Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,101,557 G→C 5.9% P43R (CCA→CGA) ‡ csgG ← curli production assembly/transport outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,101,5570GC 5.9% 318.0 / 7.6 99P43R (CCA→CGA) ‡csgGcurli production assembly/transport outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (42/51);  new base C (4/2);  total (46/53)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 102 total aligned reads displayed.

ACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGC  >  NC_000913/1101408‑1101704
                                                                                                                                                     |                                                                                                                                                   
aCCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTg                                                                                                                                                     <  1:1523141/150‑1 (MQ=255)
aCCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTg                                                                                                                                                     <  2:65998/150‑1 (MQ=255)
   aGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCa                                                                                                                                                  <  2:855777/150‑1 (MQ=255)
     agagaATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTAGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGa                                                                                                                                                >  2:1269383/2‑150 (MQ=255)
        gaATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATgg                                                                                                                                             <  2:1234179/150‑1 (MQ=255)
         aaTCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATggg                                                                                                                                            <  1:837643/150‑1 (MQ=255)
         aaTCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATggg                                                                                                                                            <  1:986044/150‑1 (MQ=255)
          aTCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACACGTCGGCGCTGGCAGATGGGt                                                                                                                                           >  2:415937/1‑150 (MQ=255)
          aTCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGATACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGt                                                                                                                                           <  1:1300014/150‑1 (MQ=255)
             tttCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTATGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCaa                                                                                                                                        >  2:657575/1‑150 (MQ=255)
                cAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGACCAGCAGTGGTGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATc                                                                                                                                     >  1:1550269/1‑150 (MQ=255)
                 cgTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGGCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCGGGCAGAGGGGTCAAATCt                                                                                                                                    <  2:1587700/149‑1 (MQ=255)
                    gCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTg                                                                                                                                 <  1:37307/150‑1 (MQ=255)
                       gTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAg                                                                                                                              <  2:651859/150‑1 (MQ=255)
                        tGACCAGCATTGCCGGGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGc                                                                                                                             <  2:1166479/150‑1 (MQ=255)
                        ggACCCGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGCTTAAATTGCCCGGTTTCGTCCTGAATGTTGAATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGc                                                                                                                             <  1:17470/149‑1 (MQ=255)
                          aCCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGtttcgg                                                                                                                           <  1:1668719/150‑6 (MQ=25)
                           ccAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGctct                                                                                                                          >  2:1708837/1‑150 (MQ=255)
                            cAGCATTGCCGGGGCGCTGTGCGGAACAGCAGGGTAGAAGTTACTTGCCGTGTTGGGTTTAAATTGCCCGGTTTCGTCCTTAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTg                                                                                                                         <  2:1327147/150‑1 (MQ=255)
                              gCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGGAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAg                                                                                                                       <  2:969330/150‑1 (MQ=255)
                              ccATTGCCGTGGCGCTTTTCGGAACAGCAGTGGATAAGTTACTTGCCTGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTTTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAg                                                                                                                       <  2:893785/149‑1 (MQ=255)
                               cATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGc                                                                                                                      >  2:1696800/1‑150 (MQ=255)
                                aTTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGCAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCa                                                                                                                     <  1:682052/150‑1 (MQ=255)
                                aTTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCTGATGGGTCAAATCTTTGTAGCTCTGAGCa                                                                                                                     >  1:646879/1‑150 (MQ=255)
                                 tggCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCAc                                                                                                                    <  2:1208305/148‑1 (MQ=255)
                                        ggCGCTTTGAGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGTTTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCAt                                                                                                             <  2:904194/150‑1 (MQ=255)
                                          cgcTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTa                                                                                                           >  2:1522331/1‑150 (MQ=255)
                                          cgcTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCAGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCAGTa                                                                                                           >  1:122855/1‑150 (MQ=255)
                                           gcTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTaa                                                                                                          <  2:797943/150‑1 (MQ=255)
                                            cTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAAt                                                                                                         >  2:1182068/1‑150 (MQ=255)
                                               tGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTc                                                                                                      <  2:514598/150‑1 (MQ=255)
                                               tGCGGAACAGCAGGGGAGAAGGTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTc                                                                                                      <  2:238089/150‑1 (MQ=255)
                                                   gAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTc                                                                                                  <  2:557815/150‑1 (MQ=255)
                                                    aaCAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCt                                                                                                 >  2:806815/1‑150 (MQ=255)
                                                    aaCAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCt                                                                                                 >  2:132039/1‑150 (MQ=255)
                                                        gcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggcg                                                                                             >  2:1597345/1‑150 (MQ=255)
                                                             ggAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACACGTCGGCGCTGGCAGATTGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttc                                                                                        >  2:876551/1‑150 (MQ=255)
                                                              gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                       >  2:1508227/1‑150 (MQ=255)
                                                              gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                       >  2:1333922/1‑150 (MQ=255)
                                                                gaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCttt                                                                                     <  2:991440/150‑1 (MQ=255)
                                                                 aaGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTa                                                                                    <  2:1280349/150‑1 (MQ=255)
                                                                  aGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATATCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAg                                                                                   >  2:411197/1‑150 (MQ=255)
                                                                       cTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggg                                                                              >  2:1612325/1‑150 (MQ=255)
                                                                         tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                            >  1:523662/1‑150 (MQ=255)
                                                                         tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                            <  2:1412345/150‑1 (MQ=255)
                                                                          gCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggcg                                                                           <  1:1538498/150‑1 (MQ=255)
                                                                           ccGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggcgg                                                                          <  2:41825/150‑1 (MQ=255)
                                                                            cGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGt                                                                         >  2:1009906/1‑150 (MQ=255)
                                                                            cGGGTAGGGTTTAAATTGCCCGGTATCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGAAGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAAAGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGt                                                                         >  2:1361199/1‑150 (MQ=255)
                                                                               gTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                                      >  1:460883/1‑150 (MQ=255)
                                                                               gTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                                      <  1:415937/150‑1 (MQ=255)
                                                                               gTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                                      <  1:547918/150‑1 (MQ=255)
                                                                                    gTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATc                                                                 >  1:1676462/1‑150 (MQ=255)
                                                                                        aaaTTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCt                                                             >  1:521780/1‑150 (MQ=255)
                                                                                           ttGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTCCCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAg                                                          <  1:889384/150‑1 (MQ=255)
                                                                                           ttGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAg                                                          <  1:657575/150‑1 (MQ=255)
                                                                                           ttGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATGTTACCCGTCGGCGCGGGCAGATGGGGCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAg                                                          <  2:511673/150‑1 (MQ=255)
                                                                                             gCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTa                                                        <  1:151667/150‑1 (MQ=255)
                                                                                               ccGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAAc                                                      >  2:697573/1‑150 (MQ=255)
                                                                                                   tttCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGa                                                  <  2:884999/150‑1 (MQ=255)
                                                                                                    ttCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGAc                                                 >  1:293003/1‑150 (MQ=255)
                                                                                                     tCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGGCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACg                                                <  1:1619204/150‑1 (MQ=255)
                                                                                                      cGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGTCGGTTAAGCATCCGCTCAGTAACATGACgg                                               >  2:660893/1‑150 (MQ=255)
                                                                                                      cGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACgg                                               <  2:1444870/150‑1 (MQ=255)
                                                                                                       gTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGc                                              >  1:157266/1‑150 (MQ=255)
                                                                                                         ccTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCaa                                            >  1:616569/1‑150 (MQ=255)
                                                                                                          cTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGAGGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGGCTGGCGGCTTCTTTAGGCGGGGCGGGTAAGCATCCGCTCAGTAACATGACGGCAAc                                           <  2:55642/150‑1 (MQ=255)
                                                                                                           tGAATGGTGTATACCGAAGCAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAAcc                                          <  1:1550530/150‑1 (MQ=255)
                                                                                                               tgttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGCGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCaaaa                                      >  2:1636478/1‑150 (MQ=255)
                                                                                                                gttgtATACCGAAACAAACATTTAACCCCTCGCCGCTCGCAGATGGTTCAAATCTTTGTAGCTCTGAGCACCAGGCAATAATGTCGGGCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAg                                     <  2:89902/150‑1 (MQ=255)
                                                                                                                 ttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGGAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGa                                    <  1:1822735/150‑1 (MQ=255)
                                                                                                                 ttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGa                                    <  2:1223814/150‑1 (MQ=255)
                                                                                                                   gtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTTATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTGAGTCACATGACGGCAACCAAAAGaaa                                  >  1:280274/1‑150 (MQ=255)
                                                                                                                   gtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGCCGGCAACCAAAAGaaa                                  >  1:930257/1‑150 (MQ=255)
                                                                                                                       aCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAg                              >  2:19211/1‑150 (MQ=255)
                                                                                                                        ccGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCACCCAAAAGAAATAAgc                             >  1:482085/1‑150 (MQ=255)
                                                                                                                        ccGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                             <  1:1305214/150‑1 (MQ=255)
                                                                                                                         cGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgcg                            <  1:1617043/150‑1 (MQ=255)
                                                                                                                          gctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGTGGCGGTTAAGCATCCGCTCAGTAACATGACTGCAACCAAAAGAAATAAtcgc                           >  2:1543931/4‑150 (MQ=25)
                                                                                                                          gctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgcgc                           >  2:622880/4‑150 (MQ=25)
                                                                                                                           ctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCt                          >  1:1431434/3‑150 (MQ=37)
                                                                                                                           aaaCAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCt                          >  1:124349/1‑150 (MQ=255)
                                                                                                                           aaaCAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCt                          <  1:1311529/150‑1 (MQ=255)
                                                                                                                            taCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGATg                         >  2:158395/2‑150 (MQ=37)
                                                                                                                              cAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                       >  1:1089174/1‑150 (MQ=255)
                                                                                                                               aaaGATTTTACCCGTCGGCGCTGGCAGATGTGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCAt                      <  1:192883/150‑1 (MQ=255)
                                                                                                                               aaaGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCAt                      >  1:401711/1‑150 (MQ=255)
                                                                                                                               aaaGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCAt                      <  1:1241709/150‑1 (MQ=255)
                                                                                                                                    ttttACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTATGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATACGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGatta                 >  1:945260/1‑150 (MQ=255)
                                                                                                                                        aCCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTAtttt             <  1:1018684/150‑1 (MQ=255)
                                                                                                                                         cccGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTc            <  1:411197/150‑1 (MQ=255)
                                                                                                                                         cccGTCAGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGACGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTc            >  1:263082/1‑150 (MQ=255)
                                                                                                                                          ccGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTGTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACAAGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTcc           <  2:366353/150‑1 (MQ=255)
                                                                                                                                          ccGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTcc           <  1:1708837/150‑1 (MQ=255)
                                                                                                                                            gTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCtt         <  1:1269383/150‑1 (MQ=255)
                                                                                                                                             tCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTa        <  2:1676462/150‑1 (MQ=255)
                                                                                                                                                gcgcTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGa     >  1:1191291/1‑150 (MQ=255)
                                                                                                                                                 cgcTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGaa    <  2:523662/150‑1 (MQ=255)
                                                                                                                                                   cgggCAGCTGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCACTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGc  <  2:130427/148‑1 (MQ=255)
                                                                                                                                                   cTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGc  >  1:1039249/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGC  >  NC_000913/1101408‑1101704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: