Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,407,890 Δ1 bp 5.6% coding (330/1218 nt) alaA → glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,407,8900G. 5.6% 385.3 / 17.3 126coding (330/1218 nt)alaAglutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
Reads supporting (aligned to +/- strand):  ref base G (55/61);  new base . (5/2);  total (61/65)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

Only 100 of 129 total aligned reads displayed.

ACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGA  >  NC_000913/2407742‑2408039
                                                                                                                                                    |                                                                                                                                                      
aCCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAgg                                                                                                                                                       <  1:1494766/150‑1 (MQ=255)
aCCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGGCTTTACTCCGCGCGTAAAGCCATCATGCCGCACTACCAGGCTCGTGGCACGCGGGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAgg                                                                                                                                                       <  2:1826731/150‑1 (MQ=255)
 ccTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATTCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGc                                                                                                                                                      <  1:203333/150‑1 (MQ=255)
 ccTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGc                                                                                                                                                      >  2:612988/1‑150 (MQ=255)
  cTGCCAACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCCTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGAGTATCGGAGCTTATCGTTCAGGCa                                                                                                                                                     >  2:1553296/1‑150 (MQ=255)
         ccGCTCAAGGGTATTGCGATTCCATAGGGCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAgg                                                                                                                                              <  1:311728/150‑1 (MQ=255)
         ccGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAgg                                                                                                                                              >  2:1502831/1‑150 (MQ=255)
          cGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGc                                                                                                                                             <  2:1651908/150‑1 (MQ=255)
            cTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCAt                                                                                                                                           >  2:542458/1‑150 (MQ=255)
               aaGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGc                                                                                                                                       <  2:1364462/150‑1 (MQ=255)
               aaGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGc                                                                                                                                       <  2:732028/150‑1 (MQ=255)
                 gggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCATGCTCGTGGCATGCGTGATGTTACCGTGGAATATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTg                                                                                                                                     <  2:40564/150‑1 (MQ=255)
                 gggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTg                                                                                                                                     <  1:1020945/150‑1 (MQ=255)
                 gggTAGTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCGCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCCTGCATTGcct                                                                                                                                     <  2:1026798/150‑3 (MQ=255)
                  ggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCTGCAATGGTGTATCGGAGCTTATCGTTCAGTCAATGCAGGCATTGCTGa                                                                                                                                    >  1:883759/1‑150 (MQ=255)
                  ggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGa                                                                                                                                    >  1:570555/1‑150 (MQ=255)
                   gTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAAAGCCTGCATTGcctga                                                                                                                                   <  1:1266329/150‑5 (MQ=255)
                    tATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAAc                                                                                                                                  <  2:859102/150‑1 (MQ=255)
                      ttGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAg                                                                                                                                <  1:1367917/150‑1 (MQ=255)
                      ttGCGATTCCAAAGGTCTTTACTCCGCGCGT‑‑AGCCATCATGCAGCACTACCAGGCTCGTGGCCTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACTGCg                                                                                                                              >  2:285122/1‑150 (MQ=255)
                       tGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGCAATGCCTGCATTGCCTGAAcgat                                                                                                                               <  2:698306/150‑4 (MQ=255)
                        gCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCg                                                                                                                              >  1:600562/1‑150 (MQ=255)
                         cGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCgg                                                                                                                             >  1:1341220/1‑150 (MQ=255)
                          gATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCggg                                                                                                                            <  1:46606/150‑1 (MQ=255)
                              ccAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGCACAGCggggccc                                                                                                                        >  2:600859/1‑147 (MQ=255)
                              ccAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACg                                                                                                                        >  1:428729/1‑150 (MQ=255)
                              ccAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACg                                                                                                                        >  2:1133576/1‑150 (MQ=255)
                                      cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTg                                                                                                                <  1:1374877/150‑1 (MQ=255)
                                      cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTg                                                                                                                <  1:1502831/150‑1 (MQ=255)
                                      cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGGGGCATGCGTGATGTTACCGTGGAATATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCATGCAATGCAGGCATTGCTGAACTGCGTGGACGAAATGTTg                                                                                                                <  1:1464762/150‑1 (MQ=255)
                                       tttACTGAGCTCGTAAAGCCTTCATGCTGGTCTTACAGGCTTGTGTCATTCGTGTTGTTTCTGTGGAAGATATTTAGATCGGCTTTGGTTTATCGGAGCTTATCGTTCAGGCTATGCAGGCATTTCGGATCAGATGGGACGAAATGTTgg                                                                                                               <  2:328740/150‑1 (MQ=255)
                                       tttACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATTCAGGCATTGCTGAACAGCGGGGACGAAATGTTgg                                                                                                               <  1:1623122/150‑1 (MQ=255)
                                       tttACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTgg                                                                                                               <  2:328738/150‑1 (MQ=255)
                                         tactacGCGCGTATAGTCATAATGCAGCACTACCAGGGTCGTGGCATGCGTGATGTTACCGTGGAAGATATATACATCGGCAATGGGGTATCGGAGATTATCGTTCAGGAAATGGAGGCATTGCTGAACAGCGGGGACGTAATGTTGGtt                                                                                                             <  2:1114990/150‑1 (MQ=255)
                                         tACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGTCATTGATGAACAGAGGGGACGAAAtgtttgtt                                                                                                             >  2:1190269/1‑150 (MQ=255)
                                         tACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGtt                                                                                                             <  1:1509620/150‑1 (MQ=255)
                                              cgcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGTTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGc                                                                                                        <  1:1306994/150‑1 (MQ=255)
                                                cgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTAATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCAc                                                                                                      >  2:900651/1‑150 (MQ=255)
                                                     aaaGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATAATTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGAt                                                                                                 >  1:1205298/1‑150 (MQ=255)
                                                        gCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTAAGGCAATGCAGGCATTGCTGAACAGCGGGGAGGAAATGTTGGTTCCTGCAGCAGAttgc                                                                                              >  2:1309340/1‑148 (MQ=255)
                                                         ccATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAcc                                                                                             <  1:1095654/150‑1 (MQ=255)
                                                          catcatGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAccc                                                                                            <  1:1369184/150‑1 (MQ=255)
                                                              atGCAGCACTACCAGGCTCGTGGCATGCGTGAGGTAACCGTGGAAGATATTTACATCTGAATTGGTGTATCGGAGCGTATCGTTCAGGCAATGCAGGCATTGCTGTACAGCGGTGACGATATGTTGGTTCCTGCACCAGATTACCCActc                                                                                        <  1:1145951/150‑1 (MQ=255)
                                                               tGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCActct                                                                                       >  2:854436/1‑150 (MQ=255)
                                                               tGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCActct                                                                                       >  1:569631/1‑150 (MQ=255)
                                                                 cagcaCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCAATCTgg                                                                                     >  2:801711/1‑150 (MQ=255)
                                                                 cagcaCTACCAGGCTCGTGGCATGCGGGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTgg                                                                                     <  2:1028587/150‑1 (MQ=255)
                                                                    caCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGAcc                                                                                  >  2:1487955/1‑150 (MQ=255)
                                                                       tACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACcgcg                                                                               <  2:1048450/150‑1 (MQ=255)
                                                                        aCCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCACATTACCCACTCTGGACCgcgg                                                                              >  2:1200225/1‑150 (MQ=255)
                                                                         ccAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCgcggc                                                                             <  1:1145508/150‑1 (MQ=255)
                                                                         ccAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATAGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTTGTTGCTGCACCCGATAACCCACTCTGGACCgcggc                                                                             >  2:893601/1‑150 (MQ=255)
                                                                               tCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTc                                                                       >  1:641641/1‑150 (MQ=255)
                                                                                 gTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCATATTACCCACTCTGGACCGCGGCGGTTTCGc                                                                     <  1:1005432/150‑1 (MQ=255)
                                                                                   ggCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCtt                                                                   <  1:1782394/150‑1 (MQ=255)
                                                                                     cATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTc                                                                 <  1:834623/150‑1 (MQ=255)
                                                                                        gCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAg                                                              >  1:206923/1‑150 (MQ=255)
                                                                                           tGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCgg                                                           <  1:714794/150‑1 (MQ=255)
                                                                                            gATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGt                                                          >  2:532788/1‑150 (MQ=255)
                                                                                            gATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGt                                                          >  2:708664/1‑150 (MQ=255)
                                                                                            gATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACACACTCTGGACCGCGGCGTTTTCGCTTTCCAGCGGt                                                          >  2:566004/1‑150 (MQ=255)
                                                                                            gATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTCTCGTGCAGGCACTGCAGGCCTTGCGGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGt                                                          <  1:1554040/150‑1 (MQ=255)
                                                                                             aTGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTa                                                         <  2:346240/150‑1 (MQ=255)
                                                                                             aTGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTa                                                         >  1:597323/1‑150 (MQ=255)
                                                                                               gTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTaaa                                                       <  2:576864/150‑1 (MQ=255)
                                                                                                  aCCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCg                                                    >  2:900976/1‑150 (MQ=255)
                                                                                                   ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGTAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                   <  1:631638/150‑1 (MQ=255)
                                                                                                   ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                   >  2:687557/1‑150 (MQ=255)
                                                                                                   ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                   <  1:1681570/150‑1 (MQ=255)
                                                                                                   ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCGGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                   >  2:1098758/1‑150 (MQ=255)
                                                                                                      tGGAAGATAATTACACCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGc                                                <  1:1309340/150‑1 (MQ=255)
                                                                                                       ggAAGATATTTTCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCa                                               <  1:965891/150‑1 (MQ=255)
                                                                                                         aaGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCAtt                                             >  2:1720278/1‑150 (MQ=255)
                                                                                                          agagATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCATGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTTGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAATCGGTGCATTa                                            >  1:397268/1‑150 (MQ=255)
                                                                                                          aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTa                                            >  1:1719502/1‑150 (MQ=255)
                                                                                                                 ttgctCGGGAATGGTGTATGGGAGGTTATCGTTCTGGCAATGCAGGCATTGCTGAACAGGGGGGATGAAATGTTGGGTCCTGGACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGc                                     <  1:1553296/146‑1 (MQ=255)
                                                                                                                 aaCATCGGCAATGGTGTATCGGAGCTTATCGTTCACGCAATTCAGGCATTGCTGAACAGCGGTGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGCTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGc                                     <  1:268698/149‑1 (MQ=255)
                                                                                                                  aCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCg                                    <  1:449148/150‑1 (MQ=255)
                                                                                                                   cATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGa                                   >  1:322209/1‑150 (MQ=255)
                                                                                                                     tCGGCAATGGGGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGGTCCTGCACCAGATTACCCACTCTGGACCACGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATg                                 <  1:1273426/150‑1 (MQ=255)
                                                                                                                         cAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATc                             <  2:1746061/150‑1 (MQ=255)
                                                                                                                           aTGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCct                           <  2:239648/150‑1 (MQ=255)
                                                                                                                              gtgtATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTg                        <  2:1205298/150‑1 (MQ=255)
                                                                                                                                 tATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGTCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACt                     <  1:566004/150‑1 (MQ=255)
                                                                                                                                   tgggtgCTTATCGTTCAGGCAATGCAGGGATTGCTGAACAGAGGGGACGAAATGTTGGTTCCTGGACGAGATTACCCTCTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTgg                   <  1:1404537/145‑1 (MQ=255)
                                                                                                                                   tCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGATTCCTCTGACTgg                   >  1:1674138/1‑150 (MQ=255)
                                                                                                                                         cccgctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCg             >  1:1539990/7‑150 (MQ=255)
                                                                                                                                         cccgctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACACACACTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCg             >  1:639438/7‑150 (MQ=255)
                                                                                                                                          ccgctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCgg            >  1:1439020/6‑150 (MQ=255)
                                                                                                                                           cgctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGa           >  1:1579416/5‑150 (MQ=255)
                                                                                                                                            gctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc          >  1:1725057/4‑150 (MQ=255)
                                                                                                                                            aTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc          >  2:1738254/1‑150 (MQ=255)
                                                                                                                                               gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc       >  1:1643632/1‑150 (MQ=255)
                                                                                                                                               gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc       <  1:1331222/150‑1 (MQ=255)
                                                                                                                                               gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc       <  1:63297/150‑1 (MQ=255)
                                                                                                                                               gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc       >  1:929727/1‑150 (MQ=255)
                                                                                                                                                 tCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCga     >  2:1394359/1‑150 (MQ=255)
                                                                                                                                                 tCAGGCAATACAGGCATTGCTGACCAGCGGGGACGAAATGTTGGTTCATGCACCAGATTACCCACTATTGCCCGCTGCGGTTTCGCTTTCCATCGGTAAAGCGGTGCATTATCTTTGCGTCGAATCCTCTGACTGGTTGCCGGACCTAga     >  2:225001/1‑150 (MQ=255)
                                                                                                                                                  cAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGATCCTGCACCAGATTACCCACTCTGGACAGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTTCATTATCTTTGCGATGATTCCTCTGACTGGTTCCCGGCCCTCgat    >  1:1127402/1‑150 (MQ=255)
                                                                                                                                                    ggCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgatga  <  1:900651/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGA  >  NC_000913/2407742‑2408039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: