Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 209,171 A→G 5.4% N184S (AAC→AGC) ‡ accA → acetyl‑CoA carboxylase, carboxytransferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913209,1710AG 5.4% 375.5 / 8.7 129N184S (AAC→AGC) ‡accAacetyl‑CoA carboxylase, carboxytransferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (67/53);  new base G (4/3);  total (72/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

Only 100 of 133 total aligned reads displayed.

CAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTG  >  NC_000913/209024‑209320
                                                                                                                                                   |                                                                                                                                                     
cAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGCACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAccc                                                                                                                                                     >  1:227280/1‑150 (MQ=255)
cAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCTGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGACATTGCACGCAAcc                                                                                                                                                     >  2:286377/1‑150 (MQ=255)
 aGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCCCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCt                                                                                                                                                    >  1:82354/1‑150 (MQ=255)
 aGCGCCAGAAGGTTACCGAAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGAGGTCAGTCTGAAGCCATTGCACGCAACCt                                                                                                                                                    >  1:1196014/1‑150 (MQ=255)
  gcgcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTg                                                                                                                                                   >  1:965456/1‑150 (MQ=255)
  gcgcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTATAAGATGCCTATCATCCCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTg                                                                                                                                                   >  2:1088898/1‑150 (MQ=255)
    gcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCCCCTTTATCGACACCCCGGGGGCATATCCTGGAGTTGGCGCAGAAGCGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCg                                                                                                                                                 >  2:1007206/1‑150 (MQ=255)
        gAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACCCCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGaa                                                                                                                                             >  1:1288027/1‑150 (MQ=255)
        gAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCCGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGaa                                                                                                                                             <  2:1465973/150‑1 (MQ=255)
         aaGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGaaa                                                                                                                                            <  2:1350031/150‑1 (MQ=255)
           ggTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAGGTTGCGTGCAATg                                                                                                                                          <  1:818674/150‑1 (MQ=255)
            gTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTTCACGCAGGTTGCGTGCAATgg                                                                                                                                         <  1:1254893/150‑2 (MQ=255)
            gTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTTCACGCAGGTTGCGTGCAATgg                                                                                                                                         <  1:631809/150‑2 (MQ=255)
                ccGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCg                                                                                                                                     >  1:805266/1‑150 (MQ=255)
                 cGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTAACGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGc                                                                                                                                    >  2:826921/1‑150 (MQ=255)
                    aaaGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTc                                                                                                                                 <  2:677056/150‑1 (MQ=255)
                       gCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATCCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGGCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGc                                                                                                                              <  2:1006213/150‑1 (MQ=255)
                            gCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTAcc                                                                                                                         >  1:1278710/1‑150 (MQ=255)
                              gTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCgg                                                                                                                       <  1:770318/150‑1 (MQ=255)
                               tCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCACCCTGCGTGAAATGTCACGCCTCGGCGTCCCGGt                                                                                                                      >  1:1635119/1‑150 (MQ=255)
                                cTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTa                                                                                                                     >  2:1479993/1‑150 (MQ=255)
                                cTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCCACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTa                                                                                                                     >  2:955803/1‑150 (MQ=255)
                                  gATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGt                                                                                                                   >  2:1478684/1‑150 (MQ=255)
                                   aTGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGtt                                                                                                                  <  1:310658/150‑1 (MQ=255)
                                    tGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGttt                                                                                                                 >  2:1665533/1‑150 (MQ=255)
                                     gCAAATGGCTGAAGGCTTTAAGATGCCTATCATCCCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTg                                                                                                                <  2:1431594/150‑1 (MQ=255)
                                     gCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTg                                                                                                                <  2:1345569/150‑1 (MQ=255)
                                      cAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGt                                                                                                               >  1:1608300/1‑150 (MQ=255)
                                      cAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGt                                                                                                               >  1:1019936/1‑150 (MQ=255)
                                      cAAATGGCTGAACGCTTTAAGATGCCAATCATCACATTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGAGGTCTGTCTGAAGCCATTGCACGCAACCTGCGAGAAATGTCTCGCCTCGGCGTACCGGGAGTTAGt                                                                                                               >  1:871580/1‑150 (MQ=255)
                                           ggCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCACTGGGGATTATCCTGGCGTGGGAGAAGAAGAGCTTGGTCAGTCTGAAGACATTGCACGCAACCTGCGTGAAATGTCTAGCCTCGGCGTACCGGTAGTTTGTACGGt                                                                                                          >  1:1280066/1‑150 (MQ=255)
                                               gAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCC‑GGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCg                                                                                                     >  1:1773431/1‑150 (MQ=255)
                                                aaCGCTTTAAGATGCCTATCATCACCTTTATCGACAACCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCTTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCg                                                                                                     <  2:1734234/150‑1 (MQ=255)
                                                      ttAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAg                                                                                               <  2:1800742/150‑1 (MQ=255)
                                                       tAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTAACCTAGCGTGGGCGCAGAAGAGAGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCCTGAAATGTCTCGCCTAGGCGAACCTGTAGTTTGTACGGTTATCGGTGAAgg                                                                                              >  1:883013/1‑150 (MQ=255)
                                                             gCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTc                                                                                        >  2:1362992/1‑150 (MQ=255)
                                                               cTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTg                                                                                      >  2:171920/1‑150 (MQ=255)
                                                                 atcatcACCTTTATCGCGACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGc                                                                                    <  1:145918/150‑1 (MQ=255)
                                                                  tcatcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGTGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCg                                                                                   <  1:1592955/150‑1 (MQ=255)
                                                                  tcatcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCg                                                                                   <  1:251212/150‑1 (MQ=255)
                                                                   catcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCgg                                                                                  <  1:1774277/150‑1 (MQ=255)
                                                                     tcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTg                                                                                <  1:664128/150‑1 (MQ=255)
                                                                      caCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTTTCGGTGAAGGTGGTTCTGGCGGTgc                                                                               >  1:224819/1‑150 (MQ=255)
                                                                       aCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGGCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcg                                                                              <  2:1447773/150‑1 (MQ=255)
                                                                        ccTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcgc                                                                             >  2:855102/1‑150 (MQ=255)
                                                                        ccTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGCCGTACCGGTAGTTTGTACGGTTATCGGTGATGGTGGTTCTGGCGGTgcgc                                                                             >  2:659904/1‑150 (MQ=255)
                                                                          tttATCGTCACCCCGGGGGCTTATGCTGGCGGGGGCGCAGAAGAGCGGGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGTCGGTGCGCTg                                                                           <  1:505655/150‑1 (MQ=255)
                                                                          tttATCGACACCCCGGGGGCTTATCCTGGCCGGGGCGCAGAAGAGCGTTGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGGGAAGGTGGTTCTGGCGGTGCGCTg                                                                           <  2:142027/150‑1 (MQ=255)
                                                                           ttATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGGACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTgg                                                                          <  2:525834/150‑1 (MQ=255)
                                                                           ttATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTgg                                                                          >  1:221274/1‑150 (MQ=255)
                                                                            aaTCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGGCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGc                                                                         <  1:1502563/149‑1 (MQ=255)
                                                                             aTCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACTAAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTCGTTCTGGCGGTGCGCTGGCg                                                                        <  2:1764973/150‑1 (MQ=255)
                                                                             aTCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGGAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCg                                                                        >  2:1095386/1‑150 (MQ=255)
                                                                               cGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGTAGGTGGTTCTGGCGGTGCGCTGGCGAt                                                                      >  1:127396/1‑150 (MQ=255)
                                                                                gACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAtt                                                                     >  1:885115/1‑150 (MQ=255)
                                                                                gACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAtt                                                                     >  1:378302/1‑150 (MQ=255)
                                                                                  cacCCCGGGTGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                                                   <  2:849020/150‑1 (MQ=255)
                                                                                  cacCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                                                   >  2:22344/1‑150 (MQ=255)
                                                                                  cacCCCGGGGGCGTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                                                   <  1:1102531/150‑1 (MQ=255)
                                                                                   cccccGGGGGCTTATCCTGGCGGGGGCGCAGAAGAGCGGGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGc                                                                  <  1:321424/149‑1 (MQ=255)
                                                                                    ccccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCg                                                                 <  2:798448/150‑1 (MQ=255)
                                                                                    ccccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCg                                                                 <  2:1621444/150‑1 (MQ=255)
                                                                                      ccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGGCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGGCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTg                                                               <  1:1171809/150‑1 (MQ=255)
                                                                                         ggggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGc                                                            <  2:652057/150‑1 (MQ=255)
                                                                                          gggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTTAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCg                                                           <  2:955940/150‑1 (MQ=255)
                                                                                           ggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGa                                                          >  1:1001762/1‑150 (MQ=255)
                                                                                              ttATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATaa                                                       >  2:159343/1‑150 (MQ=255)
                                                                                                 tCCTGGCGTGGGCGCAGAAGAGCGTTGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGt                                                    <  1:1112505/150‑1 (MQ=255)
                                                                                                  ccTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTg                                                   >  2:1720216/1‑150 (MQ=255)
                                                                                                   cTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGa                                                  >  1:1464314/1‑150 (MQ=255)
                                                                                                          gggCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgct                                           <  1:1448549/150‑1 (MQ=255)
                                                                                                           ggCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgctg                                          >  1:519679/1‑150 (MQ=255)
                                                                                                            gcgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCGACCTGCGTGAATTGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGGGATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGGGATTGGCGTGGGCGAGAAAGTGAATAtgctgc                                         >  1:76220/1‑150 (MQ=255)
                                                                                                            gcgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgctgc                                         <  2:745284/150‑1 (MQ=255)
                                                                                                             cgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCa                                        >  2:1201484/1‑150 (MQ=255)
                                                                                                             cgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGAGAAAGTGAATATGCTGCa                                        >  2:1801940/1‑150 (MQ=255)
                                                                                                              gcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCaa                                       <  1:733731/150‑1 (MQ=255)
                                                                                                              gcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCaa                                       >  2:424580/1‑150 (MQ=255)
                                                                                                                  aagaGCTTGCTCAGTCTGAAGCCATTGCATTCAAGATGCGTGAAATGTCTCTCATAGTCGTACCGGTAGTTTGTAAGGTTATCGGTGAAGGTTTTTCTGTCGGTGCTCTTGCGATTGGCGTGGGCGATAAAGTTAATATGCTGCAATACa                                   <  1:286377/150‑1 (MQ=255)
                                                                                                                   agagCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAg                                  <  1:1277390/150‑1 (MQ=255)
                                                                                                                        gTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCt                             >  1:1049009/1‑150 (MQ=255)
                                                                                                                            tCAGTCTGAAGCCATTGCTCTCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGGTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTc                         <  1:442735/150‑1 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGt                      >  1:288030/1‑150 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGt                      <  1:1478684/150‑1 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGt                      >  2:652606/1‑150 (MQ=255)
                                                                                                                                 cTGAAGCCAGTGCACGCAACCTGCGGGAAATGTCCCGACTCCGCGTACCGGTAGTTTTTTCTGTTATCTGTGAAGGTGGTTCTGGCGTTGCGCTGGCGATTGGCGTGGGCGATTAAGTGAATATGCTGGAATACAGCACCTATTCCGTTa                    <  2:273728/150‑1 (MQ=255)
                                                                                                                                  gcgagacATTTCACGCAGGTTGCGTGCAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTTGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAt                   >  2:68024/7‑150 (MQ=255)
                                                                                                                                   gAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAta                  >  2:386198/1‑149 (MQ=255)
                                                                                                                                     aGCCATTGGACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGTAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAtctc                <  1:11960/150‑1 (MQ=255)
                                                                                                                                     aGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTCTTCCGTTAtctc                >  1:1752566/1‑150 (MQ=255)
                                                                                                                                      gacATTTCACGCAGGTTGCGTGCAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCAGTTATCTCg               >  1:1568317/3‑150 (MQ=255)
                                                                                                                                      gCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCg               >  2:673162/1‑150 (MQ=255)
                                                                                                                                        cATTTCACGCAGGTTGCGTGCAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGcc             >  1:695450/1‑150 (MQ=255)
                                                                                                                                        cATTTCACGCAGGTTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACATCACCTATTACGTTATCTCGcc             >  2:125720/1‑150 (MQ=255)
                                                                                                                                        cATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGcc             <  1:1020471/150‑1 (MQ=255)
                                                                                                                                        cATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGcc             <  2:1288027/150‑1 (MQ=255)
                                                                                                                                         aTTGCACGCAACCTGCGTGAAATGTCTCGCCTCTGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGGGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCg            <  2:917588/150‑1 (MQ=255)
                                                                                                                                           tGCACGAAACCTGCGTGAAATGTCTCGCCTCGGCGTACCCGTAGTTTGTACGGTTATCGGTCAAGGTGGTTCTGGAGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATCCTGCAATACAGCAACTATTCCGTTATCTCGCCGGa          >  2:832678/1‑150 (MQ=255)
                                                                                                                                             cACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACCGCACCTATTCCGTTATCTCGCCGGAAg        >  1:1001353/1‑150 (MQ=255)
                                                                                                                                                   aCCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTTGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTtgtg  <  2:1196014/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTG  >  NC_000913/209024‑209320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑