Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 774,356 C→G 5.5% P16P (CCC→CCG ybgE → putative inner membrane protein in cydABX‑ybgE operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913774,3560CG 5.5% 384.0 / 7.8 121P16P (CCC→CCGybgEputative inner membrane protein in cydABX‑ybgE operon
Reads supporting (aligned to +/- strand):  ref base C (59/55);  new base G (4/3);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

CATGGATTCTGGGAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGTGTGATTCACGGCGT  >  NC_000913/774209‑774505
                                                                                                                                                   |                                                                                                                                                     
cATGGATTCTGGGAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAGCACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCt                                                                                                                                                     >  1:1095479/1‑150 (MQ=255)
     aTTCTGGGAACGCTTCTTGCCTGTTCGTTTGGGCTAATCACCGCGCTGGCGCTTGAACACGTCGACTCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATAATCGCGAATATGTATGCGGTATTGTACAAGCGCCCCCTGCggg                                                                                                                                                >  1:286640/1‑150 (MQ=255)
      ttCTGGGAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGATGGCGCTTGACCACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGAAGAGTAAGATTATCTCGAATTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGgc                                                                                                                                               >  1:1466570/1‑150 (MQ=255)
       tCTGGGAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGgcg                                                                                                                                              <  1:161644/150‑1 (MQ=255)
        cTGGGAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGgcgc                                                                                                                                             >  1:517819/1‑150 (MQ=255)
           ggAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCttt                                                                                                                                          >  2:285566/1‑150 (MQ=255)
           ggAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCttt                                                                                                                                          >  2:583302/1‑150 (MQ=255)
            gAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTc                                                                                                                                         >  1:410948/1‑150 (MQ=255)
             taCGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTcc                                                                                                                                        <  2:668172/149‑1 (MQ=255)
             aaCGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGCCATCTGATGAGTAAGAGTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCGCAGGGGGCGCttgtc                                                                                                                                        <  1:1205114/150‑4 (MQ=255)
              aCGCTTCTTGCCTGTTCGTTTGGGGTACTCCCCGGGCTGGCGCTTGAACTCGTCGAATCAGGCATAGCCGGTCATGAAGACATCTGATGTGTAAGATTATCGCGACTTTGTATGCGGTATTGGACAAGCGCCCCCTGCGGGCGCTTTCCt                                                                                                                                       <  2:388656/150‑1 (MQ=255)
              aCGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGCCAAGCGCCCCCTGCGGGCGCTTTCCt                                                                                                                                       >  1:1316338/1‑150 (MQ=255)
              aCGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCGCAGGGGGCGCttgtcc                                                                                                                                       <  2:859034/150‑5 (MQ=255)
                 cttcttGCCTGATCGTTTGGGGAAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGAGATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCg                                                                                                                                    <  2:449978/150‑1 (MQ=255)
                   tcttGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTg                                                                                                                                  <  2:522919/150‑1 (MQ=255)
                     ttGCCTGTTCGTTTGGGGTGATCACCGCGCTGGCGCTTGATCACGTCGAATCAGGCAAAGCCGGGCAAGAAGACATCTGATGAGTAAGTTTATAGCGACTTTGTATGCGGTAATGTACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGAt                                                                                                                                <  1:1083065/150‑1 (MQ=255)
                       gCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATgg                                                                                                                              <  2:1287322/150‑1 (MQ=255)
                            ttCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCtt                                                                                                                         <  1:1086693/150‑1 (MQ=255)
                              cGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCt                                                                                                                       >  2:1340299/1‑150 (MQ=255)
                               gTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTg                                                                                                                      >  1:1358946/1‑150 (MQ=255)
                                tttGGGGTAATCAACGCGCTGGCGCTTGAACACGTAGAATCAGGCAAAGCCGGTCAAGAAGACTTGTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGATTCTGt                                                                                                                     <  2:292666/150‑1 (MQ=255)
                                 ttGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGtt                                                                                                                    <  1:964861/150‑1 (MQ=255)
                                 ttGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGtt                                                                                                                    <  1:66346/150‑1 (MQ=255)
                                  tGGGGTAATAACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTa                                                                                                                   <  1:1217396/150‑1 (MQ=255)
                                   ggggTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAg                                                                                                                  >  1:1228755/1‑150 (MQ=255)
                                       tAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAgg                                                                                                              <  2:1271015/150‑1 (MQ=255)
                                        aaTCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGa                                                                                                             >  2:654628/1‑150 (MQ=255)
                                          tCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGatg                                                                                                           <  2:47998/150‑1 (MQ=255)
                                                  cTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGtttt                                                                                                   <  2:16614/150‑1 (MQ=255)
                                                   gggCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATTCGGTAATGGCCAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTg                                                                                                  <  1:1069666/149‑1 (MQ=255)
                                                       gcTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTAGGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGAc                                                                                              <  1:1183526/150‑1 (MQ=255)
                                                        cTTGAACACGTCGAATCAGTCAAAGCCTGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGAcc                                                                                             <  2:765942/150‑1 (MQ=255)
                                                          tGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCa                                                                                           <  2:1770542/150‑1 (MQ=255)
                                                          tGAACACGTCGAAGCAGGCAAAGCCGGGCAAGATTACATCTGAGGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCa                                                                                           <  2:518383/150‑1 (MQ=255)
                                                                cGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGt                                                                                     >  1:530125/1‑150 (MQ=255)
                                                                 gTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATTGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGtt                                                                                    >  1:746192/1‑150 (MQ=255)
                                                                    gAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTg                                                                                 >  2:220402/1‑150 (MQ=255)
                                                                    gAATCAGGCAAAGCCGGGCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTg                                                                                 <  2:331168/150‑1 (MQ=255)
                                                                     aaTCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGc                                                                                >  2:1281797/1‑150 (MQ=255)
                                                                     aaTCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGc                                                                                <  2:1466106/150‑1 (MQ=255)
                                                                      aTCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACACGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTGCTGTAAGCAGGATGTATGTTTTGGGACACATCTCGTTTTGcc                                                                               >  1:272067/1‑150 (MQ=255)
                                                                       tCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCcg                                                                              <  2:266237/150‑1 (MQ=255)
                                                                       gcAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAATATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCcg                                                                              <  2:1507834/149‑1 (MQ=255)
                                                                        cAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCGTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCagc                                                                             >  2:1293685/1‑150 (MQ=255)
                                                                            cAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAg                                                                         <  1:1680358/150‑1 (MQ=255)
                                                                              aaGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGAc                                                                       <  2:1587979/150‑1 (MQ=255)
                                                                               aGCCGGTCAAGAAGACATCTGATGAGTAAGATTATTGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGTGACCCATCGCGTTTTGCCGCGAATAcc                                                                      >  2:1561872/1‑150 (MQ=255)
                                                                               aGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGAcc                                                                      <  1:408526/150‑1 (MQ=255)
                                                                                    gTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGa                                                                 <  2:73979/150‑1 (MQ=255)
                                                                                      cAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAAc                                                               >  1:1438735/1‑150 (MQ=255)
                                                                                       aagaagACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACt                                                              <  1:1713418/150‑1 (MQ=255)
                                                                                        agatgaCATCTGATGAGTATGATTATCGCGAGTTTGTATGCGGTATTGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTg                                                             <  1:233072/150‑1 (MQ=255)
                                                                                        agaagaCATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTg                                                             >  1:706251/1‑150 (MQ=255)
                                                                                              cATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGCCCACTGAACTGGAAAta                                                       >  1:480190/1‑149 (MQ=255)
                                                                                               aTCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTTCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCt                                                      >  2:554189/1‑150 (MQ=255)
                                                                                               aTCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGATCTGGAAATCt                                                      >  1:1331209/1‑150 (MQ=255)
                                                                                               aTCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGATCTGGAAATCt                                                      >  1:1323849/1‑150 (MQ=255)
                                                                                                  tgatgaGTAAGATTCTCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGc                                                   <  2:894113/150‑1 (MQ=255)
                                                                                                  tgatgaGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGc                                                   <  2:641794/150‑1 (MQ=255)
                                                                                                   tatgaGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACACATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCa                                                  >  2:736314/2‑150 (MQ=255)
                                                                                                   gatgaGTATGATTATCGCGACTTTGTATGCGGTTATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCa                                                  <  1:86664/150‑1 (MQ=255)
                                                                                                      gaGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAATTGGAAATCTGGCATgg                                               >  2:921312/1‑150 (MQ=255)
                                                                                                      gaGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATgg                                               >  1:227600/1‑150 (MQ=255)
                                                                                                      gaGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATgg                                               >  1:1352663/1‑150 (MQ=255)
                                                                                                       aGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTCAACTGGAAATCTGGCATggg                                              >  1:180141/1‑149 (MQ=255)
                                                                                                        gTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGtt                                             <  2:530125/150‑1 (MQ=255)
                                                                                                        gTAAGATTATCGCGAATTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGGTGGATGTATGTTTTGGGTCCCATATAGTTTTGCCGCGAAGACCAGTTAACTGGAAATCTGGCATGGtt                                             >  1:1293840/1‑150 (MQ=255)
                                                                                                           aGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTATAGGGACCCATCTCGGTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTAt                                          >  2:1653561/1‑150 (MQ=255)
                                                                                                            gATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCGCTGCGTTCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTGGTTTTGCCTCGAAGACCAGTGATCTGGAAATCTGGGATGGTTTAtt                                         >  1:71204/1‑150 (MQ=255)
                                                                                                             aTTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCTAAGACAAGTGAACTGGAAATCTGGCATGGTTTATTg                                        >  1:1309166/1‑150 (MQ=255)
                                                                                                             aTTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTg                                        >  2:1585029/1‑150 (MQ=255)
                                                                                                              ttATCGCGACTTTGTATGCCGAAATCGACAAGCGCCCCCAGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGGTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGc                                       <  1:288287/150‑1 (MQ=255)
                                                                                                                 tcccgACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGATAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGa                                    <  2:592625/147‑1 (MQ=255)
                                                                                                                   gcgACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGAtg                                  >  1:1147135/1‑150 (MQ=255)
                                                                                                                   gcgACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTCTGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCGGAtg                                  >  2:292742/1‑150 (MQ=255)
                                                                                                                     gACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGAtgtg                                <  1:755017/150‑1 (MQ=255)
                                                                                                                     gACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGAtgtg                                <  1:583302/150‑1 (MQ=255)
                                                                                                                      ccTTTGTATGCGGTAATGGCCAAGCGCCCCCTCCGGGCGCTTTCCTTCGTGAGGGCGCTTCTGTTAGCAGGAGGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGTAAAACTGTCATGGTTTATTGCTGATGTgg                               <  2:214565/149‑1 (MQ=255)
                                                                                                                        tttGTATGCGGTAATGGACAAGCGCCCCCTGCGGGTGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGc                             >  1:1424617/1‑150 (MQ=255)
                                                                                                                        ttcgaaTGCGCTAATGCACAAGCCCCCCCTGCGGGCGCTTTCCTTCGGGATGGCGCTTCTGTTAGCAGGAGGAATGATTTGGGACCCAGCTCGTATTGCCGCGAAGACCAGGGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGc                             <  2:1358946/145‑1 (MQ=255)
                                                                                                                          tGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTTGAAATCTGGCAAGGTTTATTGCTGATGTGGGCCg                           >  2:709595/1‑150 (MQ=255)
                                                                                                                          tGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCg                           >  1:548856/1‑150 (MQ=255)
                                                                                                                            tATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTc                         >  2:1637355/1‑150 (MQ=255)
                                                                                                                             aTGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCt                        <  1:507272/150‑1 (MQ=255)
                                                                                                                             aTGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGGGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTGTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCt                        <  1:402565/150‑1 (MQ=255)
                                                                                                                               gCGGTAATGGACAAGCGCCCCCTGCGGGCGCGTTCCTTCGTGATGGCGCTTCGGTTAGCAGGATGTATGTTTTGGGACCCATGTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCGGATGTGGGCCGTCtgg                      >  2:1675355/1‑149 (MQ=255)
                                                                                                                                cGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCtgtg                     <  1:29355/150‑1 (MQ=255)
                                                                                                                                 ggTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGc                    >  2:791727/1‑150 (MQ=255)
                                                                                                                                 ggTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGCTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGc                    <  1:912311/150‑1 (MQ=255)
                                                                                                                                    aaTGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTgg                 <  2:1228755/150‑1 (MQ=255)
                                                                                                                                     gaaggaaaGCGCCCGCAGGGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGgt                >  2:358094/7‑150 (MQ=255)
                                                                                                                                      tGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGTATGAATGGTTTGGGACCCCTCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGCTGTGGGCCGTCTGTGCTGgtg               <  2:1095479/150‑1 (MQ=255)
                                                                                                                                       ggACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGgtgt              <  1:860442/150‑1 (MQ=255)
                                                                                                                                         gaaaGCGCCCGGAGGGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTATGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGTGCCGTCTGTGCTGGTGTGa            >  2:459617/3‑150 (MQ=255)
                                                                                                                                            aGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAAATGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGTGTGATTc         >  2:481356/1‑150 (MQ=255)
                                                                                                                                                cccccTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAATTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGTGTGATTCACgg     <  1:921312/150‑1 (MQ=255)
                                                                                                                                                cccccTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGTGTGATTCACgg     <  2:527473/150‑1 (MQ=255)
                                                                                                                                                cccccTGCGGGCGCTTTCCTTCGTGATCGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCCGGCATGGTTTATTGCTGATGGGGGCCGTCTGTGCTGGTGTGATTCACgg     <  1:117796/150‑1 (MQ=255)
                                                                                                                                                 ccccTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGTGTGATTCACGGc    <  1:421781/150‑1 (MQ=255)
                                                                                                                                                   ccTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCAATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGTGTGATTCACGGCGt  <  2:1767089/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CATGGATTCTGGGAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGTGTGATTCACGGCGT  >  NC_000913/774209‑774505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: