Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 751,122 G→C 5.7% Q350E (CAG→GAG)  ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913751,1220GC 5.7% 331.2 / 7.6 102Q350E (CAG→GAG) ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (51/44);  new base C (2/4);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

Only 100 of 102 total aligned reads displayed.

CCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATA  >  NC_000913/750976‑751270
                                                                                                                                                  |                                                                                                                                                    
cccGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGAcc                                                                                                                                                   <  1:1756873/150‑1 (MQ=255)
cccGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTAAcc                                                                                                                                                   <  1:18369/150‑1 (MQ=255)
cccGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGAGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAATGTTTCATTTTCAGTTTTATGTGACAGGGAAGGCCGTGGCTGAcc                                                                                                                                                   >  2:257509/1‑150 (MQ=255)
 ccGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGAccc                                                                                                                                                  >  1:728383/1‑150 (MQ=255)
 ccGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTAATGTGACATGGAAGGCCGTGGCTGAccc                                                                                                                                                  <  2:587104/150‑1 (MQ=255)
      aCCCCTTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGGTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGc                                                                                                                                             <  2:1430167/150‑1 (MQ=255)
      aCCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGc                                                                                                                                             <  1:195120/150‑1 (MQ=255)
      aCCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGCGGGTCAGCCACGGc                                                                                                                                             <  2:1749836/150‑1 (MQ=255)
       cccATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGcc                                                                                                                                            <  1:1739788/150‑1 (MQ=255)
       cccATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCACCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGcc                                                                                                                                            <  1:789965/150‑1 (MQ=255)
            tGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGCGGGTCAGCCACGGccttcca                                                                                                                                       <  2:1822683/150‑6 (MQ=255)
            tGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGCGGGTCAGCCACGGccttcca                                                                                                                                       <  2:1815630/150‑6 (MQ=255)
            tGCGGCAGAATGGTAGTCATCATCAGAAAGCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGCGGGTCAGCCACGGccttcca                                                                                                                                       <  2:1043782/150‑6 (MQ=255)
              gggCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGTGGTGTTTGAGAGCATCCCCCAGCAAACTTCATTGCTAAAAACGGTTTCATTTTCAGTTTGATGTGACATGGATGGCCGTGGCTGACCCGCGGCCAATTtat                                                                                                                                     <  2:869504/149‑1 (MQ=255)
               ggCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGGACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTtata                                                                                                                                    <  1:1418423/150‑1 (MQ=255)
                 cAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGc                                                                                                                                  >  2:1337152/1‑150 (MQ=255)
                 cAGAAAGGAAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGGGTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGc                                                                                                                                  <  2:1736065/150‑1 (MQ=255)
                  aGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCg                                                                                                                                 <  2:1640695/150‑1 (MQ=255)
                       ggTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGAATCCCCCAGGAACCTTCATTGATAAAAAAGTTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCt                                                                                                                            >  1:924786/1‑150 (MQ=255)
                        gTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTg                                                                                                                           <  1:1494900/150‑1 (MQ=255)
                           gTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTcc                                                                                                                        >  1:1482157/1‑150 (MQ=255)
                            tcatcatcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTccc                                                                                                                       <  2:1518263/150‑1 (MQ=255)
                             catcatcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCt                                                                                                                      >  2:1400958/1‑150 (MQ=255)
                              atcatcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTg                                                                                                                     <  1:902197/150‑1 (MQ=255)
                                catcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGTAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCATGGc                                                                                                                   >  2:632673/1‑150 (MQ=255)
                                 atcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCg                                                                                                                  >  1:1551417/1‑150 (MQ=255)
                                  tcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGAATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGCCCGTGGCTGACCCGCGGCCAAATTATAGCGAACCTGTCCCTGGCGa                                                                                                                 >  1:238697/1‑150 (MQ=255)
                                        aTCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGATTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGACCCTGTCCCTGGCGAGTCAgg                                                                                                           >  2:894613/1‑150 (MQ=255)
                                        aTCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGGTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAgg                                                                                                           <  2:1357695/150‑1 (MQ=255)
                                        aTCAGCAGGCCGCCGTACAGAGAGGTGTTTGAGAGCATCCCCCAGGAAACATGATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGGGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAgg                                                                                                           <  1:1753144/150‑1 (MQ=255)
                                                 ccgccgTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTc                                                                                                  >  2:613559/1‑150 (MQ=255)
                                                 ccgccgTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTc                                                                                                  >  2:441873/1‑150 (MQ=255)
                                                 ccgccgTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTc                                                                                                  >  1:778401/1‑150 (MQ=255)
                                                    ccgTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAt                                                                                               <  2:1478728/150‑1 (MQ=255)
                                                     cgTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtg                                                                                              <  2:1033141/150‑1 (MQ=255)
                                                      ttACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtgc                                                                                             <  2:1560139/149‑1 (MQ=255)
                                                      gTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTCACCCGCGCCCAATATATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtgc                                                                                             >  1:563140/1‑150 (MQ=255)
                                                        aCAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtgctg                                                                                           <  1:832360/150‑1 (MQ=255)
                                                         cAGCGAGGTGTTTGAGAGCATCCCCCAGGAAAGTTCATTTCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtgctgc                                                                                          <  2:1276250/150‑1 (MQ=255)
                                                         cAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtgctgc                                                                                          <  2:1336241/150‑1 (MQ=255)
                                                          aGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAACGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGcc                                                                                         <  1:1088822/150‑1 (MQ=255)
                                                           gCGAGGTGTTTGAGAGCGTCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCg                                                                                        >  2:1707509/1‑150 (MQ=255)
                                                           gCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCg                                                                                        >  1:1474963/1‑150 (MQ=255)
                                                             gAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGaa                                                                                      >  2:123912/1‑150 (MQ=255)
                                                               ggtggtTGAGAGCATCCCCCAGGAAACTTCATAGCTAAAAACGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAAc                                                                                    <  1:468027/150‑1 (MQ=255)
                                                               ggTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAAc                                                                                    >  2:1734576/1‑150 (MQ=255)
                                                                  gtTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTg                                                                                 >  1:370332/1‑150 (MQ=255)
                                                                  gtTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTg                                                                                 >  2:1282443/1‑150 (MQ=255)
                                                                  gtTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAGGCTGCGGAACCCTg                                                                                 >  1:868124/1‑150 (MQ=255)
                                                                   tttGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTgg                                                                                >  1:1776039/1‑150 (MQ=255)
                                                                    ttGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGa                                                                               >  2:3963/1‑150 (MQ=255)
                                                                     tGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGaa                                                                              >  1:1199830/1‑150 (MQ=255)
                                                                     tGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGaa                                                                              >  2:1453986/1‑150 (MQ=255)
                                                                     tGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGaa                                                                              >  2:1328590/1‑150 (MQ=255)
                                                                     tGAGAGCAACCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATATTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGaa                                                                              <  1:1423077/150‑1 (MQ=255)
                                                                       agagCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATCCTGCCGAAACCTGGAAAt                                                                            >  1:281367/1‑150 (MQ=255)
                                                                        gagCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAAtt                                                                           >  2:1732819/1‑150 (MQ=255)
                                                                          ccATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGAAGTGACATGGAAGGCCGTGGCGGACCCGCTGACAATTTATAGCGATCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGt                                                                         <  1:116637/149‑1 (MQ=255)
                                                                              cccccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCAGAAACCTGTAAATTGTTCAc                                                                     >  2:495943/1‑150 (MQ=255)
                                                                                 ccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCg                                                                  >  1:1693405/1‑150 (MQ=255)
                                                                                  cAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGa                                                                 >  2:1425453/1‑150 (MQ=255)
                                                                                   aGGTAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGGTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGAc                                                                <  2:1682172/150‑1 (MQ=255)
                                                                                     gAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCa                                                              >  2:175122/1‑150 (MQ=255)
                                                                                         cTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGTAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCAtctt                                                          <  2:188132/150‑1 (MQ=255)
                                                                                          ttCATTGCTAAAAAAGGTTTCATTTTCCGTTTGATGTGACATGGAAGGCCGGGGCTGACCCTCGGCCAATTTATAGCGATCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCAgcttc                                                         <  1:26903/150‑1 (MQ=255)
                                                                                           tCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCAtcttct                                                        >  1:596026/1‑150 (MQ=255)
                                                                                             aTTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCttcttc                                                      <  2:449026/150‑1 (MQ=255)
                                                                                             aTTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCttcttc                                                      <  2:776083/150‑1 (MQ=255)
                                                                                               tGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGTTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCg                                                    <  2:387273/150‑1 (MQ=255)
                                                                                                gCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCTAAACCTGGAAATTGTTCACCCGACCATCTTCTTccct                                                   >  2:117021/1‑148 (MQ=255)
                                                                                                gCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGCCCATCTTCTTCCGt                                                   >  2:1730318/1‑150 (MQ=255)
                                                                                                  tAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCa                                                 >  1:1012910/1‑150 (MQ=255)
                                                                                                           tttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACAATCTTCTTCCGTCACTTTGACAt                                        >  1:1413791/1‑150 (MQ=255)
                                                                                                            ttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATc                                       <  1:1337152/150‑1 (MQ=255)
                                                                                                            ttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATc                                       >  2:1688178/1‑150 (MQ=255)
                                                                                                                ttttCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGc                                   <  2:1293761/150‑1 (MQ=255)
                                                                                                                        ttGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTg                           <  1:975788/150‑1 (MQ=255)
                                                                                                                         tGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGCCCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCGGa                          >  2:1294142/1‑150 (MQ=255)
                                                                                                                           aTGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAAc                        <  2:1544499/150‑1 (MQ=255)
                                                                                                                           aTGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAAc                        <  1:3963/150‑1 (MQ=255)
                                                                                                                             gtgACATGGAAGGCCGTGGCTGCCCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCCGCGTGCCGTGAACAg                      >  2:162150/1‑150 (MQ=255)
                                                                                                                             gtgACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCCTGCCCTGTAccg                      >  2:1144652/1‑148 (MQ=255)
                                                                                                                              tgACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCGGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGa                     >  1:1800459/1‑150 (MQ=255)
                                                                                                                              ggACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGa                     <  2:728383/149‑1 (MQ=255)
                                                                                                                               gACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGAc                    <  2:492400/150‑1 (MQ=255)
                                                                                                                                ccATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACt                   <  2:335894/149‑1 (MQ=255)
                                                                                                                                aCATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACt                   <  2:1259328/150‑1 (MQ=255)
                                                                                                                                aCATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCATCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACt                   <  2:1612742/150‑1 (MQ=255)
                                                                                                                                 cATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTg                  >  1:19078/1‑150 (MQ=255)
                                                                                                                                 cATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTg                  >  1:1635323/1‑150 (MQ=255)
                                                                                                                                  aTGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGa                 >  1:1072156/1‑150 (MQ=255)
                                                                                                                                   tGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGAt                >  1:1503424/1‑150 (MQ=255)
                                                                                                                                     gAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGCCATCCAGCGTGCCCTGAACAGCCTGATTg              >  2:1157905/1‑150 (MQ=255)
                                                                                                                                     gAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCTTGCCCTGAACAGACTGATTg              <  1:428947/150‑1 (MQ=255)
                                                                                                                                     gAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTg              >  1:397241/1‑150 (MQ=255)
                                                                                                                                     attggCCGCGGGTCAGCCACGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTg              >  2:1567486/4‑150 (MQ=255)
                                                                                                                                              ggCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGtcatca     <  2:1551417/150‑1 (MQ=255)
                                                                                                                                               gCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAACCGACCATCTTCTTCCGTCACTTTGACATCAAGCGTGCACTGCACAGACTGATTGAGGTCATCaa    >  2:269620/1‑150 (MQ=255)
                                                                                                                                                cTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGCCCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCtat   >  1:923845/1‑150 (MQ=255)
                                                                                                                                                 tGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCaata  >  1:427746/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATA  >  NC_000913/750976‑751270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: