Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,799,374:1 +T 6.0% intergenic (‑104/+19) pheM ← / ← rplT phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,799,3741.T 6.0% 459.0 / 23.3 117intergenic (‑104/+19)pheM/rplTphenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20
Reads supporting (aligned to +/- strand):  ref base . (51/59);  new base T (2/5);  total (53/64)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

TTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGT  >  NC_000913/1799225‑1799523
                                                                                                                                                      |                                                                                                                                                     
ttCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAg                                                                                                                                                        >  2:821322/1‑150 (MQ=255)
ttCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAg                                                                                                                                                        >  1:1709470/1‑150 (MQ=255)
 tCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGc                                                                                                                                                      >  1:942461/1‑150 (MQ=255)
     gTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTccc                                                                                                                                                  <  1:1107269/150‑1 (MQ=255)
       gCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCctc                                                                                                                                                <  1:1039235/150‑1 (MQ=255)
       gCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCctc                                                                                                                                                <  2:90853/150‑1 (MQ=255)
       gCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCctc                                                                                                                                                >  1:499413/1‑150 (MQ=255)
         tAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTAAATGTTTTTATGGCGTTTAAACGAAAAGAGGGAGACTAGCTCCCTCtt                                                                                                                                              >  1:349895/1‑150 (MQ=255)
          aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCttt                                                                                                                                             >  1:1035852/1‑150 (MQ=255)
          aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCttt                                                                                                                                             >  2:45145/1‑150 (MQ=255)
          aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCttt                                                                                                                                             >  2:1616898/1‑150 (MQ=255)
          aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCttt                                                                                                                                             >  2:1268324/1‑150 (MQ=255)
           aaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTc                                                                                                                                            <  1:1004148/150‑1 (MQ=255)
            aGTAAATAACGAAGCGGAAAATTGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTGTTCAAAAGCGCTTTATTGATAAGGCTTCGGCGATCAAAGTCCATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGCTAGTCTCCCTCttttc                                                                                                                                           <  1:259731/150‑3 (MQ=255)
            aGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCa                                                                                                                                           >  1:771865/1‑150 (MQ=255)
            aGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCa                                                                                                                                           >  1:578219/1‑150 (MQ=255)
             ttAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGCTAGTCTCCCTCttttcg                                                                                                                                          <  1:973545/149‑4 (MQ=255)
             gTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCaa                                                                                                                                          >  2:1727173/1‑150 (MQ=255)
             gTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGCTAGTCTCCCTCttttcg                                                                                                                                          <  2:840030/150‑4 (MQ=255)
              tAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGCTAGTCTCCCTCttttcgt                                                                                                                                         <  1:1540541/150‑5 (MQ=255)
              aaaaaaaaGAAGCGGAAAATAGCAGCATACATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAAc                                                                                                                                         >  2:712039/2‑150 (MQ=255)
               aaaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGCTAGTCTCCCTCttttcgtt                                                                                                                                        <  1:839711/150‑6 (MQ=255)
                  aaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAATTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGc                                                                                                                                     >  2:299099/1‑150 (MQ=255)
                       aagCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATg                                                                                                                                <  1:640592/150‑1 (MQ=255)
                           ggAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCag                                                                                                                            <  2:977566/150‑1 (MQ=255)
                           ggAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCag                                                                                                                            >  1:782816/1‑150 (MQ=255)
                                aTAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAgctg                                                                                                                       <  1:119855/150‑1 (MQ=255)
                                 tAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAgctgc                                                                                                                      <  1:829957/150‑1 (MQ=255)
                                 tAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCATAAGCCagttgaaa                                                                                                                      <  2:791380/150‑7 (MQ=255)
                                   gcagcaTTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACAGGCTTATGCAAGAGCTGAtt                                                                                                                    >  2:501055/1‑150 (MQ=255)
                                    cagcaTTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCttt                                                                                                                   <  1:694103/150‑1 (MQ=255)
                                    cagcaTTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCAGAGCTGCTTTc                                                                                                                  <  2:272319/150‑1 (MQ=255)
                                      gcaTTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAATCCCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCg                                                                                                                 <  2:1632379/150‑1 (MQ=255)
                                      gcaTTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCg                                                                                                                 <  1:560955/150‑1 (MQ=255)
                                       cattctttGCTTGCGTTACCTTTTGGTACTCTTCATAAGACCTTTATTGAATAGGCTACGGCGATAAAAGTCAATTTTTTGTTGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGc                                                                                                                <  1:1241679/150‑1 (MQ=255)
                                           cattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtttt                                                                                                            >  2:796400/1‑150 (MQ=255)
                                            attGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCttttt                                                                                                           >  1:538886/1‑150 (MQ=255)
                                            attGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCttttt                                                                                                           <  2:286590/150‑1 (MQ=255)
                                             ttgcttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTc                                                                                                          >  1:707379/1‑150 (MQ=255)
                                             ttgcttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTc                                                                                                          >  1:713739/1‑150 (MQ=255)
                                             ttgcttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGGATATGTTTTGATGGCGTTGAAACGTAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTc                                                                                                          <  2:1193662/150‑1 (MQ=255)
                                               gcttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCaa                                                                                                        >  1:1751006/1‑150 (MQ=255)
                                                    cGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCaga                                                                                                   >  2:934575/1‑150 (MQ=255)
                                                     gTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCagag                                                                                                  >  1:323558/1‑150 (MQ=255)
                                                       tACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCg                                                                                                <  2:1575522/150‑1 (MQ=255)
                                                        aCCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCgg                                                                                               >  2:980319/1‑150 (MQ=255)
                                                        aCCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCgg                                                                                               <  2:323558/150‑1 (MQ=255)
                                                             ttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAAc                                                                                          >  1:1206237/1‑150 (MQ=255)
                                                              tGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACg                                                                                         <  2:189290/150‑1 (MQ=255)
                                                                 tACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTa                                                                                      <  2:702204/150‑1 (MQ=255)
                                                                  aCTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTACCTCTTTCAACTGGATTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTaa                                                                                     >  1:416097/1‑149 (MQ=255)
                                                                     ctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                                  >  1:1746166/1‑150 (MQ=255)
                                                                       tCAAAAGACCTTTATTGAAATGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGTAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt                                                                                <  2:576501/150‑1 (MQ=255)
                                                                       tCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt                                                                                <  2:1240874/150‑1 (MQ=255)
                                                                       tCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt                                                                                <  1:576905/150‑1 (MQ=255)
                                                                        cAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc                                                                               <  2:222692/150‑1 (MQ=255)
                                                                         aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCg                                                                              >  1:1780748/1‑150 (MQ=255)
                                                                         aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCg                                                                              <  1:236132/150‑1 (MQ=255)
                                                                         aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCg                                                                              <  2:687344/150‑1 (MQ=255)
                                                                                 tttATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGc                                                                      <  1:1760725/150‑1 (MQ=255)
                                                                                   tATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGa                                                                    <  1:1062249/150‑1 (MQ=255)
                                                                                    aTTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGat                                                                   <  2:1035852/150‑1 (MQ=255)
                                                                                     ttGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGata                                                                  <  1:323509/150‑1 (MQ=255)
                                                                                     ttGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCATTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGata                                                                  >  1:688569/1‑150 (MQ=255)
                                                                                       gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGatata                                                                >  1:299510/1‑149 (MQ=255)
                                                                                       gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATc                                                                >  1:716146/1‑150 (MQ=255)
                                                                                       gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATc                                                                >  2:207685/1‑150 (MQ=255)
                                                                                       gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTAGAATACTGCGATATc                                                                >  2:1079252/1‑150 (MQ=255)
                                                                                        aaaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGTGGTGAACGCTACTTTGTCGAATACTGCGATATCa                                                               >  1:1377806/1‑150 (MQ=255)
                                                                                              cTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTGTCGCTTTTTCAACCAGAGCGGTGCACGCTACTTTGTCGAATACTGCGATATCCGCCAgg                                                         >  2:76158/1‑150 (MQ=255)
                                                                                                    cGATAAAAGTCAATGTTTTGATGGCGTTGCAACGAAAAGAGGGAGACTAGCTCCCTCTTACAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTAGAATTCTGCGATATCAGCCAGGATCTTa                                                   >  2:272479/1‑150 (MQ=255)
                                                                                                       tAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACgg                                                >  2:582690/1‑150 (MQ=255)
                                                                                                         aaaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTc                                              <  1:1616898/150‑1 (MQ=255)
                                                                                                           aGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGa                                            >  2:1767952/1‑150 (MQ=255)
                                                                                                            gTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATTCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAt                                           <  1:267289/150‑1 (MQ=255)
                                                                                                            gTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAt                                           <  1:856982/150‑1 (MQ=255)
                                                                                                            gTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAtt                                          <  2:1202297/150‑1 (MQ=255)
                                                                                                              cAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATACCAGCCAGGATCTTACGGTCGAttt                                         >  2:1478208/1‑150 (MQ=255)
                                                                                                               aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTc                                        <  2:1193828/150‑1 (MQ=255)
                                                                                                                aTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCa                                       <  2:1173339/150‑1 (MQ=255)
                                                                                                                 tGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTCCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCaa                                      <  1:299798/150‑1 (MQ=255)
                                                                                                                  gTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAAc                                     <  1:637413/150‑1 (MQ=255)
                                                                                                                  gTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACCGCGATATCAGCCAGGATCTTACGGTCGATTTCAAc                                     >  1:17557/1‑150 (MQ=255)
                                                                                                                   ttttGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACa                                    >  2:666335/1‑150 (MQ=255)
                                                                                                                      tGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACagag                                 <  2:378349/150‑1 (MQ=255)
                                                                                                                       gATGGCTTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAgg                                <  1:1608088/150‑1 (MQ=255)
                                                                                                                       gATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTATGGTCGATTTCAACAGAgg                                >  1:1274444/1‑150 (MQ=255)
                                                                                                                            cGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCtttt                           >  1:82159/1‑150 (MQ=255)
                                                                                                                             gTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCttttt                          >  2:642258/1‑150 (MQ=255)
                                                                                                                             gTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGACGATTTCAACAGACGCttttt                          >  1:753967/1‑150 (MQ=255)
                                                                                                                                 aaaCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAg                      <  1:45145/150‑1 (MQ=255)
                                                                                                                                 aaaCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAg                      <  1:1268324/150‑1 (MQ=255)
                                                                                                                                     gAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCa                  >  2:618297/1‑150 (MQ=255)
                                                                                                                                      gaaaGAGGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCCCCCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCAt                 >  1:282576/2‑150 (MQ=255)
                                                                                                                                          gagGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtgat             <  2:782816/150‑1 (MQ=255)
                                                                                                                                           agGGAGACAAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACAAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTAACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtgatg            <  1:66241/150‑1 (MQ=255)
                                                                                                                                              gagaCTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAAt         >  1:896998/1‑150 (MQ=255)
                                                                                                                                              gagaCTAGCTCCCTCTTTAAACTGTCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGGGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAAt         <  1:50275/150‑1 (MQ=255)
                                                                                                                                                gaCTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAAttt       <  2:385803/150‑1 (MQ=255)
                                                                                                                                                    aGCTCCCTCTTTCAACTGGCTTTTGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACCCTACTTTGTCCAATCCTTCGATATCAGCCAGGATCTTACTGTCGATTTCAACAGAGGCTTTTTTGATGCCATTGATGAATTTGCTg   <  2:3617/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGT  >  NC_000913/1799225‑1799523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: