| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 136,532 | G→C | 5.4% | intergenic (‑68/+38) | speE ← / ← yacC | spermidine synthase (putrescine aminopropyltransferase)/PulS_OutS family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 136,532 | 0 | G | C | 5.4% | 283.7 / 7.3 | 92 | intergenic (‑68/+38) | speE/yacC | spermidine synthase (putrescine aminopropyltransferase)/PulS_OutS family protein |
| Reads supporting (aligned to +/- strand): ref base G (45/41); new base C (2/3); total (47/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01 | |||||||||||
GATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGG > NC_000913/136386‑136679 | gATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATg < 2:1055964/150‑1 (MQ=255) aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc < 2:1415291/150‑1 (MQ=255) aaCGTTATCTACCCCAACGTACTGCCCAAACTGGTCGGGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc < 2:306045/150‑1 (MQ=255) ttATCTAACGCAAAGTACTGCACAAACTGGTCTTGTAGCGTTTCATGCCACTTTTTTTTTTCTGCCTTGGGTTGATACCTCCTTTGTTAACACCCGTAAATAAAGGGCTCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAata < 2:1419837/150‑1 (MQ=255) tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGTGCATCCGCGGTCAatgt < 1:1238562/150‑3 (MQ=255) aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGGAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATAtt > 2:1473808/1‑150 (MQ=255) aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGTGCATCCGCGGTCAATGtt < 1:713261/150‑1 (MQ=255) aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGCCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATAtt > 1:236748/1‑150 (MQ=255) tACCGCAAAGTACTGCCCAAACTTGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGTGCATCCGCGGTCAATGttagc < 1:517992/150‑4 (MQ=255) tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTTTTAACACCCGTAAAAAAAGGGCGCAACATAATTGCTAACATTGACCGCGGATGCACGGTCAATATTTcg > 1:1343453/1‑149 (MQ=255) tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGGAATAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGGCAATATTTCt > 2:679570/1‑150 (MQ=255) tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTGTTTTATCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCt < 2:238105/150‑1 (MQ=255) aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTa < 1:615713/150‑1 (MQ=255) aCCGCAAACTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTATCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTa < 1:72626/150‑1 (MQ=255) ccGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTAGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTAc > 2:400810/1‑150 (MQ=255) aGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAg < 1:835200/150‑1 (MQ=255) gTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAgg < 2:778903/150‑1 (MQ=255) gTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAgg < 2:736281/150‑1 (MQ=255) tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTAta > 1:452467/1‑149 (MQ=255) gCCCAAACTGGTCGTGTAGCGGTTCATGCCACGGTTTTTTTTCGGCCATGGGGTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCGACATAATAGCTAACAATGACCGCGGATGCCCGGGCAATATTTCGTCAAAAAGGTATCa > 2:677447/1‑150 (MQ=255) cccAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATAAg > 2:1386668/1‑150 (MQ=255) cAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTAAAAAATCCTTTGTTAACAACCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGACAATATTTCTACAAAAAGGTATCAggg > 2:281558/1‑150 (MQ=255) ggTCGTGTAGCGTTTCATTCCACTGTTTTTTTTCTGCCATGGGTTGAAACCTCCTTTGTTAAAACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt > 2:72655/1‑150 (MQ=255) ggTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTTACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt > 1:1684094/1‑150 (MQ=255) ggTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt > 1:414366/1‑150 (MQ=255) ggTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGAAACCTCCATTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGATAACAATGACCCCGGATGCACGGTCAATATTTCAACAAAAAGGTATCCTGGattatt > 2:1338515/1‑150 (MQ=255) tgtAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACg > 1:1382973/1‑150 (MQ=255) tgtAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTATACAAAAAGGTATCAGGGATTATTTGACg < 1:208116/150‑1 (MQ=255) aGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAAAGGGCGCAACATAAAAGCTAACATTGACCGGGGATGCCCGGGCAAAATTTCATCAAAAAGGTATCATGGATTTTTTGACGTAgg > 2:1733251/1‑150 (MQ=255) cGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGc < 1:662322/150‑1 (MQ=255) gTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCa > 1:1681936/1‑150 (MQ=255) cgttCATGCCACTGTATTTTTTCGGCCATGGGTTGATACCTCCTTTGATAAGACCCGTAAAAAAATGGCGCAACATAATAGCAAACATTGACCGCGGAAGCACGGTCAATATTTCTACAAAAACGTATCAGGGATTATTTGACGTAGGCa < 1:421575/148‑1 (MQ=255) aTGCCACTGTTTTTTTTCGGCCATGGTTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAg < 1:1467721/150‑1 (MQ=255) tGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAgg < 1:1423284/150‑1 (MQ=255) ccACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCt > 2:962251/1‑150 (MQ=255) aCTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTa < 2:998146/150‑1 (MQ=255) cTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTaa < 1:950783/150‑1 (MQ=255) tGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCTGGGATTATTTGACGTAGGCAAGCAGGCTTAAg < 1:150543/150‑1 (MQ=255) tGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg > 1:1060374/1‑150 (MQ=255) tGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg < 1:1317428/150‑1 (MQ=255) tGTTTTTTTTCGGCCATGGGTTGATACCTCCTATGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCAAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg < 2:818504/150‑1 (MQ=255) tGTTATTTTTCTGCCATGGGTTTATACCTCCTTTGTTAACTCCCGTAAAAATAGGGCGCAAAATAATAGATAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg < 2:1187478/150‑1 (MQ=255) tgtttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAgg < 1:855775/148‑1 (MQ=255) ttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcg > 2:1165794/1‑150 (MQ=255) ttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATATCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcgcg > 1:191559/1‑150 (MQ=255) ggCCATGGGTTGATACCTCCTTTGTTAACACCCGAAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGAAGGATTAAGTAATCGCggtc > 1:1158671/1‑148 (MQ=255) cATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAg > 2:1459884/1‑150 (MQ=255) cATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAg > 2:1042396/1‑150 (MQ=255) cATGGGTTGATACCTCCTTTGTTAACACCAGTAAAAAAAGAGCTCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTCGGCACGCAGTCTTAAGGAATCGCGGGCCAg > 1:120131/1‑150 (MQ=255) tGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAggg > 2:1400939/1‑150 (MQ=255) ggTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCATCATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGTCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCt > 2:1274900/1‑150 (MQ=255) ggTCGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGGCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGTCAAGCAGGGAGAAGGAATCGCGGGCCAGGGCt > 2:865304/1‑150 (MQ=255) aTACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATATGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGct > 1:436584/1‑149 (MQ=255) cctcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAttt > 1:200277/1‑150 (MQ=255) ctcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtttt > 1:1812107/1‑150 (MQ=255) tcatttGTTAACACACGTAAAAAAAGGGCGCAACATAATCGCTAACATTGACGGCGGATGCACGGTCAATATTTCTACAAATAGGTATCAGGGATTATTTGACGTAGTCAAGCAGTCTTAAGTAATCGCGGGCCAGGGCTTTGCAttttt < 2:276186/147‑1 (MQ=255) cctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtttttt < 2:197578/150‑1 (MQ=255) ctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTTACATTGACCGCGGATGCACTGTCAATATTTCTACAAAAATGTATCAGGGATTATTTGACGTATGCAAGCAGGCTTAAGGAATCGCGGGCCAGGTCTTTGCATTTTTTa < 1:1301731/150‑1 (MQ=255) ctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTa > 2:1106294/1‑150 (MQ=255) tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTTTCCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGTGATTTTTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg < 1:3068/150‑1 (MQ=255) tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg < 1:370302/150‑1 (MQ=255) tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg < 1:281558/150‑1 (MQ=255) tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg < 1:1378422/150‑1 (MQ=255) tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg < 1:1515985/150‑1 (MQ=255) ttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGc < 1:400810/150‑1 (MQ=255) ttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGc < 2:1743061/150‑1 (MQ=255) gTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACGGCGGATGCACGGTCCATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAACCAGGCTTAAGGAATCGCGGGCCAGGGCTTTCCATTTTTTAGCgc > 2:238655/1‑149 (MQ=255) tAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACg > 1:1045730/1‑150 (MQ=255) aaCACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCCGGGCTTTGCATTTTTTAGCGACgg > 1:1385873/1‑150 (MQ=255) aaCACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACgg > 2:1415059/1‑150 (MQ=255) acCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTTACCGCGGATGCACGGTCAATATTTCTACAAAAATGAATCAGGGATTATTTGACGTAGGCAAGCAGGATTAAGGAATCGCGGGCCACGGCTTTGCATTTTTTAGCGACGGGaa > 2:128473/1‑150 (MQ=255) cccGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaat > 2:1013130/1‑150 (MQ=255) ccGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGGTTCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatg < 2:1588084/150‑1 (MQ=255) cGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatgc > 1:331403/1‑150 (MQ=255) gTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatgcc > 2:660007/1‑150 (MQ=255) gTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatgcc > 1:1086294/1‑150 (MQ=255) aGGGCTCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTCGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGc < 2:708390/150‑1 (MQ=255) ctcAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAACAGGAATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGACTCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTgag < 1:708902/148‑1 (MQ=255) gcAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTgaga > 2:284250/1‑150 (MQ=255) cAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTgagct > 1:204193/1‑148 (MQ=255) cAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGAt > 1:712193/1‑150 (MQ=255) aaataGCTAACATTGACCTCGTTTGCACGGTCAATATTTCTCCAAAAAGGTATCAGGGATTATATGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCgg < 1:952128/149‑1 (MQ=255) ataGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTa < 2:1278636/150‑1 (MQ=255) aGCTAACATTGAGCGCGGATGCACGGTCAATATTTCTACTAAAATGTATCAGGTATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGAGTCGGGAATGCCAATGCCGCTGAGATCGCGGTAGc < 1:11030/150‑1 (MQ=255) aGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGc < 2:1728606/150‑1 (MQ=255) aGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGCGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGc < 2:1418285/150‑1 (MQ=255) cTAACATTGACCGCTGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGTCCAGGGCTTTTCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTg < 1:1394810/150‑1 (MQ=255) aataTTGACCGTGCATCCGCGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTc > 1:761525/4‑150 (MQ=255) ataTTGACCGTGCATCCGCGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCt > 1:1305790/3‑150 (MQ=255) tGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTATCGACGGGAATGCCAATGCCGCTGAGATCTCGGTACCTGTCTTCAc > 2:1130589/1‑150 (MQ=255) ccGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGa > 2:43793/1‑150 (MQ=255) cgGAGGCACGCTCAATATTTCTACAAAACGGAAGCCGGGATTATTGGACGCAGCCAAGCCGCCTAACGGAAGCCCGGGCCAGGGCTTTGCATTTTTTAGCCCCGGCAATGCCAATCCCGCGGAGACCGCGGTAGCTGTCTTCACCGCggg < 2:592734/150‑1 (MQ=255) | GATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGG > NC_000913/136386‑136679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |