Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 691,861 A→T 6.1% intergenic (‑77/+13) ybeX ← / ← ybeY putative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913691,8610AT 6.1% 336.3 / 6.1 112intergenic (‑77/+13)ybeX/ybeYputative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor
Reads supporting (aligned to +/- strand):  ref base A (57/48);  new base T (3/4);  total (60/52)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 114 total aligned reads displayed.

GGCTGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCACCACCA  >  NC_000913/691712‑692010
                                                                                                                                                     |                                                                                                                                                     
  cTGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAAt                                                                                                                                                     >  2:1260369/1‑150 (MQ=255)
      gTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCt                                                                                                                                                 <  2:889580/150‑1 (MQ=255)
        aaCAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTgg                                                                                                                                               >  1:612725/1‑150 (MQ=255)
         aCAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGc                                                                                                                                              >  1:1689413/1‑150 (MQ=255)
          cAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCa                                                                                                                                             >  1:810201/1‑150 (MQ=255)
           aGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTTATCACCAACGGCGGGGACGTATGCCAGTCAAATGCCTGGCaa                                                                                                                                            >  2:1624145/1‑150 (MQ=255)
            gggAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCaaa                                                                                                                                           >  1:884375/1‑150 (MQ=255)
            gggAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCaga                                                                                                                                           <  1:196360/150‑3 (MQ=255)
              gagaAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGATTTGTCGTCGATCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGAAAatt                                                                                                                                         <  2:402304/150‑1 (MQ=255)
              gagaAAAATCCCTGCTTGTTGCTTATCGTGTCACTACTGAGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCagacg                                                                                                                                         <  1:1662082/150‑5 (MQ=255)
               agaAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAatta                                                                                                                                        >  2:103106/1‑150 (MQ=255)
                taaaaaTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCagacgtc                                                                                                                                       <  2:551657/149‑7 (MQ=255)
                    aaTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt                                                                                                                                   <  2:1310008/150‑1 (MQ=255)
                     aTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCTTCGTATGGGTTCTCATGTTAGTTTATCAAAACGCCGTCGTTAATCACCAACGGCTGGGACGTCTGCAAGTCAAATGCCTTGCAAATTAttattt                                                                                                                                  >  2:1682372/1‑150 (MQ=255)
                        ccTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATAACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAATCTTtctc                                                                                                                               >  1:1479917/1‑150 (MQ=255)
                          ttcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCgg                                                                                                                             >  2:573362/1‑150 (MQ=255)
                           tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                            <  1:533872/150‑1 (MQ=255)
                           tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                            >  1:730593/1‑150 (MQ=255)
                           tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                            >  2:1151907/1‑150 (MQ=255)
                           tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                            <  1:581943/150‑1 (MQ=255)
                           tcttGTTGCTTATCGTGTCACTACTGAGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATTTTAGTTAATCAAAACGCCGTCGTTAAACACCAACGTCGGGGACGTCTGCCAGTCAAATGCATAGAAAATTATTATATCTCTGc                                                                                                                            >  2:1151909/1‑150 (MQ=255)
                              tgttgCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAt                                                                                                                         >  1:447121/1‑150 (MQ=255)
                                   cTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACg                                                                                                                    <  2:675626/150‑1 (MQ=255)
                                      aTCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGAt                                                                                                                 >  2:1705737/1‑150 (MQ=255)
                                       tCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc                                                                                                                >  2:1442534/1‑150 (MQ=255)
                                        ggtgtCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATcc                                                                                                               <  1:1681215/149‑1 (MQ=255)
                                         gtttCACTACTGTGTGAATTGTCGTGGCTCATGGCGTCGTATGGGTTCTCATGTAAGTTAATCAAAACGCCGTCGTTAATCTCCTACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCt                                                                                                              <  2:468892/147‑1 (MQ=255)
                                         gtgtCACTACTGTGTGTATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAAGTACGGATCCt                                                                                                              <  1:1340201/150‑1 (MQ=255)
                                         gtgtCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCt                                                                                                              <  1:1823596/150‑1 (MQ=255)
                                           gtCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCa                                                                                                            <  2:1173349/150‑1 (MQ=255)
                                              actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAg                                                                                                         >  1:1469262/1‑150 (MQ=255)
                                              actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAAAGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAg                                                                                                         <  2:1093263/150‑1 (MQ=255)
                                               ctactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGc                                                                                                        >  2:468781/1‑150 (MQ=255)
                                                 actGTGTGAATTGTCGTCGCTCATGGGGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc                                                                                                      <  2:381909/150‑1 (MQ=255)
                                                 actGTGTGAATTGTCGTCGCTCATGGCGTCGATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCCGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATATCCCa                                                                                                     >  1:102471/1‑150 (MQ=255)
                                                   tgtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCag                                                                                                    >  1:165384/1‑150 (MQ=255)
                                                    gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCaga                                                                                                   >  2:1662089/1‑150 (MQ=255)
                                                    gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCaga                                                                                                   >  1:848507/1‑150 (MQ=255)
                                                      gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagc                                                                                                 >  1:1777857/1‑150 (MQ=255)
                                                       tgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagca                                                                                                <  2:936608/150‑1 (MQ=255)
                                                       tgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagca                                                                                                >  2:477918/1‑150 (MQ=255)
                                                        gAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaa                                                                                               >  2:917156/1‑150 (MQ=255)
                                                         aaTTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaag                                                                                              >  1:1195423/1‑150 (MQ=255)
                                                           ttGTCGGCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAACACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaagca                                                                                            <  2:1218742/150‑1 (MQ=255)
                                                            tGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCAt                                                                                           >  2:1093334/1‑150 (MQ=255)
                                                             gtcgtcGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATa                                                                                          >  2:1141630/1‑150 (MQ=255)
                                                                   gCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATctct                                                                                    >  1:1016394/1‑150 (MQ=255)
                                                                    cTCATGGCGTCGTATGGGTTGTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                                   <  2:891579/150‑1 (MQ=255)
                                                                    cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                                   >  1:346166/1‑150 (MQ=255)
                                                                    cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGGCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                                   <  2:891582/150‑1 (MQ=255)
                                                                    cTCATGGCGTCGTATGGGTGCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                                   <  1:1593740/150‑1 (MQ=255)
                                                                      cATGGCGTCGTATGGGTTCTCAAGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCCGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGtt                                                                                 >  2:1138301/1‑150 (MQ=255)
                                                                          gCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCTCCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGa                                                                             <  1:1322847/150‑1 (MQ=255)
                                                                           cgtcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGTGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAg                                                                            <  1:1825560/150‑1 (MQ=255)
                                                                             tcgtATGGGTTCTCATGTGAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAggg                                                                          <  1:974669/150‑1 (MQ=255)
                                                                                  tGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTcc                                                                     <  2:667319/150‑1 (MQ=255)
                                                                                   gggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCa                                                                    >  2:1397042/1‑150 (MQ=255)
                                                                                    ggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCAt                                                                   >  2:1505592/1‑150 (MQ=255)
                                                                                    ggTGCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGAAAATTATTCTTTCTCGGCAATTTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCAt                                                                   <  2:1281794/150‑1 (MQ=255)
                                                                                     gTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCAtt                                                                  >  1:977246/1‑150 (MQ=255)
                                                                                       tctcATGTTAGTTAATGAAAACGCCGTCGTTATTCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGGATATTTTTCTTTCTCGGCAATGTACGGATCATCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttc                                                                <  1:349074/150‑1 (MQ=255)
                                                                                         tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttctt                                                              >  2:659986/1‑150 (MQ=255)
                                                                                         tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttctt                                                              <  1:673381/150‑1 (MQ=255)
                                                                                           aTGTTAGTTAATCAAAACGCCTTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTtcttct                                                            <  1:243919/150‑1 (MQ=255)
                                                                                           aTGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTtcttct                                                            <  2:629876/150‑1 (MQ=255)
                                                                                            tgtttgttAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTg                                                           <  1:824328/150‑1 (MQ=255)
                                                                                                agttaATCAAAACGCGGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTc                                                       <  1:134955/150‑1 (MQ=255)
                                                                                                agttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTc                                                       <  1:718088/150‑1 (MQ=255)
                                                                                                 gttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTGTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCg                                                      <  2:270384/150‑1 (MQ=255)
                                                                                                    aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCa                                                   <  2:624627/150‑1 (MQ=255)
                                                                                                    aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCa                                                   >  1:157364/1‑150 (MQ=255)
                                                                                                        aaaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAAATATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTATGCTTCGTCATCtt                                               >  1:662949/1‑150 (MQ=255)
                                                                                                         aaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTc                                              <  1:607829/150‑1 (MQ=255)
                                                                                                           aCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTCTTCTGCTTCGTCATCTTCGAt                                           <  2:612725/150‑1 (MQ=255)
                                                                                                            cGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGAt                                           >  2:25049/1‑150 (MQ=255)
                                                                                                            cGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGAt                                           >  1:529717/1‑150 (MQ=255)
                                                                                                                   gtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCg                                    <  2:373321/150‑1 (MQ=255)
                                                                                                                     tAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCCATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTGTCGATGGGTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGtc                                  >  1:294645/1‑149 (MQ=255)
                                                                                                                           ccAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                            >  1:1242325/1‑150 (MQ=255)
                                                                                                                           ccAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                            >  2:1106184/1‑150 (MQ=255)
                                                                                                                           ccAACGGCGGGGACGTCAGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATACTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                            >  1:1656080/1‑150 (MQ=255)
                                                                                                                            cAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAAGTACGGATCCTCATAGCCCAGCGCAAGCATAATCTCTGTTTCGAGCGCTTCCATGTCGTGTGCTTCGTCATCTACGATGTGATCGTAACCTaaa                           >  2:343047/1‑149 (MQ=255)
                                                                                                                             aaCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACa                          <  2:346166/150‑1 (MQ=255)
                                                                                                                              aCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACaa                         <  1:1151907/150‑1 (MQ=255)
                                                                                                                                  gggggACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGc                     <  1:1760855/149‑1 (MQ=255)
                                                                                                                                  cggGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGc                     <  2:165384/150‑1 (MQ=255)
                                                                                                                                        cGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTg               >  2:21159/1‑150 (MQ=255)
                                                                                                                                         gTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGc              <  2:810201/150‑1 (MQ=255)
                                                                                                                                         atttGCCAGGCATTTGACTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGc              >  1:416295/4‑150 (MQ=255)
                                                                                                                                         atttGCCAGGCATTTGACTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGATTCGAGCGATTCCATTTCTTCTGCTTCGACATCATCGATGTGATCGTAACCTAACAAATCCAGACTGc              >  2:180667/4‑150 (MQ=255)
                                                                                                                                          tCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCATCTGCATCGTCATCTTCGAAGTGATCGTAACCTAACAACTGCAGACTGcc             >  2:925951/1‑150 (MQ=255)
                                                                                                                                            tGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGt           >  1:1343680/1‑150 (MQ=255)
                                                                                                                                            tGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGACCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGt           >  1:1223090/1‑150 (MQ=255)
                                                                                                                                            tGCCAGTCAAATGCCTGGCAAATTATTCGTTCTCGGCAATGTACGGATCCTCATATCCAAGAGCAAGCATAATCTCTGTTTCGAGGGATTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACCAATGAAGACGGCCgg           >  1:1057181/1‑149 (MQ=255)
                                                                                                                                             gCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTg          <  1:1656128/150‑1 (MQ=255)
                                                                                                                                             gCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTg          <  2:1302654/150‑1 (MQ=255)
                                                                                                                                               cAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGca        >  1:1151231/1‑150 (MQ=255)
                                                                                                                                               cAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGca        >  2:1492939/1‑150 (MQ=255)
                                                                                                                                                 gTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGcacc      >  1:493596/1‑150 (MQ=255)
                                                                                                                                                     aaTGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCAccacca  <  2:1777857/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCTGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCACCACCA  >  NC_000913/691712‑692010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: