BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,158	T→A	5.5%	intergenic (‑23/+40)	argZ ← / ← argY	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,158	0	T	A	5.5%	611.1 / 10.5	185	intergenic (‑23/+40)	argZ/argY	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (103/68); new base A (5/7); total (110/75)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

TTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTT  >  NC_000913/2818012‑2818305

ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑t                                                                                                                                                    >  2:3611582/1‑150 (MQ=255)

 tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑ta                                                                                                                                                   >  1:1760817/1‑150 (MQ=255)

          tctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACATCTGA‑‑AATT‑‑tataaca                                                                                                                                        >  2:277338/1‑147 (MQ=11)

          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCg                                                                                                                                        >  1:2938153/1‑150 (MQ=255)

          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCg                                                                                                                                        >  2:431444/1‑150 (MQ=255)

          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttt                                                                                                                                                <  1:2700725/144‑2 (MQ=255)

          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttt                                                                                                                                                >  2:2700725/1‑143 (MQ=255)

            ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCGGA‑‑TATT‑‑GATACCGct                                                                                                                                      >  1:1420782/1‑150 (MQ=255)

              cGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGctac                                                                                                                                    >  1:2187219/1‑150 (MQ=18)

              cGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGctac                                                                                                                                    <  2:818197/150‑1 (MQ=18)

                aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGctacta                                                                                                                                  >  1:3190593/1‑150 (MQ=255)

                aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGctacta                                                                                                                                  >  1:3190597/1‑150 (MQ=255)

                  aCCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTaaa                                                                                                                                <  1:3340537/150‑1 (MQ=255)

                   ccgtCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAg                                                                                                                               <  2:3058179/147‑1 (MQ=255)

                    cTTCATTTCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGc                                                                                                                              <  1:1783880/150‑1 (MQ=255)

                    cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGc                                                                                                                              <  2:2096543/150‑1 (MQ=255)

                    cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTT‑‑GATACCGCTACTaaa                                                                                                                                <  2:1518565/150‑1 (MQ=11)

                     ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGATGCATCGGGAAATATATTTAACTGCAGA‑‑TATT‑‑GATACCGCTACTAAAGcc                                                                                                                             >  2:4005559/1‑150 (MQ=11)

                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGcc                                                                                                                             >  1:456179/1‑150 (MQ=255)

                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTCCTAGAGcc                                                                                                                             >  2:921222/1‑150 (MQ=255)

                            cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCaa                                                                                                                      <  1:189695/150‑1 (MQ=255)

                            cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCaa                                                                                                                      <  1:2048888/150‑1 (MQ=255)

                            cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTACTTTACTGCAGA‑‑TATTTAGATACCTCTACTAAACACataac                                                                                                                        >  2:1635816/1‑148 (MQ=11)

                              gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAATTTACTGCTGA‑‑TATT‑‑TATACCGCTACTAAAGCCAAATCAAGt                                                                                                                    >  1:3980069/1‑150 (MQ=255)

                               aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTa                                                                                                                   <  2:2961242/150‑1 (MQ=255)

                                 ggttacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCaa                                                                                                                      <  1:1818811/140‑1 (MQ=21)

                                 ggttacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCaa                                                                                                                      >  2:1818811/6‑145 (MQ=21)

                                  aTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAaga                                                                                                                >  1:276231/1‑150 (MQ=255)

                                    ttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAgaga                                                                                                              >  1:2993171/1‑150 (MQ=255)

                                     tCGGAGAAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGAt                                                                                                             <  2:2968958/150‑1 (MQ=255)

                                        gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACAGCTACTAAAGCCATATCAAGTAAGAGATgtt                                                                                                          >  1:405014/1‑148 (MQ=255)

                                         agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCTGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                                         >  2:1607317/1‑150 (MQ=255)

                                         agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAATCCAAGTCAAGTCAGAGATGGTg                                                                                                         >  2:1959541/1‑150 (MQ=255)

                                          gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTaaa                                                                                                                                >  1:2255637/1‑126 (MQ=40)

                                          gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTaaa                                                                                                                                <  2:2255637/126‑1 (MQ=40)

                                                ggTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTTCTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCgg                                                                                                  <  1:1727019/150‑1 (MQ=255)

                                                  tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGgga                                                                                                >  2:1018533/1‑150 (MQ=40)

                                                      tCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAt                                                                                            >  1:1975638/1‑150 (MQ=40)

                                                         gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGctac                                                                                                                                    <  1:1928330/107‑1 (MQ=255)

                                                         gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGAGGGTGCATCCGGGAGGAttag                                                                                         >  1:2340179/1‑148 (MQ=255)

                                                           gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGaa                                                                                       <  1:1025478/150‑1 (MQ=255)

                                                            tggaTTGGAACCTCCTACCGCTGGGTTGGTCGCCTATTCCTATATCCTGCTGTGCTACGTCTGATTCGGTAATTTTATTGTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAc                                                                                      <  1:10823/149‑1 (MQ=14)

                                                                ttCGACCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGACCCACGGATGCACCGGGAAAATTACTTTACTGCTGA‑‑TTTT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                  <  1:31650/150‑1 (MQ=14)

                                                                ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                  >  2:1201902/1‑150 (MQ=255)

                                                                  cgccccaccGACCGCTCGGTTCGTAGCCGAGTACTCTCTCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCCTATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                <  2:2528879/143‑1 (MQ=255)

                                                                   gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGAc                                                                               >  2:3117446/1‑150 (MQ=255)

                                                                    aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGACCCTCCGAcc                                                                              >  2:1928073/1‑150 (MQ=255)

                                                                    aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGACCCTCCGAcc                                                                              >  2:1565284/1‑150 (MQ=255)

                                                                       cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGTGAACTTTATTTTACTGCTGA‑‑TATT‑‑GATAGCGCTACTAAAGCCATATCAAGTGAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                           >  2:2427605/1‑150 (MQ=255)

                                                                       cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                                 >  1:1252626/1‑82 (MQ=255)

                                                                        tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                          <  1:431444/150‑1 (MQ=255)

                                                                        tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                          >  1:706339/1‑150 (MQ=255)

                                                                        tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                          >  2:299734/1‑150 (MQ=255)

                                                                             gcggtgggTTCTTACCCTCGTACTCTTCCCCGCGGAGCTACGGTTGAATGGGTAAATTTATTGTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                     <  1:1218885/144‑1 (MQ=17)

                                                                                 tCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                                 >  1:1209982/1‑150 (MQ=255)

                                                                                    gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                              >  2:485142/1‑150 (MQ=255)

                                                                                     ttCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAGTTTATATTGATGCGGA‑‑TATA‑‑GATACAGGTACGAAAGCAATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                             >  1:3977790/1‑150 (MQ=18)

                                                                                              gAGTACTCTATCCAGCTGAGCTCCGGATGCATCGGGAAATTTAGTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTCGCCGAGTActctct                                                    >  2:3277167/1‑148 (MQ=255)

                                                                                              gAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                                    >  2:4033459/1‑150 (MQ=255)

                                                                                               aGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                   <  1:3611582/150‑1 (MQ=255)

                                                                                               aGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                   <  1:2812433/150‑1 (MQ=255)

                                                                                                gtcctctATCCAGCTGTGCTACGTATGCATGGGGAAACTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCAACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                                  <  2:3092876/147‑1 (MQ=21)

                                                                                                   ctctATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                  >  2:698459/1‑131 (MQ=255)

                                                                                                    tctATCCAGCTGAGCTACGTATGCATAGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                              <  1:3534459/150‑1 (MQ=255)

                                                                                                    tctATCCAGCTGAGCTACGGATGCATCTGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATAAAGCTACTAAAGCCATATAAAGTAAGAGATGGTGAATACGGGAGGATTCGAACATACGACCGCTCGGTTCGTAGCCGATTAATAAATCAAGCt                                              >  2:1168558/1‑150 (MQ=18)

                                                                                                        tCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCAGAGTACTATATCCAGGAGAGc                                          >  1:1279323/1‑150 (MQ=255)

                                                                                                            gCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                      >  2:1400517/1‑150 (MQ=255)

                                                                                                            gCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                      >  1:844376/1‑150 (MQ=255)

                                                                                                            gCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                      >  1:3086860/1‑150 (MQ=255)

                                                                                                            gCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                      >  2:637877/1‑150 (MQ=255)

                                                                                                            gCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                      <  1:2474870/150‑1 (MQ=255)

                                                                                                             cTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                     >  1:644977/1‑150 (MQ=255)

                                                                                                             cTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                     <  1:57429/150‑1 (MQ=255)

                                                                                                             cTGAGCTACGGATGCATCGGGAAATTTATTTAACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                     >  2:3841024/1‑150 (MQ=255)

                                                                                                              tGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                                    >  2:1246504/1‑150 (MQ=255)

                                                                                                                aGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACTGATg                                  >  1:285319/1‑150 (MQ=255)

                                                                                                                  cTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCa                                >  1:933509/1‑150 (MQ=255)

                                                                                                                  cTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCa                                >  1:3417996/1‑150 (MQ=255)

                                                                                                                            aTCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAact                      >  1:3100270/1‑150 (MQ=255)

                                                                                                                            aTCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAact                      <  1:3574939/150‑1 (MQ=255)

                                                                                                                             tCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACAACCACAGACCCAACCCATCGTAGCCGATTACAAAAACCAGCTGACCTACCGATGCCAAGGGAAactt                     >  1:2026606/1‑150 (MQ=9)

                                                                                                                              gggtaaaaTTATATTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATGGGGAAactta                    <  1:2728370/146‑1 (MQ=255)

                                                                                                                              cGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAactta                    >  1:1604413/1‑150 (MQ=255)

                                                                                                                               gggAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttac                   >  1:3731664/1‑150 (MQ=255)

                                                                                                                               gggAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttac                   <  2:3940207/150‑1 (MQ=255)

                                                                                                                                ggAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttact                  <  1:3436131/150‑1 (MQ=255)

                                                                                                                                ggAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGCGAGGATTCGAACCACCGACCGCTCGGTTCGTCGCCGAGTACTCTCTCCAGCTGAGCTACGGATGCCTCGCGAAacttcct                  >  2:2580364/1‑150 (MQ=25)

                                                                                                                                     tttatttTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttact             >  2:2883110/1‑150 (MQ=255)

                                                                                                                                     tttatttTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttact             >  1:1194537/1‑150 (MQ=255)

                                                                                                                                     tttatttTACTGCAGA‑‑TATT‑‑GATACCGCTACTATAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttact             <  1:1069166/150‑1 (MQ=255)

                                                                                                                                     tttatttTACTGCAGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttact             <  1:3895535/150‑1 (MQ=255)

                                                                                                                                        atttTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCa          >  1:1781884/1‑150 (MQ=255)

                                                                                                                                        atttTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCa          >  1:1781851/1‑150 (MQ=255)

                                                                                                                                        atttTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCa          >  1:1367205/1‑150 (MQ=255)

                                                                                                                                        attgTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCa          <  1:3048030/150‑1 (MQ=255)

                                                                                                                                         ttttACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAACGGGAATCTTACCTGACTGCAg         >  2:3505511/1‑150 (MQ=255)

                                                                                                                                               ttctgA‑‑TATT‑‑GATACCGCTACTTTAGCCATATCTAGTATTAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAttttt   <  2:3407528/148‑1 (MQ=18)

                                                                                                                                               tgctgA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                            >  2:1481211/1‑125 (MQ=255)

                                                                                                                                               tgctgA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                            <  1:1481211/125‑1 (MQ=255)

                                                                                                                                                gctgA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAtttttt  <  1:2163600/150‑1 (MQ=255)

TTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATT‑‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTT  >  NC_000913/2818012‑2818305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`