BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,817,916	T→G	7.2%	intergenic (‑56/+143)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,916	0	T	G	7.2%	533.8 / 27.3	172	intergenic (‑56/+143)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (78/81); new base G (6/7); total (84/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

GGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817770‑2818065

 gTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAag                                                                                                                                                   <  2:3896204/150‑1 (MQ=255)

        agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                            <  2:3834473‑M1/150‑1 (MQ=255)

          aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGC                                                                                                                                          >  2:113591‑M1/1‑150 (MQ=255)

               ggTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG                                                                                                                                     <  2:2939305‑M1/150‑1 (MQ=255)

                gTGCATCCGGGAGGATTCGCACCTCCGACCGCTCGGTTCGGATCCGAGGACTCTATCCAGCTGAGCTACGGATGCATCGGGAACACTTATGCTACTGCTGATATTGATACCGCTACTAACGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                    <  2:2913927‑M1/150‑1 (MQ=255)

                gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                    >  2:1010808‑M1/1‑150 (MQ=255)

                gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                    >  1:4129080‑M1/1‑150 (MQ=255)

                gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                    >  1:3345394‑M1/1‑150 (MQ=255)

                 tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGG                                                                                                                                   <  2:851475‑M1/150‑1 (MQ=255)

                  gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  <  1:2582052‑M1/150‑1 (MQ=255)

                  gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  <  2:3647079‑M1/150‑1 (MQ=255)

                  gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  <  2:1808783‑M1/150‑1 (MQ=255)

                    aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGG                                                                                                                                <  1:3380128‑M1/150‑1 (MQ=255)

                     tCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGA                                                                                                                               >  2:1765524‑M1/1‑150 (MQ=255)

                          gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAAATTATTCTACTGCTGATATTGATACCGATACAAAAGCCATATCAATTAATAGATGTTGAAACCGGGAGGATaaca                                                                                                                          >  2:2555160‑M1/1‑146 (MQ=255)

                                      cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCt                                                                                                              >  2:60333/1‑150 (MQ=255)

                                        ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                            <  1:1018934/150‑1 (MQ=255)

                                          gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGcc                                                                                                          >  2:764590/1‑150 (MQ=255)

                                                tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                                                                    >  1:2248194/1‑150 (MQ=255)

                                                     tCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGt                                                                                               >  2:252312/1‑150 (MQ=255)

                                                       gTAGCCGTGTACTCTCTCCTGCTGCGATAAGGATTCAGCGGGAATAGTTATTCTAGTGCTTTTTTTGATACATATTCTAAAGCCATATCTTTTACGCGATGGTGCCCCCGGGAGGATTTCTCGCCGTGCGGCTCGCCCTTCGGGCCGTTg                                                                                             <  2:1149587/150‑1 (MQ=9)

                                                       gTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTg                                                                                             <  2:3013956/150‑1 (MQ=255)

                                                            cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                        <  2:2629795/150‑1 (MQ=255)

                                                            cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                        <  1:1005710/150‑1 (MQ=255)

                                                            cGAGGACTCTATCCAGCTGAGCTACGGATTCATCGGGAATACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTATGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                        <  1:722834/150‑1 (MQ=255)

                                                             gAGGACTCGATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATCCTACTGGTTATATTGATACCGCGACGACAGCCATATCTATTATGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAg                                                                                       <  1:2814115/150‑1 (MQ=17)

                                                                 aCTCTATCCCGCTGAGCTACGGAGGCATCGGGACAACTTATTCGACTGCAGATATTGACACCGCCACTAAAGCCATATCAATTAAGAGCTGGAGCATCCGGGAGGATGACTCGCCTTGCGGCTCGCCCTTCGGGCCGGTGCTACAGCAAc                                                                                   <  1:1773623/150‑1 (MQ=17)

                                                                 aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCGACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                                   <  2:3719338/150‑1 (MQ=255)

                                                                  ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACg                                                                                  >  1:3056889/1‑150 (MQ=255)

                                                                   tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTcgaa                                                                                                                         <  2:136782‑M1/110‑5 (MQ=255)

                                                                   tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTcgaa                                                                                                                         >  1:136782‑M1/1‑106 (MQ=255)

                                                                   tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGt                                                                                 >  1:503018/1‑150 (MQ=255)

                                                                   tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGt                                                                                 >  1:1737735/1‑150 (MQ=255)

                                                                   tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATCCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgcgcggttcgtagccgagtcatcaatccagc                                                                                 >  2:2728370‑M1/1‑106 (MQ=255)

                                                                     tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTa                                                                               >  1:1861089/1‑150 (MQ=255)

                                                                       tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTagaacctgcgaccgagccggtagtggccgagtactctctccagctgag                                                                             >  2:2163600‑M1/1‑102 (MQ=255)

                                                                       tCCAGCTGAGCTACGGATGCATCGGGAAAACTAATTGTACTGCTGATATTGAAACCGCTACTAAAGCAATATCAATTAAGAGATGGTGAATCCGGGAGGATTGCTCGCCTGGCGGCTCGCCGGTCTGGCCGTTTCTAAAGCAACGTTatc                                                                             >  2:57429/1‑150 (MQ=17)

                                                                          aGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcatc                                                                          >  1:3092876/1‑150 (MQ=255)

                                                                           gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATcc                                                                         <  1:1540388/150‑1 (MQ=255)

                                                                            cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATccc                                                                        <  2:2998946/150‑1 (MQ=255)

                                                                            cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATccc                                                                        <  1:2100100/150‑1 (MQ=255)

                                                                             tgctcTACGGATGCATCGGTAATTCTTATTGTTCTGCTGATATTTTTTCCGCTTCTAAAGCAATATCTTTTTCTTGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCt                                                                       <  1:2149560/146‑1 (MQ=17)

                                                                               aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCATGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAg                                                                     >  2:3436131/1‑150 (MQ=255)

                                                                                gCTACGGATTCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTAATAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGt                                                                    <  1:1103870/150‑1 (MQ=255)

                                                                                     ggATGCATCGGGAAAACTTGTTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCCCGTTGCGCCGCGCCCTGCGGGCCGATGCTACAGCAAAGTTAACATCCCGAGTGccag                                                               >  2:3574939/1‑148 (MQ=18)

                                                                                         gCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                                                           <  2:147056/150‑1 (MQ=255)

                                                                                           aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTa                                                         >  1:2968913/1‑150 (MQ=255)

                                                                                           aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATAATCCCTAGTGCCTGCGGTTa                                                         >  1:2969055/1‑150 (MQ=255)

                                                                                            tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCATTTAAGAGATGGTGCATCCGGGAGGATTCCTCGCCGTGCGGCTCGCCCGTCGGGCCGGTGCTAAGGCGACGTTATCATCCCTAGTGCCTGCGGttcg                                                        >  2:2677291/1‑148 (MQ=18)

                                                                                            tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                        <  1:1010808/150‑1 (MQ=255)

                                                                                               ggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATACCTAGTGCCTGCGGTTAATTc                                                     >  1:2291997/1‑150 (MQ=255)

                                                                                                gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTcc                                                    >  2:1783880/1‑150 (MQ=255)

                                                                                                gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTCAATTcc                                                    >  1:3058179/1‑150 (MQ=255)

                                                                                                gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTAGCATTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGATAACATCCCAAGAGCCTGCGGTTAATTcc                                                    >  2:3534459/1‑150 (MQ=18)

                                                                                                gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctga                                                                              <  2:1877892‑M1/124‑48 (MQ=255)

                                                                                                 aaaaCTTATTCTCCTGCTTATATTGAGACATCTACTAAATCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg                                                   <  1:3673044/150‑1 (MQ=17)

                                                                                                 aaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTTAGAGATGGTGCCTCCGGGTGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg                                                   <  1:990221/150‑1 (MQ=255)

                                                                                                  aaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc                                                  <  1:3152355/150‑1 (MQ=255)

                                                                                                    aCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGAAACGTTATCATCCCTAGTGACTGCGGTTAATTCCGCTa                                                >  2:2822264/1‑150 (MQ=255)

                                                                                                     tttaTTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcgggga                                               >  1:1707095‑M1/2‑72 (MQ=255)

                                                                                                     cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                                               <  1:404606/150‑1 (MQ=255)

                                                                                                     cTTATTCTACTGCTGATATTGATACCGATACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAATCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                                               <  1:404609/150‑1 (MQ=255)

                                                                                                      ttATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTc                                                                                                                         >  2:1801778/1‑75 (MQ=255)

                                                                                                      ttATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTc                                                                                                                         <  1:1801778/75‑1 (MQ=255)

                                                                                                       tagtCTACTGCTGATATTGAGACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAGTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACct                                             <  1:2513039/147‑1 (MQ=255)

                                                                                                          tCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCACGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCtt                                          <  2:3339026/150‑1 (MQ=255)

                                                                                                           cTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                         >  1:2792403/1‑150 (MQ=255)

                                                                                                           cTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                         <  1:60333/150‑1 (MQ=255)

                                                                                                            tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGGTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCg                                        >  1:521188/1‑150 (MQ=255)

                                                                                                                   tatatTGAGAGCTCTTCTAAAGCCATATCTTTTACTAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCtt                                 <  1:2387431/149‑1 (MQ=17)

                                                                                                                   gATATTGATACCTCTAATAAAGCCATATCTATTAATAGATGGTGCATCCGGGAGGTTTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCtt                                 <  1:3886660/150‑1 (MQ=17)

                                                                                                                    atatTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatg                                >  2:1849206‑M1/1‑57 (MQ=255)

                                                                                                                      atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAt                              <  1:943328/150‑1 (MQ=255)

                                                                                                                        tGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagc                            >  1:1885227‑M1/1‑53 (MQ=255)

                                                                                                                         gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCAGCGGATAATTCCGCTACCTCTTCGATACCTACAGTGc                           >  2:1346596/1‑150 (MQ=255)

                                                                                                                         gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATTGCCGCACGCCCTGCGGGCCGATTCAAAAGCAACGGTATCATCCCTAGTGCCAGCGGTTAATACAGCTACCTCTTCGATACCTACAGTGc                           >  2:1069166/1‑150 (MQ=14)

                                                                                                                         gATACCGCTACTAAAGCCATACCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGc                           >  1:1845663/1‑150 (MQ=255)

                                                                                                                           gagagCTTCTACAGGCATATCTTTTCTGAGATGGTGCCTCCGGGTGTATTAGTCTCATTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTg                         <  1:575187/146‑1 (MQ=17)

                                                                                                                             ccGCTACTCCAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                       <  1:4097487/150‑1 (MQ=255)

                                                                                                                             ccGCTACTATATCCATATTAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                       <  1:2607737/150‑1 (MQ=255)

                                                                                                                             ccGCTACTAAAGCCATATCAATTAAGAGATGGTTCATGCGTGATGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                       <  1:1507329/150‑1 (MQ=17)

                                                                                                                              cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAg                      >  1:2736485/1‑150 (MQ=255)

                                                                                                                                   ctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                 <  1:764590/150‑1 (MQ=255)

                                                                                                                                    taAAGCCTTATCAATTTAGAGTTGGTGCATCCGGGTGGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                <  1:2661022/150‑1 (MQ=17)

                                                                                                                                    taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                <  2:3104850/150‑1 (MQ=255)

                                                                                                                                    taAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  >  2:3146044‑M1/1‑36 (MQ=255)

                                                                                                                                    taAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  >  2:299715‑M1/1‑36 (MQ=255)

                                                                                                                                    taAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  >  2:2189972‑M1/1‑36 (MQ=255)

                                                                                                                                      aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAATATTTCGga              <  2:2248194/150‑1 (MQ=255)

                                                                                                                                      aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga              <  2:2248444/150‑1 (MQ=255)

                                                                                                                                        gCCATATCAATTAAGATATGGTGCATCCGGGAGGATTACTCTCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgaga            <  1:81294/150‑1 (MQ=255)

                                                                                                                                         ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa           >  2:2032487/1‑150 (MQ=255)

                                                                                                                                         ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATAGCGGCAAGCCCTGCTGGCCGATTCGAAAGCAACGGTAACATCCCTATTGCCAGCGGTTAATTCCGCTACCTCTAAGATACCATCAGTGCTGAAGATGTCGgagag           >  2:2032488/1‑149 (MQ=18)

                                                                                                                                         ccATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaa                                         >  2:607732‑M1/1‑36 (MQ=255)

                                                                                                                                         ccATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcgg                                                          >  1:2332072‑M1/1‑36 (MQ=255)

                                                                                                                                         ccATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcgg                                                          <  2:2332072‑M1/103‑68 (MQ=255)

                                                                                                                                             atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg       <  2:4051070/150‑1 (MQ=255)

                                                                                                                                             atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTAGCTAAAGAAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATTTg       >  2:149328/1‑150 (MQ=255)

                                                                                                                                               cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt     <  1:1075510/150‑1 (MQ=255)

                                                                                                                                                  ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCa  <  2:4129080/150‑1 (MQ=255)

GGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817770‑2818065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`