Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 645,069 G→A 6.2% intergenic (‑66/+48) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913645,0690GA 6.2% 409.9 / 9.7 129intergenic (‑66/+48)rna/citTribonuclease I/citrate/succinate antiporter; citrate carrier
Reads supporting (aligned to +/- strand):  ref base G (48/73);  new base A (2/6);  total (50/79)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-01

Only 100 of 134 total aligned reads displayed.

TGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCAC  >  NC_000913/644920‑645218
                                                                                                                                                     |                                                                                                                                                     
 gCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGTGAAGCAGAGAAACCTCGAGAAACGCGGCGTTACGCCAGAATGCTTTAATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTgg                                                                                                                                                      <  1:100691/150‑1 (MQ=255)
   cTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGc                                                                                                                                                    >  2:820038/1‑150 (MQ=255)
   cTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCCAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGc                                                                                                                                                    >  1:494007/1‑150 (MQ=255)
     gCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCACGCCAGGTGGGCtt                                                                                                                                                  >  2:203591/1‑150 (MQ=255)
      cAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTa                                                                                                                                                 <  2:138749/150‑1 (MQ=255)
      cAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTa                                                                                                                                                 >  2:561550/1‑150 (MQ=255)
      cAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTa                                                                                                                                                 >  2:1427138/1‑150 (MQ=255)
       aaCGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTAc                                                                                                                                                <  2:1215942/150‑1 (MQ=255)
         cGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTAACTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCt                                                                                                                                              >  1:154186/1‑150 (MQ=255)
          gccttagCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTg                                                                                                                                             <  2:795255/144‑1 (MQ=255)
           cTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTgg                                                                                                                                            >  1:853649/1‑150 (MQ=255)
             aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                          <  2:1825908/150‑1 (MQ=255)
              tagCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcgg                                                                                                                                         <  1:1274152/148‑1 (MQ=255)
              aGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcgg                                                                                                                                         <  1:814238/150‑1 (MQ=255)
               ggggTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcggg                                                                                                                                        <  2:1499398/147‑1 (MQ=255)
               ggCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcggg                                                                                                                                        <  2:880308/150‑1 (MQ=255)
               ggCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcggg                                                                                                                                        <  1:1236624/150‑1 (MQ=255)
               ggCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcggg                                                                                                                                        <  1:535848/150‑1 (MQ=255)
                gCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcgggc                                                                                                                                       <  2:694246/150‑1 (MQ=255)
                gCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcgggc                                                                                                                                       <  1:272970/150‑1 (MQ=255)
                gCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcgggc                                                                                                                                       <  2:749470/150‑1 (MQ=255)
                 cGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcgggcg                                                                                                                                      <  1:970813/150‑1 (MQ=255)
                 cGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcgggct                                                                                                                                      <  2:588308/150‑2 (MQ=255)
                 cGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcgggct                                                                                                                                      <  1:90830/150‑2 (MQ=255)
                     ggCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGtgat                                                                                                                                  >  2:1637669/1‑150 (MQ=255)
                      gCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGtgatg                                                                                                                                 <  1:264709/150‑1 (MQ=255)
                       cAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGtgatga                                                                                                                                >  1:1286004/1‑150 (MQ=255)
                       cAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGtgatga                                                                                                                                <  2:367409/150‑1 (MQ=255)
                         taagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGAtt                                                                                                                              <  2:1634947/149‑1 (MQ=255)
                         gaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGAtt                                                                                                                              <  2:900763/150‑1 (MQ=255)
                          aagagaagGGAAGCACAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGTTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGAttt                                                                                                                             <  1:766451/150‑1 (MQ=255)
                           agagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTa                                                                                                                            >  1:1401937/1‑150 (MQ=255)
                            gagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTAt                                                                                                                           <  1:1486272/150‑1 (MQ=255)
                              gaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTc                                                                                                                         <  1:305389/150‑1 (MQ=255)
                              gaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTc                                                                                                                         <  1:439153/150‑1 (MQ=255)
                              gaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTc                                                                                                                         <  2:954618/150‑1 (MQ=255)
                              gaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAAAGCTTTCATAATGCGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTc                                                                                                                         <  1:40235/150‑1 (MQ=255)
                                     aGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGttt                                                                                                                  >  2:616994/1‑150 (MQ=255)
                                       cAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTgg                                                                                                                >  1:1694953/1‑150 (MQ=255)
                                       cAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTgg                                                                                                                >  2:1399935/1‑150 (MQ=255)
                                         gagaAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCg                                                                                                              <  2:872203/150‑1 (MQ=255)
                                              aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                         <  1:820038/150‑1 (MQ=255)
                                               ccGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGCACGTa                                                                                                        >  1:516933/1‑150 (MQ=255)
                                                cgcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTAt                                                                                                       <  1:1151223/150‑1 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACTCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                      <  2:1079706/150‑1 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                      <  1:273997/150‑1 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                      >  1:1083181/1‑150 (MQ=255)
                                                   gAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAg                                                                                                    <  1:997968/150‑1 (MQ=255)
                                                   gAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACAATGATTACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGTGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAg                                                                                                    <  1:295805/150‑1 (MQ=255)
                                                      cAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGAGGGCTTACCTGGCGGGCGTGATGCTTTTTTCAGCGTTTGGCTAACGTATTAGTTc                                                                                                 >  2:1658451/1‑150 (MQ=255)
                                                       aaCGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTcc                                                                                                <  1:637623/150‑1 (MQ=255)
                                                        aCGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCca                                                                                               <  1:339972/150‑1 (MQ=255)
                                                          gcggcgTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCcaca                                                                                             <  1:799343/150‑1 (MQ=255)
                                                                 tACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCgag                                                                                      <  2:791254/150‑1 (MQ=255)
                                                                      cAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCg                                                                                 <  2:86008/150‑1 (MQ=255)
                                                                           tGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                            <  1:1025340/150‑1 (MQ=255)
                                                                            gCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagc                                                                           <  2:1455448/150‑1 (MQ=255)
                                                                              tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                         <  1:1454885/150‑1 (MQ=255)
                                                                                 cATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaacc                                                                      <  2:192582/150‑1 (MQ=255)
                                                                                  aTAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaacca                                                                     >  1:896499/1‑150 (MQ=255)
                                                                                    aaTGGGTGTGGAACTCATACATACACTGTATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCcaaccaac                                                                   >  2:340384/1‑150 (MQ=255)
                                                                                    aaTGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCcaaccaac                                                                   <  2:258217/150‑1 (MQ=255)
                                                                                       gggTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTGTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGc                                                                <  2:1237307/150‑1 (MQ=255)
                                                                                         gtgtGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCaa                                                              >  2:634268/1‑150 (MQ=255)
                                                                                          tgtgGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAAc                                                             >  2:1420460/1‑150 (MQ=255)
                                                                                            tgGAACTCATCCATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACAt                                                           <  2:290064/150‑1 (MQ=255)
                                                                                             ggAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGACGACAATCGGCCAGCCACCCACCAGCAACATa                                                          >  2:920335/1‑150 (MQ=255)
                                                                                                       catacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTaaa                                                >  1:1568709/1‑150 (MQ=255)
                                                                                                        atacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAAt                                               >  1:753804/1‑150 (MQ=255)
                                                                                                         tacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATc                                              <  1:1088850/150‑1 (MQ=255)
                                                                                                         tacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATc                                              <  2:756930/150‑1 (MQ=255)
                                                                                                         tacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATc                                              <  1:851839/150‑1 (MQ=255)
                                                                                                          acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATcc                                             >  1:325039/1‑149 (MQ=255)
                                                                                                            acTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcc                                           <  1:1641159/150‑1 (MQ=255)
                                                                                                             cTGAATACAATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCACGCGGGCGTGATGATTTATTCAGCGATAGGCGAACTGATTAGGACCACATGGCGAGACTCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAccc                                          <  1:308181/150‑1 (MQ=255)
                                                                                                              tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                         >  1:1160146/1‑150 (MQ=255)
                                                                                                               gAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCg                                        >  2:1435989/1‑150 (MQ=255)
                                                                                                                   aCTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGa                                    <  1:616994/150‑1 (MQ=255)
                                                                                                                       tctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgc                                <  1:757956/150‑1 (MQ=255)
                                                                                                                          tttAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATAGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGcc                             <  2:1215365/149‑1 (MQ=255)
                                                                                                                                tCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCTACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACg                       >  2:1034163/1‑150 (MQ=255)
                                                                                                                                tCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGCTTGCGCCAAGAcc                       >  2:465193/1‑149 (MQ=255)
                                                                                                                                tCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAACACg                       >  2:1041196/1‑150 (MQ=255)
                                                                                                                                  aTAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGcc                     <  2:494007/150‑1 (MQ=255)
                                                                                                                                  aTAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGGATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGcc                     >  2:973740/1‑150 (MQ=255)
                                                                                                                                    aaaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAg                   <  1:539248/150‑1 (MQ=255)
                                                                                                                                         aGCCTGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCTCCAAGACGCCAGTAATc              <  1:304946/150‑1 (MQ=255)
                                                                                                                                          gCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCt             >  2:1507054/1‑150 (MQ=255)
                                                                                                                                           cccGCCAGGTGGGCTTACCTGGCGGGAGTGATGATTAATTAAGAGTTTGGAAAAAGTATTATTTCCACATGGAGAGAATCGGCCAGCCAACCAAAATAAACATAGAGATGTAAATCACCCCGAAGATTTCGCCAAGCCGCCAGGAATCtt            >  2:24387/1‑150 (MQ=255)
                                                                                                                                            ccGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt           >  1:162472/1‑150 (MQ=255)
                                                                                                                                            ccGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGGAAGCttt           >  2:620780/1‑150 (MQ=255)
                                                                                                                                            ccGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGATCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt           >  1:1418395/1‑150 (MQ=255)
                                                                                                                                             cGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt          <  1:399324/150‑1 (MQ=255)
                                                                                                                                             cGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt          <  2:1603940/150‑1 (MQ=255)
                                                                                                                                              gCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTTATCTTTTg         >  2:976811/1‑150 (MQ=255)
                                                                                                                                              gCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTg         >  1:920303/1‑150 (MQ=255)
                                                                                                                                              gCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTg         <  1:990227/150‑1 (MQ=255)
                                                                                                                                              gCCAGGTAAGCCCACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCCCATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTg         >  1:450618/1‑150 (MQ=255)
                                                                                                                                                    tGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTca   >  1:399909/1‑150 (MQ=255)
                                                                                                                                                     gggCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTcac  <  1:625294/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCAC  >  NC_000913/644920‑645218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: