Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 993,254 A→G 5.3% intergenic (+20/+23) pepN → / ← ssuB aminopeptidase N/aliphatic sulfonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,2540AG 5.3% 341.6 / 7.2 112intergenic (+20/+23)pepN/ssuBaminopeptidase N/aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (53/53);  new base G (3/3);  total (56/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 115 total aligned reads displayed.

ACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAA  >  NC_000913/993105‑993403
                                                                                                                                                     |                                                                                                                                                     
aCCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCa                                                                                                                                                       >  2:1360937/1‑150 (MQ=255)
      gATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAgcg                                                                                                                                                 >  1:184715/1‑150 (MQ=255)
      gATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAgcg                                                                                                                                                 >  2:159566/1‑150 (MQ=255)
       aTTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGAACTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAgcgc                                                                                                                                                >  2:1534989/1‑150 (MQ=255)
             cTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCAt                                                                                                                                          >  2:324959/1‑150 (MQ=255)
             cTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCAt                                                                                                                                          <  2:317476/150‑1 (MQ=255)
               gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTc                                                                                                                                        <  1:940827/150‑1 (MQ=255)
               gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGGTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTc                                                                                                                                        <  2:220503/150‑1 (MQ=255)
                 aaCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTCgg                                                                                                                                      <  1:1005738/150‑1 (MQ=255)
                       aCGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAtt                                                                                                                                >  1:618666/1‑150 (MQ=255)
                       aCGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAtt                                                                                                                                >  1:1778330/1‑150 (MQ=255)
                           tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCGCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                            <  2:281731/150‑1 (MQ=255)
                            gCCAAACGTCAGTAGAAAATGCGCGCGGCGCTTGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAccc                                                                                                                           <  2:116297/150‑1 (MQ=255)
                              cAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCt                                                                                                                         >  1:173151/1‑150 (MQ=255)
                              cAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAACAGCGCCGCCATTCGGGGAATTTACCCCt                                                                                                                         >  1:607033/1‑150 (MQ=255)
                                  cGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGatt                                                                                                                     >  1:1694257/1‑150 (MQ=255)
                                      cggAGAAAATGCGCGCGGCGATGGAACAGTTGAAAGGGCTGGAAAATCGCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTtctc                                                                                                                 <  2:279558/149‑1 (MQ=255)
                                      aGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTtctc                                                                                                                 <  1:1063679/150‑1 (MQ=255)
                                       ggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                >  2:534367/1‑150 (MQ=255)
                                       ggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                >  1:1406209/1‑150 (MQ=255)
                                          gaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                             >  1:482566/1‑150 (MQ=255)
                                          gaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                             <  2:1739497/150‑1 (MQ=255)
                                          caaaaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAATCACTGGCTTGATAAATAAGCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                             <  1:134833/149‑1 (MQ=255)
                                             aaTGCGCGCGGCGCTGGAACAGTTGAACGGGCTGGAAAAGCTCTCTGGCGAGCTGTACGAGAAGAAAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAg                                                                                                          <  2:1568259/150‑1 (MQ=255)
                                             aaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAg                                                                                                          <  2:1270346/150‑1 (MQ=255)
                                                  gcgcgGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCa                                                                                                     <  2:1009127/150‑1 (MQ=255)
                                                   cgcgGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAg                                                                                                    <  1:1302260/150‑1 (MQ=255)
                                                        cgcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                               <  1:475444/150‑1 (MQ=255)
                                                          cTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacc                                                                                             <  1:473535/150‑1 (MQ=255)
                                                          cTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGAAAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacc                                                                                             <  2:831414/150‑1 (MQ=255)
                                                           tGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacca                                                                                            <  2:61410/150‑1 (MQ=255)
                                                           tGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacca                                                                                            >  2:389750/1‑150 (MQ=255)
                                                            ggAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcaccac                                                                                           >  1:736156/1‑150 (MQ=255)
                                                               aCAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGtt                                                                                        <  1:1048511/150‑1 (MQ=255)
                                                                cAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTg                                                                                       <  1:1463462/150‑1 (MQ=255)
                                                                cAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTg                                                                                       <  1:587129/150‑1 (MQ=255)
                                                                 aGTTTAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGc                                                                                      <  1:346769/150‑1 (MQ=255)
                                                                  gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAACGCACTGGCTTGAGAAATAACCGAATGGCGGCAATAGCGCCGCCATTCCGGCAATTTACCCCTGTTTTCTCCGGCGAGTGTCAGATTCACCACGTTGCa                                                                                     >  1:304036/1‑150 (MQ=255)
                                                                  gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                     >  2:1023200/1‑150 (MQ=255)
                                                                  gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                     >  1:1076292/1‑150 (MQ=255)
                                                                  gTTGAAACGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCAATCGGGCAATTTACAACTGTATTCTCAGGCAAGTTTCAGATTcaacaccataca                                                                                     >  1:154442/1‑144 (MQ=255)
                                                                      aaaGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCAc                                                                                 <  2:510906/150‑1 (MQ=255)
                                                                        agggagggAAAATCTCTCTGGCGATCTGTACGAGAACATTACTAACGCACTGGCTTGATAAATAACCCAATCGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGATTTTCAGTTTCACCACGTTGCATCAccc                                                                               <  2:222651/144‑1 (MQ=255)
                                                                            cTGGAAAATCTCTCTGGCGAGCTGTACGAGAATATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTg                                                                           <  2:266192/150‑1 (MQ=255)
                                                                             tgtaaaaTCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                          <  2:1639761/147‑1 (MQ=255)
                                                                             tGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                          <  1:1522720/150‑1 (MQ=255)
                                                                              ggAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCa                                                                         <  1:834065/150‑1 (MQ=255)
                                                                               gAAAAGCGCTCTGGCGCTCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAACGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCaa                                                                        <  1:328362/150‑1 (MQ=255)
                                                                                aaaaTCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAac                                                                       <  2:1778330/150‑1 (MQ=255)
                                                                                 aaaTCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAaca                                                                      <  1:465672/150‑1 (MQ=255)
                                                                                     ctctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTc                                                                  >  2:1164705/1‑150 (MQ=255)
                                                                                      tctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATAACCCGTTGCAACACCTcc                                                                 >  2:870456/1‑150 (MQ=255)
                                                                                           ggCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTc                                                            >  2:1297992/1‑150 (MQ=255)
                                                                                           ggCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTc                                                            >  2:832967/1‑150 (MQ=255)
                                                                                            gCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATACCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTcc                                                           <  1:566570/150‑1 (MQ=255)
                                                                                                 cTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATATCGCCGCCATTCGTGGAATTTACCCCTGTTTTCTCAGGCGAGATTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTc                                                      >  2:1229266/1‑150 (MQ=255)
                                                                                                  tGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                     >  1:1352035/1‑150 (MQ=255)
                                                                                                  tGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                     >  1:348874/1‑150 (MQ=255)
                                                                                                  tGTACGAGAAGATAACAAAAGCACTGGCTTGTTAACTAACCGACTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                     <  2:189642/150‑1 (MQ=255)
                                                                                                  ggTACGAGACGATAACTCAAGCACTGGCGTGATAACTACCCGACTGGCGGCAATCGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                     <  1:302419/149‑1 (MQ=255)
                                                                                                    tACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGc                                                   <  1:334427/150‑1 (MQ=255)
                                                                                                    tACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGc                                                   >  1:485537/1‑150 (MQ=255)
                                                                                                     aCGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGACTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCt                                                  <  1:1754467/150‑1 (MQ=255)
                                                                                                      cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTa                                                 >  2:1421705/1‑150 (MQ=255)
                                                                                                      cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTa                                                 >  1:1235429/1‑150 (MQ=255)
                                                                                                              tAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCCCCACGTTGAATCACCCGTTGCAACCCCTCCGCTTCCAGCTCCGCTAATCGCAcc                                         >  2:1113000/1‑150 (MQ=255)
                                                                                                              tAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCATCCATTCGGGGAATTTACCCCTGTTTTCTAAGGCGAGTTTCAGATTCACCACGTTGCATCACACTTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCAcc                                         >  2:54557/1‑150 (MQ=255)
                                                                                                                 cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTGTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTTATCGCACCGAc                                      >  1:1728851/1‑150 (MQ=255)
                                                                                                                   aaaGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAccc                                    >  1:1815044/1‑150 (MQ=255)
                                                                                                                    aaGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCt                                   <  1:1360937/150‑1 (MQ=255)
                                                                                                                      gCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTaa                                 >  1:1031152/1‑150 (MQ=255)
                                                                                                                      gCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTaa                                 <  2:560503/150‑1 (MQ=255)
                                                                                                                        aCTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATc                               >  1:953949/1‑150 (MQ=255)
                                                                                                                         cTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCCCCGACCCTAATCg                              >  1:1549182/1‑150 (MQ=255)
                                                                                                                         cTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCg                              <  2:1671257/150‑1 (MQ=255)
                                                                                                                           ggCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAc                            >  2:822355/1‑150 (MQ=255)
                                                                                                                               tGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCgg                        >  2:1194920/1‑150 (MQ=255)
                                                                                                                                gATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGa                       >  1:875817/1‑150 (MQ=255)
                                                                                                                                 ataaataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCATCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGAc                      >  2:1026023/1‑150 (MQ=255)
                                                                                                                                   aaataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAAACGCACCGACCCTAATCGCCGCGGCCgg                    >  1:1349835/1‑150 (MQ=255)
                                                                                                                                     atcaCCGAATGCCGGCAATAGCGCCGCCACTCGGGGAATTTACCCCTGTTTCCTCAGGCGAGTTTCAGATTCACCACCTTGCATCACCCGTTGCAACACCTCCCCTTCCAGCTCCGCTAATCGCTCCGACCCTTATCGACGCGGACgggg                  <  1:282185/147‑1 (MQ=255)
                                                                                                                                       aaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACACCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCGTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGa                >  1:1323336/1‑150 (MQ=255)
                                                                                                                                         ccGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGCCGCGGACGGGGGata              >  1:1449769/1‑150 (MQ=255)
                                                                                                                                         ccGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTACTCGCCCCGACCCGAATCGCCGCGGACGGGGGatt              >  1:952961/1‑149 (MQ=255)
                                                                                                                                           gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc            >  2:1001712/1‑150 (MQ=255)
                                                                                                                                           gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc            <  2:761105/150‑1 (MQ=255)
                                                                                                                                           gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCGCAGGCGCGTGTCAGATTCCCCCCGTTGCATCACCCGGTGCCACACCTCCGCTTCCAGCGCCGCTAATCGCACCGCCCCGAATCGACGCGGTCGGGGGAtctc            >  1:517483/1‑150 (MQ=255)
                                                                                                                                            aaTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCCCCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCCCCGACCCTAATCGACGCGGACGGGGGatatac           >  1:921488/1‑148 (MQ=255)
                                                                                                                                            aaTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCGAATCGCACCGACCCTAATCGACGCGGACGGGGGATATcc           >  1:741081/1‑150 (MQ=255)
                                                                                                                                            aaTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATcc           <  2:368308/150‑1 (MQ=255)
                                                                                                                                             aTGGCGTCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGCCCCTAATCGACGCGGACGGGGGATATCCa          <  1:558283/150‑1 (MQ=255)
                                                                                                                                               ggcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACt        <  2:399615/150‑1 (MQ=255)
                                                                                                                                               ggcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACt        <  2:599009/150‑1 (MQ=255)
                                                                                                                                                gcggcAATAGCGCCGCCATTCGTGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGATGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGTGGATATCCACTg       <  1:81751/150‑1 (MQ=255)
                                                                                                                                                gcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCCCCCGTTGCAACACCTCCGCTTCCAGCTCCGATAATCGCACCGACACTAATCGAGGCGGCCGGGGGAGATGCACTg       >  2:902788/1‑150 (MQ=255)
                                                                                                                                                 cggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGt      <  2:1406209/150‑1 (MQ=255)
                                                                                                                                                   gcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCa    <  2:517648/150‑1 (MQ=255)
                                                                                                                                                   gcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCa    <  1:1049366/150‑1 (MQ=255)
                                                                                                                                                     aaTAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCaaa  <  2:1028541/150‑1 (MQ=255)
                                                                                                                                                     aaTAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCaaa  <  1:114310/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAA  >  NC_000913/993105‑993403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: