Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 900,205 A→T 5.3% L124Q (CTG→CAG)  artJ ← arginine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913900,2050AT 5.3% 305.6 / 4.4 104L124Q (CTG→CAG) artJarginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (50/47);  new base T (4/2);  total (54/50)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CACCAAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGAC  >  NC_000913/900056‑900354
                                                                                                                                                     |                                                                                                                                                     
cACCAAATACCCCATCAATACGCCCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCa                                                                                                                                                       >  2:152983/1‑150 (MQ=255)
cACCAAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCa                                                                                                                                                       >  2:526919/1‑150 (MQ=255)
cACCAAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCa                                                                                                                                                       <  2:1797642/150‑1 (MQ=255)
 aCCAAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAg                                                                                                                                                      <  2:1333762/150‑1 (MQ=255)
    aaaTACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATc                                                                                                                                                   <  2:1497264/150‑1 (MQ=255)
      aTACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCgg                                                                                                                                                 <  1:1707780/150‑1 (MQ=255)
        aCCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCa                                                                                                                                               <  1:1175480/150‑1 (MQ=255)
        aCCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCa                                                                                                                                               <  2:1339688/150‑1 (MQ=255)
        aCCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCa                                                                                                                                               <  2:1556973/150‑1 (MQ=255)
         ccccATCAATACGACCATATTTCAGATCGATAAAGGCATACTGATAACTGTCATAAGAGACAGTTTAAACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGTCaa                                                                                                                                              >  2:68556/1‑150 (MQ=255)
           ccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAAc                                                                                                                                            >  1:1621036/1‑150 (MQ=255)
            cATCAAAACGACCAATATTCAGATCGATAAAGGCATTCTGAAAACTGTCATAAGACACACTTATCACTTACGTGTGATGATCCTGCATATATTTCTGGTGAGAAGTACCGTTTTCCTTCCCAATACGTATGCCTTTCAGATCGTCaaaag                                                                                                                                           >  1:1153504/1‑148 (MQ=255)
                aaTACGACCATTTTTCAGATCGATAAAGGCATTCTGATACCTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGtttt                                                                                                                                       <  1:748956/150‑1 (MQ=255)
                        cATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTAt                                                                                                                               >  2:675584/1‑150 (MQ=255)
                         aTTTTTCAGATCGATTAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTTATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATc                                                                                                                              <  1:883034/150‑1 (MQ=255)
                               cAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGCGGGCTGATCCTGAATATATTTCTGCTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                        <  2:1321257/150‑1 (MQ=255)
                                 gATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                      >  1:550154/1‑150 (MQ=255)
                                 gATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                      >  1:1571790/1‑150 (MQ=255)
                                 gATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                      >  1:1237554/1‑150 (MQ=255)
                                 gATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                      <  2:761616/150‑1 (MQ=255)
                                  aTCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGc                                                                                                                     <  1:1052325/150‑1 (MQ=255)
                                  aTCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGc                                                                                                                     <  1:984708/150‑1 (MQ=255)
                                   tCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCa                                                                                                                    >  1:1793383/1‑150 (MQ=255)
                                    cGATAAATGCATTCTGATAACTGTCATAAGATACAGTTTTCACTAACGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTAACGTTTTCCAAACCAAAACTTTTGCATTTCAGATAGGCAAACGTTTTGTAGGTATATTTTTTTGCaa                                                                                                                   >  2:1022893/1‑150 (MQ=255)
                                       tAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCa                                                                                                                >  1:747470/1‑150 (MQ=255)
                                        aaaGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTACGGGTGATGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATACCAATACGTTTGCCTTTCATATCGTAAAACGTTTTGTAGGTATGTTTTTTGACAATCac                                                                                                               >  2:311416/1‑150 (MQ=255)
                                         aaGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCacg                                                                                                              >  2:1398274/1‑150 (MQ=255)
                                         aaGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCacg                                                                                                              >  2:1107021/1‑150 (MQ=255)
                                           ggCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCCTTTTTGGCAATCacgac                                                                                                            >  2:842239/1‑150 (MQ=255)
                                              aTTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGc                                                                                                         >  2:676004/1‑150 (MQ=255)
                                              aTTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGAAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGc                                                                                                         <  1:1543776/150‑1 (MQ=255)
                                                tCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTg                                                                                                       <  2:1565362/150‑1 (MQ=255)
                                                 cTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACTTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGa                                                                                                      >  1:678802/1‑150 (MQ=255)
                                                     tAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGttt                                                                                                  >  1:1312183/1‑150 (MQ=255)
                                                       aCTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTc                                                                                                <  1:1272442/150‑1 (MQ=255)
                                                        cTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCa                                                                                               <  1:1142778/150‑1 (MQ=255)
                                                          gTCATAAGAGACAGTTTTCACATCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCAta                                                                                             <  2:1336393/150‑1 (MQ=255)
                                                            cATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCAtagt                                                                                           >  1:317070/1‑150 (MQ=255)
                                                                     cAGTTTTCACTTCCGGGTGCTGTTCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTCTCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTgg                                                                                  <  1:1275438/150‑1 (MQ=255)
                                                                     cAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTgg                                                                                  <  2:62744/150‑1 (MQ=255)
                                                                     cAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTgg                                                                                  >  1:848861/1‑150 (MQ=255)
                                                                     cAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTgg                                                                                  <  1:453832/150‑1 (MQ=255)
                                                                     cAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGAAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGTCTGAGTTTTCATAGTAGGGCGTgg                                                                                  >  2:1794187/1‑150 (MQ=255)
                                                                      aGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGt                                                                                 <  1:526919/150‑1 (MQ=255)
                                                                         tttCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTTGTaaa                                                                              >  1:220766/1‑150 (MQ=255)
                                                                          ttCACTTCCGGGTGCTGATCCTTAATATATTTCTGGTGCGTATTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAAc                                                                             <  1:1653626/150‑1 (MQ=255)
                                                                             aCTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGAt                                                                          <  2:581454/150‑1 (MQ=255)
                                                                              cTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATa                                                                         >  2:1103276/1‑150 (MQ=255)
                                                                               ttCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATAc                                                                        <  1:1323370/150‑1 (MQ=255)
                                                                                tCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATAcc                                                                       <  1:448904/150‑1 (MQ=255)
                                                                                 ccGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCt                                                                      <  1:328736/150‑1 (MQ=255)
                                                                                      tGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTg                                                                 <  1:1526977/150‑1 (MQ=255)
                                                                                         tGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTa                                                              >  2:1571423/1‑150 (MQ=255)
                                                                                           aaccagAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTATCAGATCGGCAAACGTTTTGTAGTTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCTTGGTAAACGATACCAGTTTGCTACg                                                            >  1:696381/6‑150 (MQ=255)
                                                                                             ccTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCt                                                          >  2:863083/1‑150 (MQ=255)
                                                                                             ccTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCt                                                          >  1:1285573/1‑150 (MQ=255)
                                                                                             ccTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTCCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTTCGcg                                                          >  2:1106574/1‑149 (MQ=255)
                                                                                               tGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTcc                                                        >  2:749809/1‑150 (MQ=255)
                                                                                                gAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCCGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTTAACGAAACCTGTTTGCTACGCTCCg                                                       >  1:209400/1‑150 (MQ=255)
                                                                                                    atatTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGt                                                   <  2:1373324/150‑1 (MQ=255)
                                                                                                     tatTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTg                                                  >  1:1552635/1‑150 (MQ=255)
                                                                                                     tatTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTg                                                  <  2:1621036/150‑1 (MQ=255)
                                                                                                      atTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGa                                                 >  1:1606940/1‑150 (MQ=255)
                                                                                                      atTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGAAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGa                                                 >  2:242396/1‑150 (MQ=255)
                                                                                                          cTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATc                                             >  1:371673/1‑150 (MQ=255)
                                                                                                           tGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATcc                                            <  1:608836/150‑1 (MQ=255)
                                                                                                            gttGCGTATTAACGTTTTTCATACCAATACGTTTCCCTTACAGATCGGAAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTATACGATACCTGTTTGCTACGCTCCGTGGTGATATCCa                                           <  2:1149389/148‑1 (MQ=255)
                                                                                                            ggTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGAGCGGCAAACGTGTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGTTACCTGTTTGCTACGCTCCGGGGTGATATCCa                                           >  2:1725011/1‑150 (MQ=255)
                                                                                                              tGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATa                                         <  1:229900/150‑1 (MQ=255)
                                                                                                                cGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATAcc                                       <  1:19743/150‑1 (MQ=255)
                                                                                                                   agtaCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCCTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGga                                    <  1:842239/150‑1 (MQ=255)
                                                                                                                    gtaCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGgag                                   <  2:1749167/150‑1 (MQ=255)
                                                                                                                       ccGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGAtt                                >  1:5170/1‑150 (MQ=255)
                                                                                                                       ccGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGAtt                                >  1:1569037/1‑150 (MQ=255)
                                                                                                                           tttCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACgg                            <  2:459634/150‑1 (MQ=255)
                                                                                                                            ttCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGc                           >  1:948563/1‑150 (MQ=255)
                                                                                                                             tCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTAATAGTAGGGCGTGTTAAACGATCCCTGTTTTCTACGCTCCGGGGTGATATCCATCCCGGCGATTACGGCg                          >  2:900292/1‑150 (MQ=255)
                                                                                                                               cATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACAGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTc                        <  2:1461017/150‑1 (MQ=255)
                                                                                                                                cctacAAAACGTTTGCCGATCTGAAAGGCAAACGTATTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCa                       >  1:484099/5‑150 (MQ=38)
                                                                                                                                  cccAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCata                     >  1:409346/1‑150 (MQ=255)
                                                                                                                                   acAAAACGTTTGCCGATCTGAAAGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCatat                    >  1:442904/2‑150 (MQ=255)
                                                                                                                                    cAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTATGCTACGCTCCGGGGTGATATCCATACAGGAGATTACGGCGTCATAtt                   >  2:403662/1‑150 (MQ=255)
                                                                                                                                     aaTACGATTGCCTAACAGATCCACAAACGTTTTGTAGGTATCATATTTGGCAATAACTACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACTATAACTGTTTGCTACGCTCCGGGGTGAAATACATACAGGAGATTACGTCGTCATAatt                  >  2:1624747/1‑150 (MQ=255)
                                                                                                                                      aTACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATAtttt                 <  1:927315/150‑1 (MQ=255)
                                                                                                                                       tACGTTTTCCTTTCATATCGGCAAACGTTTTTTAGTTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTTGGGCGTTGTAAACGATACCTGTTTTATACGCTCCGGTGTGATATCCATACCGGATATTACGGCGTCATATTTTc                <  1:414601/150‑1 (MQ=255)
                                                                                                                                       aaCGTTTGCCGATCTGAAAGGCAAACGTATTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTc                >  1:610286/2‑150 (MQ=255)
                                                                                                                                        aggTTTGCCTTTCTGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACTGCTGAGTTTTCATAGTAGGTCGTGGTAAACGATACCTGTTTGCTTCGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCt               <  1:311416/148‑1 (MQ=255)
                                                                                                                                        aCGTTTGCCGATCTGAAAGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCt               >  2:1743180/1‑150 (MQ=255)
                                                                                                                                         cGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGAGGGGGGAGTTTGCATAGTTGGGCGTGGTAAACGCTACCTGGTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTg              >  2:282078/1‑150 (MQ=255)
                                                                                                                                          gTTTGCCGATCTGAAAGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGa             <  1:1743180/150‑1 (MQ=255)
                                                                                                                                               ccTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTc        >  1:571476/1‑150 (MQ=255)
                                                                                                                                                cTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCa       >  1:1005477/1‑150 (MQ=255)
                                                                                                                                                cTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCa       >  1:354271/1‑150 (MQ=255)
                                                                                                                                                cTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCa       <  1:1674351/150‑1 (MQ=255)
                                                                                                                                                 tttCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCTGAGATTACGGCGTCATATTTTCTGAATTTCAg      <  2:691665/150‑1 (MQ=255)
                                                                                                                                                  ttCCGATCGGCAAACGTTTTGTAGGTATATTTTTTTGCAATCACGACGGCTGAGTTTTCATAGGAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGTGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAgg     <  1:608975/150‑1 (MQ=255)
                                                                                                                                                  ttCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAgg     <  2:1205799/150‑1 (MQ=255)
                                                                                                                                                  ttCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAgg     >  2:246235/1‑150 (MQ=255)
                                                                                                                                                     aGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGAc  >  2:500185/1‑150 (MQ=255)
                                                                                                                                                     aGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGAc  >  2:800858/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CACCAAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGAC  >  NC_000913/900056‑900354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑