Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,532 C→A 8.7% V69L (GTA→TTA)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5320CA 8.7% 491.8 / 4.5 177V69L (GTA→TTA) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (87/67);  new base A (19/4);  total (106/71)
Fisher's exact test for biased strand distribution p-value = 2.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

ATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAA  >  NC_000913/238385‑238678
                                                                                                                                                   |                                                                                                                                                  
atCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTAAAt                                                                                                                                                  >  2:1778175/1‑150 (MQ=255)
atCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAt                                                                                                                                                  >  1:425247/1‑150 (MQ=255)
 tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATg                                                                                                                                                 >  2:1132232/1‑150 (MQ=255)
 tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                                                 >  2:19867/1‑150 (MQ=255)
   cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                               <  1:427644/150‑1 (MQ=255)
   cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                               <  1:15548/150‑1 (MQ=255)
    tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                              >  1:2614366/1‑150 (MQ=255)
        aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                          >  1:2322710/1‑150 (MQ=255)
        aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                          >  1:1426503/1‑150 (MQ=255)
        aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                          >  1:1426705/1‑150 (MQ=255)
         cAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTa                                                                                                                                         <  2:184712/150‑1 (MQ=255)
          aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                                        >  2:2267640/1‑150 (MQ=255)
           aaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                                       >  2:1165637/1‑150 (MQ=255)
           aaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                                       >  2:3231902/1‑150 (MQ=255)
             aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                     <  2:2913975/150‑1 (MQ=255)
               gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                                                   <  2:781161/150‑1 (MQ=255)
                    aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                              <  2:3729936/150‑1 (MQ=255)
                    aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                              >  2:2290686/1‑150 (MQ=255)
                     ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAGAGGAAAACg                                                                                                                             <  1:1247249/150‑1 (MQ=255)
                     ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACg                                                                                                                             >  2:1249779/1‑150 (MQ=255)
                              cTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAc                                                                                                                    >  2:678497/1‑150 (MQ=255)
                               tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGaaaac                                                                                                                   >  2:1398022/1‑148 (MQ=255)
                                  aaccaaaGCTATTATTAAAAATATAAGAGTTAGCTTTATTGTTATGTCTGTCGAGAAATTGAATACCATCCTTTTTTTGATTAACAAAGATTTTATTATCTAAACTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGaa                                                                                                                <  2:2990370/144‑1 (MQ=255)
                                       ttgCTATTAGTAAAAATATAAGAGTTAGCTGTTTTGTTTTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                                           <  2:3334960/148‑1 (MQ=255)
                                       tAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                                           <  2:627474/150‑1 (MQ=255)
                                        aGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCCAAGCACAg                                                                                                          >  2:1903789/1‑150 (MQ=255)
                                          atATTTGTAAAATTATAAGAGTTAGCTTTATTGTTATGTCTTTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                        <  2:3914757/149‑1 (MQ=255)
                                           tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATTATTTTATTATAGTAAGTAAAAATAAATTAATATTTAAAAATGAAAACGACAATAAAACGAAGCACAGAAt                                                                                                       >  1:1143806/1‑150 (MQ=255)
                                               aGTAAAAATATAATAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                                   <  2:862350/150‑1 (MQ=255)
                                               aGTAAAAATATAAGAGTTAGCTGTATTTTTATGTCTGTGGCTAAATTGACTACATTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                                   <  2:1586583/150‑1 (MQ=255)
                                                 tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAAACAACAt                                                                                                 >  1:1673161/1‑150 (MQ=255)
                                                 tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAAACAACAt                                                                                                 >  1:2190588/1‑150 (MQ=255)
                                                 tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTGTTTTTGTGTAAGAATGAATTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAATGAAAACGACATTAAAACGAAGCACAGAATCAACAt                                                                                                 >  1:1050926/1‑150 (MQ=255)
                                                 tAAAAATATAAGAGTTAGCTGTATTGTTATGACAGTGGCGAAATTGACTACCTTCGTGTTTTTTTTAAATAAATATTTTATTATATTAAGTAAAATTAAATGAAAATTAAAAAAGTAAAAAGAAATTAAAAAGAACAACAAAAacaacaa                                                                                                 >  1:2967974/1‑149 (MQ=255)
                                                  aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                                >  2:3354352/1‑150 (MQ=255)
                                                    aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                              >  1:812045/1‑150 (MQ=255)
                                                    aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACAAtct                                                                                              >  1:2886654/1‑150 (MQ=255)
                                                     aaTATATGATTTCGCTTTTTTGTTTTGTCTGGGGCTAATTTGACTACCTTCTTTTTTTTTATTAAGATTGATTTTATTATCGTAATTAAATTTACTTGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                             <  1:992251/150‑1 (MQ=255)
                                                        ttaAGAGTTATGTTTATTTTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                          <  2:879403/149‑1 (MQ=255)
                                                         taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTTAAATTACATTAATATTTATAAAGGAAAAGGACATGAAACCGTAGCACAGAATCAACAGTCTGCAAt                                                                                         >  1:2707086/1‑150 (MQ=255)
                                                         taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAATTAAAAATAAATGAATATTTAAAAATGAAAAAGACAAGAAAACCAAGCACAAAATAAACATTCTCCaaa                                                                                         >  1:1595885/1‑149 (MQ=255)
                                                          aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                                                        >  2:147326/1‑150 (MQ=255)
                                                               ttAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAAATAAATGAATATTTAAAAATGAAAAAGACATGAAACCGACGCACAGAATAAACATTATACAATCAaaaa                                                                                   >  1:3917682/1‑150 (MQ=255)
                                                                 aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTAACGTAAGTAAAATTAAAAGAATATTTAAAAATGAAAACCACATGAAAACCAATCACAGAATCAACAATCTCCAaaaataaaat                                                                                 >  1:1297275/1‑150 (MQ=255)
                                                                    tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                              >  2:2452873/1‑150 (MQ=255)
                                                                      tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATAGTAAGTAAAATTAAATTAATATTTAAAAATGAAAAAGACAATAAACCGAAGCAAACAATAAACAATATCAAAAAATAAAATAttta                                                                            >  1:3143884/1‑149 (MQ=255)
                                                                      tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAAAATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                            >  2:1413024/1‑150 (MQ=255)
                                                                       tttGTTTTGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTTATTAATATTTTTTTTATTATCTTAAGTAAAATTACATGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTcc                                                                           <  1:3894444/149‑1 (MQ=255)
                                                                         tGTTATGTCTGTGGCTAACTTTACTACCTTCTTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                         <  1:2079272/150‑1 (MQ=255)
                                                                         tGTTATGTCTGTGGCTAAATTGACTACCTACGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                         <  2:3586104/150‑1 (MQ=255)
                                                                         tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                         <  2:1214335/150‑1 (MQ=255)
                                                                         tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                         >  2:3857570/1‑150 (MQ=255)
                                                                           ttATGTCTGTGGATAAATTGAATACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTgg                                                                       <  1:580257/150‑1 (MQ=255)
                                                                            tATGTCTGTGGCTAAATTGACTACATTCTTTTTTTTGATTAAGAATGATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                      <  1:1323506/150‑1 (MQ=255)
                                                                                      gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAATCACAGAAACAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                            >  2:1610392/1‑150 (MQ=255)
                                                                                      gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                            >  2:1610432/1‑150 (MQ=255)
                                                                                       ctaatttGACAACCTTCTTTTTTTTGATTAATAATGTTTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatta                                                           <  1:2807899/145‑1 (MQ=255)
                                                                                       ctaaaTTGAATACCTTCTTTTTTTTTATTAAGAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatta                                                           <  1:396081/148‑1 (MQ=255)
                                                                                       cGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatta                                                           >  2:1882645/1‑150 (MQ=255)
                                                                                        gAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                          >  2:508086/1‑150 (MQ=255)
                                                                                         aaaTTGACTACCTTCTTTTTTTTTATTAAGAATGATTTTATTATCTTAATTAAAATTACTTGAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                         <  1:1618580/150‑1 (MQ=255)
                                                                                         aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                         <  2:1373224/150‑1 (MQ=255)
                                                                                           aTTGACTACATTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                                       <  2:556241/150‑1 (MQ=255)
                                                                                                cTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAAtata                                                  >  2:1720685/1‑150 (MQ=255)
                                                                                                   ccTTCGTTTTTTTTATTAACAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                               <  1:4058034/150‑1 (MQ=255)
                                                                                                   ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAATTTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                               <  2:2621011/150‑1 (MQ=255)
                                                                                                     ttCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                             <  2:1093570/150‑1 (MQ=255)
                                                                                                     ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                             >  2:2789002/1‑150 (MQ=255)
                                                                                                     ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                             >  2:1315963/1‑150 (MQ=255)
                                                                                                       cGTTTTTTTTATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                           <  1:1167719/150‑1 (MQ=255)
                                                                                                       cGTTTTTTTGATTAATAATTATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                           <  1:1168099/150‑1 (MQ=255)
                                                                                                       cGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAAAATTTAAAAAGGAAAAAGACATTAAAACGAAGCACACAAAAAAAAATATCCAAAAAAAAAATATTTCCGTGGAGCATTTTATTATTGTATATATAGGttt                                           >  1:2914508/1‑150 (MQ=255)
                                                                                                         ttttttttATTAATATTTATTTTATTATCTTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                         <  1:1903789/150‑1 (MQ=255)
                                                                                                         tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                         <  1:3737061/150‑1 (MQ=255)
                                                                                                         tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                         <  2:1256935/150‑1 (MQ=255)
                                                                                                         tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                         >  2:2561587/1‑150 (MQ=255)
                                                                                                         tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                         >  2:3774616/1‑150 (MQ=255)
                                                                                                             tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                     >  1:2495162/1‑150 (MQ=255)
                                                                                                              ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                    >  1:1009461/1‑150 (MQ=255)
                                                                                                               tGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCgg                                   >  2:1894291/1‑150 (MQ=255)
                                                                                                                    aaGAATGATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                              <  1:96320/150‑1 (MQ=255)
                                                                                                                     aGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaa                             >  2:1100236/1‑150 (MQ=255)
                                                                                                                     aGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaa                             >  2:1100237/1‑150 (MQ=255)
                                                                                                                      gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                            >  2:3006894/1‑150 (MQ=255)
                                                                                                                          gATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                        <  2:2963723/150‑1 (MQ=255)
                                                                                                                                attatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCa                  <  1:1023368/150‑1 (MQ=255)
                                                                                                                                  tttCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                <  2:2505199/148‑1 (MQ=255)
                                                                                                                                     cGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa             >  1:2479304/1‑150 (MQ=255)
                                                                                                                                     cGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa             >  2:395903/1‑150 (MQ=255)
                                                                                                                                        aagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGc          <  1:3682802/150‑1 (MQ=255)
                                                                                                                                          gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg        >  2:2637403/1‑150 (MQ=255)
                                                                                                                                          gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg        >  2:2401186/1‑150 (MQ=255)
                                                                                                                                          gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATGTTATTATTGACTATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg        >  2:2401077/1‑150 (MQ=255)
                                                                                                                                           taaAATTACATGAATATATAAAAAGGAAAACGACATGAAACCTAAGCACAGAATCAACATTCTCCAATCATAAAATATTTACGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATAGAATGCAgg       >  1:3625158/1‑150 (MQ=255)
                                                                                                                                            aaaaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAggg      >  1:3821825/1‑150 (MQ=255)
                                                                                                                                            aaaaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAggg      >  1:751230/1‑150 (MQ=255)
                                                                                                                                              aaTTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaa    <  1:3615881/150‑1 (MQ=255)
                                                                                                                                              aaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaa    <  2:2745573/150‑1 (MQ=255)
                                                                                                                                                ttACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa  >  1:2170381/1‑150 (MQ=255)
                                                                                                                                                ttACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa  >  1:2170400/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAA  >  NC_000913/238385‑238678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: