Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,050 T→G 9.9% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,0500TG 9.9% 468.5 / 9.6 207intergenic (‑149/+118)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base T (72/110);  new base G (18/6);  total (90/117)
Fisher's exact test for biased strand distribution p-value = 1.67e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGC  >  NC_000913/3045903‑3046199
                                                                                                                                                   |                                                                                                                                                     
tGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTc                                                                                                                                                     <  2:4016051/150‑1 (MQ=255)
  tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTccc                                                                                                                                                   <  1:2561582/150‑1 (MQ=255)
  tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTccc                                                                                                                                                   >  2:2241797/1‑150 (MQ=255)
  tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTccc                                                                                                                                                   >  2:3190580/1‑150 (MQ=255)
   ttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTcccc                                                                                                                                                  >  2:532686/1‑150 (MQ=255)
     ttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTc                                                                                                                                                <  2:2620022/150‑1 (MQ=255)
      tCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCa                                                                                                                                               >  1:1243421/1‑150 (MQ=255)
        cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAcc                                                                                                                                             <  2:769647/150‑1 (MQ=255)
        cGAGAAAAAGTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTCAAGATTCGCGCCCGGGCCGGCCTGGTGCCCTcccc                                                                                                                                             >  1:825888/1‑150 (MQ=255)
          agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCAcccg                                                                                                                                           >  2:1917989/1‑149 (MQ=255)
            aaaaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTaa                                                                                                                                         <  2:3967019/150‑1 (MQ=255)
             aaaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAc                                                                                                                                        >  2:3059181/1‑150 (MQ=255)
                 ttaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCACACCCGAAaccac                                                                                                                                    >  1:2993637/1‑148 (MQ=255)
                  taattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACACCCAAAcccaca                                                                                                                                   >  2:3516849/1‑147 (MQ=255)
                    attCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCTAACCCACTcc                                                                                                                                 >  1:2439546/1‑150 (MQ=255)
                       cGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCGCCa                                                                                                                              <  1:532777/150‑1 (MQ=255)
                        gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCAAACCCACAACCCaa                                                                                                                             >  1:1916397/1‑150 (MQ=255)
                            aCGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAAACCACTCCCCACAggg                                                                                                                         >  1:524652/1‑150 (MQ=255)
                            aCGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                         >  1:443223/1‑150 (MQ=255)
                                aTCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATAGTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  2:1127073/150‑1 (MQ=255)
                                 tCTTTGAGATTGCTAACAAACTGGCTCAATCTTCTATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                    <  2:3004430/150‑1 (MQ=255)
                                   tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                  <  2:886557/150‑1 (MQ=255)
                                   tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                  <  2:1922632/150‑1 (MQ=255)
                                   tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCGAAACCAAAACCGCACCCCAAAGGGGCGAggg                                                                                                                  >  2:1868499/1‑150 (MQ=255)
                                       agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACt                                                                                                              >  1:3452279/1‑150 (MQ=255)
                                           tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGGGTGTTGCCCTCACCCTAAACCTCTACCCAAAGGGGGGTGGGGTCTGtgt                                                                                                          >  1:605757/1‑148 (MQ=255)
                                           tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGACCCCAAACCCAAAACCCAAACGGGCGAGGGGGCTGTcg                                                                                                          >  1:2805637/1‑149 (MQ=255)
                                           tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCt                                                                                                          <  2:1448372/150‑1 (MQ=255)
                                           tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCACACCCCAAAGGGGCGAGGGGACTGTCt                                                                                                          >  1:1391996/1‑150 (MQ=255)
                                             cTAACAACCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCGCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                        <  1:2274003/150‑1 (MQ=255)
                                             cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                        <  1:3238881/150‑1 (MQ=255)
                                              tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACAACCAAAACCACACCCCAAAGGGGCGAGGGGACAGGCTGAg                                                                                                       >  2:1486488/1‑150 (MQ=255)
                                               aaCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAAACCTCACCCCAAAGGGGCGAGGGGACTGTCTGAGc                                                                                                      >  1:3299607/1‑150 (MQ=255)
                                                  aaaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACTACCCAAAGGGGCGAGGGGACTGGCTGAGCACt                                                                                                   >  1:2012455/1‑150 (MQ=255)
                                                     cTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCCCACCCAAACCCACACCCCAAAGGGGCGCGGGGGCTGTGTGTGCACgttt                                                                                                >  1:2896576/1‑150 (MQ=255)
                                                      tGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAACCACACCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTg                                                                                               >  1:2748073/1‑150 (MQ=255)
                                                      tGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAAACCACACCCCAAAGGGGCGAGGGGACTGTCAGAGCACatttt                                                                                               >  1:2747961/1‑149 (MQ=255)
                                                      tGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCAAAACCACACCCCCAAGGGGCGCGGGGCCAGTCTGAGcccagttg                                                                                               >  1:3941599/1‑143 (MQ=255)
                                                        gCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTa                                                                                             >  2:2548297/1‑150 (MQ=255)
                                                        gCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCCCTTTTGTa                                                                                             >  2:468072/1‑150 (MQ=255)
                                                         cTCAATCTTCTATGTTGGCACGCTTTTCCCCCTCGCCCTGTCAGGGAGAGGGCCGGGGTGAGGTTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTAc                                                                                            <  1:1110822/150‑1 (MQ=255)
                                                         cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTAc                                                                                            <  1:3707587/150‑1 (MQ=255)
                                                          tCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCCCCCCCGAAACCACAACCCAAAAGGGCGGGGGGGCGGGCTGTGGACGTTTGtgat                                                                                           >  2:1760847/1‑147 (MQ=255)
                                                           cAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCGGAGCACTTTTGTACtt                                                                                          <  2:3786712/150‑1 (MQ=255)
                                                            aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                         >  2:2356062/1‑150 (MQ=255)
                                                            aaTCGTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACACCCAAACCCACAACCCAAACGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                         >  1:2231006/1‑150 (MQ=255)
                                                                ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAAACCAATCCCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACTTTGTCa                                                                                     >  2:1792576/1‑150 (MQ=37)
                                                                ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCACTCCCCAAACGGGCGCGGGGACGGTCTGAGCACTTTTTTAAGTTGTCa                                                                                     >  1:2409515/1‑150 (MQ=255)
                                                                  cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATc                                                                                   <  1:530962/150‑1 (MQ=255)
                                                                  cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATc                                                                                   <  1:530406/150‑1 (MQ=255)
                                                                  cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAACGGGCGAGGGGACGGTCTGAGCACGTTTGTACTTTGTCATc                                                                                   >  1:3019560/1‑150 (MQ=255)
                                                                   aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCGAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                  <  2:366831/150‑1 (MQ=255)
                                                                   aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                  <  2:4128614/150‑1 (MQ=255)
                                                                      gTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGAc                                                                               <  1:888415/150‑1 (MQ=255)
                                                                      gTTGGCACGCTTGTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGAc                                                                               <  2:2914099/150‑1 (MQ=255)
                                                                       ttGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCGAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACGTTGTCAACTGACt                                                                              >  2:789128/1‑150 (MQ=255)
                                                                       ttGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCGAACCCTCACCCCCAAGGGGCGGGGGGACTGTCTGAGCACGTTTGTACTTTGTCATCTGACt                                                                              >  1:3002359/1‑150 (MQ=255)
                                                                       ttGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCCCACCCGAACCCACACCCCCAAAGGGGGGGGGGGCGGTCTGAGCACATTTTTTCGTTGTCaaatgtca                                                                              >  2:705947/1‑143 (MQ=255)
                                                                           cACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGTGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCAAACCCAAAACCGCAACCCAAAGGGGCGAGGGGCCTGTCAGTTCACTTTTGTACATTTTCATCTGACTaaaa                                                                          >  2:191639/1‑150 (MQ=255)
                                                                                  ttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACATTTGTACTTTGTCATCTGACAAAAAAGGCGcc                                                                   >  2:3781234/1‑150 (MQ=255)
                                                                                     ccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGaa                                                                <  1:2696667/150‑1 (MQ=255)
                                                                                     ccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGaa                                                                <  1:353985/150‑1 (MQ=255)
                                                                                     ccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGaa                                                                <  2:4074353/150‑1 (MQ=255)
                                                                                     ccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGaa                                                                <  1:1768533/150‑1 (MQ=255)
                                                                                     ccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGaa                                                                <  1:1174600/150‑1 (MQ=255)
                                                                                        cTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTTCTGTGTCATCTGACTAAAAAGGCGCCGAAgcg                                                             >  2:3937516/1‑150 (MQ=255)
                                                                                             ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttt                                                        <  2:1558615/150‑1 (MQ=255)
                                                                                             ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAAACCACTACCCAAAGGGGCGAGGGGACTGTCTGAGCCCTTTTGTACTTTTTCAACTGACTAAAAAGGCGCCGAAGCGCCgtt                                                        >  1:2972251/1‑150 (MQ=37)
                                                                                               tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAg                                                      <  1:2867577/150‑1 (MQ=255)
                                                                                               tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCCCACCCAAACCCACAACCCCAACGGGCGCGGGGCCAGTCTGAGCACAGTTGTACATTTTAAAATGACAAAAAAAGCGCCGAAGCCCCATTAg                                                      >  1:3613619/1‑150 (MQ=255)
                                                                                                ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGa                                                     <  2:782070/150‑1 (MQ=255)
                                                                                                ttCAGGGAGAGGGCCGGGGTGAGGGTAAAGATTCGCGCCAGTGCCGGCCTGCTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttttta                                                     <  2:1637689/150‑4 (MQ=25)
                                                                                                 tCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaa                                                    <  1:3073818/150‑1 (MQ=255)
                                                                                                  cAGGGAGAGGGCCGGGGTGAGGGTAAATCTTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaa                                                   <  1:196039/150‑1 (MQ=255)
                                                                                                  cAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCACTCCCCAAAGGGGCGCGGGGGCTGTCGGAGCCCTTTTGTACTTTGGCATCGGACTAAAAATGCGCCGCAGCGCCATTATaaa                                                   >  2:3830014/1‑150 (MQ=255)
                                                                                                   tgggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                  <  1:3643012/149‑1 (MQ=255)
                                                                                                     ggAGAGGACCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                <  1:1291515/150‑1 (MQ=255)
                                                                                                        gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAATGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                             <  1:1868499/150‑1 (MQ=255)
                                                                                                        gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                             <  1:2933147/150‑1 (MQ=255)
                                                                                                        gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                             <  1:3787727/150‑1 (MQ=255)
                                                                                                        gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                             <  2:3093289/150‑1 (MQ=255)
                                                                                                        gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGGGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                             <  2:3142964/150‑1 (MQ=255)
                                                                                                            gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAAt                                         <  1:749098/150‑1 (MQ=255)
                                                                                                             ccGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTGTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTGTAGAAAATAGTCGAATc                                        >  2:638616/1‑150 (MQ=255)
                                                                                                                     aGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAAtt                                <  2:3331433/150‑1 (MQ=255)
                                                                                                                        gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                             <  1:1226589/150‑1 (MQ=255)
                                                                                                                        gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAACCAAACCCTCTACCCCAAGGGGCGCGGGGACTGTCTGAGCACGTTTGTACTTTGTCAACTGACTAAAAAGGCGCCCAAGCGCCGGTAGAAAATAGTCGAATCAGTGCATTACt                             >  2:1503063/1‑150 (MQ=255)
                                                                                                                           aaTATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGt                          <  2:3473619/150‑1 (MQ=255)
                                                                                                                               ttCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACATTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTc                      <  2:605374/150‑1 (MQ=255)
                                                                                                                                tCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGCCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGCCGAATCAGTGAATTACTGGTATTCg                     <  2:2993637/150‑1 (MQ=255)
                                                                                                                                  gcgcCAGTGCCGGCCTGTTCCCCTCAACCAAACCATCTCACCCAACTGGCGAGGGGACTGTCAGAGACCGTTTGTACTTTTTCAAAATACTAAAAAGGCCCCGAAGCCCCTTTAGAAAATAGTCGAATAATTGAAATAATTGTATTCGCt                   >  2:1182321/1‑150 (MQ=255)
                                                                                                                                   cgcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTa                  <  2:2771806/150‑1 (MQ=255)
                                                                                                                                   cgcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACACCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACAAAAAAGGCGCCGAAGCGCCTTTAGAAAAAAGTCGAATCAGTGAATTAATGGTATTCGcaa                  >  2:3393822/1‑148 (MQ=39)
                                                                                                                                      cAGTGCCGGCCTGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc               <  2:2081814/150‑1 (MQ=255)
                                                                                                                                      cAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc               <  2:2928576/150‑1 (MQ=255)
                                                                                                                                       aGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAACATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCg              <  2:2584633/150‑1 (MQ=255)
                                                                                                                                        gTGCCGGCCTGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCATTAGAAAATAGTCGAATCAGTGAATTACAGGTATTCGCTAATAgg             >  2:3023516/1‑150 (MQ=255)
                                                                                                                                          gCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTa           <  1:163681/150‑1 (MQ=255)
                                                                                                                                             ggCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGc        <  1:3346049/150‑1 (MQ=255)
                                                                                                                                              gCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCa       <  2:3246394/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGC  >  NC_000913/3045903‑3046199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: