Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,678 T→A 7.3% intergenic (+133/‑266) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6780TA 7.3% 575.4 / 2.2 193intergenic (+133/‑266)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (93/76);  new base A (20/2);  total (115/78)
Fisher's exact test for biased strand distribution p-value = 9.61e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

AGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGC  >  NC_000913/3239532‑3239827
                                                                                                                                                  |                                                                                                                                                     
    gggTGGATAATTTTTAATCTGCCTAAGCCGAGTACCCTGTCAATAACATTAGCACAGTTTTCTACCACTACCATCCAGGGAGATGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTTGCCTGTTCCCCACAACCAAACCCACACCCCa                                                                                                                                                >  1:702396/1‑150 (MQ=255)
      gTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                              >  1:3358766/1‑150 (MQ=255)
         gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                                                                                           >  1:1550124/1‑150 (MQ=255)
                tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                                                                                                    >  2:3400539/1‑150 (MQ=255)
                   aaTCTGCCTACGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCGCCCTCCCCCTTCCCGGGTGTGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGa                                                                                                                                 <  2:4016486/150‑1 (MQ=37)
                    aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACTCCCCAAACGGGGCGAGGGGAc                                                                                                                                >  1:1882273/1‑150 (MQ=255)
                      gtGCCTACGCGGTGCACCCTTAAATTAACTTGACCACGGTTTTCGCCCTCCCCCTTCCGGGGTGTGGGTGGGGGGGTGGGTCATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTg                                                                                                                              <  1:616484/149‑1 (MQ=18)
                      cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTg                                                                                                                              >  2:3006876/1‑150 (MQ=255)
                         ccTACGCGGTGTACCGTGTCATTAACTTGCCCCCGGTTTGCCCCCCCCCCCTCCCGGGGCGGGGGTGGGGGTGGGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                                                                                           <  2:1918048/150‑1 (MQ=18)
                           tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                                                                                         >  2:2732163/1‑150 (MQ=255)
                           tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                                                                                         <  2:2709418/150‑1 (MQ=255)
                            aaGCCGTGTACCCTGTCATTAACATGTGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGTGGGTAATTTTTCGCACCGTTGCTGGCCTGTTCCCCTCAGCCTAACCCTCTCCCCAAACGGGGCGAGGGGAGTGTCCGAg                                                                                                                        >  2:1851176/1‑150 (MQ=255)
                            aaGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAg                                                                                                                        >  2:3896464/1‑150 (MQ=255)
                            aaGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCAAACCCTCACCCCAAACGGGGCGAGGGGCCTGACCGAg                                                                                                                        >  2:1850958/1‑150 (MQ=255)
                             aGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGt                                                                                                                       <  2:556973/150‑1 (MQ=255)
                                   ttccccTCTCTTTAAATTGAGCCCGGTTTTCCCCGTCGCCCTCCCCGGTGGTGGGGGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttt                                                                                                                 <  2:2907188/147‑1 (MQ=18)
                                    tACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttttt                                                                                                                >  1:2488895/1‑150 (MQ=255)
                                     aCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCTGGGTGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                                                                                               <  1:3098732/150‑1 (MQ=255)
                                         tGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtt                                                                                                           >  1:3599113/1‑149 (MQ=255)
                                         tGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                                                                                           >  2:1740552/1‑150 (MQ=255)
                                         tGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                                                                                           >  2:1741024/1‑150 (MQ=255)
                                          gTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgt                                                                                                          >  1:238603/1‑150 (MQ=255)
                                               tAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGCGGGGACTGACCGAGTCCTTTTTTGATGTTGtca                                                                                                     >  2:889344/1‑150 (MQ=255)
                                                  cATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatca                                                                                                  <  1:3014993/150‑1 (MQ=255)
                                                   aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                                                                                                 >  2:2356221/1‑150 (MQ=255)
                                                      cgCACCGTTTTCTCCCTCCCCCTTCCCGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCt                                                                                              <  1:1955092/149‑1 (MQ=255)
                                                      aGCACCTTTTTCTCCCTCTCCCTTCCCGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCt                                                                                              <  1:4130591/150‑1 (MQ=37)
                                                      aGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCt                                                                                              >  1:2199308/1‑150 (MQ=255)
                                                      aGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCAAACCCACACCCCAAACCGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCt                                                                                              >  1:3462851/1‑150 (MQ=255)
                                                      aGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCACACCCGAACCCACTCCCCAAACGGGGCGAGGGGACTGACCCAGTCCTTTTTTGATGGTGTCATCAGTCt                                                                                              >  1:6323/1‑150 (MQ=255)
                                                       gccgcGTTTTCTCCCTCGCCCTGCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTg                                                                                             <  2:3740183/146‑1 (MQ=255)
                                                          ccGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGaa                                                                                          >  1:1029439/1‑150 (MQ=255)
                                                               ccgaccctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGc                                                                                     >  2:2430769/5‑150 (MQ=255)
                                                                  tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTGTTTGATGTTGTCATCAGTCTGGAAGCCGCACg                                                                                  >  2:2891035/1‑150 (MQ=255)
                                                                  tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGACCTGTTTTGAAGTTGTCATCAGTCTGGAAGCCGCACg                                                                                  >  2:1114483/1‑150 (MQ=255)
                                                                    cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt                                                                                >  2:2295139/1‑150 (MQ=255)
                                                                    cccctccctTCCCGGGAGTGGGTGGGGGTTTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt                                                                                <  2:1878598/147‑1 (MQ=37)
                                                                     ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg                                                                               >  2:3261725/1‑150 (MQ=255)
                                                                     ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg                                                                               >  2:3330349/1‑150 (MQ=255)
                                                                        tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGcg                                                                            >  2:1242419/1‑149 (MQ=255)
                                                                        tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCTAACCCTATCCCCAAACGGGGCGAGGGGGCGGACCGAGTCCTTTTTTGATGTTGTCATCAGTCGGGAATCCGCACGATGGCt                                                                            >  1:927861/1‑150 (MQ=255)
                                                                           ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTa                                                                         >  1:3222616/1‑150 (MQ=255)
                                                                           ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTa                                                                         >  1:1156630/1‑150 (MQ=255)
                                                                           ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTa                                                                         >  1:3885925/1‑150 (MQ=255)
                                                                              ccAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAttt                                                                      >  1:3843693/1‑150 (MQ=255)
                                                                              ccAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGGTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAttt                                                                      >  1:447286/1‑150 (MQ=255)
                                                                              ccAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAttt                                                                      >  1:2092963/1‑150 (MQ=255)
                                                                               cAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAtttt                                                                     >  2:1630229/1‑150 (MQ=255)
                                                                                aGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAttttt                                                                    >  1:2211946/1‑150 (MQ=255)
                                                                                 ggttgagGGTCGGGTTTTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGTGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                                                                   <  2:2799628/146‑1 (MQ=38)
                                                                                 gggttagGGTGGGGGTGCGGGTCATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                                                                   <  1:1630826/145‑1 (MQ=255)
                                                                                 gggAGAGGGTGGGGGGGTGGGCAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                                                                   <  1:2513276/150‑1 (MQ=255)
                                                                                  gtggtgggTGGGGGGGTGGCTAATTTGTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTAt                                                                  <  2:3464335/145‑1 (MQ=38)
                                                                                  ggtgagGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTAt                                                                  <  2:3978501/147‑1 (MQ=255)
                                                                                    agagGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGt                                                                >  1:3035845/1‑150 (MQ=255)
                                                                                       gggTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCaa                                                             <  2:3185888/150‑1 (MQ=255)
                                                                                        ggTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAACCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTTTCATCAGTATGGAAGCCCCACCTTGGCTTTATTTTTATGTaaaa                                                            >  1:305712/1‑150 (MQ=37)
                                                                                          tCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTAACCCAAACCCACACCCCAAACGGGGCGAGGGGACAGACCGAGTCCATTTTTGATTTTGTCATCAGTATGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGa                                                          >  1:1041534/1‑150 (MQ=255)
                                                                                            ggggTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGaaa                                                        <  1:1805207/150‑1 (MQ=255)
                                                                                            ggggTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGaaa                                                        <  2:2395114/150‑1 (MQ=255)
                                                                                             gggTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAAt                                                       <  1:2745864/150‑1 (MQ=255)
                                                                                               gTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGt                                                     >  2:354311/1‑150 (MQ=255)
                                                                                               gTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACTCCCCAAACGGGGCGAGGGGGCTGACCGGGTCCTTTTTTGTTGTTGTGATAAGGCTGGAAGCCGCCCCGTGGCGTTATTTTTATTTAAAATAAATGt                                                     >  1:1092341/1‑150 (MQ=255)
                                                                                               gTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCGTTATTTTTATGTCAAAGAAATGt                                                     >  1:403797/1‑150 (MQ=255)
                                                                                               gTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGt                                                     >  1:3773982/1‑150 (MQ=255)
                                                                                                  cggttAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAAc                                                  <  1:2015693/146‑1 (MQ=255)
                                                                                                       aaTTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTa                                             <  1:2847553/150‑1 (MQ=255)
                                                                                                         tttttCGCACCGATGCTGGCCTGTTCCCCACACCCTAAACCACTACCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATTTTTTCATCAGTATGGAAGCCGCACGTTTGCTTTATTTTTATGTCAAAGAAATTTAACCAATAAg                                           >  1:4066673/1‑150 (MQ=255)
                                                                                                          ttttCGCACCGATGCTGGCCTGTTACCCGCACCCAAACCCAATCCCCCAACCGGGCGAGGGGACTGACCGCCTCCATTTTTTTTGTTGTCATAAGTCTGGTAGCCGCCCCTTGGCTTTAATTTTTTGTGAAAGAAATGTAAACaaaaact                                          >  1:1542294/1‑144 (MQ=255)
                                                                                                            ttCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGttt                                        <  1:207057/150‑1 (MQ=255)
                                                                                                              cGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCa                                      <  1:3500289/150‑1 (MQ=255)
                                                                                                                 aCCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCaaaa                                   >  2:741787/1‑150 (MQ=255)
                                                                                                                 aCCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCaaaa                                   <  2:1023187/150‑1 (MQ=255)
                                                                                                                  ccGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAat                                  <  1:1110481/150‑1 (MQ=255)
                                                                                                                  ccGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAATATTATGTCAAAGAAATGTAACCATTAAGTTTCAAAat                                  <  2:3219509/150‑1 (MQ=255)
                                                                                                                    gATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAatat                                <  2:1577708/150‑1 (MQ=255)
                                                                                                                      tGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGa                              <  1:1908714/150‑1 (MQ=255)
                                                                                                                         tGGCCTGTTACCCACACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAAACATAAAGTTTCAAAAAATGACct                           >  2:3551182/1‑150 (MQ=255)
                                                                                                                           gCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGCGGCCACGGGACTGACCCAGTCCAATTATGATGTTGTCAACAGTCTGGAACCCGCACCATCGCATTATTTTTATGTCAAAGAAATGTAACCATTAACTTTCAAAATATGACctct                         <  2:2595694/150‑1 (MQ=255)
                                                                                                                              tGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCAGTAAGTTTCAAAATATGACCTCTCtt                      >  2:3140321/1‑150 (MQ=255)
                                                                                                                                ttCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACGATTAAGTTTCAAAATATTTCCTCTCTTTa                    >  2:2459257/1‑150 (MQ=255)
                                                                                                                                ttCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTa                    >  2:3968461/1‑150 (MQ=255)
                                                                                                                                 tCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTaa                   <  1:1693585/150‑1 (MQ=255)
                                                                                                                                 tCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTaa                   <  1:909947/150‑1 (MQ=255)
                                                                                                                                    ccTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAAt                >  1:3581878/1‑150 (MQ=255)
                                                                                                                                     cTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTGTCTTTAAAATc               >  1:2627935/1‑150 (MQ=255)
                                                                                                                                     cTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATc               <  2:304947/150‑1 (MQ=255)
                                                                                                                                     cTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATc               >  1:3364953/1‑150 (MQ=255)
                                                                                                                                      tCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATcc              <  2:2696954/150‑1 (MQ=255)
                                                                                                                                       cACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTGTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCa             >  2:2554360/1‑150 (MQ=255)
                                                                                                                                       cACCCTAACCCACTCCCCAAACGCCGCGAGGGGACTGACCGAGTCCAATATTGATCTTGACAACAGTCTGGAAGCCGAACGTTGGCATTATATATATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCa             <  2:1756848/150‑1 (MQ=255)
                                                                                                                                        aCCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAg            >  1:3550527/1‑150 (MQ=255)
                                                                                                                                          ccTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCa          <  1:478982/150‑1 (MQ=255)
                                                                                                                                          ccTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTATGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAAACATTAAGTTTCAAAATATGACCTCTCTTTAAAATACAGCa          >  1:1301087/1‑150 (MQ=255)
                                                                                                                                           cTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCAt         >  2:4100806/1‑150 (MQ=255)
                                                                                                                                            tAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGAAATATCTTTAAAATCCATCAtt        >  2:2120363/1‑150 (MQ=255)
                                                                                                                                            tAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGGTGGCTTTATTTTTATGTCAAATAAATGTAACCATTAAGTTTCAAAATATTAACTCTCTTTAAAATACCGCAtt        >  1:742258/1‑150 (MQ=255)
                                                                                                                                                ccTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTc    >  2:1424107/1‑150 (MQ=255)
                                                                                                                                                 ctctCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCg   <  2:2573213/150‑1 (MQ=255)
                                                                                                                                                  tctcCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCAATAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCAATTTTCGc  >  1:2427669/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGC  >  NC_000913/3239532‑3239827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: