Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,121,583 Δ1 bp 6.2% intergenic (‑29/+26) wcaJ ← / ← cpsG colanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,121,5830G. 6.2% 339.0 / 16.6 112intergenic (‑29/+26)wcaJ/cpsGcolanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (58/45);  new base . (4/3);  total (64/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

Only 100 of 113 total aligned reads displayed.

CGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCC  >  NC_000913/2121434‑2121730
                                                                                                                                                     |                                                                                                                                                    
cGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCg                                                                                                                                                      >  2:7469/1‑150 (MQ=255)
    gACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGt                                                                                                                                                  >  2:437582/1‑150 (MQ=255)
      cTTCGCAAACCAGCCATAGTCCGTCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTgg                                                                                                                                               <  1:1811932/150‑1 (MQ=255)
       ttCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTggg                                                                                                                                              >  2:695665/1‑150 (MQ=255)
       ttCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTggg                                                                                                                                              <  2:62923/150‑1 (MQ=255)
       ttCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGGTGTTCCTGTTATTAGCCCCTTACCCGCAGTggg                                                                                                                                              >  1:1622848/1‑150 (MQ=255)
        tCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTgggg                                                                                                                                             >  1:380138/1‑150 (MQ=255)
        tCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTgggg                                                                                                                                             >  1:1393430/1‑150 (MQ=255)
         cGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGt                                                                                                                                            >  2:265840/1‑150 (MQ=255)
            aaaCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAg                                                                                                                                         <  1:775943/150‑1 (MQ=255)
             aaCCAGGCATAGTCCGGCAATCATGTTTGTGATATCTGAATAGCGTTGGACCATAGTGTTTTACGATTCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTGATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAgg                                                                                                                                        <  2:930826/150‑1 (MQ=255)
             aaCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAgg                                                                                                                                        <  1:1665486/150‑1 (MQ=255)
              aCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAggg                                                                                                                                       >  2:219304/1‑150 (MQ=255)
              aCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAggg                                                                                                                                       >  1:1759447/1‑150 (MQ=255)
              aCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGATATTAGCCCCTTACCCGCAGTGGGGTAAggg                                                                                                                                       <  2:1367557/150‑1 (MQ=255)
              aCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGAAATCGTTGTTCCTGTTATTAGCCCCTTCCCCGACGTGGGGTAAggg                                                                                                                                       >  2:1102900/1‑150 (MQ=255)
              aCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTAGCTCCCTCGCGCTTTTATAGATTTCTCATCGTTGTTCATGTTATTAGCCCCTTACACGCAGTGGGGTAAggg                                                                                                                                       >  1:317317/1‑150 (MQ=255)
               ccAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGa                                                                                                                                      >  2:1622585/1‑150 (MQ=255)
               ccAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAgggg                                                                                                                                      <  2:371487/150‑2 (MQ=255)
                cAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGaa                                                                                                                                     >  2:1026292/1‑150 (MQ=255)
                      tAGTCCGGCAAACATGTTGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGGGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCg                                                                                                                               <  1:901532/150‑1 (MQ=255)
                        gTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGAc                                                                                                                             <  2:1393430/150‑1 (MQ=255)
                         tCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACa                                                                                                                            <  1:1279159/150‑1 (MQ=255)
                           cGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGCTATTCGCTAGCTCGCCCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACACGCAGTGGGGTAAGGGAAGATCCGACAtt                                                                                                                          >  2:265140/1‑150 (MQ=255)
                            ggCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTa                                                                                                                         >  1:1803716/1‑150 (MQ=255)
                              cAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACt                                                                                                                       >  1:184899/1‑150 (MQ=255)
                                   atgatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTc                                                                                                                  <  2:1738994/150‑1 (MQ=255)
                                   atgatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGATTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTc                                                                                                                  <  1:861786/150‑1 (MQ=255)
                                    tgatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCa                                                                                                                 <  1:803666/150‑1 (MQ=255)
                                     tatgGGGTTATCTGATAAGAGTTGCACCATATTTATTTACGATGCTTTGGTTTTTGCTCGCTCGCGCTTTTTTAGATTTGTGATCGTTGTTCCTGTTATTAGCCACTTACCCGCAGTTGGGTAAGGGAAGTTCCGACATTACTCGTTCAg                                                                                                                <  1:1130865/149‑1 (MQ=255)
                                     gatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAg                                                                                                                <  1:1551608/150‑1 (MQ=255)
                                      ttgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATTGATTGGTTTTCGCTCGCTCTCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTTCCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGc                                                                                                               <  1:1729011/149‑1 (MQ=255)
                                         gTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAAc                                                                                                            >  1:1033072/1‑150 (MQ=255)
                                               tCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagc                                                                                                      <  2:813091/150‑1 (MQ=255)
                                                cTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagaa                                                                                                     >  1:190298/1‑148 (MQ=255)
                                                 tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagcag                                                                                                    <  2:1588385/150‑1 (MQ=255)
                                                 tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTCTCGTTCAGCAACGTCAGCaga                                                                                                   >  2:525328/1‑150 (MQ=255)
                                                              aCCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgttcgc                                                                                       >  1:1772361/1‑150 (MQ=255)
                                                               ccATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGGGttcgtgttcgcg                                                                                      <  1:1185461/150‑1 (MQ=255)
                                                               ccATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGttcgcgttcgcg                                                                                      <  1:1403877/150‑1 (MQ=255)
                                                                  ttgagaTTAACGATGCTTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCtt                                                                                   <  1:1166107/148‑1 (MQ=255)
                                                                       aTTAACGATGCATTGGTTATCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGGTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATc                                                                              <  2:1818855/150‑1 (MQ=255)
                                                                        atAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCa                                                                             <  2:1591193/149‑1 (MQ=255)
                                                                           aCGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCg                                                                          <  1:186231/150‑1 (MQ=255)
                                                                             gATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGc                                                                        >  1:103550/1‑150 (MQ=255)
                                                                                  aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATc                                                                   >  2:187710/1‑150 (MQ=255)
                                                                                  aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATc                                                                   >  1:883960/1‑150 (MQ=255)
                                                                                    tGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCAc                                                                 >  2:236704/1‑150 (MQ=255)
                                                                                     ggTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCAcc                                                                >  2:581705/1‑150 (MQ=255)
                                                                                      gtcttCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCAGTTAATAGCCCCTTACCCGCAGTGGCGTAAGGGAAGATCCGACATTACACGTTCAGCAACGGCCGCAGAGTTCGCGATCGCGCGTCCATCAGCGGCACATCACcg                                                               <  2:434123/147‑1 (MQ=255)
                                                                                       ttttCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACAACACcgc                                                              >  2:370711/1‑150 (MQ=255)
                                                                                        tttCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACcgcg                                                             >  2:1264936/1‑150 (MQ=255)
                                                                                          tcgctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACcgcgcg                                                           <  2:1541475/150‑1 (MQ=255)
                                                                                          tcgctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACcgagcg                                                           >  1:1113021/1‑150 (MQ=255)
                                                                                           cgctcgctcccGCTTTTTTACATATGACATCCTTGTACCTGTTATTAGCCCCATACGCCCTCTGGGCGATGGGATGATCCGACATTACTCGTTCAGCAGCGTCTGCAGCGATCGCGATCGCGCGTCCATCTGCGGCCCAGCACCGCTCGa                                                          >  2:762304/1‑150 (MQ=255)
                                                                                            gctcgctcgctcTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGAt                                                         >  2:1413069/1‑150 (MQ=255)
                                                                                            gctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGAt                                                         >  1:297092/1‑150 (MQ=255)
                                                                                            gctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGAt                                                         <  1:437582/150‑1 (MQ=255)
                                                                                             ctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGAtt                                                        <  1:768349/150‑1 (MQ=255)
                                                                                               ggctcgcGCTGTTTTAGATTTGTCAGCGTTCTTCCTGTTATTAGCCCCTTCCCCGCAGTGGTGTAAGGGAAGATCCGACATTACTCGTTCATCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCAAGAGCGGCACATCACCGCGCGATTcc                                                      <  1:75388/149‑1 (MQ=255)
                                                                                               cgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTcc                                                      >  2:199660/1‑150 (MQ=255)
                                                                                                   cgcgCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACAt                                                  >  1:204297/1‑150 (MQ=255)
                                                                                                   cgcgCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACAt                                                  >  1:754459/1‑150 (MQ=255)
                                                                                                      gcTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCa                                               <  1:1768924/150‑1 (MQ=255)
                                                                                                       cTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGTAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAg                                              >  2:540340/1‑150 (MQ=255)
                                                                                                        ttttttAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAgg                                             <  1:1031453/150‑1 (MQ=255)
                                                                                                          ttttAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGgcg                                           >  2:687250/1‑150 (MQ=255)
                                                                                                             tAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCACTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGcac                                        >  1:1302383/1‑150 (MQ=255)
                                                                                                              aGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGcacc                                       >  2:1091597/1‑150 (MQ=255)
                                                                                                                  ttGTCATCGTTGTTGCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCg                                   <  1:837310/150‑1 (MQ=255)
                                                                                                                      cATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTc                               >  2:11620/1‑150 (MQ=255)
                                                                                                                      cATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTc                               >  2:855405/1‑150 (MQ=255)
                                                                                                                       aTCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCg                              >  1:1440129/1‑150 (MQ=255)
                                                                                                                       aTCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCg                              >  2:1778647/1‑150 (MQ=255)
                                                                                                                       aTCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCg                              >  1:956931/1‑150 (MQ=255)
                                                                                                                        tCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCgg                             <  2:1033072/150‑1 (MQ=255)
                                                                                                                         cGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTCCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGt                            <  1:265840/150‑1 (MQ=255)
                                                                                                                             gttCCTGTTAATAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGCCCTTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGAACATCACCGCGCGATTCCACATTCAGCCGCACCACCGGTGCGGTATTg                        <  2:151152/150‑1 (MQ=255)
                                                                                                                              ttCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGAGCCGACATTACTCGTTCAGCACCGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTgg                       <  1:517235/150‑1 (MQ=255)
                                                                                                                              ttCCTGGTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTgg                       <  2:1759447/150‑1 (MQ=255)
                                                                                                                                ccTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGTCACATCAACGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAg                     >  1:775197/1‑150 (MQ=255)
                                                                                                                                   gTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCCGCGGCCCCTCACCGCGCGATTCCACATTCAGGCGCCCCACCGGTGCGGTAGTGGAGGTg                  >  2:1230989/1‑150 (MQ=255)
                                                                                                                                   gTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTg                  <  1:7469/150‑1 (MQ=255)
                                                                                                                                   gTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTg                  >  1:1256564/1‑150 (MQ=255)
                                                                                                                                         cgCCCCTTACCCGCAGTGGGTTAAGGGAAGATCCGACATTCCTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCCTCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg            <  1:906094/149‑1 (MQ=255)
                                                                                                                                         aGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCCCCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg            >  2:1392701/1‑150 (MQ=255)
                                                                                                                                         aGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCCCCCCCGGTTCGGTATTGGAGGTGCGCAgg            >  2:215254/1‑150 (MQ=255)
                                                                                                                                         aGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg            >  1:1755084/1‑150 (MQ=255)
                                                                                                                                          gCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGt           >  1:1226133/1‑150 (MQ=255)
                                                                                                                                          gCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGt           <  1:469704/150‑1 (MQ=255)
                                                                                                                                           tcccTTACCCCACTGCGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt          >  2:1295833/2‑150 (MQ=255)
                                                                                                                                           tcccTTACCCCACTGCGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt          >  1:691277/2‑150 (MQ=255)
                                                                                                                                            cccTTCCCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTa         <  2:1408613/150‑1 (MQ=255)
                                                                                                                                            cccTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCCCCACCGGTTCGGTATTGGAGGTGCGCAGGTTa         >  1:869114/1‑150 (MQ=255)
                                                                                                                                            cccTTACCCCACTGCGGGTAAGGGAAGCTCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTa         >  2:116573/1‑150 (MQ=255)
                                                                                                                                             ccTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaa        >  1:258684/1‑150 (MQ=255)
                                                                                                                                              cTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaaa       >  1:1784719/1‑150 (MQ=255)
                                                                                                                                               ttACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGATCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAg      <  1:219304/150‑1 (MQ=255)
                                                                                                                                                  cccGCAGGGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgcgc   <  2:1583928/150‑1 (MQ=255)
                                                                                                                                                   ccGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGcc  <  1:1816003/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCTGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCC  >  NC_000913/2121434‑2121730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: