Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 654,563 C→G 7.5% intergenic (+21/+20) citB → / ← dcuC response regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913654,5630CG 7.5% 448.4 / 20.9 134intergenic (+21/+20)citB/dcuCresponse regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base C (57/67);  new base G (7/3);  total (64/70)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 139 total aligned reads displayed.

CAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATC  >  NC_000913/654414‑654712
                                                                                                                                                     |                                                                                                                                                      
cAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAAc                                                                                                                                                        <  2:364781/150‑1 (MQ=255)
    cACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGAACACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGc                                                                                                                                                    >  2:1401952/1‑150 (MQ=255)
      cTGACCATCAGCCGCACCACTGCCTGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCt                                                                                                                                                  <  1:1546090/150‑1 (MQ=255)
       tGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCGATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGAAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCCGAGGAAACTTGCCTc                                                                                                                                                 >  1:1070117/1‑150 (MQ=255)
       tGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGCCCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTc                                                                                                                                                 >  1:996932/1‑150 (MQ=255)
          ccATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTAACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCgg                                                                                                                                              >  1:423601/1‑150 (MQ=255)
           cagcAGCCGCACCACTGCCAGGCGTTATCTTGAAGATCGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                                                                             <  1:301543/147‑1 (MQ=255)
           cATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGc                                                                                                                                             <  2:1156224/150‑1 (MQ=255)
           cATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGc                                                                                                                                             <  1:370213/150‑1 (MQ=255)
               aGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTa                                                                                                                                         >  2:1374536/1‑150 (MQ=255)
                  cGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTa                                                                                                                                      >  1:1643705/1‑150 (MQ=255)
                  cGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTa                                                                                                                                      >  2:1191819/1‑150 (MQ=255)
                   gCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAt                                                                                                                                     <  2:628267/150‑1 (MQ=255)
                    caccacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAtt                                                                                                                                    <  2:1058588/150‑1 (MQ=255)
                     accacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAATGCCGTAGGCAAGTTTCCTCCGGCTTTatcatgt                                                                                                                                   <  1:1037880/150‑7 (MQ=255)
                     accacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAATGCCGGAGGCAAGTTTCCTCCGGCTTTatcatgt                                                                                                                                   <  1:947173/150‑7 (MQ=39)
                     accacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAttt                                                                                                                                   >  1:713752/1‑150 (MQ=255)
                     accacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAttt                                                                                                                                   <  1:480940/150‑1 (MQ=255)
                      ccacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTa                                                                                                                                  >  1:548028/1‑150 (MQ=255)
                       cacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTAc                                                                                                                                 >  1:1060763/1‑150 (MQ=255)
                       cacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTAc                                                                                                                                 <  1:1802269/150‑1 (MQ=255)
                        acTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACt                                                                                                                                <  2:634848/150‑1 (MQ=255)
                          tGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTg                                                                                                                              <  1:838029/150‑1 (MQ=255)
                           gCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGc                                                                                                                             >  1:173820/1‑150 (MQ=255)
                            ccAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGcc                                                                                                                            <  1:1539531/150‑1 (MQ=255)
                                gCGTTATCTTGAATATTGCGGCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGTCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCtgtg                                                                                                                        <  1:74067/150‑1 (MQ=255)
                                     aTCTTGAATATTGAGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGAcctc                                                                                                                   >  1:858844/1‑148 (MQ=255)
                                       cTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                                 >  2:1008736/1‑150 (MQ=255)
                                       cTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                                 <  2:972522/150‑1 (MQ=255)
                                        ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctgc                                                                                                                >  1:561100/1‑150 (MQ=255)
                                         tGAATATTGCTCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctgct                                                                                                               <  1:901836/150‑1 (MQ=255)
                                          gAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGctgctg                                                                                                              >  2:468505/1‑150 (MQ=255)
                                            atatTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGAGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCACTGCTGcc                                                                                                            >  1:1204980/1‑150 (MQ=255)
                                               ttGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGtt                                                                                                         >  2:934761/1‑150 (MQ=255)
                                                 gcgcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTcc                                                                                                       >  2:1475737/1‑150 (MQ=255)
                                                 gcgcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCGCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTcc                                                                                                       <  1:911343/150‑1 (MQ=255)
                                                  cgcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCt                                                                                                      <  1:1024324/150‑1 (MQ=255)
                                                   gcCAGCCGCCTTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCAGTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTg                                                                                                     >  1:398618/1‑150 (MQ=255)
                                                   gcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTg                                                                                                     <  2:1753528/150‑1 (MQ=255)
                                                     cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGc                                                                                                   <  2:133522/150‑1 (MQ=255)
                                                       gccgccATCTTATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAc                                                                                                 <  2:752995/150‑1 (MQ=255)
                                                        ccgccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAcc                                                                                                <  2:389917/150‑1 (MQ=255)
                                                            cATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCa                                                                                            <  1:428137/150‑1 (MQ=255)
                                                              tCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGc                                                                                          <  2:1594403/150‑1 (MQ=255)
                                                              tCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGc                                                                                          >  2:857727/1‑150 (MQ=255)
                                                              tCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGACAGACCACAACGCATATACCACAGTTGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGACGTTCCTGGCACCATCAGc                                                                                          <  2:713475/150‑1 (MQ=255)
                                                                tGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctc                                                                                        <  1:1376987/150‑1 (MQ=255)
                                                                 gATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTGCTGGCACCATCAGctca                                                                                       >  2:1213797/1‑149 (MQ=255)
                                                                 gATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctct                                                                                       >  1:1215640/1‑150 (MQ=255)
                                                                  attattGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATTCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTg                                                                                      <  1:135499/150‑1 (MQ=255)
                                                                    tattGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTa                                                                                    <  1:1058851/150‑1 (MQ=255)
                                                                        gCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCaa                                                                                >  1:7927/1‑150 (MQ=255)
                                                                        gCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGGGCCCGCTGCTGCCGTGCCGGGCACCAGCCGCTCTGTAGCac                                                                                >  2:462632/1‑149 (MQ=255)
                                                                          tGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACg                                                                              <  1:347198/150‑1 (MQ=255)
                                                                           gAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGa                                                                             >  1:505142/1‑150 (MQ=255)
                                                                            aaaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGAt                                                                            <  2:1437213/150‑1 (MQ=255)
                                                                              aTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATaa                                                                          <  1:1362316/150‑1 (MQ=255)
                                                                              aTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATaa                                                                          <  2:1561519/150‑1 (MQ=255)
                                                                               ttGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAAc                                                                         <  2:548028/150‑1 (MQ=255)
                                                                                tGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACa                                                                        <  1:1288550/150‑1 (MQ=255)
                                                                                  ttCACGGCAAAGTAGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGAAGCAACGATAACaaa                                                                      >  1:658062/1‑149 (MQ=255)
                                                                                   tCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATc                                                                     <  1:1374536/150‑1 (MQ=255)
                                                                                    cACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATcc                                                                    >  2:333608/1‑149 (MQ=255)
                                                                                      cGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc                                                                  >  1:1374982/1‑150 (MQ=255)
                                                                                      cGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTCACCGCTGCTGCCGTTCCTGGCACCATAAGCTCTGTAGCAACCATAACAATCaac                                                                  >  2:900514/1‑148 (MQ=255)
                                                                                        gCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaa                                                                >  1:1793949/1‑150 (MQ=255)
                                                                                           aaGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaacc                                                             >  2:1131980/1‑150 (MQ=255)
                                                                                            aGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCTGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaacca                                                            >  2:325606/1‑150 (MQ=255)
                                                                                              ttGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaac                                                          >  1:1314970/1‑150 (MQ=255)
                                                                                                ggCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCAGAAGCAACGATAACAATCACCAAACcaacaa                                                        >  2:1723850/1‑150 (MQ=255)
                                                                                                 gCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAg                                                       <  1:335564/150‑1 (MQ=255)
                                                                                                       cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCCACGATAACAATCACCAAACCCACAAGCACCgg                                                 >  1:152768/1‑150 (MQ=255)
                                                                                                       cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCgg                                                 <  1:1813423/150‑1 (MQ=255)
                                                                                                         ctaCGCATATACCACAGTGGGGGTCATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTa                                               <  2:877693/148‑1 (MQ=255)
                                                                                                            cGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCg                                            >  1:1051367/1‑150 (MQ=255)
                                                                                                                    ccACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGTAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGt                                    >  2:1597999/1‑150 (MQ=255)
                                                                                                                       caGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGtttt                                 >  2:1565002/1‑150 (MQ=255)
                                                                                                                        aGTTGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCTGTACCGAGGTGCGTTTTa                                <  2:1586810/150‑1 (MQ=255)
                                                                                                                         gTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTAc                               <  2:78123/150‑1 (MQ=255)
                                                                                                                          tGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACg                              >  2:23122/1‑150 (MQ=255)
                                                                                                                          tGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACg                              <  1:1150022/150‑1 (MQ=255)
                                                                                                                            ggTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGAc                            <  1:1191819/150‑1 (MQ=255)
                                                                                                                                 aatagtAATGCCGGAGGCAAGTTTCCTCCGGCTTTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCaa                       >  1:120099/6‑150 (MQ=39)
                                                                                                                                    agtAATGCCGGAGGCAAGTTTCCTCCGGCTTTACTAGTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACg                    >  1:1402390/3‑150 (MQ=255)
                                                                                                                                      tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCTACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGc                  >  1:1188483/1‑150 (MQ=255)
                                                                                                                                      tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGAAAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGc                  <  1:524099/150‑1 (MQ=255)
                                                                                                                                       aaaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGCCCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATACCAATCACCACACCAACAAGCCCCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCg                 >  1:1121444/1‑150 (MQ=255)
                                                                                                                                        atgCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACACTCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCga               >  1:278208/3‑150 (MQ=255)
                                                                                                                                        atgCCGGAGGCAAGTTTCCTCCGGCATTACAATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCga               >  2:1475756/3‑150 (MQ=255)
                                                                                                                                         tgCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCgag              >  2:1224130/2‑150 (MQ=255)
                                                                                                                                         aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCgag              <  2:579269/150‑1 (MQ=255)
                                                                                                                                         aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCgag              <  2:1435588/150‑1 (MQ=255)
                                                                                                                                         aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCgag              <  1:1443041/150‑1 (MQ=255)
                                                                                                                                          gCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTGTTACGACTTCAACCGGCgagc             >  1:1499992/1‑149 (MQ=255)
                                                                                                                                                ggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCg       <  1:746368/150‑1 (MQ=255)
                                                                                                                                                ggaAACTTGCCTCCGGCATTACTATTTACCTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCg       <  2:1643705/150‑1 (MQ=255)
                                                                                                                                                  aaaCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGcc     <  1:1389669/150‑1 (MQ=255)
                                                                                                                                                   atcTTGCCTCCGGGATTACTATTTACTTGCCTGTGACCGCTTGTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGTTACAGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCa    <  1:1224633/148‑1 (MQ=255)
                                                                                                                                                     cTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAAGATCtcgccgtt   <  1:1401952/150‑8 (MQ=255)
                                                                                                                                                     cTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATc  <  2:440560/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATC  >  NC_000913/654414‑654712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: