Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 691,865 C→A 5.8% intergenic (‑81/+9) ybeX ← / ← ybeY putative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913691,8650CA 5.8% 313.9 / 8.2 113intergenic (‑81/+9)ybeX/ybeYputative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor
Reads supporting (aligned to +/- strand):  ref base C (56/50);  new base A (3/4);  total (59/54)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

Only 100 of 113 total aligned reads displayed.

GTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCACCACCATAT  >  NC_000913/691718‑692013
                                                                                                                                                   |                                                                                                                                                    
gTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCt                                                                                                                                                    <  2:889580/150‑1 (MQ=255)
 tAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTg                                                                                                                                                   <  2:1775198/150‑1 (MQ=255)
  aaCAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTgg                                                                                                                                                  >  1:612725/1‑150 (MQ=255)
   aCAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGc                                                                                                                                                 >  1:1689413/1‑150 (MQ=255)
    cAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCa                                                                                                                                                >  1:810201/1‑150 (MQ=255)
     aGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTTATCACCAACGGCGGGGACGTATGCCAGTCAAATGCCTGGCaa                                                                                                                                               >  2:1624145/1‑150 (MQ=255)
      gggAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCaaa                                                                                                                                              >  1:884375/1‑150 (MQ=255)
      gggAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCaga                                                                                                                                              <  1:196360/150‑3 (MQ=255)
        gagaAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGATTTGTCGTCGATCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGAAAatt                                                                                                                                            <  2:402304/150‑1 (MQ=255)
        gagaAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCagacg                                                                                                                                            <  2:356362/150‑5 (MQ=255)
        gagaAAAATCCCTGCTTGTTGCTTATCGTGTCACTACTGAGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCagacg                                                                                                                                            <  1:1662082/150‑5 (MQ=255)
         agaAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAatta                                                                                                                                           >  2:103106/1‑150 (MQ=255)
              aaTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt                                                                                                                                      <  2:1310008/150‑1 (MQ=255)
               aTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCTTCGTATGGGTTCTCATGTTAGTTTATCAAAACGCCGTCGTTAATCACCAACGGCTGGGACGTCTGCAAGTCAAATGCCTTGCAAATTAttattt                                                                                                                                     >  2:1682372/1‑150 (MQ=255)
                  ccTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATAACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAATCTTtctc                                                                                                                                  >  1:1479917/1‑150 (MQ=255)
                    ttcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCgg                                                                                                                                >  2:573362/1‑150 (MQ=255)
                     tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                               <  1:533872/150‑1 (MQ=255)
                     tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                               <  1:581943/150‑1 (MQ=255)
                     tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                               >  1:730593/1‑150 (MQ=255)
                     tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc                                                                                                                               >  2:1151907/1‑150 (MQ=255)
                     tcttGTTGCTTATCGTGTCACTACTGAGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATTTTAGTTAATCAAAACGCCGTCGTTAAACACCAACGTCGGGGACGTCTGCCAGTCAAATGCATAGAAAATTATTATATCTCTGc                                                                                                                               >  2:1151909/1‑150 (MQ=255)
                        tgttgCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAt                                                                                                                            >  1:447121/1‑150 (MQ=255)
                            gCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTAc                                                                                                                        >  1:735652/1‑150 (MQ=255)
                             cTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACg                                                                                                                       <  2:675626/150‑1 (MQ=255)
                                 tCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc                                                                                                                   >  2:1442534/1‑150 (MQ=255)
                                  ggtgtCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATcc                                                                                                                  <  1:1681215/149‑1 (MQ=255)
                                   gtttCACTACTGTGTGAATTGTCGTGGCTCATGGCGTCGTATGGGTTCTCATGTAAGTTAATCAAAACGCCGTCGTTAATCTCCTACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCt                                                                                                                 <  2:468892/147‑1 (MQ=255)
                                   gtgtCACTACTGTGTGTATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAAGTACGGATCCt                                                                                                                 <  1:1340201/150‑1 (MQ=255)
                                     gtCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCa                                                                                                               <  2:1173349/150‑1 (MQ=255)
                                       cACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAAAGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATa                                                                                                             >  2:556052/1‑150 (MQ=255)
                                        actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAg                                                                                                            >  1:1469262/1‑150 (MQ=255)
                                        actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAAAGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAg                                                                                                            <  2:1093263/150‑1 (MQ=255)
                                         ctactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGc                                                                                                           >  2:468781/1‑150 (MQ=255)
                                           actGTGTGAATTGTCGTCGCTCATGGGGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc                                                                                                         <  2:381909/150‑1 (MQ=255)
                                           actGTGTGAATTGTCGTCGCTCATGGCGTCGATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCCGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATATCCCa                                                                                                        >  1:102471/1‑150 (MQ=255)
                                             tgtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCag                                                                                                       >  1:165384/1‑150 (MQ=255)
                                              gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCaga                                                                                                      >  2:1662089/1‑150 (MQ=255)
                                              gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCaga                                                                                                      >  1:848507/1‑150 (MQ=255)
                                                gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagc                                                                                                    >  1:1777857/1‑150 (MQ=255)
                                                 tgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagca                                                                                                   <  2:936608/150‑1 (MQ=255)
                                                 tgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagca                                                                                                   >  2:477918/1‑150 (MQ=255)
                                                  gAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaa                                                                                                  >  2:917156/1‑150 (MQ=255)
                                                   aaTTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaag                                                                                                 >  1:1195423/1‑150 (MQ=255)
                                                     ttGTCGGCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAACACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaagca                                                                                               <  2:1218742/150‑1 (MQ=255)
                                                      tGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCAt                                                                                              >  2:1093334/1‑150 (MQ=255)
                                                             gCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATctct                                                                                       >  1:1016394/1‑150 (MQ=255)
                                                              cTCATGGCGTCGTATGGGTTGTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                                      <  2:891579/150‑1 (MQ=255)
                                                              cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                                      >  1:346166/1‑150 (MQ=255)
                                                              cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGGCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                                      <  2:891582/150‑1 (MQ=255)
                                                                cATGGCGTCGTATGGGTTCTCAAGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCCGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGtt                                                                                    >  2:1138301/1‑150 (MQ=255)
                                                                    gCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCTCCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGa                                                                                <  1:1322847/150‑1 (MQ=255)
                                                                     cgtcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGTGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAg                                                                               <  1:1825560/150‑1 (MQ=255)
                                                                         gaaTGGGTTCACATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACCGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGAACGGATCCTCATAGCCCAGAGCAAGCATAATCTCAGGTACGAGGGCt                                                                           >  2:1041971/3‑150 (MQ=255)
                                                                            tGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTcc                                                                        <  2:667319/150‑1 (MQ=255)
                                                                              ggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCAt                                                                      >  2:1505592/1‑150 (MQ=255)
                                                                              ggTGCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGAAAATTATTCTTTCTCGGCAATTTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCAt                                                                      <  2:1281794/150‑1 (MQ=255)
                                                                               gTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCAtt                                                                     >  1:977246/1‑150 (MQ=255)
                                                                                 tctcATGTTAGTTAATGAAAACGCCGTCGTTATTCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGGATATTTTTCTTTCTCGGCAATGTACGGATCATCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttc                                                                   <  1:349074/150‑1 (MQ=255)
                                                                                   tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttctt                                                                 <  1:673381/150‑1 (MQ=255)
                                                                                   tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttctt                                                                 >  2:659986/1‑150 (MQ=255)
                                                                                    cATGTTATTTAATCAAAACGCCGTCGTAAATCACCATCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGTCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttcttc                                                                <  1:341805/150‑1 (MQ=255)
                                                                                    cATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttcttc                                                                <  1:570128/150‑1 (MQ=255)
                                                                                     aTGTTAGTTAATCAAAACGCCTTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTtcttct                                                               <  1:243919/150‑1 (MQ=255)
                                                                                     aTGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTtcttct                                                               <  2:629876/150‑1 (MQ=255)
                                                                                      tgtttgttAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTg                                                              <  1:824328/150‑1 (MQ=255)
                                                                                          agttaATCAAAACGCGGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTc                                                          <  1:134955/150‑1 (MQ=255)
                                                                                          agttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTc                                                          <  1:718088/150‑1 (MQ=255)
                                                                                           gttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTGTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCg                                                         <  2:270384/150‑1 (MQ=255)
                                                                                           gttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCg                                                         >  2:1492022/1‑150 (MQ=255)
                                                                                              aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCa                                                      <  2:624627/150‑1 (MQ=255)
                                                                                              aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCa                                                      >  1:157364/1‑150 (MQ=255)
                                                                                                   aaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTc                                                 <  1:607829/150‑1 (MQ=255)
                                                                                                     aCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTCTTCTGCTTCGTCATCTTCGAt                                              <  2:612725/150‑1 (MQ=255)
                                                                                                      cGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGAt                                              >  1:529717/1‑150 (MQ=255)
                                                                                                      cGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGAt                                              >  2:25049/1‑150 (MQ=255)
                                                                                                             gtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCg                                       <  2:373321/150‑1 (MQ=255)
                                                                                                             gtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCg                                       <  1:1574142/150‑1 (MQ=255)
                                                                                                               tAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCCATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTGTCGATGGGTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGtc                                     >  1:294645/1‑149 (MQ=255)
                                                                                                                     ccAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                               >  2:1106184/1‑150 (MQ=255)
                                                                                                                     ccAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                               >  1:1242325/1‑150 (MQ=255)
                                                                                                                     ccAACGGCGGGGACGTCAGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATACTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                               >  1:1656080/1‑150 (MQ=255)
                                                                                                                      cAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAAGTACGGATCCTCATAGCCCAGCGCAAGCATAATCTCTGTTTCGAGCGCTTCCATGTCGTGTGCTTCGTCATCTACGATGTGATCGTAACCTaaa                              >  2:343047/1‑149 (MQ=255)
                                                                                                                        aCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACaa                            <  1:1151907/150‑1 (MQ=255)
                                                                                                                           gcggGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATg                         <  2:1195423/150‑1 (MQ=255)
                                                                                                                            gggggACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGc                        <  1:1760855/149‑1 (MQ=255)
                                                                                                                            cggGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGc                        <  2:165384/150‑1 (MQ=255)
                                                                                                                                  cGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTg                  >  2:21159/1‑150 (MQ=255)
                                                                                                                                   gTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGc                 <  2:810201/150‑1 (MQ=255)
                                                                                                                                   atttGCCAGGCATTTGACTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGATTCGAGCGATTCCATTTCTTCTGCTTCGACATCATCGATGTGATCGTAACCTAACAAATCCAGACTGc                 >  2:180667/4‑150 (MQ=255)
                                                                                                                                    tCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCATCTGCATCGTCATCTTCGAAGTGATCGTAACCTAACAACTGCAGACTGcc                >  2:925951/1‑150 (MQ=255)
                                                                                                                                      tGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGt              >  1:1343680/1‑150 (MQ=255)
                                                                                                                                      tGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGACCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGt              >  1:1223090/1‑150 (MQ=255)
                                                                                                                                      tGCCAGTCAAATGCCTGGCAAATTATTCGTTCTCGGCAATGTACGGATCCTCATATCCAAGAGCAAGCATAATCTCTGTTTCGAGGGATTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACCAATGAAGACGGCCgg              >  1:1057181/1‑149 (MQ=255)
                                                                                                                                       gCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTg             <  2:1302654/150‑1 (MQ=255)
                                                                                                                                       gCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTg             <  1:1656128/150‑1 (MQ=255)
                                                                                                                                         cAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGca           >  1:1151231/1‑150 (MQ=255)
                                                                                                                                         cAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGca           >  2:1492939/1‑150 (MQ=255)
                                                                                                                                           gTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGcacc         >  1:493596/1‑150 (MQ=255)
                                                                                                                                               aaTGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCAccacca     <  2:1777857/150‑1 (MQ=255)
                                                                                                                                                  gCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCACCACCatat  <  2:506863/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCACCACCATAT  >  NC_000913/691718‑692013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: