Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 691,858 T→G 6.2% intergenic (‑74/+16) ybeX ← / ← ybeY putative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913691,8580TG 6.2% 327.3 / 9.3 113intergenic (‑74/+16)ybeX/ybeYputative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor
Reads supporting (aligned to +/- strand):  ref base T (58/48);  new base G (3/4);  total (61/52)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

Only 100 of 114 total aligned reads displayed.

GTTGGCTGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCACC  >  NC_000913/691709‑692006
                                                                                                                                                     |                                                                                                                                                    
gTTGGCTGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGt                                                                                                                                                      >  1:675626/1‑150 (MQ=255)
     cTGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAAt                                                                                                                                                 >  2:1260369/1‑150 (MQ=255)
         gTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCt                                                                                                                                             <  2:889580/150‑1 (MQ=255)
          tAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTg                                                                                                                                            <  2:1775198/150‑1 (MQ=255)
           aaCAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTgg                                                                                                                                           >  1:612725/1‑150 (MQ=255)
            aCAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGc                                                                                                                                          >  1:1689413/1‑150 (MQ=255)
             cAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCa                                                                                                                                         >  1:810201/1‑150 (MQ=255)
              aGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTTATCACCAACGGCGGGGACGTATGCCAGTCAAATGCCTGGCaa                                                                                                                                        >  2:1624145/1‑150 (MQ=255)
               gggAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCaaa                                                                                                                                       >  1:884375/1‑150 (MQ=255)
               gggAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCaga                                                                                                                                       <  1:196360/150‑3 (MQ=255)
                 gagaAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGATTTGTCGTCGATCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGAAAatt                                                                                                                                     <  2:402304/150‑1 (MQ=255)
                 gagaAAAATCCCTGCTTGTTGCTTATCGTGTCACTACTGAGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCagacg                                                                                                                                     <  1:1662082/150‑5 (MQ=255)
                   taaaaaTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCagacgtc                                                                                                                                   <  2:551657/149‑7 (MQ=255)
                       aaTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt                                                                                                                               <  2:1310008/150‑1 (MQ=255)
                        aTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCTTCGTATGGGTTCTCATGTTAGTTTATCAAAACGCCGTCGTTAATCACCAACGGCTGGGACGTCTGCAAGTCAAATGCCTTGCAAATTAttattt                                                                                                                              >  2:1682372/1‑150 (MQ=255)
                           ccTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATAACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAATCTTtctc                                                                                                                           >  1:1479917/1‑150 (MQ=255)
                             ttcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCgg                                                                                                                         >  2:573362/1‑150 (MQ=255)
                              tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                        <  1:581943/150‑1 (MQ=255)
                              tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                        <  1:533872/150‑1 (MQ=255)
                              tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                        >  2:1151907/1‑150 (MQ=255)
                              tcttGTTGCTTATCGTGTCACTACTGAGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATTTTAGTTAATCAAAACGCCGTCGTTAAACACCAACGTCGGGGACGTCTGCCAGTCAAATGCATAGAAAATTATTATATCTCTGc                                                                                                                        >  2:1151909/1‑150 (MQ=255)
                                 tgttgCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAt                                                                                                                     >  1:447121/1‑150 (MQ=255)
                                     gCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTAc                                                                                                                 >  1:735652/1‑150 (MQ=255)
                                      cTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACg                                                                                                                <  2:675626/150‑1 (MQ=255)
                                           ggtgtCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATcc                                                                                                           <  1:1681215/149‑1 (MQ=255)
                                            gtttCACTACTGTGTGAATTGTCGTGGCTCATGGCGTCGTATGGGTTCTCATGTAAGTTAATCAAAACGCCGTCGTTAATCTCCTACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCt                                                                                                          <  2:468892/147‑1 (MQ=255)
                                            gtgtCACTACTGTGTGTATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAAGTACGGATCCt                                                                                                          <  1:1340201/150‑1 (MQ=255)
                                            gtgtCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCt                                                                                                          <  1:1823596/150‑1 (MQ=255)
                                                 actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAg                                                                                                     >  1:1469262/1‑150 (MQ=255)
                                                 actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAAAGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAg                                                                                                     <  2:1093263/150‑1 (MQ=255)
                                                  ctactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGc                                                                                                    >  2:468781/1‑150 (MQ=255)
                                                    actGTGTGAATTGTCGTCGCTCATGGGGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc                                                                                                  <  2:381909/150‑1 (MQ=255)
                                                    actGTGTGAATTGTCGTCGCTCATGGCGTCGATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCCGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATATCCCa                                                                                                 >  1:102471/1‑150 (MQ=255)
                                                      tgtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCag                                                                                                >  1:165384/1‑150 (MQ=255)
                                                       gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCaga                                                                                               >  1:848507/1‑150 (MQ=255)
                                                       gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCaga                                                                                               >  2:1662089/1‑150 (MQ=255)
                                                         gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagc                                                                                             >  1:1777857/1‑150 (MQ=255)
                                                          tgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagca                                                                                            >  2:477918/1‑150 (MQ=255)
                                                          tgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagca                                                                                            <  2:936608/150‑1 (MQ=255)
                                                           gAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaa                                                                                           >  2:917156/1‑150 (MQ=255)
                                                            aaTTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaag                                                                                          >  1:1195423/1‑150 (MQ=255)
                                                              ttGTCGGCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAACACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaagca                                                                                        <  2:1218742/150‑1 (MQ=255)
                                                               tGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCAt                                                                                       >  2:1093334/1‑150 (MQ=255)
                                                                gtcgtcGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATa                                                                                      >  2:1141630/1‑150 (MQ=255)
                                                                      gCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATctct                                                                                >  1:1016394/1‑150 (MQ=255)
                                                                       cTCATGGCGTCGTATGGGTTGTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                               <  2:891579/150‑1 (MQ=255)
                                                                       cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                               >  1:346166/1‑150 (MQ=255)
                                                                       cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGGCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                               <  2:891582/150‑1 (MQ=255)
                                                                       cTCATGGCGTCGTATGGGTGCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                               <  1:1593740/150‑1 (MQ=255)
                                                                         cATGGCGTCGTATGGGTTCTCAAGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCCGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGtt                                                                             >  2:1138301/1‑150 (MQ=255)
                                                                             gCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCTCCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGa                                                                         <  1:1322847/150‑1 (MQ=255)
                                                                              cgtcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGTGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAg                                                                        <  1:1825560/150‑1 (MQ=255)
                                                                                tcgtATGGGTTCTCATGTGAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAggg                                                                      <  1:974669/150‑1 (MQ=255)
                                                                                  gaaTGGGTTCACATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACCGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGAACGGATCCTCATAGCCCAGAGCAAGCATAATCTCAGGTACGAGGGCt                                                                    >  2:1041971/3‑150 (MQ=255)
                                                                                     tGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTcc                                                                 <  2:667319/150‑1 (MQ=255)
                                                                                      gggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCa                                                                >  2:1397042/1‑150 (MQ=255)
                                                                                       ggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCAt                                                               >  2:1505592/1‑150 (MQ=255)
                                                                                       ggTGCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGAAAATTATTCTTTCTCGGCAATTTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCAt                                                               <  2:1281794/150‑1 (MQ=255)
                                                                                        gTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCAtt                                                              >  1:977246/1‑150 (MQ=255)
                                                                                          tctcATGTTAGTTAATGAAAACGCCGTCGTTATTCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGGATATTTTTCTTTCTCGGCAATGTACGGATCATCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttc                                                            <  1:349074/150‑1 (MQ=255)
                                                                                            tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttctt                                                          <  1:673381/150‑1 (MQ=255)
                                                                                            tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttctt                                                          >  2:659986/1‑150 (MQ=255)
                                                                                              aTGTTAGTTAATCAAAACGCCTTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTtcttct                                                        <  1:243919/150‑1 (MQ=255)
                                                                                              aTGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTtcttct                                                        <  2:629876/150‑1 (MQ=255)
                                                                                               tgtttgttAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTg                                                       <  1:824328/150‑1 (MQ=255)
                                                                                                   agttaATCAAAACGCGGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTc                                                   <  1:134955/150‑1 (MQ=255)
                                                                                                   agttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTc                                                   <  1:718088/150‑1 (MQ=255)
                                                                                                    gttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTGTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCg                                                  <  2:270384/150‑1 (MQ=255)
                                                                                                    gttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCg                                                  >  2:1492022/1‑150 (MQ=255)
                                                                                                       aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCa                                               <  2:624627/150‑1 (MQ=255)
                                                                                                       aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCa                                               >  1:157364/1‑150 (MQ=255)
                                                                                                           aaaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAAATATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTATGCTTCGTCATCtt                                           >  1:662949/1‑150 (MQ=255)
                                                                                                            aaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTc                                          <  1:607829/150‑1 (MQ=255)
                                                                                                              aCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTCTTCTGCTTCGTCATCTTCGAt                                       <  2:612725/150‑1 (MQ=255)
                                                                                                               cGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGAt                                       >  1:529717/1‑150 (MQ=255)
                                                                                                               cGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGAt                                       >  2:25049/1‑150 (MQ=255)
                                                                                                                      gtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCg                                <  2:373321/150‑1 (MQ=255)
                                                                                                                        tAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCCATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTGTCGATGGGTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGtc                              >  1:294645/1‑149 (MQ=255)
                                                                                                                              ccAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                        >  2:1106184/1‑150 (MQ=255)
                                                                                                                              ccAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                        >  1:1242325/1‑150 (MQ=255)
                                                                                                                              ccAACGGCGGGGACGTCAGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATACTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                        >  1:1656080/1‑150 (MQ=255)
                                                                                                                               cAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAAGTACGGATCCTCATAGCCCAGCGCAAGCATAATCTCTGTTTCGAGCGCTTCCATGTCGTGTGCTTCGTCATCTACGATGTGATCGTAACCTaaa                       >  2:343047/1‑149 (MQ=255)
                                                                                                                                aaCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACa                      <  2:346166/150‑1 (MQ=255)
                                                                                                                                 aCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACaa                     <  1:1151907/150‑1 (MQ=255)
                                                                                                                                    gcggGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATg                  <  2:1195423/150‑1 (MQ=255)
                                                                                                                                     gggggACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGc                 <  1:1760855/149‑1 (MQ=255)
                                                                                                                                     cggGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGc                 <  2:165384/150‑1 (MQ=255)
                                                                                                                                           cGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTg           >  2:21159/1‑150 (MQ=255)
                                                                                                                                            gTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGc          <  2:810201/150‑1 (MQ=255)
                                                                                                                                            atttGCCAGGCATTTGACTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGc          >  1:416295/4‑150 (MQ=255)
                                                                                                                                            atttGCCAGGCATTTGACTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGATTCGAGCGATTCCATTTCTTCTGCTTCGACATCATCGATGTGATCGTAACCTAACAAATCCAGACTGc          >  2:180667/4‑150 (MQ=255)
                                                                                                                                             tCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCATCTGCATCGTCATCTTCGAAGTGATCGTAACCTAACAACTGCAGACTGcc         >  2:925951/1‑150 (MQ=255)
                                                                                                                                               tGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGt       >  1:1343680/1‑150 (MQ=255)
                                                                                                                                               tGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGACCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGt       >  1:1223090/1‑150 (MQ=255)
                                                                                                                                               tGCCAGTCAAATGCCTGGCAAATTATTCGTTCTCGGCAATGTACGGATCCTCATATCCAAGAGCAAGCATAATCTCTGTTTCGAGGGATTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACCAATGAAGACGGCCgg       >  1:1057181/1‑149 (MQ=255)
                                                                                                                                                gCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTg      <  1:1656128/150‑1 (MQ=255)
                                                                                                                                                gCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTg      <  2:1302654/150‑1 (MQ=255)
                                                                                                                                                  cAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGca    >  1:1151231/1‑150 (MQ=255)
                                                                                                                                                  cAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGca    >  2:1492939/1‑150 (MQ=255)
                                                                                                                                                    gTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGcacc  >  1:493596/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTGGCTGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCACC  >  NC_000913/691709‑692006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: