Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 876,577 C→G 6.3% P415A (CCT→GCT)  yliF → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913876,5770CG 6.3% 314.2 / 7.8 100P415A (CCT→GCT) yliFputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (42/51);  new base G (3/3);  total (45/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTA  >  NC_000913/876428‑876722
                                                                                                                                                     |                                                                                                                                                 
tCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTc                                                                                                                                                   <  2:675570/150‑1 (MQ=255)
 cTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTcc                                                                                                                                                  <  1:1193925/150‑1 (MQ=255)
 cTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTcc                                                                                                                                                  <  2:1451573/150‑1 (MQ=255)
  tACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCATTTGAATGTGCt                                                                                                                                                 <  1:1077860/150‑1 (MQ=255)
    ccGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTAc                                                                                                                                               <  2:202352/150‑1 (MQ=255)
    ccGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCATAc                                                                                                                                               >  2:111713/1‑150 (MQ=255)
      gCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCg                                                                                                                                             >  1:1478526/1‑150 (MQ=255)
        ttCGGCGGTGACGAATTTGCTGTGATTTATACTGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGTAATCGTTCATTTCCAGGTTGGAAGCATTAATTTAAGTACCAGTATCGGTGTATCACAATCAAATGAATGTGCTACCGTc                                                                                                                                           <  2:1459906/150‑1 (MQ=255)
         tCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCg                                                                                                                                          <  2:1247578/150‑1 (MQ=255)
          cggcggTGACGAATTTGCGGTGAGTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGGGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGGACATTCATTTGAATGTGCTAcaccgat                                                                                                                                         <  1:1094524/150‑7 (MQ=255)
           ggcggTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGaa                                                                                                                                        >  1:418406/1‑150 (MQ=255)
                  aCGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGa                                                                                                                                 <  1:1001257/150‑1 (MQ=255)
                   cGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaa                                                                                                                                <  1:1032982/150‑1 (MQ=255)
                    gAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaaa                                                                                                                               >  2:670142/1‑150 (MQ=255)
                           cGGTGATTTATACGGGTGGAAAGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTACCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTgg                                                                                                                        >  2:818879/1‑150 (MQ=255)
                            ggTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGc                                                                                                                       <  2:1008527/150‑1 (MQ=255)
                              tGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCgg                                                                                                                     <  2:68373/150‑1 (MQ=255)
                                     taCGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTATCACATTCAAATGAATGTCCTACAGTCGAACGCTTGAAAATGCTGGCGGATGAGCg                                                                                                              >  1:662370/1‑150 (MQ=255)
                                     taCGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCg                                                                                                              >  2:702608/1‑150 (MQ=255)
                                     taCGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCg                                                                                                              >  1:437749/1‑150 (MQ=255)
                                       cGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTTGGAAGCATTAATTTAAGAACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGc                                                                                                            <  1:856153/150‑1 (MQ=255)
                                          gTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGt                                                                                                         <  1:384734/150‑1 (MQ=255)
                                           tGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                                        >  2:1340073/1‑150 (MQ=255)
                                           tGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                                        <  2:1716830/150‑1 (MQ=255)
                                           gggAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                                        <  2:1310878/149‑1 (MQ=255)
                                            ggAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtat                                                                                                       >  1:452404/1‑150 (MQ=255)
                                             gAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTTAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtata                                                                                                      <  2:231997/150‑1 (MQ=255)
                                             gAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtata                                                                                                      >  2:1763242/1‑150 (MQ=255)
                                               aCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAag                                                                                                    <  2:547060/150‑1 (MQ=255)
                                               aCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAag                                                                                                    <  1:139000/150‑1 (MQ=255)
                                                cGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGTCTGTATAaga                                                                                                   >  2:1277524/1‑150 (MQ=255)
                                                 gCTGGAAGTGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAagag                                                                                                  <  1:195545/150‑1 (MQ=255)
                                                   tGGAAGAGTTGCTTTCGATTCTAATAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTATGTACCAGTATCGTTGTTGAACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATTAGCGTATGTATAAGAGTa                                                                                                >  1:651470/1‑150 (MQ=255)
                                                   tGGAAGAGTTGCTATCTATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTAAAAATGCTGGCGTATGAGCGGCTGTATAAGAGTa                                                                                                <  1:70496/150‑1 (MQ=255)
                                                   tGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTa                                                                                                <  1:953899/150‑1 (MQ=255)
                                                   gggAAGAGTTGGTATCGATTCTAAAAGAAATCGTTCATTTCCAGGGTGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTa                                                                                                <  1:1299335/149‑1 (MQ=255)
                                                    ggAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCTGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTaa                                                                                               >  2:445062/1‑150 (MQ=255)
                                                       agagTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGaa                                                                                            >  1:912022/1‑150 (MQ=255)
                                                       agagTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGaa                                                                                            <  1:28366/150‑1 (MQ=255)
                                                       agagTGGCTATCGATTATAAAAGAAATCGTTGATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCTGTGTAGGACATTCTTATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGATCGGCTGTATAAGAGtttgat                                                                                            <  2:391873/150‑6 (MQ=255)
                                                           ttGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAAc                                                                                        <  1:428058/150‑1 (MQ=255)
                                                            tGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACg                                                                                       <  2:734380/150‑1 (MQ=255)
                                                               tATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCa                                                                                    >  1:746979/1‑150 (MQ=255)
                                                               tATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTAAAAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGACGAACGCTTGAAAATGCTGAAGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCa                                                                                    >  1:688883/1‑150 (MQ=255)
                                                                aTCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTTCCAGTATCGGTGTAGCACATTCAAATGAATATCCTAACGTCGAACGCTTGAAAATTCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAg                                                                                   >  1:27842/1‑150 (MQ=255)
                                                                aTCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAg                                                                                   >  1:1176702/1‑150 (MQ=255)
                                                                 tCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCCTTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAgg                                                                                  <  1:1091225/150‑1 (MQ=255)
                                                                        tAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGa                                                                           >  2:9082/1‑150 (MQ=255)
                                                                        tAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGa                                                                           >  1:1734087/1‑150 (MQ=255)
                                                                         aaaaGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGAt                                                                          >  1:74031/1‑150 (MQ=255)
                                                                          aaaGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGAtt                                                                         >  2:385973/1‑150 (MQ=255)
                                                                           aaGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAAAGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTa                                                                        >  1:120637/1‑150 (MQ=255)
                                                                               aaTCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTg                                                                    <  2:391005/150‑1 (MQ=255)
                                                                                ctCGTTCATTTCCTGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTgg                                                                   <  1:1716161/149‑1 (MQ=255)
                                                                                aTCGTTCTTTTCCTGGTGGGAAGCATTAATTTAAGTATCAGTATGTGTGTAGCACATTCAAATGAATGTTCTACCGTCGAAGGCTTGAAAATGCTGGCGGATTAGGGGCTGTATAAGAGTAAGAAAAACCGCAGGGCACAGATTAGCTgg                                                                   <  2:672646/150‑1 (MQ=255)
                                                                                 tCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGc                                                                  <  1:1093687/150‑1 (MQ=255)
                                                                                   gTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAg                                                                >  1:1306552/1‑150 (MQ=255)
                                                                                   gTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTCGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAg                                                                <  1:533818/150‑1 (MQ=255)
                                                                                       aTTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCCAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAAt                                                            <  2:1064664/150‑1 (MQ=255)
                                                                                       aTTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATCAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTTTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAAt                                                            <  2:889798/150‑1 (MQ=255)
                                                                                            cAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCatta                                                       >  2:1423187/1‑150 (MQ=255)
                                                                                            cAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCatta                                                       <  1:86239/150‑1 (MQ=255)
                                                                                              ggTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCattatt                                                     <  2:850322/150‑1 (MQ=255)
                                                                                              ggTGGGAAGAATTAATTTTAGTACCAGTATGGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGATGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCattatt                                                     <  2:367310/150‑1 (MQ=255)
                                                                                               gttggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCTAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTc                                                    <  1:898825/147‑1 (MQ=255)
                                                                                               gTGGGAAGCTTTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGATTGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTc                                                    <  2:396553/150‑1 (MQ=255)
                                                                                                tGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCg                                                   <  1:1422470/150‑1 (MQ=255)
                                                                                                 gggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGc                                                  <  2:1186449/150‑1 (MQ=255)
                                                                                                 gggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGc                                                  >  1:710126/1‑150 (MQ=255)
                                                                                                   gAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAg                                                >  2:122089/1‑150 (MQ=255)
                                                                                                    aaGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCTTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAgg                                               <  1:887059/150‑1 (MQ=255)
                                                                                                      gCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGcc                                             >  2:1243050/1‑150 (MQ=255)
                                                                                                      gCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGcc                                             >  2:1648017/1‑150 (MQ=255)
                                                                                                       cATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCg                                            <  2:1089180/150‑1 (MQ=255)
                                                                                                        aTTAATTTAAGTACCAGTATCGGTGTGGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCgg                                           >  1:734763/1‑150 (MQ=255)
                                                                                                             tttAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACaaa                                      >  1:1389906/1‑150 (MQ=255)
                                                                                                               tAAGTACCACAATCGGTGTAGCACATTCAAATGAATGCCCTACCGTCGAACGCATGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCAGGCAGTAATCATTATTCGCAGGCCGGACAAATg                                    <  1:698059/150‑1 (MQ=255)
                                                                                                                 aGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAt                                  >  2:1477187/1‑150 (MQ=255)
                                                                                                                  gTACCAGTATCGGTGTAGCACATTCAAATTAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAtt                                 <  1:1596849/150‑1 (MQ=255)
                                                                                                                       actATCGGTGTAGCACATTCAAATGAAAGTCCTACCCTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGAAAAAGATTAAGAAAAACGGCAGGGCACAGATTAGCAGGCAGAAATCATTATTCGCAGGCCGGCCAAATGATTTTGcc                            >  2:1716437/3‑150 (MQ=255)
                                                                                                                        gTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGccc                           <  1:74156/150‑1 (MQ=255)
                                                                                                                         taacGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCg                          <  2:1785832/147‑1 (MQ=255)
                                                                                                                           tCGGAGTAGCACATTCAAATGAATGTCCTACCGTCGAACCCGTGAAAATGCTGGCGGATGCGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATAATTCGCAGGCCGGACAAATGATTTTGCCCGGc                        <  2:135346/150‑1 (MQ=255)
                                                                                                                            gacggtAGGACATTCATTTGAATGTGCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGcc                       >  2:1403028/5‑150 (MQ=255)
                                                                                                                            gacggtAGGACATTCATTTGAATGTGCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGcc                       >  2:1329811/5‑150 (MQ=255)
                                                                                                                            cGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGcc                       <  1:996520/150‑1 (MQ=255)
                                                                                                                             acggtAGGACATTCATTTGAATGTGCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCt                      >  1:1469521/4‑150 (MQ=255)
                                                                                                                               tgtAGCACATTCAAATGAATGTCCTACCGTCGATCGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGa                    >  1:1267393/1‑150 (MQ=255)
                                                                                                                               ggtAGGACATTCATTTGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGa                    >  1:173299/2‑150 (MQ=255)
                                                                                                                                gtAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaa                   <  1:1465069/150‑1 (MQ=255)
                                                                                                                                gtAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaa                   <  1:1472426/150‑1 (MQ=255)
                                                                                                                                     acaTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGCCAAATGATGTTGCCCGGCCGGaagtaat              >  2:1173251/1‑150 (MQ=255)
                                                                                                                                      caTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaattaatt             <  2:1375994/150‑1 (MQ=255)
                                                                                                                                      caTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaattaatt             <  1:1763242/150‑1 (MQ=255)
                                                                                                                                      caTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaattaatt             <  2:330003/150‑1 (MQ=255)
                                                                                                                                        ttCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAAttaattaa           >  1:236677/1‑150 (MQ=255)
                                                                                                                                         tCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTaaa          >  1:1202223/1‑150 (MQ=255)
                                                                                                                                          cAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAAc         >  2:118705/1‑150 (MQ=255)
                                                                                                                                           aaaTGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCACGGCCTGAATTAATTAAAcc        >  1:1268717/1‑150 (MQ=255)
                                                                                                                                                 atgTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTa  <  2:1176702/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTA  >  NC_000913/876428‑876722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: