Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 209,172 C→G 5.8% N184K (AAC→AAG) ‡ accA → acetyl‑CoA carboxylase, carboxytransferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913209,1720CG 5.8% 409.3 / 10.8 131N184K (AAC→AAG) ‡accAacetyl‑CoA carboxylase, carboxytransferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (67/56);  new base G (4/4);  total (71/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 134 total aligned reads displayed.

CAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTGC  >  NC_000913/209024‑209321
                                                                                                                                                    |                                                                                                                                                     
cAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCTGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGACATTGCACGCAAcc                                                                                                                                                      >  2:286377/1‑150 (MQ=255)
  gcgcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTg                                                                                                                                                    >  1:965456/1‑150 (MQ=255)
  gcgcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTATAAGATGCCTATCATCCCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTg                                                                                                                                                    >  2:1088898/1‑150 (MQ=255)
   cgcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGc                                                                                                                                                   <  2:830375/150‑1 (MQ=255)
    gcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCCCCTTTATCGACACCCCGGGGGCATATCCTGGAGTTGGCGCAGAAGCGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCg                                                                                                                                                  >  2:1007206/1‑150 (MQ=255)
    gcCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCg                                                                                                                                                  >  1:1277391/1‑150 (MQ=255)
        gAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCCGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGaa                                                                                                                                              <  2:1465973/150‑1 (MQ=255)
           ggTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAGGTTGCGTGCAATg                                                                                                                                           <  1:818674/150‑1 (MQ=255)
            gTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTTCACGCAGGTTGCGTGCAATgg                                                                                                                                          <  1:1254893/150‑2 (MQ=255)
            gTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTTCACGCAGGTTGCGTGCAATgg                                                                                                                                          <  1:631809/150‑2 (MQ=255)
             ttACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGtc                                                                                                                                         >  1:1345569/1‑150 (MQ=255)
               aCCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACCCCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGtctc                                                                                                                                       >  1:1572748/1‑150 (MQ=255)
                ccGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCg                                                                                                                                      >  1:805266/1‑150 (MQ=255)
                ccGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCg                                                                                                                                      >  2:664128/1‑150 (MQ=255)
                 cGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTAACGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGc                                                                                                                                     >  2:826921/1‑150 (MQ=255)
                    aaaGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTc                                                                                                                                  <  2:677056/150‑1 (MQ=255)
                            gCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTAcc                                                                                                                          >  1:1278710/1‑150 (MQ=255)
                                cTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTa                                                                                                                      >  2:1479993/1‑150 (MQ=255)
                                cTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCCACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTa                                                                                                                      >  2:955803/1‑150 (MQ=255)
                                   aTGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGtt                                                                                                                   <  1:310658/150‑1 (MQ=255)
                                    tGCTAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGttt                                                                                                                  >  1:942114/1‑150 (MQ=255)
                                    tGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGttt                                                                                                                  >  2:1665533/1‑150 (MQ=255)
                                     gCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTg                                                                                                                 <  2:1345569/150‑1 (MQ=255)
                                      cAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGt                                                                                                                >  1:1608300/1‑150 (MQ=255)
                                      cAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGt                                                                                                                >  1:1019936/1‑150 (MQ=255)
                                      cAAATGGCTGAACGCTTTAAGATGCCAATCATCACATTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGAGGTCTGTCTGAAGCCATTGCACGCAACCTGCGAGAAATGTCTCGCCTCGGCGTACCGGGAGTTAGt                                                                                                                >  1:871580/1‑150 (MQ=255)
                                          tGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACgg                                                                                                            >  1:525834/1‑150 (MQ=255)
                                           ggCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCACTGGGGATTATCCTGGCGTGGGAGAAGAAGAGCTTGGTCAGTCTGAAGACATTGCACGCAACCTGCGTGAAATGTCTAGCCTCGGCGTACCGGTAGTTTGTACGGt                                                                                                           >  1:1280066/1‑150 (MQ=255)
                                               gAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCC‑GGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCg                                                                                                      >  1:1773431/1‑150 (MQ=255)
                                                aaCGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGGGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCg                                                                                                      <  1:72748/150‑1 (MQ=255)
                                                aaCGCTTTAAGATGCCTATCATCACCTTTATCGACAACCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCTTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCg                                                                                                      <  2:1734234/150‑1 (MQ=255)
                                                      ttAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAg                                                                                                <  2:1800742/150‑1 (MQ=255)
                                                       tAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAgg                                                                                               <  1:1665533/150‑1 (MQ=255)
                                                             gCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTc                                                                                         >  2:1362992/1‑150 (MQ=255)
                                                              ccTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTATGTACGGTTAGCGGTGAAGGTGGTTCt                                                                                        >  2:1344860/1‑150 (MQ=255)
                                                                 atcatcACCTTTATCGCGACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGc                                                                                     <  1:145918/150‑1 (MQ=255)
                                                                  tcatcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGTGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCg                                                                                    <  1:1592955/150‑1 (MQ=255)
                                                                  tcatcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCg                                                                                    <  1:251212/150‑1 (MQ=255)
                                                                  tcatcaCCTTTAGCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTGCTGGCg                                                                                    <  2:1394576/150‑1 (MQ=255)
                                                                    atcaCCTATATCGACAGCCCCGGGGCTT‑TCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCTACCTGCGTGATATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTg                                                                                 <  2:685008/150‑1 (MQ=255)
                                                                     tcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTg                                                                                 <  1:664128/150‑1 (MQ=255)
                                                                     tcaCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTg                                                                                 >  1:1039723/1‑150 (MQ=255)
                                                                      caCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTTTCGGTGAAGGTGGTTCTGGCGGTgc                                                                                >  1:224819/1‑150 (MQ=255)
                                                                       aCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGGCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcg                                                                               <  2:1447773/150‑1 (MQ=255)
                                                                       aCCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGGGGGCAGTCTGAAGCCATTGCACGCACCCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcg                                                                               <  2:1198962/150‑1 (MQ=255)
                                                                        ccTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTgcgc                                                                              >  2:855102/1‑150 (MQ=255)
                                                                        ccTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGCCGTACCGGTAGTTTGTACGGTTATCGGTGATGGTGGTTCTGGCGGTgcgc                                                                              >  2:659904/1‑150 (MQ=255)
                                                                          tttATCGTCACCCCGGGGGCTTATGCTGGCGGGGGCGCAGAAGAGCGGGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGTCGGTGCGCTg                                                                            <  1:505655/150‑1 (MQ=255)
                                                                          tttATCGACACCCCGGGGGCTTATCCTGGCCGGGGCGCAGAAGAGCGTTGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGGGAAGGTGGTTCTGGCGGTGCGCTg                                                                            <  2:142027/150‑1 (MQ=255)
                                                                           ttATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGGACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTgg                                                                           <  2:525834/150‑1 (MQ=255)
                                                                           ttATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTgg                                                                           >  1:221274/1‑150 (MQ=255)
                                                                            aaTCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGGCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGc                                                                          <  1:1502563/149‑1 (MQ=255)
                                                                               cGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGTAGGTGGTTCTGGCGGTGCGCTGGCGAt                                                                       >  1:127396/1‑150 (MQ=255)
                                                                               cGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGAAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAt                                                                       >  2:1682894/1‑150 (MQ=255)
                                                                                gACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAtt                                                                      >  1:885115/1‑150 (MQ=255)
                                                                                  cacCCCGGGTGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                                                    <  2:849020/150‑1 (MQ=255)
                                                                                  cacCCCGGGGGCGTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTgg                                                                    <  1:1102531/150‑1 (MQ=255)
                                                                                   cccccGGGGGCTTATCCTGGCGGGGGCGCAGAAGAGCGGGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGc                                                                   <  1:321424/149‑1 (MQ=255)
                                                                                    ccccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCg                                                                  <  2:1621444/150‑1 (MQ=255)
                                                                                    ccccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCg                                                                  <  2:798448/150‑1 (MQ=255)
                                                                                      ccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGGCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGGCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTg                                                                <  1:1171809/150‑1 (MQ=255)
                                                                                          gggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTTAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCg                                                            <  2:955940/150‑1 (MQ=255)
                                                                                          gggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCg                                                            >  2:372884/1‑150 (MQ=255)
                                                                                          gggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGGCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCg                                                            <  1:1780161/150‑1 (MQ=255)
                                                                                           ggCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGa                                                           >  1:1001762/1‑150 (MQ=255)
                                                                                              ttATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATaa                                                        >  2:159343/1‑150 (MQ=255)
                                                                                               tATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATaaa                                                       >  2:585465/1‑150 (MQ=255)
                                                                                                aTCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAg                                                      >  1:777956/1‑150 (MQ=255)
                                                                                                 tCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGt                                                     <  2:1454476/150‑1 (MQ=255)
                                                                                                 tCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGt                                                     <  1:826921/150‑1 (MQ=255)
                                                                                                  ccTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTg                                                    >  2:1720216/1‑150 (MQ=255)
                                                                                                   cTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGa                                                   >  2:1015095/1‑150 (MQ=255)
                                                                                                          gggCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgct                                            <  1:1448549/150‑1 (MQ=255)
                                                                                                            gcgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCGACCTGCGTGAATTGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGGGATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGGGATTGGCGTGGGCGAGAAAGTGAATAtgctgc                                          >  1:76220/1‑150 (MQ=255)
                                                                                                             cgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCa                                         >  2:1201484/1‑150 (MQ=255)
                                                                                                             cgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGAGAAAGTGAATATGCTGCa                                         >  2:1801940/1‑150 (MQ=255)
                                                                                                              gcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCaa                                        <  1:733731/150‑1 (MQ=255)
                                                                                                              gcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCaa                                        >  2:424580/1‑150 (MQ=255)
                                                                                                               cAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAATTGAATATGCTGCAAt                                       >  2:1814589/1‑150 (MQ=255)
                                                                                                                 gaagaGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATAc                                     >  1:303261/1‑150 (MQ=255)
                                                                                                                  aagaGCTTGCTCAGTCTGAAGCCATTGCATTCAAGATGCGTGAAATGTCTCTCATAGTCGTACCGGTAGTTTGTAAGGTTATCGGTGAAGGTTTTTCTGTCGGTGCTCTTGCGATTGGCGTGGGCGATAAAGTTAATATGCTGCAATACa                                    <  1:286377/150‑1 (MQ=255)
                                                                                                                   agagCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAg                                   <  2:1759218/150‑1 (MQ=255)
                                                                                                                   agagCGGGGGCAGGCGGTAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGGTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAg                                   <  1:1119017/150‑1 (MQ=255)
                                                                                                                      gCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCAc                                >  1:231610/1‑150 (MQ=255)
                                                                                                                        gTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCt                              >  1:1049009/1‑150 (MQ=255)
                                                                                                                          ggTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGAGAAAGTGAATATGCTGCAATACAGCAACTAt                            >  1:1473275/1‑150 (MQ=255)
                                                                                                                            tCAGTCTGAAGCCATTGCTCTCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGGTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTc                          <  1:442735/150‑1 (MQ=255)
                                                                                                                             gaGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCTGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCTCCTATTcc                         <  2:883013/149‑1 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGt                       >  2:652606/1‑150 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGt                       <  1:1478684/150‑1 (MQ=255)
                                                                                                                                 cTGAAGCCAGTGCACGCAACCTGCGGGAAATGTCCCGACTCCGCGTACCGGTAGTTTTTTCTGTTATCTGTGAAGGTGGTTCTGGCGTTGCGCTGGCGATTGGCGTGGGCGATTAAGTGAATATGCTGGAATACAGCACCTATTCCGTTa                     <  2:273728/150‑1 (MQ=255)
                                                                                                                                  tGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAt                    >  1:1213308/1‑150 (MQ=255)
                                                                                                                                  gcgagacATTTCACGCAGGTTGCGTGCAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTTGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAt                    >  2:68024/7‑150 (MQ=255)
                                                                                                                                   gAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAtc                   >  1:393501/1‑150 (MQ=255)
                                                                                                                                     aGCCATTGGACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGTAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAtctc                 <  1:11960/150‑1 (MQ=255)
                                                                                                                                     aGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTCTTCCGTTAtctc                 >  1:1752566/1‑150 (MQ=255)
                                                                                                                                        cATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGcc              <  2:1288027/150‑1 (MQ=255)
                                                                                                                                             cACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACCGCACCTATTCCGTTATCTCGCCGGAAg         >  1:1001353/1‑150 (MQ=255)
                                                                                                                                                   aCCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTTGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTtgtg   <  2:1196014/150‑1 (MQ=255)
                                                                                                                                                   aCCTGCGGGAAATGTCTCGCCGCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCATCTATTCCGTTATCTCGCCGGAAGGTtgtg   <  2:1533393/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTGC  >  NC_000913/209024‑209321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑