Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 876,569 A→T 6.7% N412I (AAT→ATT) ‡ yliF → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913876,5690AT 6.7% 292.3 / 7.7 102N412I (AAT→ATT) ‡yliFputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (39/56);  new base T (5/2);  total (44/58)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

Only 100 of 110 total aligned reads displayed.

GGTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACC  >  NC_000913/876421‑876716
                                                                                                                                                    |                                                                                                                                                   
ggTAAAGTCTACCGCTTCGGCGGTGATGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaat                                                                                                                                                    >  1:1463637/1‑150 (MQ=255)
ggTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaat                                                                                                                                                    >  2:388364/1‑150 (MQ=255)
ggTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTGCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaat                                                                                                                                                    <  2:104662/150‑1 (MQ=255)
 gTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAATAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatg                                                                                                                                                   <  2:1084291/150‑1 (MQ=255)
 gTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatg                                                                                                                                                   >  1:1089180/1‑150 (MQ=255)
   aaaGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatgaa                                                                                                                                                 >  2:1254654/1‑150 (MQ=255)
       tCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTc                                                                                                                                             <  2:675570/150‑1 (MQ=255)
        cTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTcc                                                                                                                                            <  2:939768/150‑1 (MQ=255)
        cTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTcc                                                                                                                                            <  1:1193925/150‑1 (MQ=255)
        cTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTcc                                                                                                                                            <  2:1451573/150‑1 (MQ=255)
         tACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCATTTGAATGTGCt                                                                                                                                           <  1:1077860/150‑1 (MQ=255)
           ccGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCATAc                                                                                                                                         >  2:111713/1‑150 (MQ=255)
             gCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCg                                                                                                                                       >  1:1478526/1‑150 (MQ=255)
               ttCGGCGGTGACGAATTTGCTGTGATTTATACTGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGTAATCGTTCATTTCCAGGTTGGAAGCATTAATTTAAGTACCAGTATCGGTGTATCACAATCAAATGAATGTGCTACCGTc                                                                                                                                     <  2:1459906/150‑1 (MQ=255)
                tCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCg                                                                                                                                    <  2:1247578/150‑1 (MQ=255)
                 cggcggTGACGAATTTGCGGTGAGTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGGGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGGACATTCATTTGAATGTGCTAcaccgat                                                                                                                                   <  1:1094524/150‑7 (MQ=255)
                  ggcggTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGaa                                                                                                                                  >  1:418406/1‑150 (MQ=255)
                         aCGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGa                                                                                                                           <  1:1001257/150‑1 (MQ=255)
                          cGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaa                                                                                                                          <  1:1032982/150‑1 (MQ=255)
                          cGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaa                                                                                                                          <  2:1616011/150‑1 (MQ=255)
                          cGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCAAATTCAATTGAATGTCCTACCGTCGAACGCATGaa                                                                                                                          >  1:902835/1‑150 (MQ=255)
                            taTTTGCGTTGATTTATACTGGTGTAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaaaa                                                                                                                        <  1:175876/149‑1 (MQ=255)
                                  cGGTGATTTATACGGGTGGAAAGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTACCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTgg                                                                                                                  >  2:818879/1‑150 (MQ=255)
                                   ggTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGc                                                                                                                 <  2:1008527/150‑1 (MQ=255)
                                     tGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCgg                                                                                                               <  2:68373/150‑1 (MQ=255)
                                            taCGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTATCACATTCAAATGAATGTCCTACAGTCGAACGCTTGAAAATGCTGGCGGATGAGCg                                                                                                        >  1:662370/1‑150 (MQ=255)
                                            taCGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCg                                                                                                        >  2:702608/1‑150 (MQ=255)
                                            taCGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCg                                                                                                        >  1:437749/1‑150 (MQ=255)
                                              cGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTTGGAAGCATTAATTTAAGAACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGc                                                                                                      <  1:856153/150‑1 (MQ=255)
                                                 gTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGt                                                                                                   <  1:384734/150‑1 (MQ=255)
                                                  tGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                                  <  2:1716830/150‑1 (MQ=255)
                                                  tGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                                  >  2:1340073/1‑150 (MQ=255)
                                                  gggAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                                  <  2:1310878/149‑1 (MQ=255)
                                                   ggAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtat                                                                                                 >  1:452404/1‑150 (MQ=255)
                                                    gAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTTAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtata                                                                                                <  2:231997/150‑1 (MQ=255)
                                                    gAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtata                                                                                                >  2:1763242/1‑150 (MQ=255)
                                                      aCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAag                                                                                              <  1:139000/150‑1 (MQ=255)
                                                      aCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAag                                                                                              <  2:547060/150‑1 (MQ=255)
                                                       cGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGTCTGTATAaga                                                                                             >  2:1277524/1‑150 (MQ=255)
                                                        gCTGGAAGTGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAagag                                                                                            <  1:195545/150‑1 (MQ=255)
                                                          tGGAAGAGTTGCTTTCGATTCTAATAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTATGTACCAGTATCGTTGTTGAACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATTAGCGTATGTATAAGAGTa                                                                                          >  1:651470/1‑150 (MQ=255)
                                                          tGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTa                                                                                          <  1:953899/150‑1 (MQ=255)
                                                          gggAAGAGTTGGTATCGATTCTAAAAGAAATCGTTCATTTCCAGGGTGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTa                                                                                          <  1:1299335/149‑1 (MQ=255)
                                                           ggAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCTGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTaa                                                                                         >  2:445062/1‑150 (MQ=255)
                                                              agagTGGCTATCGATTATAAAAGAAATCGTTGATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCTGTGTAGGACATTCTTATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGATCGGCTGTATAAGAGtttgat                                                                                      <  2:391873/150‑6 (MQ=255)
                                                                  ttGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAAc                                                                                  <  1:428058/150‑1 (MQ=255)
                                                                   tGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACg                                                                                 <  2:734380/150‑1 (MQ=255)
                                                                      tATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCa                                                                              >  1:746979/1‑150 (MQ=255)
                                                                      tATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTAAAAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGACGAACGCTTGAAAATGCTGAAGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCa                                                                              >  1:688883/1‑150 (MQ=255)
                                                                       aTCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTTCCAGTATCGGTGTAGCACATTCAAATGAATATCCTAACGTCGAACGCTTGAAAATTCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAg                                                                             >  1:27842/1‑150 (MQ=255)
                                                                       aTCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAg                                                                             >  1:1176702/1‑150 (MQ=255)
                                                                        tCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCCTTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAgg                                                                            <  1:1091225/150‑1 (MQ=255)
                                                                               tAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGa                                                                     >  2:9082/1‑150 (MQ=255)
                                                                               tAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGa                                                                     >  1:1734087/1‑150 (MQ=255)
                                                                                aaaaGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGAt                                                                    >  1:74031/1‑150 (MQ=255)
                                                                                 aaaGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGAtt                                                                   >  2:385973/1‑150 (MQ=255)
                                                                                      aaTCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTg                                                              <  2:391005/150‑1 (MQ=255)
                                                                                       ctCGTTCATTTCCTGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTgg                                                             <  1:1716161/149‑1 (MQ=255)
                                                                                       aTCGTTCTTTTCCTGGTGGGAAGCATTAATTTAAGTATCAGTATGTGTGTAGCACATTCAAATGAATGTTCTACCGTCGAAGGCTTGAAAATGCTGGCGGATTAGGGGCTGTATAAGAGTAAGAAAAACCGCAGGGCACAGATTAGCTgg                                                             <  2:672646/150‑1 (MQ=255)
                                                                                        tCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGc                                                            <  1:1093687/150‑1 (MQ=255)
                                                                                          gTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAg                                                          >  1:1306552/1‑150 (MQ=255)
                                                                                          gTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTCGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAg                                                          <  1:533818/150‑1 (MQ=255)
                                                                                              aTTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCCAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAAt                                                      <  2:1064664/150‑1 (MQ=255)
                                                                                              aTTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATCAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTTTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAAt                                                      <  2:889798/150‑1 (MQ=255)
                                                                                                   cAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCatta                                                 <  1:86239/150‑1 (MQ=255)
                                                                                                   cAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCatta                                                 >  2:1423187/1‑150 (MQ=255)
                                                                                                     ggTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCattatt                                               <  2:850322/150‑1 (MQ=255)
                                                                                                     ggTGGGAAGAATTAATTTTAGTACCAGTATGGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGATGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCattatt                                               <  2:367310/150‑1 (MQ=255)
                                                                                                      gttggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCTAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTc                                              <  1:898825/147‑1 (MQ=255)
                                                                                                      gTGGGAAGCTTTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGATTGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTc                                              <  2:396553/150‑1 (MQ=255)
                                                                                                       tGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCg                                             <  1:1422470/150‑1 (MQ=255)
                                                                                                        gggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGc                                            >  1:710126/1‑150 (MQ=255)
                                                                                                        gggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGc                                            <  2:1186449/150‑1 (MQ=255)
                                                                                                          gAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAg                                          >  2:122089/1‑150 (MQ=255)
                                                                                                           aaGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCTTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAgg                                         <  1:887059/150‑1 (MQ=255)
                                                                                                             gCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGcc                                       >  2:1648017/1‑150 (MQ=255)
                                                                                                             gCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGcc                                       >  2:1243050/1‑150 (MQ=255)
                                                                                                              cATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCg                                      <  2:1089180/150‑1 (MQ=255)
                                                                                                               aTTAATTTAAGTACCAGTATCGGTGTGGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCgg                                     >  1:734763/1‑150 (MQ=255)
                                                                                                                    tttAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACaaa                                >  1:1389906/1‑150 (MQ=255)
                                                                                                                      tAAGTACCACAATCGGTGTAGCACATTCAAATGAATGCCCTACCGTCGAACGCATGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCAGGCAGTAATCATTATTCGCAGGCCGGACAAATg                              <  1:698059/150‑1 (MQ=255)
                                                                                                                        aGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAt                            >  2:1477187/1‑150 (MQ=255)
                                                                                                                         gTACCAGTATCGGTGTAGCACATTCAAATTAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAtt                           <  1:1596849/150‑1 (MQ=255)
                                                                                                                              actATCGGTGTAGCACATTCAAATGAAAGTCCTACCCTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGAAAAAGATTAAGAAAAACGGCAGGGCACAGATTAGCAGGCAGAAATCATTATTCGCAGGCCGGCCAAATGATTTTGcc                      >  2:1716437/3‑150 (MQ=255)
                                                                                                                               gTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGccc                     <  1:74156/150‑1 (MQ=255)
                                                                                                                                taacGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCg                    <  2:1785832/147‑1 (MQ=255)
                                                                                                                                  tCGGAGTAGCACATTCAAATGAATGTCCTACCGTCGAACCCGTGAAAATGCTGGCGGATGCGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATAATTCGCAGGCCGGACAAATGATTTTGCCCGGc                  <  2:135346/150‑1 (MQ=255)
                                                                                                                                   gacggtAGGACATTCATTTGAATGTGCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGcc                 >  2:1329811/5‑150 (MQ=255)
                                                                                                                                   cGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGcc                 <  1:996520/150‑1 (MQ=255)
                                                                                                                                    acggtAGGACATTCATTTGAATGTGCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCt                >  1:1469521/4‑150 (MQ=255)
                                                                                                                                      tgtAGCACATTCAAATGAATGTCCTACCGTCGATCGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGa              >  1:1267393/1‑150 (MQ=255)
                                                                                                                                      ggtAGGACATTCATTTGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGa              >  1:173299/2‑150 (MQ=255)
                                                                                                                                       gtAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaa             <  1:1465069/150‑1 (MQ=255)
                                                                                                                                            acaTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGCCAAATGATGTTGCCCGGCCGGaagtaat        >  2:1173251/1‑150 (MQ=255)
                                                                                                                                             caTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaattaatt       <  2:330003/150‑1 (MQ=255)
                                                                                                                                             caTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaattaatt       <  2:1375994/150‑1 (MQ=255)
                                                                                                                                             caTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaattaatt       <  1:1763242/150‑1 (MQ=255)
                                                                                                                                               ttCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAAttaattaa     >  1:236677/1‑150 (MQ=255)
                                                                                                                                                tCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTaaa    >  1:1202223/1‑150 (MQ=255)
                                                                                                                                                  aaaTGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCACGGCCTGAATTAATTAAAcc  >  1:1268717/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACC  >  NC_000913/876421‑876716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: