Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 752,066 T→G 5.2% D35A (GAC→GCC)  ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913752,0660TG 5.2% 371.3 / 5.5 123D35A (GAC→GCC) ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (62/54);  new base G (2/5);  total (64/59)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGC  >  NC_000913/751917‑752214
                                                                                                                                                     |                                                                                                                                                    
cATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTGACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGt                                                                                                                                                      >  2:560744/1‑150 (MQ=255)
    gATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgcg                                                                                                                                                  <  2:194447/150‑1 (MQ=255)
    gATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgcg                                                                                                                                                  >  2:417215/1‑150 (MQ=255)
    gATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgcg                                                                                                                                                  >  1:1342020/1‑150 (MQ=255)
     aTGCATGTAAAGGTTTTGTCAGCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATATTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCccgc                                                                                                                                                 <  2:1433942/150‑1 (MQ=255)
        cATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAtt                                                                                                                                              >  1:694674/1‑150 (MQ=255)
        cATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAtt                                                                                                                                              >  1:821621/1‑150 (MQ=255)
        cATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAtt                                                                                                                                              >  2:87778/1‑150 (MQ=255)
         aTGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGTAATATCAATGCGGTCGCGCATTg                                                                                                                                             >  2:915807/1‑150 (MQ=255)
         aTGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTg                                                                                                                                             <  1:394029/150‑1 (MQ=255)
           ggAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAt                                                                                                                                           <  2:187146/150‑1 (MQ=255)
            gAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACAGTGAGCAATTACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAtt                                                                                                                                          >  1:138892/1‑150 (MQ=255)
              aTGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAtatt                                                                                                                                        <  1:1011065/150‑4 (MQ=255)
                ggTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATc                                                                                                                                      <  2:1750719/150‑1 (MQ=255)
                   tttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCgag                                                                                                                                   >  1:1401596/1‑150 (MQ=255)
                   tttGTCACCCTTTTTTTGACAGTTATTTTTTTGAGCTTTAGTGATTTTGTTGTTTTTCTCCTCAAAGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCgag                                                                                                                                   <  2:1185730/150‑1 (MQ=255)
                      gTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATCGATTTATCGAGAAc                                                                                                                                >  2:1331086/1‑150 (MQ=255)
                        cACCCTTTTTTTGCCAGTTAATTTTTTGCCCAATACTGATTTTGTTGTTATTCACCGCCACGCTAACAAAATATTCACCGGGAGCAATTACTCCTTTTTCCTTTAATAAAGAAATATCAATGCGGTCGCGCATTGATTTAACTAGAACAt                                                                                                                              >  2:98721/1‑150 (MQ=255)
                               tttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGAtt                                                                                                                       <  2:1372657/150‑1 (MQ=255)
                                ttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTg                                                                                                                      <  2:36548/150‑1 (MQ=255)
                                ttttGCCAGTTAATTTTTTGCACATTACTGATTTTGTTGTTATTCACCGCATCGCTTACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATTCGGTCGCGCATTGATTTATCGAGAACATTCAGATTg                                                                                                                      <  1:425543/150‑1 (MQ=255)
                                atttGACAGTTAATTATTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTAAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTg                                                                                                                      <  2:1308882/149‑1 (MQ=255)
                                       aGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGa                                                                                                               >  2:1302980/1‑150 (MQ=255)
                                       aGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGa                                                                                                               >  1:940063/1‑150 (MQ=255)
                                          tAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCg                                                                                                            <  1:636375/150‑1 (MQ=255)
                                          tAATTTTTTGCCCATTACTGATTTTGTTGTTATTAACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCg                                                                                                            <  2:955372/150‑1 (MQ=255)
                                              tttttGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCAt                                                                                                        <  2:540200/150‑1 (MQ=255)
                                                     ccATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACAGTCAGATTGAATTCGACCGCCATTGCGCaa                                                                                                 >  2:15532/1‑150 (MQ=255)
                                                      cATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAg                                                                                                <  2:1458886/150‑1 (MQ=255)
                                                      cATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAg                                                                                                >  1:8020/1‑150 (MQ=255)
                                                       aTTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAgg                                                                                               <  2:377563/150‑1 (MQ=255)
                                                         tACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCa                                                                                             >  1:1792060/1‑150 (MQ=255)
                                                               ttttGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCa                                                                                       >  1:349392/1‑150 (MQ=255)
                                                                 ttgttgTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATg                                                                                     <  1:417215/150‑1 (MQ=255)
                                                                 ttgttgGTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATg                                                                                     <  2:306277/150‑1 (MQ=255)
                                                                   gttgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGAc                                                                                   >  1:1677752/1‑150 (MQ=255)
                                                                    ttgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGAcc                                                                                  <  1:1732211/150‑1 (MQ=255)
                                                                         aTTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggca                                                                             >  1:463607/1‑150 (MQ=255)
                                                                         aTTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggca                                                                             <  2:162459/150‑1 (MQ=255)
                                                                         aTTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTAGACCGCCATTGCGCAAGGCATCGCCATGACcaggca                                                                             >  1:895165/1‑150 (MQ=255)
                                                                           tCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggcagg                                                                           >  2:674264/1‑150 (MQ=255)
                                                                            cACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGa                                                                          >  2:1680106/1‑150 (MQ=255)
                                                                              ccGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATa                                                                        >  1:450273/1‑150 (MQ=255)
                                                                               cGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGTTTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATAc                                                                       <  1:130196/150‑1 (MQ=255)
                                                                                    cGCTAACAAAATATTCACCGGGAGCAATGACTCCTGTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAGTTATCGCGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAag                                                                  <  2:340189/150‑1 (MQ=255)
                                                                                    cGCTAACAAAATATTCACCGGGAGAAATGACTCCTTTTTCCTTTAATAATGAAAAATCAATGCGGTCGCGCATTGATTTATCGAGAACATTAAGATTGAATTCGACCGCCATTGCGCAAGGCATCTCCATCAACAGGCAGGATACaaaaa                                                                  >  1:764798/1‑149 (MQ=255)
                                                                                     gCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAaga                                                                 <  2:891682/150‑1 (MQ=255)
                                                                                      cTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagag                                                                >  2:1506570/1‑150 (MQ=255)
                                                                                      cTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagag                                                                >  2:1201631/1‑150 (MQ=255)
                                                                                       tAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagaga                                                               <  1:319407/150‑1 (MQ=255)
                                                                                        aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagagag                                                              >  1:1251260/1‑150 (MQ=255)
                                                                                        aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagagag                                                              >  1:1070569/1‑150 (MQ=255)
                                                                                        aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagagag                                                              <  1:1060240/150‑1 (MQ=255)
                                                                                         aCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGt                                                             >  2:989954/1‑150 (MQ=255)
                                                                                          cAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGACATGACCAGGCAGGATACAAAAGAGAGTc                                                            >  1:1140340/1‑150 (MQ=255)
                                                                                           aaaaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCg                                                           <  1:1599377/150‑1 (MQ=255)
                                                                                           aaaaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCg                                                           >  2:486377/1‑150 (MQ=255)
                                                                                           aaaaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCg                                                           <  1:431017/150‑1 (MQ=255)
                                                                                             aaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGCCGAt                                                         >  1:1226509/1‑150 (MQ=255)
                                                                                             aaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATAGCAATGACCAGGCAGGATACAAAAGAGAGTCGAt                                                         >  1:604431/1‑150 (MQ=255)
                                                                                              atatTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGACATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGGCGATa                                                        <  1:280474/150‑1 (MQ=255)
                                                                                               tatTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATAACAATGCGGTCCCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGACATAGCGCAAGGCATCGCCATGCCCAGGCAGGATACAAAAGAGAGTCGATaa                                                       >  2:77766/1‑150 (MQ=255)
                                                                                                  tCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAata                                                    >  2:1328621/1‑150 (MQ=255)
                                                                                                  tCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCACAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAata                                                    <  2:1542645/150‑1 (MQ=255)
                                                                                                    aCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCATGCAGGATACAAAAGAGAGTCGATAAATAtt                                                  <  1:773233/150‑1 (MQ=255)
                                                                                                       gggAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCAc                                               <  2:481054/150‑1 (MQ=255)
                                                                                                         gAGCAATGACTCCTTTTTCCTTTAATAATTAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACgg                                             <  2:235958/150‑1 (MQ=255)
                                                                                                           gCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATTCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGgtg                                           <  1:503454/150‑1 (MQ=255)
                                                                                                             aaTGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTc                                         <  2:1073076/150‑1 (MQ=255)
                                                                                                              aTGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAATAGAGTCGATAAATATTAACGgtgtac                                        >  2:1752107/1‑148 (MQ=255)
                                                                                                                  cTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATAc                                    >  1:90769/1‑150 (MQ=255)
                                                                                                                          tCCTTTAATAATGAAATATCAATGCTGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGGCGATAAATATTCACGGTGTCCATACCTGATaaa                            <  1:1757259/150‑1 (MQ=255)
                                                                                                                            cTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                          >  2:1286606/1‑150 (MQ=255)
                                                                                                                            cTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                          >  1:9574/1‑150 (MQ=255)
                                                                                                                             tttAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAatat                         <  2:1615247/150‑1 (MQ=255)
                                                                                                                             tttAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATGCACGGTGTCCATACCTGATAAatat                         <  1:87778/150‑1 (MQ=255)
                                                                                                                              ttAATAATGAAATATCAATGCTGTCGCTCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGACATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATAtt                        <  2:1368115/150‑1 (MQ=255)
                                                                                                                              ttAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATAtt                        <  2:1032362/150‑1 (MQ=255)
                                                                                                                               taataaTGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATAttt                       >  1:1513941/1‑150 (MQ=255)
                                                                                                                                aataatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATAtttt                      <  2:1369341/150‑1 (MQ=255)
                                                                                                                                  taatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAt                    <  2:905084/150‑1 (MQ=255)
                                                                                                                                  taatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAt                    <  1:996604/150‑1 (MQ=255)
                                                                                                                                  taatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAt                    >  1:1779079/1‑150 (MQ=255)
                                                                                                                                   aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGCTACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAtc                   >  2:728455/1‑149 (MQ=255)
                                                                                                                                   aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATTAATATTTTATg                   >  2:1454737/1‑150 (MQ=255)
                                                                                                                                   aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATg                   <  2:986313/150‑1 (MQ=255)
                                                                                                                                    atGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGa                  >  2:1818637/1‑150 (MQ=255)
                                                                                                                                        aaTATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAgg              >  1:177000/1‑150 (MQ=255)
                                                                                                                                          tatCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTACATACCTGATAAATATTTTATTAAAggcg            >  2:429516/1‑150 (MQ=255)
                                                                                                                                          aatCAATGCGCGACCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAggcg            >  2:523788/2‑150 (MQ=255)
                                                                                                                                           atCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAggcgg           <  1:1422230/150‑1 (MQ=255)
                                                                                                                                            tCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCAGGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGGCCATACCTGATAAATATTTTATGAAAggcggc          >  2:1686506/1‑150 (MQ=255)
                                                                                                                                             aaaTGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGgcggcg         <  2:694674/149‑1 (MQ=255)
                                                                                                                                              aaTGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAggcggcta        >  1:999633/1‑148 (MQ=255)
                                                                                                                                               aTGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgat       <  2:1401596/150‑1 (MQ=255)
                                                                                                                                                tGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgatg      <  2:1070569/150‑1 (MQ=255)
                                                                                                                                                 gCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgatga     <  2:349392/150‑1 (MQ=255)
                                                                                                                                                  cGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgatgat    >  1:1042469/1‑150 (MQ=255)
                                                                                                                                                   ggTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGatgatg   >  2:606972/1‑150 (MQ=255)
                                                                                                                                                    gTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATgc  >  1:1024540/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGC  >  NC_000913/751917‑752214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: