Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,181,760 A→G 6.0% intergenic (‑35/+23) ymfA ← / ← potD DUF3592 family inner membrane protein/spermidine/putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,181,7600AG 6.0% 354.5 / 8.7 116intergenic (‑35/+23)ymfA/potDDUF3592 family inner membrane protein/spermidine/putrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (66/43);  new base G (2/5);  total (68/48)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

GAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACT  >  NC_000913/1181611‑1181909
                                                                                                                                                     |                                                                                                                                                     
gAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGATAACTAAAAAACGGGCGGTa                                                                                                                                                       >  1:255118/1‑150 (MQ=255)
 aaTCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCAACATCCTTGCTAACTAAAAAACGGGCGGTaa                                                                                                                                                      >  2:1025930/1‑150 (MQ=255)
     gcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATACTTGCTAACTAAAAAACGGGCGGTAATacc                                                                                                                                                  >  1:1610099/1‑150 (MQ=255)
          tttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgc                                                                                                                                             >  1:40623/1‑150 (MQ=255)
           ttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTAccgcc                                                                                                                                            <  1:45681/150‑1 (MQ=255)
              cTATAGGTTAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTAccgcccgt                                                                                                                                         <  1:369542/150‑2 (MQ=255)
              cTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTCCTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTAccgcccgt                                                                                                                                         <  1:716318/150‑2 (MQ=255)
              cTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGCAATATACTAAAGAAAAATTTACTGTCCTGACTCATTAGCCAAATCATTGCTAACTAAAAAACGGGCGGTAATACCAccgcccgc                                                                                                                                         >  1:37784/1‑150 (MQ=255)
               tataGGTGAACTGGTTAAAAAAGACAACAACATAAATGAGGATCAGTACAAGACCTATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTAATTAGACACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTAccgcccgtt                                                                                                                                        <  2:1610026/150‑3 (MQ=255)
                ataGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTACCGCCCGttt                                                                                                                                       <  2:1220385/150‑1 (MQ=255)
                ataGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCtt                                                                                                                                       <  1:269500/150‑1 (MQ=255)
                   ggTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCt                                                                                                                                    <  1:1013005/150‑1 (MQ=255)
                     tGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGa                                                                                                                                  <  1:1261247/150‑1 (MQ=255)
                       aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCACTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAAt                                                                                                                                <  2:879089/150‑1 (MQ=255)
                        aCTAGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAAtt                                                                                                                               >  2:1676377/1‑150 (MQ=255)
                           ggTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAAc                                                                                                                            >  1:1328375/1‑150 (MQ=255)
                           ggTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAAc                                                                                                                            >  1:1796889/1‑150 (MQ=255)
                           ggTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAAc                                                                                                                            >  1:1614296/1‑150 (MQ=255)
                           ggTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAAc                                                                                                                            >  1:1607163/1‑150 (MQ=255)
                                    aGACAACAACACAAATGAGGATAAGTACCAGAACGATACCTAGGAATATACGAAAGAAAACTTTACTGTCATGACTCATTAGCCACATCCATGCTAACTAAAAAACGGGCGGTAATACAACCGCCCGCTTGATGAATTAACGACCTGCtt                                                                                                                   >  2:1286212/1‑150 (MQ=255)
                                       caacaacaGAAATGAGGATCAGTACGAGACCGATACCGAGGAATATACGTAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCATGCTAACTAAAAATCGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCa                                                                                                                <  2:185493/150‑1 (MQ=255)
                                         acaacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGc                                                                                                              <  1:1018746/150‑1 (MQ=255)
                                         acaacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACAACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGc                                                                                                              >  1:500765/1‑150 (MQ=255)
                                          caacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCt                                                                                                             >  2:604731/1‑150 (MQ=255)
                                          caacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCt                                                                                                             >  2:392707/1‑150 (MQ=255)
                                            acaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTc                                                                                                           >  1:275649/1‑150 (MQ=255)
                                             caGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCt                                                                                                          >  2:1152264/1‑150 (MQ=255)
                                                 aaTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGata                                                                                                      <  2:406332/150‑1 (MQ=255)
                                                 aaTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACAGCACGCTTGCTGAATTAAGGACCTGCTTTAAGCTTCTGata                                                                                                      >  1:119221/1‑150 (MQ=255)
                                                  aTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGataa                                                                                                     >  1:103727/1‑150 (MQ=255)
                                                  aTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGataa                                                                                                     >  2:1158370/1‑150 (MQ=255)
                                                    gAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGataata                                                                                                   >  1:1409446/1‑150 (MQ=255)
                                                      ggATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATAct                                                                                                 >  2:1787925/1‑150 (MQ=255)
                                                       gATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATActc                                                                                                >  2:1092696/1‑150 (MQ=255)
                                                       gATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATActc                                                                                                <  2:1807782/150‑1 (MQ=255)
                                                        aTCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATActct                                                                                               >  2:851568/1‑150 (MQ=255)
                                                         tCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCtt                                                                                              >  2:948829/1‑150 (MQ=255)
                                                           aGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCa                                                                                            <  1:1387356/150‑1 (MQ=255)
                                                            ttACCAGACCGATACCGAGGAATATAAGAAAGAAAACTTTACTGTACTGACTCATTAGCCACATCCTTGCTAACTAAAAAACTGGCGGTAATACCACCGCCCGCTTGCTTAATTAAATTCATGCTTTCAGCTTCTGATAATACTCTTCAt                                                                                           <  2:623435/149‑1 (MQ=255)
                                                                cAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAt                                                                                       <  2:912160/150‑1 (MQ=255)
                                                                cAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAt                                                                                       >  2:1366635/1‑150 (MQ=255)
                                                                 aGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtg                                                                                      >  1:814153/1‑150 (MQ=255)
                                                                 aGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtg                                                                                      <  1:189420/150‑1 (MQ=255)
                                                                  gACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtgc                                                                                     >  2:397269/1‑150 (MQ=255)
                                                                    ccGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtgctg                                                                                   >  2:514507/1‑150 (MQ=255)
                                                                          ccGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTgctggct                                                                             >  2:957260/1‑150 (MQ=255)
                                                                          ccGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTgctggct                                                                             >  2:475313/1‑150 (MQ=255)
                                                                               gAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGcc                                                                        <  1:109174/150‑1 (MQ=255)
                                                                               gAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGcc                                                                        <  1:190179/150‑1 (MQ=255)
                                                                                   ataCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACg                                                                    <  1:137828/150‑1 (MQ=255)
                                                                                    taCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGt                                                                   >  2:1789280/1‑150 (MQ=255)
                                                                                    taCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGt                                                                   >  2:1782177/1‑150 (MQ=255)
                                                                                      cGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCa                                                                 >  1:1494979/1‑150 (MQ=255)
                                                                                      cGAAAGAAAACTTTACTGTCCTCACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACAACAGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCAGCGCCAACGtaa                                                                 >  2:1179361/1‑148 (MQ=255)
                                                                                         aagaaaACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTc                                                              <  1:1148631/150‑1 (MQ=255)
                                                                                            aaaaCTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGc                                                           <  2:354361/150‑1 (MQ=255)
                                                                                              aaCTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCAAACGTCATTCTGCCa                                                         <  2:114165/150‑1 (MQ=255)
                                                                                               aCTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCAt                                                        >  1:639289/1‑150 (MQ=255)
                                                                                                   tACTGTCCTGACTCATTAGCCACATCCTTGCTAACTATAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGc                                                    <  1:1154203/150‑1 (MQ=255)
                                                                                                   tACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGc                                                    <  1:342700/150‑1 (MQ=255)
                                                                                                      tGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCAt                                                 >  1:149471/1‑150 (MQ=255)
                                                                                                         ccTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGGCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCAtttt                                              <  1:272474/150‑1 (MQ=255)
                                                                                                           tGACTCAATAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTa                                            <  1:1662615/150‑1 (MQ=255)
                                                                                                             aCTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAAt                                          >  1:336232/1‑150 (MQ=255)
                                                                                                              cTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATg                                         <  2:589868/150‑1 (MQ=255)
                                                                                                                cATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCATTTTTAATGGtt                                      <  1:606978/150‑1 (MQ=255)
                                                                                                                 aTTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGtt                                      >  2:1443167/1‑150 (MQ=255)
                                                                                                                   tAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTc                                    <  2:275649/150‑1 (MQ=255)
                                                                                                                    aGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCCGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCa                                   >  1:120083/1‑150 (MQ=255)
                                                                                                                    aGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCa                                   <  2:498061/150‑1 (MQ=255)
                                                                                                                     gCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGACCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTTCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAg                                  >  1:1092683/1‑150 (MQ=255)
                                                                                                                       cacaTCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCATCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTTATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGAAATTATGAAATTCACCAATTTTAATGGATTCAGCa                                >  1:908295/1‑150 (MQ=255)
                                                                                                                           tCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCg                            >  1:1386253/1‑150 (MQ=255)
                                                                                                                             cTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCggg                          >  2:672399/1‑150 (MQ=255)
                                                                                                                               tGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTa                        <  1:1787925/150‑1 (MQ=255)
                                                                                                                               tGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTa                        >  1:1088815/1‑150 (MQ=255)
                                                                                                                                  taactaaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTagag                     >  1:446858/1‑150 (MQ=255)
                                                                                                                                     ctaaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAgtgt                  >  1:334177/1‑150 (MQ=255)
                                                                                                                                     ctaaAAAACGGGCGGTAATAACACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAgtgt                  >  1:1292739/1‑150 (MQ=255)
                                                                                                                                      taaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGtt                 >  1:1423137/1‑150 (MQ=255)
                                                                                                                                      taaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGtt                 >  1:887053/1‑150 (MQ=255)
                                                                                                                                       aaaaaaCGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGttt                >  1:48509/1‑150 (MQ=255)
                                                                                                                                       aaaaaaCGGGCGGTAATACCACCGCCCGCTTGATGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGTTAGAGTGttt                >  2:1661292/1‑150 (MQ=255)
                                                                                                                                       aaaaaaCGGGCGGTAATAACACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGttt                <  2:37784/150‑1 (MQ=255)
                                                                                                                                        gcaagcGGGCGGTGGTATTACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGtttt               >  1:1312680/6‑150 (MQ=255)
                                                                                                                                          aaaCGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTAt             >  2:526978/1‑150 (MQ=255)
                                                                                                                                          aaaCGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTAt             <  1:841769/150‑1 (MQ=255)
                                                                                                                                          aaaCGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTAt             <  1:269307/150‑1 (MQ=255)
                                                                                                                                           aaCGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCTTAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTGCTCCTTCCGGGTAGAGTGTTTTATc            >  2:517552/1‑150 (MQ=255)
                                                                                                                                            aCGGGCGGTAATACCACCGCCCGCTTTCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCg           <  2:1328375/150‑1 (MQ=255)
                                                                                                                                              gggCGGTAATACCACCACCCGCTTGCTGAAATAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGtt         >  1:1763703/1‑150 (MQ=255)
                                                                                                                                                gCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCg       >  2:699567/1‑150 (MQ=255)
                                                                                                                                                gCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCg       >  2:1564336/1‑150 (MQ=255)
                                                                                                                                                gCGGTAATACAACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATTATACTCTTCATAAATGCTGCTGGCTGGGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCg       >  1:943809/1‑150 (MQ=255)
                                                                                                                                                  ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc     <  1:1676377/150‑1 (MQ=255)
                                                                                                                                                  ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc     <  2:103727/150‑1 (MQ=255)
                                                                                                                                                  ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc     <  1:1152264/150‑1 (MQ=255)
                                                                                                                                                  ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc     <  2:1494979/150‑1 (MQ=255)
                                                                                                                                                   gTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCa    >  1:823368/1‑150 (MQ=255)
                                                                                                                                                   gTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAAGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCa    >  2:1678383/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACT  >  NC_000913/1181611‑1181909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: