Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,131,199 G→C 6.4% S61T (AGT→ACT) ‡ flgB → flagellar component of cell‑proximal portion of basal‑body rod

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,131,1990GC 6.4% 453.9 / 16.7 139S61T (AGT→ACT) ‡flgBflagellar component of cell‑proximal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base G (64/66);  new base C (7/2);  total (71/68)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

Only 100 of 147 total aligned reads displayed.

TCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCG  >  NC_000913/1131050‑1131348
                                                                                                                                                     |                                                                                                                                                     
tCAACAATAGGCGCTCAATCTGCGCGCCCAGCTTCAGGAAGTGCTGGCATAAAACATCGCCAATGCCGATACCCCTGGTTATCATGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGAAACGTGGACTGGATGCAACCAg                                                                                                                                                       <  2:1480912/150‑1 (MQ=255)
tCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAATAAGTCATGCAACGTGGACGGGATGCAACCAg                                                                                                                                                       <  1:266736/150‑1 (MQ=255)
 caacaaGAGGCGCTCAATCTGCGCGCCCAGGGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAgt                                                                                                                                                      >  1:720571/1‑150 (MQ=255)
 caacaaGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATAGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAgt                                                                                                                                                      >  2:253690/1‑150 (MQ=255)
  aacaaGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTGTCAGGCGCGCGATATCGATTTTGCCAGCGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAgtg                                                                                                                                                     >  2:545589/1‑150 (MQ=255)
        agGCGCGCAATCTGCGCGCCCAGCGTCAGGAAGTGCGGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTg                                                                                                                                               <  2:1635447/150‑1 (MQ=255)
         ggCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGc                                                                                                                                              >  1:1293240/1‑150 (MQ=255)
         ggCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGc                                                                                                                                              >  2:751341/1‑150 (MQ=255)
         ggCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGc                                                                                                                                              >  2:868602/1‑150 (MQ=255)
          gcgcTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACTGGATGCAACCAGTGTGGTTGCa                                                                                                                                             >  1:1321870/1‑150 (MQ=255)
          gcgcTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCa                                                                                                                                             <  1:737320/150‑1 (MQ=255)
             cTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTg                                                                                                                                          <  2:29758/150‑1 (MQ=255)
             cTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTg                                                                                                                                          >  1:1085240/1‑150 (MQ=255)
             cTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCACACTGGTTGCatcc                                                                                                                                          <  1:1023271/150‑4 (MQ=255)
               cAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGAc                                                                                                                                        <  2:1447026/150‑1 (MQ=255)
               cAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGAc                                                                                                                                        >  2:361670/1‑150 (MQ=255)
               cAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGAc                                                                                                                                        <  2:1354415/150‑1 (MQ=255)
               cAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGAc                                                                                                                                        >  1:208706/1‑150 (MQ=255)
               cAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGAc                                                                                                                                        >  1:145254/1‑150 (MQ=255)
                aagcTGCGCGCCCAGCGGCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCCGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGAGTGGTTGCACTGACg                                                                                                                                       <  2:1006233/147‑1 (MQ=255)
                  tCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGAt                                                                                                                                     <  2:1248013/150‑1 (MQ=255)
                   cTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATg                                                                                                                                    >  1:16800/1‑150 (MQ=255)
                      cgcgcCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGAcc                                                                                                                                 >  2:511587/1‑150 (MQ=255)
                       gcgcCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGAGTCGTTGCACTGACGATGACCt                                                                                                                                >  1:577751/1‑150 (MQ=255)
                         gcacAGCGTCAGGAACTGCTGGCAGCAAACATCGCCAATGCCGCTACCGCTGCGTATCAGGCGCGCGATATCGATTTTGCCAGTGCACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCa                                                                                                                              <  2:111516/147‑1 (MQ=255)
                         gcCCAGTGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGCCGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCa                                                                                                                              >  1:1306620/1‑150 (MQ=255)
                           ccAGCGGCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAAc                                                                                                                            <  2:941251/150‑1 (MQ=255)
                                gTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAac                                                                                                                       <  1:343970/150‑1 (MQ=255)
                                  cAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAacac                                                                                                                     <  1:795907/150‑1 (MQ=255)
                                    ggAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACAt                                                                                                                   >  1:1416193/1‑150 (MQ=255)
                                      aaGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTc                                                                                                                 >  1:637581/1‑150 (MQ=255)
                                         tGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCgg                                                                                                              <  1:1674454/150‑1 (MQ=255)
                                            tGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGgcgc                                                                                                           <  2:565027/150‑1 (MQ=255)
                                               cagcaAACATCGGCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAgg                                                                                                        <  1:1343676/150‑1 (MQ=255)
                                               cagcaAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAGCGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCCACACATTCCGGCGCAgg                                                                                                        >  2:766592/1‑150 (MQ=255)
                                                    aaCATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCATCCAGTGTGGTTGCACTGACGATGACCTCAGCGCAACGCATTACGGCGCAGGCGCTg                                                                                                   >  2:1187652/1‑150 (MQ=255)
                                                    aaCATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGGGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTg                                                                                                   <  1:1343162/150‑1 (MQ=255)
                                                         cGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGcc                                                                                              <  2:590490/150‑1 (MQ=255)
                                                         cGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGcc                                                                                              >  2:815989/1‑150 (MQ=255)
                                                           ccAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGcctc                                                                                            <  2:1665981/150‑1 (MQ=255)
                                                                     tACCACTGGTTATCAGGCGAGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATTACCTCAACGAAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGa                                                                                  <  2:668448/150‑1 (MQ=255)
                                                                        cccTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACt                                                                               >  1:1748072/1‑150 (MQ=255)
                                                                              ttATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAACGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCACCGCACCACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCCATa                                                                         >  1:1812727/1‑150 (MQ=255)
                                                                                  cAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGt                                                                     <  1:21872/150‑1 (MQ=255)
                                                                                         gcgATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTTCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCgg                                                              <  2:562021/150‑1 (MQ=255)
                                                                                             tatCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCa                                                          >  2:870751/1‑150 (MQ=255)
                                                                                              atCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAg                                                         >  1:393975/1‑150 (MQ=255)
                                                                                                cGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGcc                                                       <  2:840076/150‑1 (MQ=255)
                                                                                                cGATTATGCAAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGCCGATGACCTCAACGCCACAAATTCCGGCGAAGGCGCTGACGCCTCCTACCGCAGAACTTCAATACCGTATTCCGGACCAGca                                                       >  2:1118093/1‑149 (MQ=255)
                                                                                                   ttttGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTc                                                    <  1:1421787/150‑1 (MQ=255)
                                                                                                     ttGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGc                                                  <  2:1712763/150‑1 (MQ=255)
                                                                                                       gCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCtt                                                >  2:13256/1‑150 (MQ=255)
                                                                                                         cAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGa                                              <  1:361670/150‑1 (MQ=255)
                                                                                                          aGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGAc                                             <  2:1718271/150‑1 (MQ=255)
                                                                                                          aGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGAc                                             <  2:483554/150‑1 (MQ=255)
                                                                                                          aGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGAc                                             <  2:1283366/150‑1 (MQ=255)
                                                                                                          aGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGAc                                             <  2:670170/150‑1 (MQ=255)
                                                                                                           gTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACg                                            >  1:1033217/1‑150 (MQ=255)
                                                                                                            tGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACgg                                           <  2:1085240/150‑1 (MQ=255)
                                                                                                             gAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGt                                          >  2:302704/1‑150 (MQ=255)
                                                                                                              aaCTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTa                                         <  1:868602/150‑1 (MQ=255)
                                                                                                               aCTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGAGCAGGCGATGACGCCTCCTACAGCAGAACTGCAATACCGTAATCCGGACCAGCCTTCGCTTGACGGTaa                                        >  1:1414297/1‑150 (MQ=255)
                                                                                                                cTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAAt                                       <  1:1030825/150‑1 (MQ=255)
                                                                                                                 ttAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATa                                      <  1:1602959/150‑1 (MQ=255)
                                                                                                                  tAAAGAGGTCGTGCGACGGGGGCGGGATGCGACCAGTGTGGTGGCACTGCCGGTGGGCGCGGCGCAGCACATTCCGGCGCAGGCGCTGACGCCGCCGCCCGGTGGGCTGCAAGGCGGGAGTCCGGGCCAGCCTTCGCTTGACGGTAATAc                                     >  2:1184439/1‑150 (MQ=255)
                                                                                                                  tAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATAc                                     >  2:140216/1‑150 (MQ=255)
                                                                                                                  tAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATAc                                     >  1:116278/1‑150 (MQ=255)
                                                                                                                    aaaaaGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCACCGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCg                                   >  1:300598/1‑150 (MQ=255)
                                                                                                                       aaGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATCCCGTCg                                >  1:1226559/1‑150 (MQ=255)
                                                                                                                       aaGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACAATCCGGCGCAGGCGCGGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCg                                <  2:1110261/150‑1 (MQ=255)
                                                                                                                        aGTCATGCAACGTGGACGGGATGCAACCAGTGTGGGTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGa                               <  1:1295336/150‑1 (MQ=255)
                                                                                                                         gTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGat                              >  1:1578243/1‑150 (MQ=255)
                                                                                                                          tCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTATTACCGTcaagc                             <  1:1079860/150‑5 (MQ=255)
                                                                                                                          tCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTATTACCGTcaagc                             <  1:979508/150‑5 (MQ=255)
                                                                                                                             tGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATgg                          <  2:787651/150‑1 (MQ=255)
                                                                                                                             tGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATgg                          <  2:794448/150‑1 (MQ=255)
                                                                                                                              gCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGa                         <  2:415187/150‑1 (MQ=255)
                                                                                                                              gCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATACCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGa                         <  1:815989/150‑1 (MQ=255)
                                                                                                                               cAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGAt                        >  2:1766117/1‑150 (MQ=255)
                                                                                                                                aaCGTGGTCGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCTACGCAACACTTTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATc                       <  1:389611/150‑1 (MQ=255)
                                                                                                                                aaCGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATc                       <  2:1713997/150‑1 (MQ=255)
                                                                                                                                   gTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATcgcg                    <  2:1416193/150‑1 (MQ=255)
                                                                                                                                    tGGACGGGATGCAACCTGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGAAGAACTGCAATACCGTATTCCTGACCAGCCTTCGCTTGACGGTAATACCGTCGATATTGATCGCGa                   <  1:439188/150‑1 (MQ=255)
                                                                                                                                     ggACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAAGACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGaa                  >  2:1134094/1‑150 (MQ=255)
                                                                                                                                       aCGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACg                >  2:910514/1‑150 (MQ=255)
                                                                                                                                         tcagtGCAACCACACTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGcc              >  2:167563/5‑149 (MQ=255)
                                                                                                                                         gggATGCAACCAGGGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCa              <  1:882012/150‑1 (MQ=255)
                                                                                                                                          cagtGCAACCACACTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCAc             >  1:1188172/4‑150 (MQ=255)
                                                                                                                                          cagtGCAACCACACTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCAc             >  2:17004/4‑150 (MQ=255)
                                                                                                                                           gATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCAcc            <  2:1592501/150‑1 (MQ=255)
                                                                                                                                           agtGCAACCACACTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCAcc            >  1:606370/3‑150 (MQ=255)
                                                                                                                                            gtGCAACCACACTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCAccc           >  2:596657/2‑150 (MQ=255)
                                                                                                                                            aTGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCAccc           <  1:511587/150‑1 (MQ=255)
                                                                                                                                             tGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACAGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGACGATATGGATCGCGAACGCACCCa          >  1:1231581/1‑150 (MQ=255)
                                                                                                                                               cAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATTGCGACCGCACCCAGt        >  1:145892/1‑150 (MQ=255)
                                                                                                                                                aaCCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGtt       >  2:1547436/1‑150 (MQ=255)
                                                                                                                                                 aCCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGATCTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGttt      >  1:262545/1‑150 (MQ=255)
                                                                                                                                                  ccAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTg     >  1:465185/1‑150 (MQ=255)
                                                                                                                                                    aGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGcc   <  2:1748072/150‑1 (MQ=255)
                                                                                                                                                     gtgtGGTTGCACTGACGATGACCTCAACGCACCACCTTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCg  <  2:261945/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCG  >  NC_000913/1131050‑1131348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: