Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,181,761 A→G 6.0% intergenic (‑36/+22) ymfA ← / ← potD DUF3592 family inner membrane protein/spermidine/putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,181,7610AG 6.0% 349.6 / 9.8 116intergenic (‑36/+22)ymfA/potDDUF3592 family inner membrane protein/spermidine/putrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (66/43);  new base G (2/5);  total (68/48)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 118 total aligned reads displayed.

AATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACT  >  NC_000913/1181612‑1181909
                                                                                                                                                     |                                                                                                                                                    
    gcgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATACTTGCTAACTAAAAAACGGGCGGTAATacc                                                                                                                                                  >  1:1610099/1‑150 (MQ=255)
      gTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATaccac                                                                                                                                                <  1:1513133/150‑1 (MQ=255)
         tttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgc                                                                                                                                             >  1:40623/1‑150 (MQ=255)
          ttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTAccgcc                                                                                                                                            <  1:45681/150‑1 (MQ=255)
            gCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgcccc                                                                                                                                          >  2:1510200/1‑149 (MQ=255)
             cTATAGGTTAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTAccgcccgt                                                                                                                                         <  1:369542/150‑2 (MQ=255)
             cTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTCCTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTAccgcccgt                                                                                                                                         <  1:716318/150‑2 (MQ=255)
             cTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGCAATATACTAAAGAAAAATTTACTGTCCTGACTCATTAGCCAAATCATTGCTAACTAAAAAACGGGCGGTAATACCAccgcccgc                                                                                                                                         >  1:37784/1‑150 (MQ=255)
              tataGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCt                                                                                                                                        >  2:189420/1‑150 (MQ=255)
              tataGGTGAACTGGTTAAAAAAGACAACAACATAAATGAGGATCAGTACAAGACCTATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTAATTAGACACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTAccgcccgtt                                                                                                                                        <  2:1610026/150‑3 (MQ=255)
               ataGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTACCGCCCGttt                                                                                                                                       <  2:1220385/150‑1 (MQ=255)
                  ggTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCt                                                                                                                                    <  2:1091262/150‑1 (MQ=255)
                  ggTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCt                                                                                                                                    <  1:1013005/150‑1 (MQ=255)
                      aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCACTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAAt                                                                                                                                <  2:879089/150‑1 (MQ=255)
                       aCTAGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAAtt                                                                                                                               >  2:1676377/1‑150 (MQ=255)
                          ggTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAAc                                                                                                                            >  1:1328375/1‑150 (MQ=255)
                          ggTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAAc                                                                                                                            >  1:1796889/1‑150 (MQ=255)
                          ggTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAAc                                                                                                                            >  1:1614296/1‑150 (MQ=255)
                                   aGACAACAACACAAATGAGGATAAGTACCAGAACGATACCTAGGAATATACGAAAGAAAACTTTACTGTCATGACTCATTAGCCACATCCATGCTAACTAAAAAACGGGCGGTAATACAACCGCCCGCTTGATGAATTAACGACCTGCtt                                                                                                                   >  2:1286212/1‑150 (MQ=255)
                                      caacaacaGAAATGAGGAGCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGGCCGCTTGCTGAATTAACGTCCTGCTTTCa                                                                                                                <  2:472218/150‑1 (MQ=255)
                                        acaacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGc                                                                                                              <  1:1018746/150‑1 (MQ=255)
                                        acaacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGc                                                                                                              >  1:430479/1‑150 (MQ=255)
                                        acaacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACAACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGc                                                                                                              >  1:500765/1‑150 (MQ=255)
                                         caacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCt                                                                                                             >  2:392707/1‑150 (MQ=255)
                                         caacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCt                                                                                                             >  2:604731/1‑150 (MQ=255)
                                          aacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCtt                                                                                                            >  1:230077/1‑150 (MQ=255)
                                           acaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTc                                                                                                           >  1:275649/1‑150 (MQ=255)
                                            caGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCt                                                                                                          >  2:1152264/1‑150 (MQ=255)
                                                aaTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACAGCACGCTTGCTGAATTAAGGACCTGCTTTAAGCTTCTGata                                                                                                      >  1:119221/1‑150 (MQ=255)
                                                 aTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGataa                                                                                                     >  2:1158370/1‑150 (MQ=255)
                                                 aTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGataa                                                                                                     >  1:103727/1‑150 (MQ=255)
                                                   gAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGataata                                                                                                   >  1:1409446/1‑150 (MQ=255)
                                                     ggATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATAct                                                                                                 >  2:1787925/1‑150 (MQ=255)
                                                      gATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATActc                                                                                                >  2:1092696/1‑150 (MQ=255)
                                                      gATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATActc                                                                                                <  2:1807782/150‑1 (MQ=255)
                                                       aTCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATActct                                                                                               >  2:851568/1‑150 (MQ=255)
                                                        tCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCtt                                                                                              >  2:948829/1‑150 (MQ=255)
                                                          aGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCa                                                                                            <  1:1387356/150‑1 (MQ=255)
                                                           ttACCAGACCGATACCGAGGAATATAAGAAAGAAAACTTTACTGTACTGACTCATTAGCCACATCCTTGCTAACTAAAAAACTGGCGGTAATACCACCGCCCGCTTGCTTAATTAAATTCATGCTTTCAGCTTCTGATAATACTCTTCAt                                                                                           <  2:623435/149‑1 (MQ=255)
                                                               cAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAt                                                                                       >  2:1366635/1‑150 (MQ=255)
                                                               cAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAt                                                                                       <  2:912160/150‑1 (MQ=255)
                                                                aGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtg                                                                                      <  1:189420/150‑1 (MQ=255)
                                                                 gACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtgc                                                                                     >  2:397269/1‑150 (MQ=255)
                                                                   ccGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtgctg                                                                                   >  2:514507/1‑150 (MQ=255)
                                                                    cGATACCGAGGAATATACGAAAGAAAACTTTCCTGTCCTGACTCATTAGCCACCTCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtgctgc                                                                                  >  1:285343/1‑150 (MQ=255)
                                                                         ccGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTgctggct                                                                             >  2:475313/1‑150 (MQ=255)
                                                                         ccGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTgctggct                                                                             >  2:957260/1‑150 (MQ=255)
                                                                              gAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGcc                                                                        <  1:109174/150‑1 (MQ=255)
                                                                              gAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGcc                                                                        <  1:190179/150‑1 (MQ=255)
                                                                                  ataCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACg                                                                    <  1:137828/150‑1 (MQ=255)
                                                                                  ataCGAAAGAAAACTTTACTGTCCTGACTCATTACCCACATCCTTGCTAACTAAAAAACGGGCTGTAATACCACCGCCCGCTTGATGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCGGTCTGCGCCAACg                                                                    <  1:170275/150‑1 (MQ=255)
                                                                                   taCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGt                                                                   >  2:1782177/1‑150 (MQ=255)
                                                                                   taCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGt                                                                   >  2:1789280/1‑150 (MQ=255)
                                                                                   taCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGt                                                                   >  2:1259117/1‑150 (MQ=255)
                                                                                    aCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTc                                                                  <  2:551505/150‑1 (MQ=255)
                                                                                     cGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCa                                                                 >  1:1494979/1‑150 (MQ=255)
                                                                                     cGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGACCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCa                                                                 >  1:1235621/1‑150 (MQ=255)
                                                                                        aagaaaACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTc                                                              <  1:1148631/150‑1 (MQ=255)
                                                                                           aaaaCTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGc                                                           <  2:354361/150‑1 (MQ=255)
                                                                                             aaCTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCAAACGTCATTCTGCCa                                                         <  2:114165/150‑1 (MQ=255)
                                                                                              aCTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCAt                                                        >  1:639289/1‑150 (MQ=255)
                                                                                                  tACTGTCCTGACTCATTAGCCACATCCTTGCTAACTATAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGc                                                    <  1:1154203/150‑1 (MQ=255)
                                                                                                     tGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCAt                                                 >  1:149471/1‑150 (MQ=255)
                                                                                                        ccTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCAtttt                                              >  1:608043/1‑150 (MQ=255)
                                                                                                        ccTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGGCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCAtttt                                              <  1:272474/150‑1 (MQ=255)
                                                                                                          tGACTCAATAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTa                                            <  1:1662615/150‑1 (MQ=255)
                                                                                                            aCTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAAt                                          >  1:336232/1‑150 (MQ=255)
                                                                                                             cTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATg                                         <  2:589868/150‑1 (MQ=255)
                                                                                                               cATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCATTTTTAATGGtt                                      <  1:606978/150‑1 (MQ=255)
                                                                                                                  tAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTc                                    <  2:275649/150‑1 (MQ=255)
                                                                                                                   aGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCCGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCa                                   >  1:120083/1‑150 (MQ=255)
                                                                                                                   aGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCa                                   <  2:498061/150‑1 (MQ=255)
                                                                                                                    gCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGACCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTTCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAg                                  >  1:1092683/1‑150 (MQ=255)
                                                                                                                      cacaTCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCATCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTTATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGAAATTATGAAATTCACCAATTTTAATGGATTCAGCa                                >  1:908295/1‑150 (MQ=255)
                                                                                                                            cTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCggg                          >  2:672399/1‑150 (MQ=255)
                                                                                                                              tGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTa                        >  1:1088815/1‑150 (MQ=255)
                                                                                                                              tGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTa                        <  1:1787925/150‑1 (MQ=255)
                                                                                                                                 taactaaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTagag                     >  1:446858/1‑150 (MQ=255)
                                                                                                                                    ctaaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAgtgt                  >  1:334177/1‑150 (MQ=255)
                                                                                                                                    ctaaAAAACGGGCGGTAATAACACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAgtgt                  >  1:1292739/1‑150 (MQ=255)
                                                                                                                                     taaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGtt                 >  1:1423137/1‑150 (MQ=255)
                                                                                                                                     taaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGtt                 >  1:887053/1‑150 (MQ=255)
                                                                                                                                      aaaaaaCGGGCGGTAATACCACCGCCCGCTTGATGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGTTAGAGTGttt                >  2:1661292/1‑150 (MQ=255)
                                                                                                                                      aaaaaaCGGGCGGTAATAACACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGttt                <  2:37784/150‑1 (MQ=255)
                                                                                                                                       gcaagcGGGCGGTGGTATTACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGtttt               >  1:1312680/6‑150 (MQ=255)
                                                                                                                                         aaaCGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTAt             <  1:841769/150‑1 (MQ=255)
                                                                                                                                         aaaCGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTAt             <  1:269307/150‑1 (MQ=255)
                                                                                                                                          aaCGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCTTAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTGCTCCTTCCGGGTAGAGTGTTTTATc            >  2:517552/1‑150 (MQ=255)
                                                                                                                                           gcGGGCGGTGGTATTACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCg           >  2:1773244/2‑150 (MQ=255)
                                                                                                                                           aCGGGCGGTAATACCACCGCCCGCTTTCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCg           <  2:1328375/150‑1 (MQ=255)
                                                                                                                                               gCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCg       >  2:699567/1‑150 (MQ=255)
                                                                                                                                               gCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCg       >  2:1564336/1‑150 (MQ=255)
                                                                                                                                               gCGGTAATACAACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATTATACTCTTCATAAATGCTGCTGGCTGGGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCg       >  1:943809/1‑150 (MQ=255)
                                                                                                                                                 ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc     <  2:1494979/150‑1 (MQ=255)
                                                                                                                                                 ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc     <  2:103727/150‑1 (MQ=255)
                                                                                                                                                 ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc     <  1:1152264/150‑1 (MQ=255)
                                                                                                                                                 ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc     <  1:851568/150‑1 (MQ=255)
                                                                                                                                                 ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc     <  1:1676377/150‑1 (MQ=255)
                                                                                                                                                  gTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCa    >  1:823368/1‑150 (MQ=255)
                                                                                                                                                    aaTACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACt  <  1:1385840/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACT  >  NC_000913/1181612‑1181909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: