| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 696,798 | A→G | 32.2% | noncoding (33/75 nt) | glnW ← | tRNA‑Gln |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 696,798 | 0 | A | G | 32.2% | 28.1 / 14.3 | 23 | noncoding (33/75 nt) | glnW | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base A (9/6); new base G (4/3); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01 | |||||||||||
GATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACA > NC_000913/696649‑696947 | caTACCTTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGCCCCAATTATTATTAGTTATGGGGTCAAACCATATGAGCTACGTATCCATATTGTTTCTTCGATGTCGGTGTTACGTGGATTCGAACCAGGGAATGCCGGTATCaa < 2:968449/149‑1 (MQ=21) ttGGTGGCTACGTCGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTa > 1:179816/1‑150 (MQ=255) cGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAt > 1:112333/1‑150 (MQ=255) tgtgACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATacacgg > 2:849574/1‑145 (MQ=255) cccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTg < 2:543704/150‑1 (MQ=255) tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAGCAACGGGCGGTGAccc > 2:553135/1‑150 (MQ=255) caGTGATGCTCTCTACCCCACTTAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACt < 1:1395139/149‑1 (MQ=25) aTGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaag < 2:112333/150‑1 (MQ=255) gtgCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAACGCTTACTCgaagaa > 2:593061/1‑150 (MQ=255) aCTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGt < 2:179816/150‑1 (MQ=255) gTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTc < 1:451347/150‑1 (MQ=255) cttgATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATC‑AAAACCGGTGcc < 2:1399537/147‑1 (MQ=255) cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCCATACCCGGGCGGTGACCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGGATCAACAACCGGTGc > 1:1633901/1‑150 (MQ=37) ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACAGGGCGGTGAACCGCTTAATCGAAGAAGATGGCTGGGGTACCTGGATTCGAACAAGGGAAATCCGGAATCAAAAACCGGTGCCATAc > 1:1388165/1‑150 (MQ=37) tcggAATGCCGGAATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATg < 1:1270634/84‑1 (MQ=255) tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATg > 2:1270634/3‑86 (MQ=18) gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCtt > 1:896742/1‑65 (MQ=17) gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCtt < 2:896742/65‑1 (MQ=17) ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCt > 2:1229860/1‑63 (MQ=17) ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCt < 1:1229860/63‑1 (MQ=17) cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCt > 2:1297581/1‑62 (MQ=17) cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCt < 1:1297581/62‑1 (MQ=17) ggTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAAAACCGGGCGGTGAACCGCTTACTCGAAGAAGATCGCTGGGGTACCTGGATTCGAACCAGGCAATGCCGGTATCAAAAACCGGGGCCTTACCGCTTGGCGATACCCCaa > 1:1612075/1‑149 (MQ=255) cAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCATTACCGCTTGGCGCTACCCCATCCGTa > 1:1608346/1‑150 (MQ=255) aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCAAAAAGACGGCGGTTACCCGCTAAGTCTAAGAAGCGCGCGGCGGTACCTCGAATCCAAACAGGGACAGCCGCGATCAAATTCAGCTTCAATACCGATTGGCGCTACCCCATcaatac > 2:1705671/1‑145 (MQ=255) aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCAAAAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACGCGGGAATGCCGGAATCAACAGCCGGTGCGTGACCGCTAGGCGATACCCCATCCGTAc > 2:1734769/1‑150 (MQ=37) aaaaCCGGTGCCTTACCGCTTGGCGATACCCCAATATCCGGGCGGTGAACCGCTTACTCGAAGAAGCTGGCTGGGGTACCTGGCTTCGAAACAGGGAATGCCGGTTTCAAAAACCGGTGCCTTACAGCTAGGCGATACCCCATCCGTACa > 1:1317443/1‑150 (MQ=38) | GATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACA > NC_000913/696649‑696947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |