Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 136,537 A→G 5.4% intergenic (‑73/+33) speE ← / ← yacC spermidine synthase (putrescine aminopropyltransferase)/PulS_OutS family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913136,5370AG 5.4% 273.2 / 4.5 94intergenic (‑73/+33)speE/yacCspermidine synthase (putrescine aminopropyltransferase)/PulS_OutS family protein
Reads supporting (aligned to +/- strand):  ref base A (44/43);  new base G (2/3);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTC  >  NC_000913/136393‑136684
                                                                                                                                                |                                                                                                                                                   
aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                                                                                                                                                <  2:1415291/150‑1 (MQ=255)
aaCGTTATCTACCCCAACGTACTGCCCAAACTGGTCGGGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                                                                                                                                                <  2:306045/150‑1 (MQ=255)
    ttATCTAACGCAAAGTACTGCACAAACTGGTCTTGTAGCGTTTCATGCCACTTTTTTTTTTCTGCCTTGGGTTGATACCTCCTTTGTTAACACCCGTAAATAAAGGGCTCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAata                                                                                                                                            <  2:1419837/150‑1 (MQ=255)
     tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGTGCATCCGCGGTCAatgt                                                                                                                                           <  1:1238562/150‑3 (MQ=255)
      aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGGAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATAtt                                                                                                                                          >  2:1473808/1‑150 (MQ=255)
      aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGTGCATCCGCGGTCAATGtt                                                                                                                                          <  1:713261/150‑1 (MQ=255)
      aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGCCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATAtt                                                                                                                                          >  1:236748/1‑150 (MQ=255)
         tACCGCAAAGTACTGCCCAAACTTGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGTGCATCCGCGGTCAATGttagc                                                                                                                                       <  1:517992/150‑4 (MQ=255)
         tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTTTTAACACCCGTAAAAAAAGGGCGCAACATAATTGCTAACATTGACCGCGGATGCACGGTCAATATTTcg                                                                                                                                       >  1:1343453/1‑149 (MQ=255)
         tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGGAATAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGGCAATATTTCt                                                                                                                                       >  2:679570/1‑150 (MQ=255)
         tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTGTTTTATCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCt                                                                                                                                       <  2:238105/150‑1 (MQ=255)
          aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTa                                                                                                                                      <  1:615713/150‑1 (MQ=255)
          aCCGCAAACTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTATCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTa                                                                                                                                      <  1:72626/150‑1 (MQ=255)
           ccGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTAGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTAc                                                                                                                                     >  2:400810/1‑150 (MQ=255)
                 aGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAg                                                                                                                               <  1:835200/150‑1 (MQ=255)
                  gTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAgg                                                                                                                              <  2:736281/150‑1 (MQ=255)
                  gTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAgg                                                                                                                              <  2:778903/150‑1 (MQ=255)
                      tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTAta                                                                                                                          >  1:452467/1‑149 (MQ=255)
                       gCCCAAACTGGTCGTGTAGCGGTTCATGCCACGGTTTTTTTTCGGCCATGGGGTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCGACATAATAGCTAACAATGACCGCGGATGCCCGGGCAATATTTCGTCAAAAAGGTATCa                                                                                                                         >  2:677447/1‑150 (MQ=255)
                        cccAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATAAg                                                                                                                        >  2:1386668/1‑150 (MQ=255)
                          cAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTAAAAAATCCTTTGTTAACAACCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGACAATATTTCTACAAAAAGGTATCAggg                                                                                                                      >  2:281558/1‑150 (MQ=255)
                                ggTCGTGTAGCGTTTCATTCCACTGTTTTTTTTCTGCCATGGGTTGAAACCTCCTTTGTTAAAACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt                                                                                                                >  2:72655/1‑150 (MQ=255)
                                ggTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTTACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt                                                                                                                >  1:1684094/1‑150 (MQ=255)
                                ggTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt                                                                                                                >  1:414366/1‑150 (MQ=255)
                                ggTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGAAACCTCCATTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGATAACAATGACCCCGGATGCACGGTCAATATTTCAACAAAAAGGTATCCTGGattatt                                                                                                                >  2:1338515/1‑150 (MQ=255)
                                     tgtAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACg                                                                                                           >  1:1382973/1‑150 (MQ=255)
                                     tgtAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTATACAAAAAGGTATCAGGGATTATTTGACg                                                                                                           <  1:208116/150‑1 (MQ=255)
                                        aGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAGGGCGCAACATAAAAGCTAACATTGACCGGGGATGCCCGGGCAAAATTTCATCAAAAAGGTATCATGGATTTTTTGACGTAgg                                                                                                       >  2:1733251/1‑150 (MQ=255)
                                          cGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGc                                                                                                      <  1:662322/150‑1 (MQ=255)
                                           gTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCa                                                                                                     >  1:1681936/1‑150 (MQ=255)
                                           cgttCATGCCACTGTATTTTTTCGGCCATGGGTTGATACCTCCTTTGATAAGACCCGTAAAAAAATGGCGCAACATAATAGCAAACATTGACCGCGGAAGCACGGTCAATATTTCTACAAAAACGTATCAGGGATTATTTGACGTAGGCa                                                                                                     <  1:421575/148‑1 (MQ=255)
                                                aTGCCACTGTTTTTTTTCGGCCATGGTTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAg                                                                                                <  1:1467721/150‑1 (MQ=255)
                                                 tGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAgg                                                                                               <  1:1423284/150‑1 (MQ=255)
                                                   ccACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCt                                                                                             >  2:962251/1‑150 (MQ=255)
                                                     aCTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTa                                                                                           <  2:998146/150‑1 (MQ=255)
                                                      cTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTaa                                                                                          <  1:950783/150‑1 (MQ=255)
                                                       tGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCTGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         <  1:150543/150‑1 (MQ=255)
                                                       tGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         >  1:1060374/1‑150 (MQ=255)
                                                       tGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         <  1:1317428/150‑1 (MQ=255)
                                                       tGTTTTTTTTCGGCCATGGGTTGATACCTCCTATGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCAAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         <  2:818504/150‑1 (MQ=255)
                                                       tGTTATTTTTCTGCCATGGGTTTATACCTCCTTTGTTAACTCCCGTAAAAATAGGGCGCAAAATAATAGATAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                                                                         <  2:1187478/150‑1 (MQ=255)
                                                        tgtttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAgg                                                                                        <  1:855775/148‑1 (MQ=255)
                                                             ttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcg                                                                                   >  2:1165794/1‑150 (MQ=255)
                                                               ttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATATCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcgcg                                                                                 >  1:191559/1‑150 (MQ=255)
                                                                  ggCCATGGGTTGATACCTCCTTTGTTAACACCCGAAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGAAGGATTAAGTAATCGCggtc                                                                              >  1:1158671/1‑148 (MQ=255)
                                                                     cATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAg                                                                           >  2:1459884/1‑150 (MQ=255)
                                                                     cATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAg                                                                           >  2:1042396/1‑150 (MQ=255)
                                                                     cATGGGTTGATACCTCCTTTGTTAACACCAGTAAAAAAAGAGCTCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTCGGCACGCAGTCTTAAGGAATCGCGGGCCAg                                                                           >  1:120131/1‑150 (MQ=255)
                                                                       tGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAggg                                                                         >  2:1400939/1‑150 (MQ=255)
                                                                         ggTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCATCATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGTCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCt                                                                       >  2:1274900/1‑150 (MQ=255)
                                                                         ggTCGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGGCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGTCAAGCAGGGAGAAGGAATCGCGGGCCAGGGCt                                                                       >  2:865304/1‑150 (MQ=255)
                                                                              aTACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATATGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGct                                                                  >  1:436584/1‑149 (MQ=255)
                                                                                 cctcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAttt                                                               >  1:200277/1‑150 (MQ=255)
                                                                                  ctcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtttt                                                              >  1:1812107/1‑150 (MQ=255)
                                                                                   tcatttGTTAACACACGTAAAAAAAGGGCGCAACATAATCGCTAACATTGACGGCGGATGCACGGTCAATATTTCTACAAATAGGTATCAGGGATTATTTGACGTAGTCAAGCAGTCTTAAGTAATCGCGGGCCAGGGCTTTGCAttttt                                                             <  2:276186/147‑1 (MQ=255)
                                                                                    cctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtttttt                                                            <  2:197578/150‑1 (MQ=255)
                                                                                     ctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTTACATTGACCGCGGATGCACTGTCAATATTTCTACAAAAATGTATCAGGGATTATTTGACGTATGCAAGCAGGCTTAAGGAATCGCGGGCCAGGTCTTTGCATTTTTTa                                                           <  1:1301731/150‑1 (MQ=255)
                                                                                     ctTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTa                                                           >  2:1106294/1‑150 (MQ=255)
                                                                                      tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTTTCCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGTGATTTTTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:3068/150‑1 (MQ=255)
                                                                                      tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:370302/150‑1 (MQ=255)
                                                                                      tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:1378422/150‑1 (MQ=255)
                                                                                      tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:281558/150‑1 (MQ=255)
                                                                                      tttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAg                                                          <  1:1515985/150‑1 (MQ=255)
                                                                                       ttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGc                                                         <  1:400810/150‑1 (MQ=255)
                                                                                       ttGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGc                                                         <  2:1743061/150‑1 (MQ=255)
                                                                                         gTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACGGCGGATGCACGGTCCATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAACCAGGCTTAAGGAATCGCGGGCCAGGGCTTTCCATTTTTTAGCgc                                                       >  2:238655/1‑149 (MQ=255)
                                                                                           tAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACg                                                     >  1:1045730/1‑150 (MQ=255)
                                                                                            aaCACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCCGGGCTTTGCATTTTTTAGCGACgg                                                    >  1:1385873/1‑150 (MQ=255)
                                                                                            aaCACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACgg                                                    >  2:1415059/1‑150 (MQ=255)
                                                                                               acCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTTACCGCGGATGCACGGTCAATATTTCTACAAAAATGAATCAGGGATTATTTGACGTAGGCAAGCAGGATTAAGGAATCGCGGGCCACGGCTTTGCATTTTTTAGCGACGGGaa                                                 >  2:128473/1‑150 (MQ=255)
                                                                                                cccGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaat                                                >  2:1013130/1‑150 (MQ=255)
                                                                                                 ccGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGGTTCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatg                                               <  2:1588084/150‑1 (MQ=255)
                                                                                                  cGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatgc                                              >  1:331403/1‑150 (MQ=255)
                                                                                                   gTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatgcc                                             >  2:660007/1‑150 (MQ=255)
                                                                                                   gTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGaatgcc                                             >  1:1086294/1‑150 (MQ=255)
                                                                                                           aGGGCTCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTCGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGc                                     <  2:708390/150‑1 (MQ=255)
                                                                                                               ctcAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAACAGGAATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGACTCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTgag                                 <  1:708902/148‑1 (MQ=255)
                                                                                                                gcAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTgaga                                >  2:284250/1‑150 (MQ=255)
                                                                                                                 cAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTgagct                               >  1:204193/1‑148 (MQ=255)
                                                                                                                 cAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGAt                               >  1:712193/1‑150 (MQ=255)
                                                                                                                      aaataGCTAACATTGACCTCGTTTGCACGGTCAATATTTCTCCAAAAAGGTATCAGGGATTATATGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCgg                          <  1:952128/149‑1 (MQ=255)
                                                                                                                        ataGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTa                        <  2:1278636/150‑1 (MQ=255)
                                                                                                                          aGCTAACATTGAGCGCGGATGCACGGTCAATATTTCTACTAAAATGTATCAGGTATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGAGTCGGGAATGCCAATGCCGCTGAGATCGCGGTAGc                      <  1:11030/150‑1 (MQ=255)
                                                                                                                          aGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGc                      <  2:1728606/150‑1 (MQ=255)
                                                                                                                          aGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGCGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGc                      <  2:1418285/150‑1 (MQ=255)
                                                                                                                            cTAACATTGACCGCTGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGTCCAGGGCTTTTCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTg                    <  1:1394810/150‑1 (MQ=255)
                                                                                                                              aataTTGACCGTGCATCCGCGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTc                  >  1:761525/4‑150 (MQ=255)
                                                                                                                               ataTTGACCGTGCATCCGCGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCt                 >  1:1305790/3‑150 (MQ=255)
                                                                                                                                   tGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTATCGACGGGAATGCCAATGCCGCTGAGATCTCGGTACCTGTCTTCAc             >  2:1130589/1‑150 (MQ=255)
                                                                                                                                      ccGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGa          >  2:43793/1‑150 (MQ=255)
                                                                                                                                        gcgtaggCACGGGAAATATTTCTATATATACGTATCAGGGATTATTTGACGTAGGCAAGCTGGCTTAAGGACTCGCGGGCGTGTGCTTTGCATTTTTTAGCGACTGGAATGACAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAgg        <  1:285432/144‑1 (MQ=255)
                                                                                                                                         cgGAGGCACGCTCAATATTTCTACAAAACGGAAGCCGGGATTATTGGACGCAGCCAAGCCGCCTAACGGAAGCCCGGGCCAGGGCTTTGCATTTTTTAGCCCCGGCAATGCCAATCCCGCGGAGACCGCGGTAGCTGTCTTCACCGCggg       <  2:592734/150‑1 (MQ=255)
                                                                                                                                             tGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCttt   >  1:441827/1‑150 (MQ=255)
                                                                                                                                              gCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTc  <  2:169439/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGGGAATGCCAATGCCGCTGAGATCGCGGTAGCTGTCTTCACCGAGGGCTTTC  >  NC_000913/136393‑136684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: