Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 136,535 G→C 5.4% intergenic (‑71/+35) speE ← / ← yacC spermidine synthase (putrescine aminopropyltransferase)/PulS_OutS family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913136,5350GC 5.4% 294.9 / 7.3 92intergenic (‑71/+35)speE/yacCspermidine synthase (putrescine aminopropyltransferase)/PulS_OutS family protein
Reads supporting (aligned to +/- strand):  ref base G (46/41);  new base C (2/3);  total (48/44)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

GATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTC  >  NC_000913/136386‑136684
                                                                                                                                                     |                                                                                                                                                     
gATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATg                                                                                                                                                       <  2:1055964/150‑1 (MQ=255)
       aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                                                                                                                                                <  2:1415291/150‑1 (MQ=255)
       aaCGTTATCTACCCCAACGTACTGCCCAAACTGGTCGGGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                                                                                                                                                <  2:306045/150‑1 (MQ=255)
           ttATCTAACGCAAAGTACTGCACAAACTGGTCTTGTAGCGTTTCATGCCACTTTTTTTTTTCTGCCTTGGGTTGATACCTCCTTTGTTAACACCCGTAAATAAAGGGCTCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAata                                                                                                                                            <  2:1419837/150‑1 (MQ=255)
            tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGTGCATCCGCGGTCAatgt                                                                                                                                           <  1:1238562/150‑3 (MQ=255)
             aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGGAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATAtt                                                                                                                                          >  2:1473808/1‑150 (MQ=255)
             aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGTGCATCCGCGGTCAATGtt                                                                                                                                          <  1:713261/150‑1 (MQ=255)
             aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGCCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATAtt                                                                                                                                          >  1:236748/1‑150 (MQ=255)
                tACCGCAAAGTACTGCCCAAACTTGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGTGCATCCGCGGTCAATGttagc                                                                                                                                       <  1:517992/150‑4 (MQ=255)
                tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTTTTAACACCCGTAAAAAAAGGGCGCAACATAATTGCTAACATTGACCGCGGATGCACGGTCAATATTTcg                                                                                                                                       >  1:1343453/1‑149 (MQ=255)
                tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGGAATAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGGCAATATTTCt                                                                                                                                       >  2:679570/1‑150 (MQ=255)
                tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTGTTTTATCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCt                                                                                                                                       <  2:238105/150‑1 (MQ=255)
                 aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTa                                                                                                                                      <  1:615713/150‑1 (MQ=255)
                 aCCGCAAACTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTATCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTa                                                                                                                                      <  1:72626/150‑1 (MQ=255)
                  ccGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTAGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTAc                                                                                                                                     >  2:400810/1‑150 (MQ=255)
                        aGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAg                                                                                                                               <  1:835200/150‑1 (MQ=255)
                         gTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAgg                                                                                                                              <  2:778903/150‑1 (MQ=255)
                         gTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAgg                                                                                                                              <  2:736281/150‑1 (MQ=255)
                             tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTAta                                                                                                                          >  1:452467/1‑149 (MQ=255)
                              gCCCAAACTGGTCGTGTAGCGGTTCATGCCACGGTTTTTTTTCGGCCATGGGGTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCGACATAATAGCTAACAATGACCGCGGATGCCCGGGCAATATTTCGTCAAAAAGGTATCa                                                                                                                         >  2:677447/1‑150 (MQ=255)
                               cccAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATAAg                                                                                                                        >  2:1386668/1‑150 (MQ=255)
                                 cAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTAAAAAATCCTTTGTTAACAACCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGACAATATTTCTACAAAAAGGTATCAggg                                                                                                                      >  2:281558/1‑150 (MQ=255)
                                       ggTCGTGTAGCGTTTCATTCCACTGTTTTTTTTCTGCCATGGGTTGAAACCTCCTTTGTTAAAACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt                                                                                                                >  2:72655/1‑150 (MQ=255)
                                       ggTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTTACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt                                                                                                                >  1:1684094/1‑150 (MQ=255)
                                       ggTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt                                                                                                                >  1:414366/1‑150 (MQ=255)
                                       ggTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGAAACCTCCATTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGATAACAATGACCCCGGATGCACGGTCAATATTTCAACAAAAAGGTATCCTGGattatt                                                                                                                >  2:1338515/1‑150 (MQ=255)
                                            tgtAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACg                                                                                                           >  1:1382973/1‑150 (MQ=255)
                                            tgtAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTATACAAAAAGGTATCAGGGATTATTTGACg                                                                                                           <  1:208116/150‑1 (MQ=255)
                                               aGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAAAGGGCGCAACATAAAAGCTAACATTGACCGGGGATGCCCGGGCAAAATTTCATCAAAAAGGTATCATGGATTTTTTGACGTAgg                                                                                                       >  2:1733251/1‑150 (MQ=255)
                                                 cGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGc                                                                                                      <  1:662322/150‑1 (MQ=255)
                                                  gTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCa                                                                                                     >  1:1681936/1‑150 (MQ=255)
                                                  cgttCATGCCACTGTATTTTTTCGGCCATGGGTTGATACCTCCTTTGATAAGACCCGTAAAAAAATGGCGCAACATAATAGCAAACATTGACCGCGGAAGCACGGTCAATATTTCTACAAAAACGTATCAGGGATTATTTGACGTAGGCa                                                                                                     <  1:421575/148‑1 (MQ=255)
                                                       aTGCCACTGTTTTTTTTCGGCCATGGTTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAg                                                                                                <  1:1467721/150‑1 (MQ=255)
                                                        tGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAgg                                                                                               <  1:1423284/150‑1 (MQ=255)
                                                          ccACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCt                                                                                             >  2:962251/1‑150 (MQ=255)
                                                            aCTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTa                                                                                           <  2:998146/150‑1 (MQ=255)
                                                             cTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTaa                                                                                          <  1:950783/150‑1 (MQ=255)
                                                              tGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCTGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         <  1:150543/150‑1 (MQ=255)
                                                              tGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         >  1:1060374/1‑150 (MQ=255)
                                                              tGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         <  1:1317428/150‑1 (MQ=255)
                                                              tGTTTTTTTTCGGCCATGGGTTGATACCTCCTATGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCAAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         <  2:818504/150‑1 (MQ=255)
                                                              tGTTATTTTTCTGCCATGGGTTTATACCTCCTTTGTTAACTCCCGTAAAAATAGGGCGCAAAATAATAGATAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         <  2:1187478/150‑1 (MQ=255)
                                                               tgtttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAgg                                                                                        <  1:855775/148‑1 (MQ=255)
                                                                    ttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcg                                                                                   >  2:1165794/1‑150 (MQ=255)
                                                                      ttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATATCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcgcg                                                                                 >  1:191559/1‑150 (MQ=255)
                                                                         ggCCATGGGTTGATACCTCCTTTGTTAACACCCGAAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGAAGGATTAAGTAATCGCggtc                                                                              >  1:1158671/1‑148 (MQ=255)
                                                                            cATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAg                                                                           >  2:1459884/1‑150 (MQ=255)
                                                                            cATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAg                                                                           >  2:1042396/1‑150 (MQ=255)
                                                                            cATGGGTTGATACCTCCTTTGTTAACACCAGTAAAAAAAGAGCTCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTCGGCACGCAGTCTTAAGGAATCGCGGGCCAg                                                                           >  1:120131/1‑150 (MQ=255)
                                                                              tGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAggg                                                                         >  2:1400939/1‑150 (MQ=255)
                                                                                ggTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCATCATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGTCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCt                                                                       >  2:1274900/1‑150 (MQ=255)
                                                                                ggTCGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGGCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGTCAAGCAGGGAGAAGGAATCGCGGGCCAGGGCt                                                                       >  2:865304/1‑150 (MQ=255)
                                                                                     aTACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATATGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGct                                                                  >  1:436584/1‑149 (MQ=255)
                                                                                        cctcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAttt                                                               >  1:200277/1‑150 (MQ=255)
                                                                                         ctcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtttt                                                              >  1:1812107/1‑150 (MQ=255)
                                                                                          tcatttGTTAACACACGTAAAAAAAGGGCGCAACATAATCGCTAACATTGACGGCGGATGCACGGTCAATATTTCTACAAATAGGTATCAGGGATTATTTGACGTAGTCAAGCAGTCTTAAGTAATCGCGGGCCAGGGCTTTGCAttttt                                                             <  2:276186/147‑1 (MQ=255)
                                                                                           cctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtttttt                                                            <  2:197578/150‑1 (MQ=255)
                                                                                            ctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTTACATTGACCGCGGATGCACTGTCAATATTTCTACAAAAATGTATCAGGGATTATTTGACGTATGCAAGCAGGCTTAAGGAATCGCGGGCCAGGTCTTTGCATTTTTTa                                                           <  1:1301731/150‑1 (MQ=255)
                                                                                            ctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTa                                                           >  2:1106294/1‑150 (MQ=255)
                                                                                             tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTTTCCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGTGATTTTTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:3068/150‑1 (MQ=255)
                                                                                             tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:370302/150‑1 (MQ=255)
                                                                                             tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:1378422/150‑1 (MQ=255)
                                                                                             tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:281558/150‑1 (MQ=255)
                                                                                             tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:1515985/150‑1 (MQ=255)
                                                                                              ttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGc                                                         <  1:400810/150‑1 (MQ=255)
                                                                                              ttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGc                                                         <  2:1743061/150‑1 (MQ=255)
                                                                                                gTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACGGCGGATGCACGGTCCATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAACCAGGCTTAAGGAATCGCGGGCCAGGGCTTTCCATTTTTTAGCgc                                                       >  2:238655/1‑149 (MQ=255)
                                                                                                  tAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACg                                                     >  1:1045730/1‑150 (MQ=255)
                                                                                                   aaCACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCCGGGCTTTGCATTTTTTAGCGACgg                                                    >  1:1385873/1‑150 (MQ=255)
                                                                                                   aaCACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACgg                                                    >  2:1415059/1‑150 (MQ=255)
                                                                                                      acCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTTACCGCGGATGCACGGTCAATATTTCTACAAAAATGAATCAGGGATTATTTGACGTAGGCAAGCAGGATTAAGGAATCGCGGGCCACGGCTTTGCATTTTTTAGCGACGGGaa                                                 >  2:128473/1‑150 (MQ=255)
                                                                                                       cccGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaat                                                >  2:1013130/1‑150 (MQ=255)
                                                                                                        ccGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGGTTCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatg                                               <  2:1588084/150‑1 (MQ=255)
                                                                                                         cGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatgc                                              >  1:331403/1‑150 (MQ=255)
                                                                                                          gTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatgcc                                             >  1:1086294/1‑150 (MQ=255)
                                                                                                          gTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatgcc                                             >  2:660007/1‑150 (MQ=255)
                                                                                                                  aGGGCTCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTCGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGc                                     <  2:708390/150‑1 (MQ=255)
                                                                                                                      ctcAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAACAGGAATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGACTCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTgag                                 <  1:708902/148‑1 (MQ=255)
                                                                                                                       gcAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTgaga                                >  2:284250/1‑150 (MQ=255)
                                                                                                                        cAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTgagct                               >  1:204193/1‑148 (MQ=255)
                                                                                                                        cAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGAt                               >  1:712193/1‑150 (MQ=255)
                                                                                                                             aaataGCTAACATTGACCTCGTTTGCACGGTCAATATTTCTCCAAAAAGGTATCAGGGATTATATGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCgg                          <  1:952128/149‑1 (MQ=255)
                                                                                                                               ataGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTa                        <  2:1278636/150‑1 (MQ=255)
                                                                                                                                 aGCTAACATTGAGCGCGGATGCACGGTCAATATTTCTACTAAAATGTATCAGGTATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGAGTCGGGAATGCCAATGCCGCTGAGATCGCGGTAGc                      <  1:11030/150‑1 (MQ=255)
                                                                                                                                 aGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGc                      <  2:1728606/150‑1 (MQ=255)
                                                                                                                                 aGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGCGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGc                      <  2:1418285/150‑1 (MQ=255)
                                                                                                                                   cTAACATTGACCGCTGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGTCCAGGGCTTTTCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTg                    <  1:1394810/150‑1 (MQ=255)
                                                                                                                                     aataTTGACCGTGCATCCGCGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTc                  >  1:761525/4‑150 (MQ=255)
                                                                                                                                      ataTTGACCGTGCATCCGCGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCt                 >  1:1305790/3‑150 (MQ=255)
                                                                                                                                          tGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTATCGACGGGAATGCCAATGCCGCTGAGATCTCGGTACCTGTCTTCAc             >  2:1130589/1‑150 (MQ=255)
                                                                                                                                             ccGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGa          >  2:43793/1‑150 (MQ=255)
                                                                                                                                               gcgtaggCACGGGAAATATTTCTATATATACGTATCAGGGATTATTTGACGTAGGCAAGCTGGCTTAAGGACTCGCGGGCGTGTGCTTTGCATTTTTTAGCGACTGGAATGACAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAgg        <  1:285432/144‑1 (MQ=255)
                                                                                                                                                cgGAGGCACGCTCAATATTTCTACAAAACGGAAGCCGGGATTATTGGACGCAGCCAAGCCGCCTAACGGAAGCCCGGGCCAGGGCTTTGCATTTTTTAGCCCCGGCAATGCCAATCCCGCGGAGACCGCGGTAGCTGTCTTCACCGCggg       <  2:592734/150‑1 (MQ=255)
                                                                                                                                                    tGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCttt   >  1:441827/1‑150 (MQ=255)
                                                                                                                                                     gCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTc  <  2:169439/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTC  >  NC_000913/136386‑136684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑