Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 715,340 A→C 6.5% intergenic (+142/‑72) pgm → / → ybfP phosphoglucomutase/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913715,3400AC 6.5% 312.3 / 8.3 103intergenic (+142/‑72)pgm/ybfPphosphoglucomutase/lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (44/52);  new base C (3/4);  total (47/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCT  >  NC_000913/715192‑715489
                                                                                                                                                    |                                                                                                                                                     
cgcgTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGATACGCTTAACAGTCCTACGAGGATGGTTCAATATATTGAATTTAAGCGATTTTGTAGGCCTGATaat                                                                                                                                                      >  2:1629817/1‑149 (MQ=255)
 gcgTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAgg                                                                                                                                                     >  2:208884/1‑150 (MQ=255)
  cgTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCTCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGc                                                                                                                                                    <  2:710352/150‑1 (MQ=255)
     aaaCACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGtt                                                                                                                                                 >  2:1300456/1‑150 (MQ=255)
      aaCACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                <  1:1009045/150‑1 (MQ=255)
      aaCACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                <  1:390214/150‑1 (MQ=255)
      aaCACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                <  2:1043765/150‑1 (MQ=255)
      aaCACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                <  2:235527/150‑1 (MQ=255)
      aaCACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                >  1:1096971/1‑150 (MQ=255)
       acacATTTAATAAAAAAATGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCa                                                                                                                                               <  1:156708/150‑1 (MQ=255)
       acacATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCa                                                                                                                                               <  2:1085923/150‑1 (MQ=255)
            tttAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCAcgccg                                                                                                                                          <  1:1592999/150‑1 (MQ=255)
              taataaAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACCAGGATGGTGCTATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCa                                                                                                                                        >  2:1387828/1‑150 (MQ=255)
                ataaAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGAAAAACACAGAATTGCCTTATGCGCTACGCATATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAATGCGTTCCCGCCGCATc                                                                                                                                      >  2:368742/1‑150 (MQ=255)
                  aaaaaaaGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                                    >  2:1721136/1‑150 (MQ=255)
                   aaaaaaGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCGGATGGTGCAATATAGTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGAATCCgg                                                                                                                                   >  1:1711759/1‑150 (MQ=255)
                   aaaaaaGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                   <  2:236217/150‑1 (MQ=255)
                    aaagaGGGCGGTCGCAAGATCGCCCTTTTTTACGTATTACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTTCAATATATTGTATTTAAGCGATTTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                  <  2:1153949/150‑1 (MQ=25)
                     aaaaGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                                 <  1:723959/150‑1 (MQ=255)
                     aaaaGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                                 <  2:1386473/150‑1 (MQ=255)
                      aaaGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                                                >  1:799902/1‑150 (MQ=255)
                       aaGGGCGGTCGCAAGATCGCACTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTAAAGCGATTTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGccta                                                                                                                               <  1:1561386/150‑4 (MQ=21)
                        cgggCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaaaa                                                                                                                              <  2:1490740/149‑1 (MQ=255)
                        aGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaaaa                                                                                                                              <  1:1357798/150‑1 (MQ=255)
                           gCGGTCGCAAGATCGCCCTTTATTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGCGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAaaca                                                                                                                           <  1:920164/150‑1 (MQ=255)
                             ggTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAaacaac                                                                                                                         <  2:1405165/150‑1 (MQ=255)
                              gTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACg                                                                                                                        <  2:726376/150‑1 (MQ=255)
                              gTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACg                                                                                                                        >  1:438835/1‑150 (MQ=255)
                               tCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGACTACGAGGATGGTGCAATATATTGAATTTTAGCGATTTTGTCGGCCGGATAAGGCGTTCACGCCGCCTCCGGCAAAAACTACGa                                                                                                                       >  2:321165/1‑150 (MQ=25)
                                 gCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAac                                                                                                                     <  1:849539/150‑1 (MQ=255)
                                  cAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAaca                                                                                                                    >  2:1110462/1‑150 (MQ=255)
                                      aTCGCCCTTTTTTACGTATGACAAACACATAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGCATGGAGCAATAAATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACttt                                                                                                                <  1:45207/150‑1 (MQ=255)
                                         gCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTc                                                                                                             >  2:432300/1‑150 (MQ=255)
                                          cccTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTca                                                                                                            <  2:81550/150‑1 (MQ=255)
                                           ccTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTcaa                                                                                                           <  2:1363402/150‑1 (MQ=255)
                                             ttttttACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTcaaca                                                                                                         <  1:1104711/150‑1 (MQ=255)
                                               ttttACGTATGACAAACACAGAATTGCCTGATGCGCAACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACaaa                                                                                                       <  2:142376/150‑1 (MQ=255)
                                                 ttACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACt                                                                                                     <  2:1666088/150‑1 (MQ=255)
                                                  tACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTg                                                                                                    <  1:657165/150‑1 (MQ=255)
                                                     gTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGt                                                                                                 >  1:1822991/1‑150 (MQ=255)
                                                     gTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGt                                                                                                 <  2:1096971/150‑1 (MQ=255)
                                                        tGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGc                                                                                              <  1:123266/150‑1 (MQ=255)
                                                         gACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCt                                                                                             >  2:1179969/1‑150 (MQ=255)
                                                           cAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATAAATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCa                                                                                           >  1:1766864/1‑150 (MQ=255)
                                                                 caGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGaaa                                                                                     >  1:764716/1‑150 (MQ=255)
                                                                  aGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAAt                                                                                    >  1:852237/1‑150 (MQ=255)
                                                                    aaTTGCCTTATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATcc                                                                                  <  2:158389/150‑1 (MQ=255)
                                                                    aaTTGCCTGATGCGCTACGATTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACTAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATcc                                                                                  <  1:1141690/150‑1 (MQ=255)
                                                                     aTTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATccc                                                                                 >  1:464881/1‑150 (MQ=255)
                                                                       ggCCGGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCaa                                                                               <  2:1575640/149‑1 (MQ=255)
                                                                        gCCTGATGCGCTACGCTTATCAGGCCTACGATGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAAt                                                                              <  1:1300456/150‑1 (MQ=255)
                                                                            gATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAg                                                                          <  2:927595/150‑1 (MQ=255)
                                                                             aTGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGa                                                                         <  2:1057821/150‑1 (MQ=255)
                                                                             aTGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTACACGCCGCATCCGGCAAAACCAACGAACACTTTGTCAACATACTGCGTAGCTCATGGAAATCCCAATGAAGa                                                                         >  2:1610246/1‑150 (MQ=25)
                                                                               gcgcTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGAcc                                                                       <  1:47557/150‑1 (MQ=255)
                                                                                 gcTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCaa                                                                     >  1:618401/1‑150 (MQ=255)
                                                                                   tACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACa                                                                   >  2:472154/1‑150 (MQ=255)
                                                                                    aCGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAg                                                                  <  2:1212110/150‑1 (MQ=255)
                                                                                     cGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAgg                                                                 >  1:157273/1‑150 (MQ=255)
                                                                                          aTCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATACGGCGTTCACGCCGCATCCGGCAAAACCAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCt                                                            >  2:288921/1‑150 (MQ=255)
                                                                                            cAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAATGCGTTCACGCCGCATCCTGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTgg                                                          >  1:152616/1‑150 (MQ=255)
                                                                                             aGGCCTACGAGGATGGTGCAATAAAATGAATTTAAGAGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACCAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGgt                                                         >  1:1458008/1‑150 (MQ=255)
                                                                                               gCCTACGAGGATGGTGCAATATATTTAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGgtcg                                                       <  1:1219008/150‑1 (MQ=255)
                                                                                                 cTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGgtcgtc                                                     <  1:765267/150‑1 (MQ=255)
                                                                                                   aCGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCAt                                                   >  2:1034822/1‑150 (MQ=255)
                                                                                                    cGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATa                                                  >  2:1600817/1‑150 (MQ=255)
                                                                                                       ggATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAAAAAACTGAGTAGATCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAgtg                                               >  1:852126/1‑150 (MQ=255)
                                                                                                            gTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACt                                          >  1:1008300/1‑150 (MQ=255)
                                                                                                             tGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTa                                         <  2:799902/150‑1 (MQ=255)
                                                                                                                aaTATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAAtt                                      <  2:795628/150‑1 (MQ=255)
                                                                                                                 atatatTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTa                                     <  1:621826/150‑1 (MQ=255)
                                                                                                                  tatatTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTAc                                    >  2:1811152/1‑150 (MQ=255)
                                                                                                                    tatTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCg                                  >  1:468278/1‑150 (MQ=255)
                                                                                                                     atTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTAcctc                                 >  2:1214509/1‑148 (MQ=255)
                                                                                                                     atTGAATTCAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGc                                 >  1:538340/1‑150 (MQ=255)
                                                                                                                      ttGAATTTAATCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGcc                                <  2:376295/150‑1 (MQ=255)
                                                                                                                       tGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCcac                              <  1:396847/150‑1 (MQ=255)
                                                                                                                        gAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAACCTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCcac                              >  1:1145034/1‑150 (MQ=255)
                                                                                                                            ttAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTa                          >  2:1415872/1‑150 (MQ=255)
                                                                                                                            ttAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTa                          >  1:908948/1‑150 (MQ=255)
                                                                                                                              aaGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTAct                        >  2:1790629/1‑150 (MQ=255)
                                                                                                                                gCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTActgc                      <  1:1179969/150‑1 (MQ=255)
                                                                                                                                 cGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTActgct                     >  2:1173603/1‑150 (MQ=255)
                                                                                                                                  gATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTActgctg                    <  1:1383767/150‑1 (MQ=255)
                                                                                                                                     tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACt                 <  2:157273/150‑1 (MQ=255)
                                                                                                                                       tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCTAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGc               <  1:1146796/150‑1 (MQ=255)
                                                                                                                                       tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGc               <  1:226888/150‑1 (MQ=255)
                                                                                                                                       tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGc               >  2:755948/1‑150 (MQ=255)
                                                                                                                                         tttgCCGGATGCGGCGTGAACGCCTTATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCAt             >  1:1789414/4‑150 (MQ=255)
                                                                                                                                          aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATg            <  2:124564/150‑1 (MQ=255)
                                                                                                                                          aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATg            >  2:556429/1‑150 (MQ=255)
                                                                                                                                          aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATg            >  1:585376/1‑150 (MQ=255)
                                                                                                                                          aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAAAAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTTCTGACTGCATg            <  1:1629817/150‑1 (MQ=255)
                                                                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACGAATTACCGCCACATTACTGCGGACTGCATgc           >  2:189196/1‑150 (MQ=255)
                                                                                                                                            gCCGGATGCGGCGTGAACGCCTTATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATgcg          >  1:722336/1‑150 (MQ=255)
                                                                                                                                              cGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGcc        <  1:25788/150‑1 (MQ=255)
                                                                                                                                              cGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACAATACTGCTGACTGCATGCGcc        >  1:745733/1‑150 (MQ=255)
                                                                                                                                                   aaGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAAcc   <  1:1106811/150‑1 (MQ=255)
                                                                                                                                                    aGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCt  >  1:677236/1‑150 (MQ=255)
                                                                                                                                                    aGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCt  >  2:1028063/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCGTAAACACATTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCT  >  NC_000913/715192‑715489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: