Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 662,684 G→T 5.3% intergenic (‑42/+68) ybeD ← / ← dacA UPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913662,6840GT 5.3% 388.5 / 9.9 132intergenic (‑42/+68)ybeD/dacAUPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base G (68/57);  new base T (5/2);  total (73/59)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.93e-01

Only 100 of 132 total aligned reads displayed.

ACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCA  >  NC_000913/662539‑662831
                                                                                                                                                 |                                                                                                                                                   
aCCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAgg                                                                                                                                                 >  2:258725/1‑150 (MQ=255)
 ccACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGc                                                                                                                                                <  1:1710580/150‑1 (MQ=255)
  cACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTGCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCg                                                                                                                                               >  1:1681346/1‑150 (MQ=255)
   aCTTCAACCACCTGATCAACCAGCTCAGGTAAAGCCTGCCCCATAACTTTGAAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAAATAAATAGCAACTCCCGCCAGAAGGCgg                                                                                                                                              >  1:1187615/1‑150 (MQ=255)
     ttCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTACCGCCAGAAGGCGGGa                                                                                                                                            >  2:132122/1‑150 (MQ=255)
      tCAACCACCTGATCAACCAGCTCAGGTAACTCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAg                                                                                                                                           <  2:342217/150‑1 (MQ=255)
      tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                                                                                                                           <  1:1517901/150‑1 (MQ=255)
        aaCCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAGtt                                                                                                                                         <  2:1012949/150‑1 (MQ=255)
         accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttt                                                                                                                                        <  1:1123896/150‑1 (MQ=255)
         accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttt                                                                                                                                        >  2:1028920/1‑150 (MQ=255)
             ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTTAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATTTCAGCTCAGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTa                                                                                                                                    >  1:1647109/1‑150 (MQ=255)
             ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTa                                                                                                                                    <  2:1561081/150‑1 (MQ=255)
              cTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTAc                                                                                                                                   <  1:1203980/150‑1 (MQ=255)
               tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGGCGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACt                                                                                                                                  <  1:1432062/150‑1 (MQ=255)
                 aTCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGa                                                                                                                                <  1:1818506/150‑1 (MQ=255)
                   cAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTAatgatg                                                                                                                              >  2:1282229/1‑150 (MQ=255)
                   cAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATg                                                                                                                              <  1:844609/150‑1 (MQ=255)
                      ccAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCtt                                                                                                                           <  1:149213/150‑1 (MQ=255)
                       cAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTa                                                                                                                          <  2:549525/150‑1 (MQ=255)
                        aGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAg                                                                                                                         <  1:16093/150‑1 (MQ=255)
                        aGCTCAGGTAACGCCTGCCCCATAACTATGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAATTAGAAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAg                                                                                                                         <  2:1179050/150‑1 (MQ=255)
                          cTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                                                                                                                       >  2:1395955/1‑150 (MQ=255)
                              ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATg                                                                                                                   >  2:237427/1‑150 (MQ=255)
                               gTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTATTTTAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTTTATgg                                                                                                                  <  2:617294/150‑1 (MQ=255)
                                 aaCGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgggg                                                                                                                >  2:322684/1‑150 (MQ=255)
                                   cGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAc                                                                                                              >  1:1031590/1‑150 (MQ=255)
                                    gCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACg                                                                                                             >  1:635444/1‑150 (MQ=255)
                                     ccTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATTGGGACgg                                                                                                            <  1:1563687/150‑1 (MQ=255)
                                           ccATAACTTTGT‑AGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTac                                                                                                     >  2:1065615/1‑150 (MQ=255)
                                            cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTac                                                                                                     >  1:243190/1‑150 (MQ=255)
                                            cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTac                                                                                                     <  2:556163/150‑1 (MQ=255)
                                                aCTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACtt                                                                                                 >  2:1358390/1‑150 (MQ=255)
                                                      taagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAgt                                                                                           >  1:954969/1‑150 (MQ=255)
                                                        agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTTAATAGCATCTCCCGCCAGCAGGCGTGAGTTTTTTACTGATGCGTAGTAGATGGGGACGGAAATTACACTTTCAAgtgg                                                                                         >  2:169398/1‑149 (MQ=255)
                                                        agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAgtgt                                                                                         >  1:821036/1‑150 (MQ=255)
                                                        agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAgtgt                                                                                         >  2:1030978/1‑150 (MQ=255)
                                                         gtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGtt                                                                                        >  2:471542/1‑150 (MQ=255)
                                                         gtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGtt                                                                                        >  2:500706/1‑150 (MQ=255)
                                                         gtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGtt                                                                                        <  2:225528/150‑1 (MQ=255)
                                                           aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTa                                                                                      >  2:1801344/1‑150 (MQ=255)
                                                              aGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAtt                                                                                   <  1:1707426/150‑1 (MQ=255)
                                                               ggtgTAGGGATTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGTAAATTACACTTTCATGTGTTTAAttt                                                                                  >  1:898243/4‑150 (MQ=255)
                                                                   tAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaa                                                                              <  1:1620445/150‑1 (MQ=255)
                                                                    aGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaac                                                                             >  2:1553498/1‑150 (MQ=255)
                                                                    aGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaac                                                                             <  1:258725/150‑1 (MQ=255)
                                                                    aGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGAAGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTATTaaa                                                                             >  2:1683912/1‑149 (MQ=255)
                                                                        aaTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaa                                                                         <  2:1294255/150‑1 (MQ=255)
                                                                          ttCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGTGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaacc                                                                       <  1:335922/150‑1 (MQ=255)
                                                                          ttCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTAAATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaacc                                                                       >  1:1702790/1‑150 (MQ=255)
                                                                              aGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTg                                                                   >  2:1510382/1‑150 (MQ=255)
                                                                              aGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTAGATGGGGACGGAAATTACCCTTTCAAGTGTTTAATTTTTAACCAAACCAGTg                                                                   >  2:1302423/1‑150 (MQ=255)
                                                                                 aGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTAAAGTGTTTAATTTTTAAACAAACCAGTGATg                                                                >  2:1070375/1‑150 (MQ=255)
                                                                                  gTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATgg                                                               <  2:1643482/150‑1 (MQ=255)
                                                                                  gTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATgg                                                               <  2:1648958/150‑1 (MQ=255)
                                                                                   ttCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGa                                                              <  1:910190/150‑1 (MQ=255)
                                                                                    tCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGATTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGaa                                                             >  1:1411002/1‑150 (MQ=255)
                                                                                     cGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAAGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAAc                                                            >  2:1168572/1‑150 (MQ=255)
                                                                                        tAAGTTTGGTTTTCATGTCAGCTCCTGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCATAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAtt                                                         <  1:1455422/150‑1 (MQ=255)
                                                                                         aaGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAtta                                                        >  2:516717/1‑150 (MQ=255)
                                                                                               ggTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaa                                                  <  2:1758929/150‑1 (MQ=255)
                                                                                                  tttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGt                                               <  2:230146/150‑1 (MQ=255)
                                                                                                  tttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGt                                               >  2:528919/1‑150 (MQ=255)
                                                                                                   ttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTa                                              >  2:441480/1‑150 (MQ=255)
                                                                                                         tCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAAt                                        >  2:126540/1‑150 (MQ=255)
                                                                                                         tCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAAt                                        <  1:1297527/150‑1 (MQ=255)
                                                                                                            gCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAt                                     >  2:580550/1‑150 (MQ=255)
                                                                                                               ccGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAtttt                                  <  2:1610505/150‑1 (MQ=255)
                                                                                                                ctgCGTAACGTAATTAAAAAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTg                                 <  2:1316073/148‑1 (MQ=255)
                                                                                                                  gCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGcc                               >  1:1738960/1‑150 (MQ=255)
                                                                                                                    gtaacgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCga                             <  1:34877/150‑1 (MQ=255)
                                                                                                                    gtaacgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCga                             >  2:908689/1‑150 (MQ=255)
                                                                                                                      aacgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaag                           <  2:1324635/150‑1 (MQ=255)
                                                                                                                          taaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGt                       <  1:569112/150‑1 (MQ=255)
                                                                                                                          taaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGGATATGGGGACGTAAATTTCACTTTCTAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGt                       <  2:966364/150‑1 (MQ=255)
                                                                                                                           aaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATTTAATCAATGATTTTGCCGAAGAAGtt                      >  2:1149710/1‑150 (MQ=255)
                                                                                                                           aaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGtt                      >  2:292827/1‑150 (MQ=255)
                                                                                                                              tAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTAcc                   >  1:497328/1‑150 (MQ=255)
                                                                                                                                 aTAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGCCGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCTATGATTTTGCCGAAGCAGTTACCTTc                >  1:735916/1‑150 (MQ=255)
                                                                                                                                 aTAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTc                >  1:667962/1‑150 (MQ=255)
                                                                                                                                  aaaaaaCTCCCGCCTTCTGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTcc               >  2:359042/5‑150 (MQ=255)
                                                                                                                                   aaaaaCTCCCGCCTTCTGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCg              >  2:19396/4‑150 (MQ=255)
                                                                                                                                   aaaaaCTCCCGCCTTCTGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCg              >  2:739806/4‑150 (MQ=255)
                                                                                                                                    gCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCTAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCgg             >  1:1573306/1‑150 (MQ=255)
                                                                                                                                    gCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCgg             >  2:1658209/1‑150 (MQ=255)
                                                                                                                                     cAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCTAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCggg            <  2:243190/150‑1 (MQ=255)
                                                                                                                                       aCTCCCGCCTTCTGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAt          >  1:952233/1‑150 (MQ=255)
                                                                                                                                       aCTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAt          <  2:1388279/150‑1 (MQ=255)
                                                                                                                                        cTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAtt         >  1:431586/1‑150 (MQ=255)
                                                                                                                                        cTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAtt         >  1:1200483/1‑150 (MQ=255)
                                                                                                                                         tCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACAAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAttt        >  1:1085408/1‑150 (MQ=255)
                                                                                                                                          cccGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCTGGATTTc       >  1:1111387/1‑150 (MQ=255)
                                                                                                                                          cccGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTc       >  2:1519899/1‑150 (MQ=255)
                                                                                                                                           ccGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGTCGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTATTGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCt      >  1:353264/1‑150 (MQ=255)
                                                                                                                                            cGCCAGAAGTCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCtt     <  1:1282229/150‑1 (MQ=255)
                                                                                                                                            cGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCtt     >  2:399569/1‑150 (MQ=255)
                                                                                                                                            cGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCtt     >  2:899353/1‑150 (MQ=255)
                                                                                                                                             gCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTg    >  2:1044348/1‑150 (MQ=255)
                                                                                                                                              ccAGAAGGCGGGAGTTTTTTACTGATGCTTTGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGc   <  1:132122/150‑1 (MQ=255)
                                                                                                                                              ccAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTTATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGTTTTCTTGc   <  2:1489762/150‑1 (MQ=255)
                                                                                                                                              ccAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGc   <  1:1540127/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCA  >  NC_000913/662539‑662831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑