Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,428,697 A→G 5.7% intergenic (‑71/+24) ubiX ← / ← purF 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase/amidophosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,428,6970AG 5.7% 778.3 / 23.1 228intergenic (‑71/+24)ubiX/purF3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase/amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (102/113);  new base G (8/5);  total (110/118)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 231 total aligned reads displayed.

ATCGCGCAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGAC  >  NC_000913/2428549‑2428846
                                                                                                                                                    |                                                                                                                                                     
aTCGCGCAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAg                                                                                                                                                      >  2:50571/1‑150 (MQ=255)
 tCGCGCAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGa                                                                                                                                                     >  1:2008935/1‑150 (MQ=255)
 tCGCGCAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCAAAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCCAGTCAGGGTGCCAGa                                                                                                                                                     >  1:1452125/1‑150 (MQ=255)
  cgcgCAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGAc                                                                                                                                                    <  2:4015371/150‑1 (MQ=255)
  cgcgCAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGAc                                                                                                                                                    <  2:2579105/150‑1 (MQ=255)
    cgcAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCg                                                                                                                                                  <  2:1600195/150‑1 (MQ=255)
       aGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCa                                                                                                                                               >  1:283804/1‑150 (MQ=255)
       aGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCa                                                                                                                                               >  1:2002437/1‑150 (MQ=255)
         aaCCAGAAAAAAGCGCACGCCAAAAATCGCGCCGCAGGCACATCTGAAGCCAACAATGAGTCGTTTCATAAATAGTTGACATGACAGACTAGCATGCAGACAATGCAGGATTACTGCGCGAGTTGAAAGTCAGCGTGCCAGACCGGCAcc                                                                                                                                             >  2:662225/1‑150 (MQ=255)
          aCCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCGGTCTGGCAccc                                                                                                                                            <  1:1034155/150‑1 (MQ=255)
           ccTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCGGTCTGGCACCCt                                                                                                                                           <  1:491285/150‑1 (MQ=255)
             tGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCg                                                                                                                                                  <  1:585776/141‑1 (MQ=255)
               taataaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGc                                                                                                                                       >  2:690123/1‑150 (MQ=255)
                 ataaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGa                                                                                                                                     >  2:3858212/1‑150 (MQ=255)
                 ataaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGa                                                                                                                                     >  1:3530608/1‑150 (MQ=255)
                  taaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGaa                                                                                                                                    <  2:868228/150‑1 (MQ=255)
                  taaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGaa                                                                                                                                    <  1:4041628/150‑1 (MQ=255)
                   aaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAg                                                                                                                                   <  2:1427367/150‑1 (MQ=255)
                        cACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGcatc                                                                                                                              <  2:3509777/150‑1 (MQ=255)
                        cACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGcatc                                                                                                                              <  1:793672/150‑1 (MQ=255)
                        cACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGcatc                                                                                                                              <  1:742509/150‑1 (MQ=255)
                        cACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGcatc                                                                                                                              <  1:2447630/150‑1 (MQ=255)
                           gCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCatcatc                                                                                                                           >  2:2256125/1‑150 (MQ=255)
                           gCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCatcatc                                                                                                                           <  1:314105/150‑1 (MQ=255)
                              aTAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGGGTTGCAAGTCAGGGTGCCAGACCGGCACCCGCAGCGAAGGCATCATCCtt                                                                                                                        >  1:1328763/1‑150 (MQ=255)
                              aTAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCtt                                                                                                                        <  1:3673511/150‑1 (MQ=255)
                              aTAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCtt                                                                                                                        <  2:1949016/150‑1 (MQ=255)
                              aTAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCtt                                                                                                                        >  1:1191943/1‑150 (MQ=255)
                              aTAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCACAGCGAAGGCATCATCCtg                                                                                                                        >  1:951227/1‑149 (MQ=255)
                               tAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTc                                                                                                                       <  1:3909716/150‑1 (MQ=255)
                                  aTCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGtt                                                                                                                    <  1:2951068/150‑1 (MQ=255)
                                     gcgcCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATg                                                                                                                 <  1:1381226/150‑1 (MQ=255)
                                        ccgcTGGCACCGCTGATGCCTACAATGTGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCAt                                                                                                              >  2:1115170/1‑150 (MQ=255)
                                        ccgcTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCAt                                                                                                              >  2:58205/1‑150 (MQ=255)
                                        ccgcTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCAt                                                                                                              >  2:411624/1‑150 (MQ=255)
                                           cTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTCTGCATTTc                                                                                                           >  2:1828152/1‑150 (MQ=255)
                                             ggCACCGCTGCGGCCTACACGGAGGCGTTTCATAAATCGTTGCCCGGTCTGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCACAGCGAAGGCATCATCCTTCGGTATGCAGTTCga                                                                                                         <  2:1347228/150‑1 (MQ=255)
                                             ggCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCga                                                                                                         <  2:1442793/150‑1 (MQ=255)
                                             ggCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCga                                                                                                         <  2:779170/150‑1 (MQ=255)
                                             ggCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCAGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGGGTTGCAAGTCAGGGTGCCAGACCGGCACCCACAGCGAAGGCATCATCCTTCGTTATGCATTTCga                                                                                                         >  1:3304021/1‑150 (MQ=255)
                                                aCCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGAt                                                                                                      <  1:701235/150‑1 (MQ=255)
                                                        tGCCTACAATGAGTCGTTTCATAAAAAGTTTCCCAGTCAGACATGCAGGCAGACATTTCATGATTTAGGCGGGAGTTGCCAGTCAGGGTGCCAGACCCGCACCCACAGCGAAGGCCTCCTCCTTCGTTATTCATTTCGAGATTTTCCAca                                                                                              >  1:3424418/1‑149 (MQ=255)
                                                              ccaTGAGCCGTGTCAGAAATAGTTGCCCGGTCAGACTTGCAGGCAGACTTTGCCGGATTGCGGCGGGAGTTGCACGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTc                                                                                        <  2:2991463/148‑1 (MQ=255)
                                                              cAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTc                                                                                        <  2:2960343/150‑1 (MQ=255)
                                                                 tGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGa                                                                                     <  1:3836690/150‑1 (MQ=255)
                                                                     tCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGtt                                                                                 >  2:2894379/1‑150 (MQ=255)
                                                                        tttCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCa                                                                              >  1:2156313/1‑150 (MQ=255)
                                                                            aTAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGc                                                                          >  1:3590630/1‑150 (MQ=255)
                                                                             tagaTAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCt                                                                         >  2:272444/4‑150 (MQ=255)
                                                                             tAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACCTCGTTCTGACGTTGCACTGCt                                                                         <  1:2651964/150‑1 (MQ=255)
                                                                               aaTAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCttt                                                                       <  2:1765130/150‑1 (MQ=255)
                                                                                  aGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGc                                                                    <  1:3330481/150‑1 (MQ=255)
                                                                                    ttGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGt                                                                  <  2:2561920/150‑1 (MQ=255)
                                                                                     tGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtc                                                                 <  1:2045205/150‑1 (MQ=255)
                                                                                     tGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtc                                                                 <  1:3623258/150‑1 (MQ=255)
                                                                                      gCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtca                                                                >  1:603967/1‑150 (MQ=255)
                                                                                       cccTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtcat                                                               <  1:648294/150‑1 (MQ=255)
                                                                                        ccTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtcatc                                                              >  1:1856516/1‑150 (MQ=255)
                                                                                          tGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTcatcat                                                            >  1:1364088/1‑150 (MQ=255)
                                                                                          tGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTcatcat                                                            >  1:1653810/1‑150 (MQ=255)
                                                                                          tGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTcatcat                                                            >  1:2938586/1‑150 (MQ=255)
                                                                                           gTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCAtt                                                           <  1:2719235/150‑1 (MQ=255)
                                                                                            tCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTa                                                          <  2:4020652/150‑1 (MQ=255)
                                                                                                  ttGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaac                                                    >  2:1851159/1‑150 (MQ=255)
                                                                                                  ttGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaac                                                    >  2:210173/1‑150 (MQ=255)
                                                                                                    gcaggcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaacgt                                                  >  1:2509243/1‑150 (MQ=255)
                                                                                                         cagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGa                                             <  1:2589904/150‑1 (MQ=255)
                                                                                                         cagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGa                                             <  2:2009050/150‑1 (MQ=255)
                                                                                                          agACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAg                                            >  2:242590/1‑150 (MQ=255)
                                                                                                           gACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAgg                                           >  1:172909/1‑150 (MQ=255)
                                                                                                           gACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAgg                                           <  2:2904950/150‑1 (MQ=255)
                                                                                                               ttGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAAt                                       <  2:643288/150‑1 (MQ=255)
                                                                                                                tGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATc                                      <  2:838428/150‑1 (MQ=255)
                                                                                                                   aGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAg                                   >  1:1135601/1‑150 (MQ=255)
                                                                                                                        ttCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGc                              >  1:1505747/1‑150 (MQ=255)
                                                                                                                         tCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATGGAGGTAGcc                             <  1:2444777/150‑1 (MQ=255)
                                                                                                                         tCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGcc                             >  2:2551938/1‑150 (MQ=255)
                                                                                                                         tCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGcc                             >  2:198646/1‑150 (MQ=255)
                                                                                                                            gcggGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTg                          <  2:1058281/150‑1 (MQ=255)
                                                                                                                            gcggGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTg                          >  1:3724137/1‑150 (MQ=255)
                                                                                                                            gcggGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTg                          >  2:3938641/1‑150 (MQ=255)
                                                                                                                             cggGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGa                         >  2:552714/1‑150 (MQ=255)
                                                                                                                              gggAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGAt                        <  1:2127181/150‑1 (MQ=255)
                                                                                                                              gggAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGAt                        <  1:4114561/150‑1 (MQ=255)
                                                                                                                                 aGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCaa                     <  1:4118144/150‑1 (MQ=255)
                                                                                                                                     gCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATc                 >  2:2857744/1‑150 (MQ=255)
                                                                                                                                      cAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCt                >  2:3159695/1‑150 (MQ=255)
                                                                                                                                       aaGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCtt               <  2:3050511/150‑1 (MQ=255)
                                                                                                                                        aGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt              <  2:2210052/150‑1 (MQ=255)
                                                                                                                                         ctgaGGGTGCCGGTCTGGCACCCTGAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTg             >  1:3009812/4‑150 (MQ=255)
                                                                                                                                          tCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTgg            >  2:166849/1‑150 (MQ=255)
                                                                                                                                           cAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt           <  1:1747010/150‑1 (MQ=255)
                                                                                                                                            aGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTg          >  2:1871984/1‑150 (MQ=255)
                                                                                                                                             gggTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGa         >  1:2298083/1‑150 (MQ=255)
                                                                                                                                              ggTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGAc        <  2:36422/150‑1 (MQ=255)
                                                                                                                                              ggTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGAc        >  2:3772157/1‑150 (MQ=255)
                                                                                                                                              ggTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGAc        <  1:196217/150‑1 (MQ=255)
                                                                                                                                               gTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACg       >  1:1098320/1‑150 (MQ=255)
                                                                                                                                                    aGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGAc  >  2:3564139/1‑150 (MQ=255)
                                                                                                                                                    aGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGGTGCACTGCTTTGGCTTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGAc  <  1:459256/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATCGCGCAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGAC  >  NC_000913/2428549‑2428846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: