Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 876,560 C→G 5.5% A409G (GCA→GGA)  yliF → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913876,5600CG 5.5% 319.0 / 7.5 105A409G (GCA→GGA) yliFputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (41/57);  new base G (4/2);  total (46/59)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

Only 100 of 106 total aligned reads displayed.

GTAGATAAAGGTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAA  >  NC_000913/876412‑876705
                                                                                                                                                    |                                                                                                                                                 
gTAGATAAAGGTAAAGGCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGca                                                                                                                                                  <  2:1033834/150‑1 (MQ=255)
   gATAAAGGTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACAt                                                                                                                                               >  1:1375994/1‑150 (MQ=255)
   gATAAACGAAACGTCTCCCGCTTCGGCGGTGACGAATTTGCGGTTATTAATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACAt                                                                                                                                               <  1:331311/150‑1 (MQ=255)
      aaaGGTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCa                                                                                                                                            >  1:1186449/1‑150 (MQ=255)
         ggTAAAGTCTACCGCTTCGGCGGTGATGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaat                                                                                                                                         >  1:1463637/1‑150 (MQ=255)
         ggTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTGCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaat                                                                                                                                         <  2:104662/150‑1 (MQ=255)
          gTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAATAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatg                                                                                                                                        <  2:1084291/150‑1 (MQ=255)
          gTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatg                                                                                                                                        >  1:1089180/1‑150 (MQ=255)
            aaaGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatgaa                                                                                                                                      >  2:1254654/1‑150 (MQ=255)
                tCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTc                                                                                                                                  <  2:675570/150‑1 (MQ=255)
                 cTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTcc                                                                                                                                 <  2:1451573/150‑1 (MQ=255)
                 cTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTcc                                                                                                                                 <  1:1193925/150‑1 (MQ=255)
                 cTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTcc                                                                                                                                 <  2:939768/150‑1 (MQ=255)
                  tACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCATTTGAATGTGCt                                                                                                                                <  1:1077860/150‑1 (MQ=255)
                    ccGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTAc                                                                                                                              <  2:202352/150‑1 (MQ=255)
                    ccGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCATAc                                                                                                                              >  2:111713/1‑150 (MQ=255)
                      gCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCg                                                                                                                            >  1:1478526/1‑150 (MQ=255)
                        ttCGGCGGTGACGAATTTGCTGTGATTTATACTGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGTAATCGTTCATTTCCAGGTTGGAAGCATTAATTTAAGTACCAGTATCGGTGTATCACAATCAAATGAATGTGCTACCGTc                                                                                                                          <  2:1459906/150‑1 (MQ=255)
                         tCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCg                                                                                                                         <  2:1247578/150‑1 (MQ=255)
                          cggcggTGACGAATTTGCGGTGAGTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGGGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGGACATTCATTTGAATGTGCTAcaccgat                                                                                                                        <  1:1094524/150‑7 (MQ=255)
                           ggcggTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGaa                                                                                                                       >  1:418406/1‑150 (MQ=255)
                                  aCGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGa                                                                                                                <  1:1001257/150‑1 (MQ=255)
                                   cGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaa                                                                                                               <  1:1032982/150‑1 (MQ=255)
                                   cGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaa                                                                                                               <  2:1616011/150‑1 (MQ=255)
                                    gAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaaa                                                                                                              >  2:670142/1‑150 (MQ=255)
                                     taTTTGCGTTGATTTATACTGGTGTAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaaaa                                                                                                             <  1:175876/149‑1 (MQ=255)
                                           cGGTGATTTATACGGGTGGAAAGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTACCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTgg                                                                                                       >  2:818879/1‑150 (MQ=255)
                                            ggTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGc                                                                                                      <  2:1008527/150‑1 (MQ=255)
                                              tGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCgg                                                                                                    <  2:68373/150‑1 (MQ=255)
                                                     taCGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTATCACATTCAAATGAATGTCCTACAGTCGAACGCTTGAAAATGCTGGCGGATGAGCg                                                                                             >  1:662370/1‑150 (MQ=255)
                                                     taCGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCg                                                                                             >  2:702608/1‑150 (MQ=255)
                                                       cGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTTGGAAGCATTAATTTAAGAACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGc                                                                                           <  1:856153/150‑1 (MQ=255)
                                                          gTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGt                                                                                        <  1:384734/150‑1 (MQ=255)
                                                           tGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                       <  2:1716830/150‑1 (MQ=255)
                                                           tGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                       >  2:1340073/1‑150 (MQ=255)
                                                           gggAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                       <  2:1310878/149‑1 (MQ=255)
                                                            ggAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtat                                                                                      >  1:452404/1‑150 (MQ=255)
                                                             gAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTTAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtata                                                                                     <  2:231997/150‑1 (MQ=255)
                                                             gAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtata                                                                                     >  2:1763242/1‑150 (MQ=255)
                                                               aCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAag                                                                                   <  2:547060/150‑1 (MQ=255)
                                                               aCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAag                                                                                   <  1:139000/150‑1 (MQ=255)
                                                                cGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGTCTGTATAaga                                                                                  >  2:1277524/1‑150 (MQ=255)
                                                                 gCTGGAAGTGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAagag                                                                                 <  1:195545/150‑1 (MQ=255)
                                                                   tGGAAGAGTTGCTTTCGATTCTAATAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTATGTACCAGTATCGTTGTTGAACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATTAGCGTATGTATAAGAGTa                                                                               >  1:651470/1‑150 (MQ=255)
                                                                   tGGAAGAGTTGCTATCTATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTAAAAATGCTGGCGTATGAGCGGCTGTATAAGAGTa                                                                               <  1:70496/150‑1 (MQ=255)
                                                                   tGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTa                                                                               <  1:953899/150‑1 (MQ=255)
                                                                   gggAAGAGTTGGTATCGATTCTAAAAGAAATCGTTCATTTCCAGGGTGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTa                                                                               <  1:1299335/149‑1 (MQ=255)
                                                                    ggAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCTGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTaa                                                                              >  2:445062/1‑150 (MQ=255)
                                                                       agagTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGaa                                                                           >  1:912022/1‑150 (MQ=255)
                                                                       agagTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGaa                                                                           <  1:28366/150‑1 (MQ=255)
                                                                       agagTGGCTATCGATTATAAAAGAAATCGTTGATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCTGTGTAGGACATTCTTATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGATCGGCTGTATAAGAGtttgat                                                                           <  2:391873/150‑6 (MQ=255)
                                                                           ttGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAAc                                                                       <  1:428058/150‑1 (MQ=255)
                                                                            tGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACg                                                                      <  2:734380/150‑1 (MQ=255)
                                                                               tATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCa                                                                   >  1:746979/1‑150 (MQ=255)
                                                                               tATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTAAAAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGACGAACGCTTGAAAATGCTGAAGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCa                                                                   >  1:688883/1‑150 (MQ=255)
                                                                                aTCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTTCCAGTATCGGTGTAGCACATTCAAATGAATATCCTAACGTCGAACGCTTGAAAATTCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAg                                                                  >  1:27842/1‑150 (MQ=255)
                                                                                aTCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAg                                                                  >  1:1176702/1‑150 (MQ=255)
                                                                                 tCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCCTTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAgg                                                                 <  1:1091225/150‑1 (MQ=255)
                                                                                        tAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGa                                                          >  2:9082/1‑150 (MQ=255)
                                                                                        tAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGa                                                          >  1:1734087/1‑150 (MQ=255)
                                                                                         aaaaGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGAt                                                         >  1:74031/1‑150 (MQ=255)
                                                                                          aaaGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGAtt                                                        >  2:385973/1‑150 (MQ=255)
                                                                                           aaGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAAAGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTa                                                       >  1:120637/1‑150 (MQ=255)
                                                                                               aaTCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTg                                                   <  2:391005/150‑1 (MQ=255)
                                                                                                ctCGTTCATTTCCTGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTgg                                                  <  1:1716161/149‑1 (MQ=255)
                                                                                                aTCGTTCTTTTCCTGGTGGGAAGCATTAATTTAAGTATCAGTATGTGTGTAGCACATTCAAATGAATGTTCTACCGTCGAAGGCTTGAAAATGCTGGCGGATTAGGGGCTGTATAAGAGTAAGAAAAACCGCAGGGCACAGATTAGCTgg                                                  <  2:672646/150‑1 (MQ=255)
                                                                                                 tCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGc                                                 <  1:1093687/150‑1 (MQ=255)
                                                                                                   gTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAg                                               >  1:1306552/1‑150 (MQ=255)
                                                                                                   gTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTCGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAg                                               <  1:533818/150‑1 (MQ=255)
                                                                                                       aTTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCCAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAAt                                           <  2:1064664/150‑1 (MQ=255)
                                                                                                       aTTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATCAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTTTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAAt                                           <  2:889798/150‑1 (MQ=255)
                                                                                                            cAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCatta                                      >  2:1423187/1‑150 (MQ=255)
                                                                                                            cAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCatta                                      <  1:86239/150‑1 (MQ=255)
                                                                                                              ggTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCattatt                                    <  2:850322/150‑1 (MQ=255)
                                                                                                              ggTGGGAAGAATTAATTTTAGTACCAGTATGGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGATGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCattatt                                    <  2:367310/150‑1 (MQ=255)
                                                                                                               gttggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCTAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTc                                   <  1:898825/147‑1 (MQ=255)
                                                                                                               gTGGGAAGCTTTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGATTGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTc                                   <  2:396553/150‑1 (MQ=255)
                                                                                                                 gggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGc                                 >  1:710126/1‑150 (MQ=255)
                                                                                                                 gggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGc                                 <  2:1186449/150‑1 (MQ=255)
                                                                                                                   gAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAg                               >  2:122089/1‑150 (MQ=255)
                                                                                                                    aaGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCTTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAgg                              <  1:887059/150‑1 (MQ=255)
                                                                                                                      gCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGcc                            >  2:1243050/1‑150 (MQ=255)
                                                                                                                      gCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGcc                            >  2:1648017/1‑150 (MQ=255)
                                                                                                                       cATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCg                           <  2:1089180/150‑1 (MQ=255)
                                                                                                                        aTTAATTTAAGTACCAGTATCGGTGTGGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCgg                          >  1:734763/1‑150 (MQ=255)
                                                                                                                             tttAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACaaa                     >  1:1389906/1‑150 (MQ=255)
                                                                                                                               tAAGTACCACAATCGGTGTAGCACATTCAAATGAATGCCCTACCGTCGAACGCATGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCAGGCAGTAATCATTATTCGCAGGCCGGACAAATg                   <  1:698059/150‑1 (MQ=255)
                                                                                                                                 aGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAt                 >  2:1477187/1‑150 (MQ=255)
                                                                                                                                  gTACCAGTATCGGTGTAGCACATTCAAATTAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAtt                <  1:1596849/150‑1 (MQ=255)
                                                                                                                                       actATCGGTGTAGCACATTCAAATGAAAGTCCTACCCTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGAAAAAGATTAAGAAAAACGGCAGGGCACAGATTAGCAGGCAGAAATCATTATTCGCAGGCCGGCCAAATGATTTTGcc           >  2:1716437/3‑150 (MQ=255)
                                                                                                                                        gTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGccc          <  1:74156/150‑1 (MQ=255)
                                                                                                                                         taacGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCg         <  2:1785832/147‑1 (MQ=255)
                                                                                                                                           tCGGAGTAGCACATTCAAATGAATGTCCTACCGTCGAACCCGTGAAAATGCTGGCGGATGCGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATAATTCGCAGGCCGGACAAATGATTTTGCCCGGc       <  2:135346/150‑1 (MQ=255)
                                                                                                                                            gacggtAGGACATTCATTTGAATGTGCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGcc      >  2:1403028/5‑150 (MQ=255)
                                                                                                                                            cGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGcc      <  1:996520/150‑1 (MQ=255)
                                                                                                                                             acggtAGGACATTCATTTGAATGTGCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCt     >  1:1469521/4‑150 (MQ=255)
                                                                                                                                               tgtAGCACATTCAAATGAATGTCCTACCGTCGATCGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGa   >  1:1267393/1‑150 (MQ=255)
                                                                                                                                               ggtAGGACATTCATTTGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGa   >  1:173299/2‑150 (MQ=255)
                                                                                                                                                gtAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaa  <  1:1465069/150‑1 (MQ=255)
                                                                                                                                                gtAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaa  <  1:1472426/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTAGATAAAGGTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAA  >  NC_000913/876412‑876705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: