Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 993,259 C→T 5.4% intergenic (+25/+18) pepN → / ← ssuB aminopeptidase N/aliphatic sulfonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,2590CT 5.4% 353.0 / 5.9 111intergenic (+25/+18)pepN/ssuBaminopeptidase N/aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (49/56);  new base T (3/3);  total (52/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-01

Only 100 of 116 total aligned reads displayed.

GATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATCCAG  >  NC_000913/993111‑993408
                                                                                                                                                    |                                                                                                                                                     
gATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAgcg                                                                                                                                                      >  1:184715/1‑150 (MQ=255)
gATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAgcg                                                                                                                                                      >  2:159566/1‑150 (MQ=255)
       cTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCAt                                                                                                                                               >  2:324959/1‑150 (MQ=255)
         gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTc                                                                                                                                             <  1:940827/150‑1 (MQ=255)
         gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTc                                                                                                                                             <  1:1569780/150‑1 (MQ=255)
         gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGGTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTc                                                                                                                                             <  2:220503/150‑1 (MQ=255)
           aaCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTCgg                                                                                                                                           <  1:1005738/150‑1 (MQ=255)
                 aCGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAtt                                                                                                                                     >  1:618666/1‑150 (MQ=255)
                  cGATGCCAAACGTCAGGAGAAAATGCGCGCCGCGCTGGAACAGTTGAAAGGGCTGGAAACTCTCTCGGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATACCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAttt                                                                                                                                    >  1:244722/1‑150 (MQ=255)
                     tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCGCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                                 <  2:281731/150‑1 (MQ=255)
                      gCCAAACGTCAGTAGAAAATGCGCGCGGCGCTTGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAccc                                                                                                                                <  2:116297/150‑1 (MQ=255)
                        cAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCt                                                                                                                              >  1:173151/1‑150 (MQ=255)
                            cGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGatt                                                                                                                          >  1:1694257/1‑150 (MQ=255)
                                cggAGAAAATGCGCGCGGCGATGGAACAGTTGAAAGGGCTGGAAAATCGCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTtctc                                                                                                                      <  2:279558/149‑1 (MQ=255)
                                aGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTtctc                                                                                                                      <  1:1063679/150‑1 (MQ=255)
                                 ggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                     >  1:1406209/1‑150 (MQ=255)
                                 ggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                     >  2:534367/1‑150 (MQ=255)
                                    gaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                                  <  2:1739497/150‑1 (MQ=255)
                                    caaaaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAATCACTGGCTTGATAAATAAGCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                                  <  1:134833/149‑1 (MQ=255)
                                       aaTGCGCGCGGCGCTGGAACAGTTGAACGGGCTGGAAAAGCTCTCTGGCGAGCTGTACGAGAAGAAAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAg                                                                                                               <  2:1568259/150‑1 (MQ=255)
                                       aaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAg                                                                                                               <  2:1270346/150‑1 (MQ=255)
                                            gcgcgGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCa                                                                                                          <  2:1009127/150‑1 (MQ=255)
                                             cgcgGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAg                                                                                                         <  1:1302260/150‑1 (MQ=255)
                                                  cgcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                                    <  1:475444/150‑1 (MQ=255)
                                                    cTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacc                                                                                                  >  1:517648/1‑150 (MQ=255)
                                                    cTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacc                                                                                                  <  1:473535/150‑1 (MQ=255)
                                                    cTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGAAAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacc                                                                                                  <  2:831414/150‑1 (MQ=255)
                                                     tGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacca                                                                                                 >  2:389750/1‑150 (MQ=255)
                                                     tGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacca                                                                                                 <  2:61410/150‑1 (MQ=255)
                                                      ggAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcaccac                                                                                                >  1:736156/1‑150 (MQ=255)
                                                         aCAGTTTAAAGGGCTGGAAACTCTCTCTGGCGATCTGTACGAGTAGATAACTAAAGCACTGGCTTGATAAATATCCGAATGGGGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGtt                                                                                             <  2:1157712/150‑1 (MQ=255)
                                                         aCAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGtt                                                                                             <  1:1048511/150‑1 (MQ=255)
                                                          cAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTg                                                                                            <  1:1463462/150‑1 (MQ=255)
                                                          cAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTg                                                                                            <  1:587129/150‑1 (MQ=255)
                                                           aGTTTAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGc                                                                                           <  1:346769/150‑1 (MQ=255)
                                                            gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAACGCACTGGCTTGAGAAATAACCGAATGGCGGCAATAGCGCCGCCATTCCGGCAATTTACCCCTGTTTTCTCCGGCGAGTGTCAGATTCACCACGTTGCa                                                                                          >  1:304036/1‑150 (MQ=255)
                                                            gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                          >  1:1076292/1‑150 (MQ=255)
                                                            gTTGAAACGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCAATCGGGCAATTTACAACTGTATTCTCAGGCAAGTTTCAGATTcaacaccataca                                                                                          >  1:154442/1‑144 (MQ=255)
                                                               gAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCa                                                                                       >  2:1676809/1‑150 (MQ=255)
                                                                  agggagggAAAATCTCTCTGGCGATCTGTACGAGAACATTACTAACGCACTGGCTTGATAAATAACCCAATCGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGATTTTCAGTTTCACCACGTTGCATCAccc                                                                                    <  2:222651/144‑1 (MQ=255)
                                                                      cTGGAAAATCTCTCTGGCGAGCTGTACGAGAATATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTg                                                                                <  2:266192/150‑1 (MQ=255)
                                                                       tgtaaaaTCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                               <  2:1639761/147‑1 (MQ=255)
                                                                       tGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                               <  1:1522720/150‑1 (MQ=255)
                                                                        ggAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCa                                                                              <  1:429777/150‑1 (MQ=255)
                                                                        ggAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCa                                                                              <  1:834065/150‑1 (MQ=255)
                                                                         gAAAAGCGCTCTGGCGCTCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAACGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCaa                                                                             <  1:328362/150‑1 (MQ=255)
                                                                           aaaTCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAaca                                                                           <  1:465672/150‑1 (MQ=255)
                                                                               ctctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTc                                                                       >  2:1164705/1‑150 (MQ=255)
                                                                               ctctctGGCGATCTGTACGAGAAGATAAATAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTCCCACTGTTTTCTCAGGCGAGTTTCAGATTCACCAAGTTGCATCGCCCGTTGCCACACCTc                                                                       >  2:278504/1‑150 (MQ=255)
                                                                                tctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATAACCCGTTGCAACACCTcc                                                                      >  2:870456/1‑150 (MQ=255)
                                                                                     ggCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTc                                                                 >  2:1297992/1‑150 (MQ=255)
                                                                                     ggCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTc                                                                 >  2:832967/1‑150 (MQ=255)
                                                                                      gCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATACCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTcc                                                                <  1:566570/150‑1 (MQ=255)
                                                                                       cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                               <  2:1383499/150‑1 (MQ=255)
                                                                                        gATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACCCCTCCGCTTCCAg                                                              >  1:1361660/1‑150 (MQ=255)
                                                                                           cTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATATCGCCGCCATTCGTGGAATTTACCCCTGTTTTCTCAGGCGAGATTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTc                                                           >  2:1229266/1‑150 (MQ=255)
                                                                                            tGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                          >  1:348874/1‑150 (MQ=255)
                                                                                            tGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                          >  1:1352035/1‑150 (MQ=255)
                                                                                            tGTACGAGAAGATAACAAAAGCACTGGCTTGTTAACTAACCGACTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                          <  2:189642/150‑1 (MQ=255)
                                                                                            ggTACGAGACGATAACTCAAGCACTGGCGTGATAACTACCCGACTGGCGGCAATCGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                          <  1:302419/149‑1 (MQ=255)
                                                                                             gtgcGAGAAGATACCTACAGCAGTGGCTTGATAAATAACCGAAGGGCGTCATTTGCGCCGACATTCTGGGAATATACCCCTGTTTTCTCAGGCGTGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCg                                                         <  1:75403/147‑1 (MQ=255)
                                                                                              tACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGc                                                        >  1:485537/1‑150 (MQ=255)
                                                                                               aCGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGACTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCt                                                       <  1:1754467/150‑1 (MQ=255)
                                                                                                cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTa                                                      >  2:1421705/1‑150 (MQ=255)
                                                                                                cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTa                                                      >  1:1235429/1‑150 (MQ=255)
                                                                                                        tAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCCCCACGTTGAATCACCCGTTGCAACCCCTCCGCTTCCAGCTCCGCTAATCGCAcc                                              >  2:1113000/1‑150 (MQ=255)
                                                                                                        tAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCATCCATTCGGGGAATTTACCCCTGTTTTCTAAGGCGAGTTTCAGATTCACCACGTTGCATCACACTTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCAcc                                              >  2:54557/1‑150 (MQ=255)
                                                                                                           cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTGTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTTATCGCACCGAc                                           >  1:1728851/1‑150 (MQ=255)
                                                                                                             aaaGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAccc                                         >  1:1815044/1‑150 (MQ=255)
                                                                                                                gCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTaa                                      >  1:1031152/1‑150 (MQ=255)
                                                                                                                gCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTaa                                      <  2:560503/150‑1 (MQ=255)
                                                                                                                  aCTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATc                                    >  1:953949/1‑150 (MQ=255)
                                                                                                                   cTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCg                                   <  2:1671257/150‑1 (MQ=255)
                                                                                                                     ggCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAc                                 >  2:822355/1‑150 (MQ=255)
                                                                                                                          gATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGa                            >  2:478290/1‑150 (MQ=255)
                                                                                                                           ataaataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCATCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGAc                           >  2:1026023/1‑150 (MQ=255)
                                                                                                                             aaataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAAACGCACCGACCCTAATCGCCGCGGCCgg                         >  1:1349835/1‑150 (MQ=255)
                                                                                                                               atcaCCGAATGCCGGCAATAGCGCCGCCACTCGGGGAATTTACCCCTGTTTCCTCAGGCGAGTTTCAGATTCACCACCTTGCATCACCCGTTGCAACACCTCCCCTTCCAGCTCCGCTAATCGCTCCGACCCTTATCGACGCGGACgggg                       <  1:282185/147‑1 (MQ=255)
                                                                                                                                 aaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACACCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCGTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGa                     >  1:1323336/1‑150 (MQ=255)
                                                                                                                                   ccGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGCCGCGGACGGGGGata                   >  1:1449769/1‑150 (MQ=255)
                                                                                                                                   ccGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTACTCGCCCCGACCCGAATCGCCGCGGACGGGGGatt                   >  1:952961/1‑149 (MQ=255)
                                                                                                                                     gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc                 <  2:761105/150‑1 (MQ=255)
                                                                                                                                     gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc                 >  2:1001712/1‑150 (MQ=255)
                                                                                                                                     gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCGCAGGCGCGTGTCAGATTCCCCCCGTTGCATCACCCGGTGCCACACCTCCGCTTCCAGCGCCGCTAATCGCACCGCCCCGAATCGACGCGGTCGGGGGAtctc                 >  1:517483/1‑150 (MQ=255)
                                                                                                                                      aaTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCCCCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCCCCGACCCTAATCGACGCGGACGGGGGatatac                >  1:921488/1‑148 (MQ=255)
                                                                                                                                      aaTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCGAATCGCACCGACCCTAATCGACGCGGACGGGGGATATcc                >  1:741081/1‑150 (MQ=255)
                                                                                                                                      aaTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATcc                <  2:368308/150‑1 (MQ=255)
                                                                                                                                       aTGGCGTCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGCCCCTAATCGACGCGGACGGGGGATATCCa               <  1:558283/150‑1 (MQ=255)
                                                                                                                                         ggcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACt             <  2:399615/150‑1 (MQ=255)
                                                                                                                                         ggcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACt             <  2:599009/150‑1 (MQ=255)
                                                                                                                                          gcggcAATAGCGCCGCCATTCGTGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGATGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGTGGATATCCACTg            <  1:81751/150‑1 (MQ=255)
                                                                                                                                          gcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCCCCCGTTGCAACACCTCCGCTTCCAGCTCCGATAATCGCACCGACACTAATCGAGGCGGCCGGGGGAGATGCACTg            >  2:902788/1‑150 (MQ=255)
                                                                                                                                           cggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGt           <  2:1406209/150‑1 (MQ=255)
                                                                                                                                             gcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCa         <  2:517648/150‑1 (MQ=255)
                                                                                                                                             gcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCa         <  1:965095/150‑1 (MQ=255)
                                                                                                                                             gcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCa         <  1:1049366/150‑1 (MQ=255)
                                                                                                                                               aaTAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCaaa       <  2:1028541/150‑1 (MQ=255)
                                                                                                                                               aaTAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCaaa       <  1:114310/150‑1 (MQ=255)
                                                                                                                                                  aGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATcc    <  2:482566/150‑1 (MQ=255)
                                                                                                                                                    cgccgcCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATCCAg  <  1:1674011/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATCCAG  >  NC_000913/993111‑993408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: