Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,196,803 A→C 7.5% intergenic (+430/+64) icd → / ← ymfD isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase/e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,8030AC 7.5% 316.7 / 8.0 123intergenic (+430/+64)icd/ymfDisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase/e14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (67/45);  new base C (8/3);  total (75/48)
Fisher's exact test for biased strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

AATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGA  >  NC_000913/1196655‑1196950
                                                                                                                                                    |                                                                                                                                                   
aaTTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAg                                                                                                                                                    >  1:841365/1‑150 (MQ=255)
     ccACTCCTTACACCCGCTCAAATTTTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCgg                                                                                                                                               >  2:1632683/1‑150 (MQ=255)
     ccACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCCATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCgg                                                                                                                                               >  2:975875/1‑150 (MQ=255)
     ccACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAACGGCCGCGAGCGCgg                                                                                                                                               >  2:1212915/1‑150 (MQ=255)
     ccACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATCTAAAAAAGGCCGCGAGCGCgg                                                                                                                                               >  2:969617/1‑150 (MQ=255)
       aCTCCTTACACCCGCTCAAATTTTGTTAAATTGCCGGTTTTGTATCAACTAGTCACCCGGGCGTCGCCAGGGGCCAGCCAACAGGCATTGGGTGCAATACCCTTAGCGTTCAGGTACATGCGGAATGTAAAAACGCCCGCGAGCGCGGcc                                                                                                                                             >  2:709855/1‑150 (MQ=255)
       aCTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTAGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGcc                                                                                                                                             >  1:385270/1‑150 (MQ=255)
       aCTCCTTACACCCGCTCAAATATTGATAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGcc                                                                                                                                             <  2:1187789/150‑1 (MQ=255)
            ttACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGccttttt                                                                                                                                        <  2:1343143/150‑6 (MQ=255)
            ttACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTc                                                                                                                                        <  2:1257589/150‑1 (MQ=255)
             tACTCCCGATCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGAAAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGcctttttt                                                                                                                                       <  2:522653/150‑7 (MQ=255)
             tACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTca                                                                                                                                       >  1:518752/1‑150 (MQ=255)
               cacCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCCCCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAATAACGGCCGCGAGCGCGGCCCCTTcaca                                                                                                                                     >  1:1502175/1‑150 (MQ=255)
               cacCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCCCCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCTCGAGCGCGGCCCCTTcacc                                                                                                                                     >  1:233268/1‑149 (MQ=255)
                   cGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCacataca                                                                                                                                 <  2:1691241/150‑1 (MQ=255)
                   cGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCacataca                                                                                                                                 >  1:1665895/1‑150 (MQ=255)
                     cTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATc                                                                                                                               >  1:924935/1‑150 (MQ=255)
                     cTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATc                                                                                                                               <  1:582864/150‑1 (MQ=255)
                        aaaTATTGTTAAATTGGCGGTGTTGTATCAACTACTCACCCGGGACGCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCttt                                                                                                                            <  2:1750261/150‑1 (MQ=255)
                         aaTCTTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTa                                                                                                                           <  2:1096436/150‑1 (MQ=255)
                         aaTATTGTTAAATTGCCGGTTTTGTATCATCTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTa                                                                                                                           >  1:749440/1‑150 (MQ=255)
                           tatTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGt                                                                                                                         <  2:726928/150‑1 (MQ=255)
                             ttGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTAc                                                                                                                       >  1:1669798/1‑150 (MQ=255)
                              tGTTAAATTGCCGGTTTTGTATCAACTACTCACACGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGAAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCGTTCACATACATCTTTAGTACt                                                                                                                      >  2:88413/1‑150 (MQ=255)
                                     ttGCCGGGGTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGGACATGCGGATTGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTg                                                                                                               <  2:1218264/150‑1 (MQ=255)
                                       gCCGGTTTTGTATCGACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGtt                                                                                                             <  1:1457349/150‑1 (MQ=255)
                                          ggTTTTGTATCAACTACTCACCCGGGCCTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGAGTGCAGGGACATGCGGAATGTAAAAAAGGCCGCGATCGCGGCCCCTTCACATAGATCTTGAGTACTGAGACTGTTTaa                                                                                                          >  1:291035/1‑150 (MQ=255)
                                           gTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAAc                                                                                                         <  2:272891/150‑1 (MQ=255)
                                           gTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGACAACAGGCATTGGGTGCAATCACCTTAGCGATCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTATAGTACTGAGACTGTTTAAc                                                                                                         >  2:829638/1‑150 (MQ=255)
                                             tttGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCGCGCGACCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCt                                                                                                       >  1:1338128/1‑150 (MQ=255)
                                             tttGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCt                                                                                                       <  2:728641/150‑1 (MQ=255)
                                                 tATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAggg                                                                                                   <  2:1759418/150‑1 (MQ=255)
                                                        tactCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAattatt                                                                                            >  1:1283271/1‑150 (MQ=255)
                                                           tCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTGCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGAGTGTTTAACCTAGGGAATtattata                                                                                         >  1:554603/1‑149 (MQ=255)
                                                            cACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTCGGGATTTATTATCg                                                                                        >  1:100282/1‑150 (MQ=255)
                                                            cACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCTCATACATCTTTAGTACTGAGACTGTTTACCCTAGGGAATTATTATCg                                                                                        >  2:318816/1‑150 (MQ=255)
                                                               ccGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGtat                                                                                     <  2:840809/150‑1 (MQ=255)
                                                                   gACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATtata                                                                                 <  2:753446/150‑1 (MQ=255)
                                                                      tCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATGTTg                                                                              <  2:1355689/150‑1 (MQ=255)
                                                                       cGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGc                                                                             >  2:19867/1‑150 (MQ=255)
                                                                        gCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCa                                                                            >  1:1036787/1‑150 (MQ=255)
                                                                        gCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCa                                                                            >  1:867827/1‑150 (MQ=255)
                                                                        gCCAAGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAACTGCCGCGCGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCa                                                                            >  2:733464/1‑150 (MQ=255)
                                                                          cAGGGGACACCCAACAGGCAATGGGTGCAATCACCTTAGCGGGTAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCAtt                                                                          <  2:331303/150‑1 (MQ=255)
                                                                                   gCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAACGGCCGCGAGCGCGGCCCCGTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTTCATTACAACTccc                                                                 >  1:699479/1‑150 (MQ=255)
                                                                                    ccAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCa                                                                >  2:1424643/1‑150 (MQ=255)
                                                                                    ccAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCa                                                                <  1:1424883/150‑1 (MQ=255)
                                                                                     cAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCaa                                                               <  1:955642/150‑1 (MQ=255)
                                                                                       aCAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACa                                                             >  2:1623348/1‑150 (MQ=255)
                                                                                        cAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAg                                                            >  1:901736/1‑150 (MQ=255)
                                                                                          ggCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTg                                                          >  1:1596762/1‑150 (MQ=255)
                                                                                             aTTGTGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACa                                                       <  1:296791/150‑1 (MQ=255)
                                                                                             aTTGGGTGCAATCACCTTAGCGTTCAGTTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCATTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACa                                                       <  1:1212915/150‑1 (MQ=255)
                                                                                                  gTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGa                                                  >  1:690810/1‑150 (MQ=255)
                                                                                                    gCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAAc                                                <  1:1632683/150‑1 (MQ=255)
                                                                                                     cAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACt                                               >  1:883969/1‑150 (MQ=255)
                                                                                                      aaTCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCAAACAGTGACATATGAACtt                                              >  1:249566/1‑150 (MQ=255)
                                                                                                       aTCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTc                                             >  1:1696187/1‑150 (MQ=255)
                                                                                                        tCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTcc                                            <  1:893304/150‑1 (MQ=255)
                                                                                                         cACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCt                                           <  2:202474/150‑1 (MQ=255)
                                                                                                           ccTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGc                                         <  2:1165785/150‑1 (MQ=255)
                                                                                                           ccTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGc                                         <  1:1378001/150‑1 (MQ=255)
                                                                                                           ccTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGc                                         <  1:1385693/150‑1 (MQ=255)
                                                                                                            cTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCt                                        >  1:372097/1‑150 (MQ=255)
                                                                                                            cTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCTCATACATCTTTAGTTCTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCt                                        <  1:1167075/150‑1 (MQ=255)
                                                                                                            cTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCt                                        <  2:1525060/150‑1 (MQ=255)
                                                                                                                 cGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACt                                   >  2:1015674/1‑150 (MQ=255)
                                                                                                                   ttCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGTAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTcc                                 <  1:1132656/150‑1 (MQ=255)
                                                                                                                   ttCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTcc                                 >  1:1562452/1‑150 (MQ=255)
                                                                                                                   ttCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTcc                                 >  1:419717/1‑150 (MQ=255)
                                                                                                                     cAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCAc                               <  1:1189614/150‑1 (MQ=255)
                                                                                                                     cAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCAc                               <  2:924935/150‑1 (MQ=255)
                                                                                                                      aGGTACATGCGGAATGTAAAACAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACACCTCCCAACAGTGACATATGAACTTCCTGCTTGACTCCACg                              >  1:1125102/1‑150 (MQ=255)
                                                                                                                           cATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACttt                         >  1:1583884/1‑150 (MQ=255)
                                                                                                                           cATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACttt                         >  1:1687678/1‑150 (MQ=255)
                                                                                                                           cATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGGTTAACCTAGGGAATTATTATCGTATTATATTGCATTCCACCTCACTACAGTGACATATGAACTTCCTGCTTTACTCCACGACtgt                         >  1:875715/1‑148 (MQ=255)
                                                                                                                           cATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACACCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACttt                         <  2:1073693/150‑1 (MQ=255)
                                                                                                                              gCGGAATGTAAAACAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTACCCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGCCATATGAACTTCCTGCTTTACTCCACGACTGTAat                      >  1:108568/1‑150 (MQ=255)
                                                                                                                              gCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCCCATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAat                      >  2:1357120/1‑150 (MQ=255)
                                                                                                                              gCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAat                      >  2:38906/1‑150 (MQ=255)
                                                                                                                                ggAATGTAAAACAGGCCGCGCGCGCGGCCCCGTCACAGACCTGGTTAGTACTGAGACTGTTTAACCTAGGGAATAATTATCGTATTAAATTGAATTACAACACCCAAGAGTGCGATATGAACTTCCTGCTTTAATCCACGACTTTAatat                    >  2:300446/1‑150 (MQ=255)
                                                                                                                                     gTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCaa               >  2:806213/1‑150 (MQ=255)
                                                                                                                                      tAAAAACGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGACCTTCCTGCTTTACTCCACGACTTTAATATTTCAAg              >  2:1096062/1‑150 (MQ=255)
                                                                                                                                      tAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAg              >  1:73003/1‑150 (MQ=255)
                                                                                                                                      tAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAg              >  2:1244578/1‑150 (MQ=255)
                                                                                                                                       aaaaaaGGTCGCGAGCGCGGCCCCTTCTCATACATATTTAGTACTGAGTCAGTTATACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCCCGACTTTAATATTTCAAgg             <  2:291035/150‑1 (MQ=255)
                                                                                                                                       aaaaaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAgg             >  2:594307/1‑150 (MQ=255)
                                                                                                                                         aaggggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTATCCTAGGGAATTTTTATCGTTTTATATTGCATTACAACTCCCAACAGTGACATTTGAACTTCCTGCTTTAATACACTAATTTATTATTTCAAgtca           >  1:1051527/5‑147 (MQ=255)
                                                                                                                                          aggggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGCCTTTAATATTTCAAGGCCa          >  1:579694/4‑150 (MQ=255)
                                                                                                                                          aggggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCa          >  1:1223091/4‑150 (MQ=255)
                                                                                                                                           aaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCAt         <  2:385270/150‑1 (MQ=255)
                                                                                                                                           aaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCAt         <  1:1283933/150‑1 (MQ=255)
                                                                                                                                             ggCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAAATCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCAtgt       >  2:1157990/1‑150 (MQ=255)
                                                                                                                                              gCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTTTCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCAtgtg      <  1:969617/150‑1 (MQ=255)
                                                                                                                                              gCCGCGAGCGCGGCCCCTTAACATACATCGTTAGTACTGAGCCTGTTTAACCTAGGGAATTATTATCGGATTATATTGCATTACAAATACCAACAGTGACATATGAGCTTCCTGCTTTACTCCACGACTTTAATATTGCAAGGCCAtgtg      >  1:264539/1‑150 (MQ=255)
                                                                                                                                               ccGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGAGTGTTTAACCTAGGGAATTATTATCGTTTTATATTGCATTACATCTCCCAACAGTGACATATTAACTTCCTGCTTTACTCCACGACTTTAATATTTCAATGCCATGTga     >  1:1007799/1‑150 (MQ=255)
                                                                                                                                               ccGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTATTATTTCAAGGCCATGTga     >  2:1151576/1‑150 (MQ=255)
                                                                                                                                                cgcgAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCTTTACAACTCCCAACAGTGACATTTGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTgaa    >  1:211717/1‑150 (MQ=255)
                                                                                                                                                cgcgAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTgaa    >  2:1232427/1‑150 (MQ=255)
                                                                                                                                                  cgAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTgaaga  <  2:233268/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGA  >  NC_000913/1196655‑1196950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: