Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,757,939 Δ1 bp 5.2% intergenic (‑112/+78) yiaY ← / ← selB L‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,757,9390A. 5.2% 442.4 / 14.9 135intergenic (‑112/+78)yiaY/selBL‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor
Reads supporting (aligned to +/- strand):  ref base A (76/52);  new base . (5/2);  total (81/54)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

ACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCG  >  NC_000913/3757790‑3758088
                                                                                                                                                     |                                                                                                                                                      
aCATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTa                                                                                                                                                        >  1:940749/1‑150 (MQ=255)
 cATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTAt                                                                                                                                                       >  2:50544/1‑150 (MQ=255)
  aTTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTAtt                                                                                                                                                      <  2:561469/150‑1 (MQ=255)
     cacaGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAt                                                                                                                                                   >  2:199567/1‑150 (MQ=255)
     cacaGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAt                                                                                                                                                   <  1:1652457/150‑1 (MQ=255)
     aacaGAAGGAATAAAGAAAGTTGAAGCTGCCATAACACACTCCTGAAAGTTTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAt                                                                                                                                                   <  1:925888/149‑1 (MQ=255)
      acaGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAtt                                                                                                                                                  >  1:633512/1‑150 (MQ=255)
       caGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAGAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTa                                                                                                                                                 <  2:112531/150‑1 (MQ=255)
            ggAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                           >  1:1580324/1‑150 (MQ=255)
            ggAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                           >  1:40738/1‑150 (MQ=255)
            ggAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                           <  1:574472/150‑1 (MQ=255)
            ggAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                           >  2:595958/1‑150 (MQ=255)
            ggAATAAAGAACGTTGAAGCTGCCATAACACACTACTTAAAGTGTAAAAAAGTGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                           <  1:1742484/150‑1 (MQ=255)
             gAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAAt                                                                                                                                          >  1:82298/1‑150 (MQ=255)
             gAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATACATTAAACAAt                                                                                                                                          >  2:155295/1‑150 (MQ=255)
              aaTAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGAACATTGTATTCATTAAACAAtg                                                                                                                                         >  1:1167157/1‑150 (MQ=255)
                 aaaGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTTTAATGAATACAATGTGc                                                                                                                                      <  2:1445372/150‑1 (MQ=255)
                  aaGCACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATATAATAAAAAGCACATTGTTTAATGAATACAATGTGCt                                                                                                                                     <  2:204682/150‑1 (MQ=255)
                        gTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCttttttt                                                                                                                               >  2:581816/1‑150 (MQ=255)
                        gTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCttttttt                                                                                                                               >  1:1077179/1‑150 (MQ=255)
                         ttGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTa                                                                                                                              >  2:1209783/1‑150 (MQ=255)
                            aaGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCTAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAAt                                                                                                                           <  1:813140/150‑1 (MQ=255)
                              gCTGCCATAACACACTCCTGAAAGTGAAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGATTTTTTTAAAtta                                                                                                                         >  2:275608/1‑149 (MQ=255)
                               cTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCa                                                                                                                        <  1:581656/150‑1 (MQ=255)
                                 gCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATa                                                                                                                      >  2:1465545/1‑150 (MQ=255)
                                      aaCACAATCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGTATTTACATTCGATATTATGATCAGTATTATTGATTATAAAAATTAATCTAATAAAAAGATAATTGTATAAATTATTAAATCTGATTTTTTTAAATTCATAAGatt                                                                                                                 >  1:299429/1‑148 (MQ=255)
                                       acacacTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTACATACGAAAATATGATCAGTATTATTGATTATCAAAATTAAACTAATAAAAAGCACATTGTATTCATTAAAAAATGTCATTTTTTTAAATTAATAATAAtt                                                                                                                >  1:637714/1‑150 (MQ=255)
                                         acacTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAAtttt                                                                                                              >  2:1350122/1‑150 (MQ=255)
                                          cacTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTg                                                                                                             <  2:624309/150‑1 (MQ=255)
                                           aatacttaaaGTGTAAAAAATGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGt                                                                                                            <  2:509471/143‑1 (MQ=255)
                                             tCCTGAAAGTGTAAAAAAGGGCGGACAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTAt                                                                                                          >  2:1276648/1‑150 (MQ=255)
                                               cTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTa                                                                                                        >  1:839822/1‑150 (MQ=255)
                                                       gtAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAatttattt                                                                                                >  2:1708982/1‑150 (MQ=255)
                                                        tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                               >  1:1298104/1‑150 (MQ=255)
                                                        tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                               <  2:771099/150‑1 (MQ=255)
                                                        tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                               >  1:1019391/1‑150 (MQ=255)
                                                        tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTATTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                               >  2:725442/1‑150 (MQ=255)
                                                         aaaaaaGGTCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTa                                                                                              <  1:1606439/150‑1 (MQ=255)
                                                          aaaaaGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTaa                                                                                             <  1:378562/150‑1 (MQ=255)
                                                          aaaaaGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTaa                                                                                             >  2:790175/1‑150 (MQ=255)
                                                             aaGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAAc                                                                                          <  2:940749/150‑1 (MQ=255)
                                                               gggcgggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATGTAATAAAAAGCACATTGTATTAATTAAACTATTTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACtt                                                                                        <  2:391590/150‑1 (MQ=255)
                                                                ggcgggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACtta                                                                                       >  1:705947/1‑150 (MQ=255)
                                                                 gcgggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCAAAAGAATTTTTTATTAATTTATTTTAAAACttaa                                                                                      >  1:1385848/1‑150 (MQ=255)
                                                                    ggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACttaatta                                                                                   <  1:1503161/150‑1 (MQ=255)
                                                                    ggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACttaatta                                                                                   >  2:1251526/1‑150 (MQ=255)
                                                                       aGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTtaattaata                                                                                >  2:1594394/1‑149 (MQ=255)
                                                                        gCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCa                                                                               >  2:1617024/1‑150 (MQ=255)
                                                                                   aTTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAttt                                                                    >  2:525736/1‑150 (MQ=255)
                                                                                   aTTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAttt                                                                    <  1:1129233/150‑1 (MQ=255)
                                                                                    ttttCATTCGAAAATATGTTTATTATTATTTATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAtttt                                                                   <  2:782351/150‑1 (MQ=255)
                                                                                       tCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTcc                                                                <  2:342387/150‑1 (MQ=255)
                                                                                        cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                               <  2:82298/150‑1 (MQ=255)
                                                                                        cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                               <  2:777098/150‑1 (MQ=255)
                                                                                        cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                               <  1:49640/150‑1 (MQ=255)
                                                                                        cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                               <  2:209663/150‑1 (MQ=255)
                                                                                         aTTCGAAAATATTATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCgg                                                              <  2:239462/150‑1 (MQ=255)
                                                                                         aTTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCgg                                                              <  2:1500542/150‑1 (MQ=255)
                                                                                         aTTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCATTATTTTTcagt                                                              >  1:488945/1‑147 (MQ=255)
                                                                                           tCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATAATTTCATTATTTTTCAGGaa                                                            >  1:760721/1‑150 (MQ=255)
                                                                                           tCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGGGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGaa                                                            <  2:1139340/150‑1 (MQ=255)
                                                                                           acGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCATTATTTTTACGGaa                                                            >  1:760729/2‑150 (MQ=255)
                                                                                            cGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCATTATTTTTCCTGaaa                                                           >  1:1540373/1‑150 (MQ=255)
                                                                                               aaaTATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAaataat                                                       <  1:1475167/150‑1 (MQ=255)
                                                                                                   atGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAaataataat                                                    <  1:75085/150‑1 (MQ=255)
                                                                                                            attattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCgcgc                                           <  1:622794/150‑1 (MQ=255)
                                                                                                             ttattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCa                                          <  2:1738939/150‑1 (MQ=255)
                                                                                                                  gATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCGATaaa                                     >  2:1732768/1‑150 (MQ=255)
                                                                                                                  gATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATaaa                                     >  1:1618526/1‑150 (MQ=255)
                                                                                                                  gATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATaaa                                     >  2:1748428/1‑150 (MQ=255)
                                                                                                                  gATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATaaa                                     >  2:1670832/1‑150 (MQ=255)
                                                                                                                  gATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGAGCAATaaa                                     >  1:903372/1‑150 (MQ=255)
                                                                                                                  aaTTATCAAAATTAATCTAATAAAAAGCACATTGTAATAATTAAACAATGTGCTTTATATAAATAAATAAGAATTTTGTATTAATTTAAATTAAAAATTAATTAATCATTTCCTTATTTTTCCGGAAAAAATAATGCGACGCGCAATaaa                                     >  1:903370/2‑150 (MQ=255)
                                                                                                                     tATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGa                                  <  1:595958/150‑1 (MQ=255)
                                                                                                                     tATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGa                                  >  2:1140588/1‑150 (MQ=255)
                                                                                                                      aTCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGAt                                 <  2:1580324/150‑1 (MQ=255)
                                                                                                                        cAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCa                               >  2:636321/1‑150 (MQ=255)
                                                                                                                        aaaaaTTAATCTAATAAAAAGCACTTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCa                               <  2:850020/149‑1 (MQ=255)
                                                                                                                        aaaaaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCa                               <  1:523702/149‑1 (MQ=255)
                                                                                                                         aaaaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATAAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCAt                              >  2:372857/1‑150 (MQ=255)
                                                                                                                           aaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCAttt                            >  1:1733443/1‑150 (MQ=255)
                                                                                                                               aaTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCac                        <  2:1298104/150‑1 (MQ=255)
                                                                                                                                aTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                       <  1:1029805/150‑1 (MQ=255)
                                                                                                                                aTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                       >  1:656700/1‑150 (MQ=255)
                                                                                                                                  cTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgac                     >  1:617387/1‑150 (MQ=255)
                                                                                                                                  cTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTACCGGAAATAATAATGCTTCGCGCAATAAATCATCATTTCCacgac                     >  1:1458873/1‑150 (MQ=255)
                                                                                                                                   taataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGTAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacg                    >  1:1325268/1‑150 (MQ=255)
                                                                                                                                    taaaaaaaGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacga                   >  2:1057652/4‑150 (MQ=38)
                                                                                                                                    taaaaaaaGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCATTATTTTTCCGGAAATAATAATGAGTCGCGCAATAAATGATCATTTCAacgacga                   >  2:1057648/4‑150 (MQ=38)
                                                                                                                                     ataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTACGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacgac                  >  2:281534/1‑150 (MQ=255)
                                                                                                                                      taaAAAGCACATTTTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGTATTTTGTATTAATTTAATTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacgacg                 <  1:316744/150‑1 (MQ=255)
                                                                                                                                          aaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTa             <  1:1209783/150‑1 (MQ=255)
                                                                                                                                          aaGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTa             >  1:703561/1‑150 (MQ=255)
                                                                                                                                           aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa            >  2:412643/1‑150 (MQ=255)
                                                                                                                                           aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa            >  2:1029959/1‑150 (MQ=255)
                                                                                                                                            gCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCAGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACTAGTaaa           >  1:363697/1‑150 (MQ=255)
                                                                                                                                             cacaTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAg          >  2:318329/1‑150 (MQ=255)
                                                                                                                                             cacaTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAg          >  2:719440/1‑150 (MQ=255)
                                                                                                                                              acaTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGc         >  2:406457/1‑150 (MQ=255)
                                                                                                                                                   gTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATg    >  2:719867/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCG  >  NC_000913/3757790‑3758088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: