            gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGAGGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                          >  1:1033350/1‑150 (MQ=255)
                                               gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACATCTAACGCCCAACCCCGtt                                                                                                       >  1:2840262/1‑150 (MQ=255)
                                                    gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACa                                                                                                                          <  1:2106739/126‑1 (MQ=17)
                                                    gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                                                                                                  <  1:3271350/150‑1 (MQ=32)
                                                    gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCAGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                                                                                                  >  2:2106738/1‑150 (MQ=17)
                                                    gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                                                                                                  >  1:234732/1‑150 (MQ=17)
                                                      cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                <  1:3311607/150‑1 (MQ=32)
                                                      cgcATCCGACATCCAACGCCCGAGCCGGTTGCCAGATGCGACGCTGGCGCGTCGTATCAGGCCAACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAGCGAACGCCCCAGCCGGTTGCCTGag                                                                                                >  1:105654/1‑149 (MQ=11)
                                                         aTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCg                                                                                             <  2:3726733/150‑1 (MQ=32)
                                                            cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                                                                                                                                                   <  2:2355128/93‑1 (MQ=33)
                                                             gACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATaa                                                                                                                                                >  2:4058559/1‑95 (MQ=18)
                                                             gACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATaa                                                                                                                                                <  1:4058559/95‑1 (MQ=18)
                                                              tcATCCCACGCCCGTGCGGGTGGCGTGATGCGACTCTGGCGCGTCGTATCTGGCCTACACGGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCt                                                                                        <  1:1475826/149‑1 (MQ=11)
                                                              aCATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCt                                                                                        <  1:1671260/150‑1 (MQ=32)
                                                              aCATCCAACGCCCGAGCCGGGTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTGACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCt                                                                                        <  2:2060311/150‑1 (MQ=255)
                                                              aCAGCCAACGCCCGAGCCGGTTGCCTGATGCGCCGCTGGCGCGACTTATCAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTCGGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCt                                                                                        <  2:2465050/150‑1 (MQ=11)
                                                                aTCCAACGCCCCAGCCGGTTGCCAGATTCGACGCTGGCGCGTCTTATCAGGCCAACACCGCTGTGAAGTTCTCCACCCCGTAGGTCGGATAAGGCGGTTAAGCCGCAACCGACATAAAACGACCAAGCCGGGTTCCTGAAGCGACGCtcg                                                                                      >  2:799513/1‑148 (MQ=11)
                                                                   caccGCCCGTGCCGGTTGCCAGATGCGACTCGGGCGCGCGTTCTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                   <  2:133380/147‑1 (MQ=11)
                                                                   cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                                                                                                                                                      <  2:2612687/83‑1 (MQ=33)
                                                                   cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCATATCAGGCCTACACCCCAGTGAAGTGCTCCCCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGGTGGCTGATGCGGCGCTGgcgc                                                                                   >  1:197148/1‑150 (MQ=11)
                                                                        cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                              >  2:1079998/1‑150 (MQ=11)
                                                                                                                                            cccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGAc          >  1:763325/1‑150 (MQ=32)
                                                                                                                                            cccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGAc          <  1:3810807/150‑1 (MQ=32)
                                                                                                                                             ccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACa         <  2:61649/150‑1 (MQ=32)
                                                                                                                                             ccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACa         >  1:2040425/1‑150 (MQ=32)
                                                                                                                                             ccGTAGGTCAGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACa         <  1:2106738/150‑1 (MQ=17)
                                                                                                                                               ggagctCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATc       <  2:2820380/145‑1 (MQ=12)
                                                                                                                                               gTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATc       <  2:2126502/150‑1 (MQ=32)
                                                                                                                                               gTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATc       <  1:3541724/150‑1 (MQ=32)
                                                                                                                                               gTAGGTCCGATAAGGCGGTCACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATc       <  1:2598437/150‑1 (MQ=12)
                                                                                                                                                   gTCGGATAAGGCGGTTACGCCGCATCCGACATCTTACTCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc   <  2:2485429/150‑1 (MQ=255)
                                                                                                                                                   gTCAGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc   >  2:3557961/1‑150 (MQ=21)
                                                                                                                                                    tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACAACCAACg  >  1:1819573/1‑150 (MQ=21)
                                                                                                                                                     |                                                                                                                                                    
GGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACG  >  NC_000913/2304500‑2304797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: