Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 894,952 T→A 7.1% intergenic (+56/‑39) potF → / → potG putrescine ABC transporter periplasmic binding protein/putrescine ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913894,9520TA 7.1% 303.2 / 9.7 107intergenic (+56/‑39)potF/potGputrescine ABC transporter periplasmic binding protein/putrescine ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (55/44);  new base A (2/6);  total (57/50)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 111 total aligned reads displayed.

TGTTCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGAT  >  NC_000913/894803‑895101
                                                                                                                                                     |                                                                                                                                                     
tGTTCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCAtt                                                                                                                                                       <  1:801574/150‑1 (MQ=255)
 gTTCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTa                                                                                                                                                      >  2:796362/1‑150 (MQ=255)
  ttCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTAt                                                                                                                                                     >  2:158132/1‑150 (MQ=255)
      tGCGAAGCTGTTCACTCTGAAAGTACAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTg                                                                                                                                                 >  1:1167457/1‑150 (MQ=255)
      ggCGAAGCTGTTCACTCTGAACGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTg                                                                                                                                                 <  2:1364875/149‑1 (MQ=255)
      ggCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTg                                                                                                                                                 <  1:1610354/149‑1 (MQ=255)
        cGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcg                                                                                                                                               <  2:773119/150‑1 (MQ=255)
          aaGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaa                                                                                                                                             <  1:616582/150‑3 (MQ=255)
          aaGCGGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaa                                                                                                                                             <  1:180094/150‑3 (MQ=255)
           aGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCtt                                                                                                                                            <  2:1342081/150‑1 (MQ=255)
           aGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaat                                                                                                                                            <  2:535139/150‑4 (MQ=255)
            gCTGTTCACTCTGAAAGTGCAGGATCCGAAATTCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTg                                                                                                                                           <  2:404200/150‑1 (MQ=255)
            gCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTg                                                                                                                                           <  2:890057/150‑1 (MQ=255)
             cTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaattg                                                                                                                                          <  2:1157561/150‑6 (MQ=255)
             cTGTGCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGc                                                                                                                                          <  1:1729165/150‑1 (MQ=255)
              tGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGca                                                                                                                                         >  1:624891/1‑150 (MQ=255)
              tGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGca                                                                                                                                         >  1:679675/1‑150 (MQ=255)
              tGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGca                                                                                                                                         <  2:781914/150‑1 (MQ=255)
              ggTTCCCTCTGAAATTGCCCGATCCGAAAATCCCCCGTGTGCGCACCCGCGCGTGGACCAAAGGGAACAGCCGAAAAAAAACCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaattgc                                                                                                                                         <  1:884325/149‑7 (MQ=255)
              ggTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAACTAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGca                                                                                                                                         <  1:84346/149‑1 (MQ=255)
                ttCACTCTGAAAGTCCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcaca                                                                                                                                       <  2:74823/150‑1 (MQ=255)
                  cACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcacaca                                                                                                                                     >  2:55565/1‑150 (MQ=255)
                    ctctGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGCGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCGCACAtt                                                                                                                                   >  2:326349/1‑150 (MQ=255)
                    ctctGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACAtt                                                                                                                                   <  2:1711274/150‑1 (MQ=255)
                     tctGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTc                                                                                                                                  <  1:1079724/150‑1 (MQ=255)
                     tctGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGGAGATGCCGGAGGGGCGCCCCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACTTTc                                                                                                                                  >  2:264657/1‑150 (MQ=255)
                      ctGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCa                                                                                                                                 >  2:1460851/1‑150 (MQ=255)
                      ctGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGCGCGCTTGCACACATTCa                                                                                                                                 >  2:1170125/1‑150 (MQ=255)
                         aaaGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATg                                                                                                                              <  1:218237/150‑1 (MQ=255)
                          aaGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAAAAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGc                                                                                                                             <  1:421443/150‑1 (MQ=255)
                            gTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCg                                                                                                                           >  1:1322388/1‑150 (MQ=255)
                             tGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCgg                                                                                                                          <  2:1141620/150‑1 (MQ=255)
                              gCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGga                                                                                                                         >  1:239731/1‑150 (MQ=255)
                               cAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGgag                                                                                                                        >  2:1200531/1‑150 (MQ=255)
                                aGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGgaga                                                                                                                       <  2:1505802/150‑1 (MQ=255)
                                 ggATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGagag                                                                                                                      >  2:1155889/1‑150 (MQ=255)
                                 ggATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGagag                                                                                                                      >  1:1270885/1‑150 (MQ=255)
                                       gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCg                                                                                                                >  1:991426/1‑150 (MQ=255)
                                         aaaTCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTg                                                                                                              >  2:1367381/1‑150 (MQ=255)
                                             cGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATg                                                                                                          >  2:129423/1‑150 (MQ=255)
                                              gACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGa                                                                                                         >  2:1593195/1‑150 (MQ=255)
                                                ccGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACGCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACg                                                                                                       <  2:1821889/150‑1 (MQ=255)
                                                  gtgtGCGCACCCGCGCGTGCACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGCGCAGCCGTGAATGACGca                                                                                                     >  2:695441/1‑149 (MQ=255)
                                                     tgCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGTACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATc                                                                                                  >  2:1074766/1‑150 (MQ=255)
                                                     tgCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATc                                                                                                  <  1:396901/150‑1 (MQ=255)
                                                      gcgcACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATcc                                                                                                 >  1:1726338/1‑150 (MQ=255)
                                                       cgcACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATccc                                                                                                <  1:158132/150‑1 (MQ=255)
                                                           cccGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTGCGCTTGCACACATTCATTGCCGGAGAGCAGCCGGGAATGCCGCTATCCCTCGc                                                                                            >  2:671789/1‑150 (MQ=255)
                                                               cgcgTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGc                                                                                        >  1:1727365/1‑150 (MQ=255)
                                                                 cgTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAg                                                                                      >  2:1409952/1‑150 (MQ=255)
                                                                      aCCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGaa                                                                                 >  1:224066/1‑150 (MQ=255)
                                                                      aCCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGaa                                                                                 >  2:1504616/1‑150 (MQ=255)
                                                                      aCCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGaa                                                                                 >  2:849941/1‑150 (MQ=255)
                                                                              gAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTa                                                                         >  2:784790/1‑150 (MQ=255)
                                                                              gAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTa                                                                         >  1:715383/1‑150 (MQ=255)
                                                                               aaGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTaa                                                                        <  2:1322388/150‑1 (MQ=255)
                                                                                agagCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAg                                                                       <  1:796362/150‑1 (MQ=255)
                                                                                agagCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAg                                                                       <  2:679675/150‑1 (MQ=255)
                                                                                agagCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAg                                                                       <  2:624891/150‑1 (MQ=255)
                                                                                agagCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAg                                                                       <  1:315822/150‑1 (MQ=255)
                                                                                  agCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGgc                                                                     >  1:1350218/1‑150 (MQ=255)
                                                                                   gCGGAAAATAATCCGCAGTAGTAGATGCCGAAGGGGCGCACCCCACCCGCCGGCAATTCGCCCCATTATGGTGCGCTTGCACACATACAATGCCGGAGAGCAGCCGGGAATGACGCTATCCCTAGCCCGCAGGCGAAAAGCGGTAAGgcg                                                                    >  2:1005339/1‑150 (MQ=255)
                                                                                     ggAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCt                                                                  >  2:812874/1‑150 (MQ=255)
                                                                                      gAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTg                                                                 <  1:1095650/150‑1 (MQ=255)
                                                                                      gAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTg                                                                 <  2:1438556/150‑1 (MQ=255)
                                                                                      gAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTg                                                                 >  1:555028/1‑150 (MQ=255)
                                                                                      gAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTg                                                                 <  1:90816/150‑1 (MQ=255)
                                                                                        aaaTAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAc                                                               <  2:1670838/150‑1 (MQ=255)
                                                                                        aaaTAATCCGCAGTCGTAGATGCCGGAGGGGCACACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAg                                                                                      >  2:1367415/1‑127 (MQ=255)
                                                                                           taatCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAcgcc                                                            <  1:414445/150‑1 (MQ=255)
                                                                                               ccGCAGTCGTAGATGCCGGAGGGGCGCACCCCACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTAGCCCTCGCCCGCCTGCGAAAACCCGTAAGGCGCTGAcgccgcga                                                        >  2:1180691/1‑148 (MQ=255)
                                                                                                 gCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTAtt                                                      <  2:396043/150‑1 (MQ=255)
                                                                                                  cAGTCGTAGATGCCGGAGGGGCGCATCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCCCACAGTCAATGCCGGAGAGCAGCCGTGAATGCCGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTa                                                     >  1:1593487/1‑150 (MQ=255)
                                                                                                   aGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAg                                                    <  1:343639/150‑1 (MQ=255)
                                                                                                      cGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGaaa                                                 <  1:1174058/150‑1 (MQ=255)
                                                                                                        tAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATc                                               <  1:1200531/150‑1 (MQ=255)
                                                                                                            tGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGCCGCCGCTATTAGAAATCCGCa                                           >  1:1672760/1‑150 (MQ=255)
                                                                                                             gCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGcca                                          >  2:89587/1‑148 (MQ=255)
                                                                                                             gCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCaa                                          >  1:964570/1‑150 (MQ=255)
                                                                                                              ccGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAAc                                         <  2:1144071/150‑1 (MQ=255)
                                                                                                              ccGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAAc                                         >  2:1250182/1‑150 (MQ=255)
                                                                                                                ggAGGGGCGCACCACACCCGCCGGCAATTAGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCt                                       >  2:1481498/1‑150 (MQ=255)
                                                                                                                     ggCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCa                                  >  1:648984/1‑150 (MQ=255)
                                                                                                                      gcgcACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCaa                                 >  2:642882/1‑150 (MQ=255)
                                                                                                                        gcACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCTAAAACCCGTAAGGCGCTGACGCCGCGGTTAGAAATCCGCAACCTGACCAAAt                               >  2:244736/1‑150 (MQ=255)
                                                                                                                          accacACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATcc                             <  2:1726338/150‑1 (MQ=255)
                                                                                                                               acCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACCTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGa                        <  2:560116/150‑1 (MQ=255)
                                                                                                                               acCCGCCGGCAATTCGCACCAGTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGCCGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGa                        >  1:633397/1‑150 (MQ=255)
                                                                                                                                cccGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGAt                       >  2:1216446/1‑150 (MQ=255)
                                                                                                                                  cgccgTCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATgg                     <  1:106340/150‑1 (MQ=255)
                                                                                                                                  cgccgGCAATTCGCACCATTATGGTGCGATTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGCCGCCGCGATTAGAAATCCGCAACCTGACCATATCCTACGATgg                     >  1:1596873/1‑150 (MQ=255)
                                                                                                                                     cgGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCa                  >  1:1103673/1‑150 (MQ=255)
                                                                                                                                        gcaagcGCACCATAATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACACTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACa               >  2:1532174/6‑150 (MQ=255)
                                                                                                                                             cGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCACAACCTGACCAAATCCTACGATGGTCAACATGCgg          >  1:364867/1‑150 (MQ=255)
                                                                                                                                              gCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGt         >  1:614237/1‑150 (MQ=255)
                                                                                                                                               cACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTg        <  2:478387/150‑1 (MQ=255)
                                                                                                                                                 ccATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGga      <  2:1145937/150‑1 (MQ=255)
                                                                                                                                                    ttATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGgatga   <  2:1590019/150‑1 (MQ=255)
                                                                                                                                                    ttATGGTGCGCTTGCACACATACAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGCCCAAATCCTACGATGGTCAACATGCGGTGgatga   >  1:981807/1‑150 (MQ=255)
                                                                                                                                                     tATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTCAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGCCCAAATCCTACGATGGTCAACATGCGGTGgatgat  <  1:55565/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTTCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGAT  >  NC_000913/894803‑895101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: