Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,104,931 G→T 5.7% intergenic (+29/‑30) csgA → / → csgC curlin subunit, amyloid curli fibers, cryptic/curli assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,104,9310GT 5.7% 395.8 / 6.1 134intergenic (+29/‑30)csgA/csgCcurlin subunit, amyloid curli fibers, cryptic/curli assembly protein
Reads supporting (aligned to +/- strand):  ref base G (58/67);  new base T (3/6);  total (61/73)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

ATGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGA  >  NC_000913/1104783‑1105080
                                                                                                                                                    |                                                                                                                                                      
aTGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGgcgc                                                                                                                                                       >  1:1528429/1‑150 (MQ=255)
    cGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGc                                                                                                                                                   <  1:916084/150‑1 (MQ=255)
      gTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGccc                                                                                                                                                 <  1:1823969/150‑1 (MQ=255)
          aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtt                                                                                                                                            >  2:169837/1‑150 (MQ=255)
          aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGtt                                                                                                                                            >  2:56974/1‑150 (MQ=255)
          aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGACCTGtt                                                                                                                                            >  1:658219/1‑150 (MQ=255)
           aCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttt                                                                                                                                           >  1:321648/1‑150 (MQ=255)
            cAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACTGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttc                                                                                                                                          <  1:391189/150‑2 (MQ=255)
            cAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttc                                                                                                                                          <  2:975380/150‑2 (MQ=255)
            cAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCAGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttc                                                                                                                                          <  1:459792/150‑2 (MQ=255)
              gTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTGtttctg                                                                                                                                        <  2:1342194/150‑4 (MQ=255)
               ttCGGTGGTTGCAAAGTTGCTTCAGTTTAACAGACTTCATATAAATACTCCTTAAACGTGACTCATTTTGGCTTTGGTAACAAATCGACATATCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTCAAGCCCTGttttttt                                                                                                                                       <  2:1288252/150‑1 (MQ=255)
               ttCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGttttttt                                                                                                                                       <  1:64461/150‑1 (MQ=255)
                 gggtgggGGCAACGGTGCTGCATTTTACCAGACTGAATCTAACTCCTCAGTCAAAGTGACGCAGGTTGGCGTTGGTAACAACGCGACCGCTCATCAGTACTAATACAGCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTc                                                                                                                                     <  2:1003612/149‑1 (MQ=255)
                 cggcggTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTc                                                                                                                                     <  2:1175579/150‑1 (MQ=255)
                  ggtggtGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTGTGGTAACAACGCGACCGCTCATCAGTACTTATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCg                                                                                                                                    <  1:797884/150‑1 (MQ=255)
                  gggggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCg                                                                                                                                    <  1:618987/147‑1 (MQ=255)
                   gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGGTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAACCAGGGCGCAAGCCCTGTTTTTTTTCgg                                                                                                                                   <  2:249228/150‑1 (MQ=255)
                   gtggtgGCAAAGGTGCTGCAGGTGTCCAGCCTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTTGTAACAACGCGACCGCGCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCgg                                                                                                                                   <  2:49675/150‑1 (MQ=255)
                    tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACTACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                  <  2:1548391/150‑1 (MQ=255)
                    tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                  <  2:1488725/150‑1 (MQ=255)
                    tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCggg                                                                                                                                  <  2:1189460/150‑1 (MQ=255)
                      gtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGgag                                                                                                                                <  2:405638/150‑1 (MQ=255)
                      gtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGgag                                                                                                                                >  1:874788/1‑150 (MQ=255)
                          cAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGagaaga                                                                                                                            <  2:1220223/150‑1 (MQ=255)
                                  ctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAAt                                                                                                                    >  2:709457/1‑150 (MQ=255)
                                  ctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAAt                                                                                                                    >  1:1395053/1‑150 (MQ=255)
                                  ctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAAt                                                                                                                    >  2:892825/1‑150 (MQ=255)
                                   tgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGGGACTCAGGTTGGCTTTGGTAACAACGCGCCCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGAGCTAGCCCTGTTTTTTTTCGGGAGATGAATATGACTa                                                                                                                   >  2:1684255/1‑150 (MQ=255)
                                    gcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATAc                                                                                                                  >  1:1649863/1‑150 (MQ=255)
                                     cAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACg                                                                                                                 >  1:1403000/1‑150 (MQ=255)
                                      aGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGt                                                                                                                >  1:409999/1‑150 (MQ=255)
                                      aGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGt                                                                                                                <  1:1451279/150‑1 (MQ=255)
                                       gTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGtt                                                                                                               >  2:1785677/1‑150 (MQ=255)
                                           aCCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGttatta                                                                                                           >  2:1078441/1‑150 (MQ=255)
                                             cAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGACCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACt                                                                                                         >  2:1334893/1‑150 (MQ=255)
                                              aGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTc                                                                                                        <  1:1222408/150‑1 (MQ=255)
                                               gACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTATTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTcc                                                                                                       <  1:717490/150‑1 (MQ=255)
                                                    cATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCg                                                                                                  >  2:1359183/1‑150 (MQ=255)
                                                    cATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCg                                                                                                  <  2:321648/150‑1 (MQ=255)
                                                     aTCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg                                                                                                 <  1:1230007/150‑1 (MQ=255)
                                                     aTCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg                                                                                                 <  2:1550737/150‑1 (MQ=255)
                                                      tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                                <  2:775805/150‑1 (MQ=255)
                                                      tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                                <  2:1477277/150‑1 (MQ=255)
                                                      tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                                <  2:1763041/150‑1 (MQ=255)
                                                      tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                                >  2:520559/1‑150 (MQ=255)
                                                        tAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCAc                                                                                              >  1:1584981/1‑150 (MQ=255)
                                                        tAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCAc                                                                                              >  1:1468621/1‑150 (MQ=255)
                                                             cctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTcc                                                                                         <  1:1160780/150‑1 (MQ=255)
                                                               tccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                       >  2:649871/1‑150 (MQ=255)
                                                               cccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTAATAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                       <  2:562423/149‑1 (MQ=255)
                                                                ccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGAGCAAGCGCTGTTTTTTTTCTGGACAAGAATATGAATACGTAATTACTCCTTGCGGCACTTTCCAGt                                                                                      >  2:339189/1‑150 (MQ=255)
                                                                  gTCCACGTGACTCAGGGTGGCTTTGGTAACAACGCGACCGCTCATCATGACTAATACATCATTTTTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCa                                                                                    <  2:142059/150‑1 (MQ=255)
                                                                   tccaCGGGACTCAGGTTGGCTTTGGTAACAACGCTACCGCTCATCAGTAATAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAg                                                                                   <  2:1113439/147‑1 (MQ=255)
                                                                   tCAAAGTGACTCAGGTTGGATTTGGTAACAACGCGTCCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAg                                                                                   <  2:938391/150‑1 (MQ=255)
                                                                          gACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCt                                                                            <  2:511758/150‑1 (MQ=255)
                                                                           aCTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCtt                                                                           >  1:1488949/1‑150 (MQ=255)
                                                                               aGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAAt                                                                       >  2:254975/1‑150 (MQ=255)
                                                                                ggTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGGTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATa                                                                      >  2:1168044/1‑150 (MQ=255)
                                                                                 gTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATAc                                                                     <  1:555601/150‑1 (MQ=255)
                                                                                      cTTTGGTAACAACGCGACCGCTCATCAGTAGTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGAccc                                                                >  1:720948/1‑150 (MQ=255)
                                                                                      cTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGAccc                                                                >  1:1030762/1‑150 (MQ=255)
                                                                                      cTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGAccc                                                                >  1:1708468/1‑150 (MQ=255)
                                                                                      cTTTGGTAACAACGCGAACGCTCATCAGTACTAATACATCATTAGGATTACAGAAACAGGGCGCAAGCCCTGGTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGAGTCACTTTCCCTTCAGATAACCTTTAATAAGAccc                                                                >  1:290951/1‑150 (MQ=255)
                                                                                        ttGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAg                                                              <  2:1754682/150‑1 (MQ=255)
                                                                                           gTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCa                                                            >  1:1356115/1‑150 (MQ=255)
                                                                                               caacGCTACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACCGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAcggg                                                       >  2:695787/1‑150 (MQ=255)
                                                                                                  cgcgACCGCTCATCATTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtg                                                    <  2:1013127/150‑1 (MQ=255)
                                                                                                  cgcgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtg                                                    <  2:1020594/150‑1 (MQ=255)
                                                                                                    cgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtgtg                                                  <  1:1785677/150‑1 (MQ=255)
                                                                                                    cgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtgtg                                                  <  1:1073670/150‑1 (MQ=255)
                                                                                                       ccGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACGTTCCAGTCAGATAACCTTTACTACGACCCAGCAAGGGGATGTGtat                                               >  2:278347/1‑150 (MQ=255)
                                                                                                         gCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAc                                             >  2:477282/1‑150 (MQ=255)
                                                                                                          ggaatcaCTACGAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAcc                                            <  2:1044883/147‑1 (MQ=255)
                                                                                                            catcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCATAAAACATTTAATACGACCCAGCAAGGGGATGTGTATACCat                                          >  1:107939/1‑150 (MQ=255)
                                                                                                                     tAATACATCATTTGTATTACAGAAACAGTGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGa                                 <  1:1143400/150‑1 (MQ=255)
                                                                                                                       aTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAg                               <  1:1298061/150‑1 (MQ=255)
                                                                                                                          catcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGCCCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCa                            >  2:1286438/1‑150 (MQ=255)
                                                                                                                          catcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCa                            >  1:486427/1‑150 (MQ=255)
                                                                                                                          catcatTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCa                            >  2:155321/1‑150 (MQ=255)
                                                                                                                               tttGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCtt                       <  2:967106/150‑1 (MQ=255)
                                                                                                                               tttGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCtt                       >  1:118879/1‑150 (MQ=255)
                                                                                                                                 tGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGAACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTAc                     >  1:292682/1‑150 (MQ=255)
                                                                                                                                     gaaaaaaaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTTTTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCaa                 >  1:746620/7‑150 (MQ=255)
                                                                                                                                       aCAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGTATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATc               <  2:720948/150‑1 (MQ=255)
                                                                                                                                       aCAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATc               >  1:176989/1‑150 (MQ=255)
                                                                                                                                       aCAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTGGCTGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGCTGTGGATACCATTATTCCTGAAGTCACTCTTACTCAAta               >  2:1116184/1‑149 (MQ=255)
                                                                                                                                        cAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCt              >  1:1623233/1‑150 (MQ=255)
                                                                                                                                         aaaaaCAGGGCTTGCGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGAGTATACCATTATTCCTGAAGTCACTCTTACTCAATCtt             >  1:9932/3‑150 (MQ=255)
                                                                                                                                         aGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCtt             >  2:954590/1‑150 (MQ=255)
                                                                                                                                          gAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTg            <  2:1433315/150‑1 (MQ=255)
                                                                                                                                            aaCAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTc          <  1:1359183/150‑1 (MQ=255)
                                                                                                                                            aaCAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTc          >  2:123260/1‑150 (MQ=255)
                                                                                                                                            aaCAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTc          >  1:800111/1‑150 (MQ=255)
                                                                                                                                             aCAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCt         <  2:874788/150‑1 (MQ=255)
                                                                                                                                                gggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgtg      <  2:546368/150‑1 (MQ=255)
                                                                                                                                                 ggCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGc     >  1:928571/1‑150 (MQ=255)
                                                                                                                                                  gcgcAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCCGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCa    <  2:1403000/150‑1 (MQ=255)
                                                                                                                                                   cgcAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCag   >  1:636377/1‑150 (MQ=255)
                                                                                                                                                    gcAAGCCCTGTTTTTTTTCGCGCGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCACGTGGTTGTGTATACCATTATTCCTGAAGTCACTCTTACTCAAACTTGTCTGTGCaga  <  2:90004/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ATGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGA  >  NC_000913/1104783‑1105080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: