Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,200,914 T→A 6.5% K40I (AAA→ATA) ‡ xisE ← e14 prophage; putative excisionase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,200,9140TA 6.5% 407.1 / 12.2 148K40I (AAA→ATA) ‡xisEe14 prophage; putative excisionase
Reads supporting (aligned to +/- strand):  ref base T (68/68);  new base A (6/4);  total (76/72)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 151 total aligned reads displayed.

TTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCT  >  NC_000913/1200766‑1201063
                                                                                                                                                    |                                                                                                                                                     
ttAGTGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGgtt                                                                                                                                                      <  1:90739/150‑1 (MQ=255)
 tAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTATTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGttta                                                                                                                                                     >  1:148148/1‑149 (MQ=255)
   gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCAt                                                                                                                                                   <  1:1673004/150‑1 (MQ=255)
    ggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATg                                                                                                                                                  <  1:1044597/150‑1 (MQ=255)
     gACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATgg                                                                                                                                                 <  1:627473/150‑1 (MQ=255)
       cTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCCCGCACTCGCCATAACCGGCCTTGTTTCATGGcc                                                                                                                                               >  2:460393/1‑150 (MQ=255)
          aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGt                                                                                                                                            <  2:323632/150‑1 (MQ=255)
          aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCATGAAACAAGGCCGGt                                                                                                                                            <  2:1248138/150‑1 (MQ=255)
            aCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                                                                          <  2:97958/150‑1 (MQ=255)
             cATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATCGCCGGTggc                                                                                                                                         >  1:296975/1‑149 (MQ=255)
               ttattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACa                                                                                                                                       >  2:230507/1‑150 (MQ=255)
                 attCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                                                                     >  1:57567/1‑150 (MQ=255)
                   tCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTATCTTGATTACAGGTGTGACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                                                                   <  1:1341723/150‑1 (MQ=255)
                   tCTTACGCGGTCTGGCGGCCATTACTCAACATCCGGTGCAAAAGTAATGAGTCAGTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCTCGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                                                                   <  1:1349230/150‑1 (MQ=255)
                    cTTACTCGTTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaata                                                                                                                                  <  2:238718/150‑1 (MQ=255)
                     ttACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaataa                                                                                                                                 >  2:1022022/1‑150 (MQ=255)
                        cgcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                                                                              <  1:141546/150‑1 (MQ=255)
                         gcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTc                                                                                                                             <  1:543351/150‑1 (MQ=255)
                         gcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTc                                                                                                                             >  2:203244/1‑150 (MQ=255)
                             tCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCCCGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                                                                         >  2:945801/1‑150 (MQ=255)
                                ggCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                                                                      <  2:1369811/150‑1 (MQ=255)
                                ggCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                                                                      >  1:1514196/1‑150 (MQ=255)
                                 gCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGc                                                                                                                     <  2:608542/150‑1 (MQ=255)
                                  cTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCa                                                                                                                    <  1:1040431/150‑1 (MQ=255)
                                     ccATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATaa                                                                                                                 >  2:249019/1‑150 (MQ=255)
                                      cATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAac                                                                                                                >  2:1078645/1‑150 (MQ=255)
                                         tACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                                                                             <  1:1177660/150‑1 (MQ=255)
                                         tACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                                                                             >  1:519567/1‑150 (MQ=255)
                                          aCTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGCACAAAATAAATTCTGCTTATCATAacgacg                                                                                                            >  2:311952/1‑150 (MQ=255)
                                           cTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGc                                                                                                           >  1:856637/1‑150 (MQ=255)
                                             cAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCTTAACGACGCaa                                                                                                         >  2:1306572/1‑150 (MQ=255)
                                             cAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACTACGCaa                                                                                                         >  1:1097624/1‑150 (MQ=255)
                                             cAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCaa                                                                                                         >  2:718750/1‑150 (MQ=255)
                                             cAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCaa                                                                                                         >  2:1152140/1‑150 (MQ=255)
                                              aaaaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAt                                                                                                        >  1:1125734/1‑150 (MQ=255)
                                               aaaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAtg                                                                                                       >  2:949700/1‑150 (MQ=255)
                                                 aTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCCGCCTTGTTTCATGGCCGGAGGACAAAATAAAATCTGCTTAGCATAACGACGCAAtgtg                                                                                                     >  2:1344928/1‑150 (MQ=255)
                                                  tCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTgg                                                                                                    <  2:184538/150‑1 (MQ=255)
                                                  tCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTgg                                                                                                    <  1:1390927/150‑1 (MQ=255)
                                                  tCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTgg                                                                                                    <  2:675350/150‑1 (MQ=255)
                                                       tGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGacaca                                                                                               <  2:1780679/150‑1 (MQ=255)
                                                        gCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCAGAACGCCGCAATGTGGacaccc                                                                                              >  1:931883/1‑148 (MQ=255)
                                                         cAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACt                                                                                             >  1:1692320/1‑150 (MQ=255)
                                                         cAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGAAGCAATGTGGACACACt                                                                                             >  1:1731229/1‑150 (MQ=255)
                                                          aaaaGTAATGAGTCATTTTTCTTGATTATAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGGCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGCCGCAATGTGGACACACtt                                                                                            >  1:64631/1‑150 (MQ=255)
                                                          aaaaGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACtt                                                                                            <  1:810300/150‑1 (MQ=255)
                                                            aaGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgg                                                                                          <  1:392746/150‑1 (MQ=255)
                                                                  tGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGAtt                                                                                    <  2:662975/150‑1 (MQ=255)
                                                                         tttttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTg                                                                             <  2:383606/150‑1 (MQ=255)
                                                                         tttttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTAGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTg                                                                             <  1:1331881/150‑1 (MQ=255)
                                                                           tttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTa                                                                           <  1:1745799/150‑1 (MQ=255)
                                                                                 gATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTc                                                                     <  2:1750467/150‑1 (MQ=255)
                                                                                  aTTACAGGTGTTACCAACTCCCCAACTACCTCGGCGTCCTCACGCACTCGCCATAACCGGCATTGGTTCATGGCAGTTGGCGAAAATAAATTCTGCTTAGAAGACCTCCGCATTGTGGCCCCAGGTGGAGGATGACTTCTGTATTTTTcc                                                                    >  1:299480/1‑149 (MQ=255)
                                                                                   ttACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcag                                                                   <  1:412768/150‑1 (MQ=255)
                                                                                    tACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                                                                  <  2:1322671/150‑1 (MQ=255)
                                                                                    tACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                                                                  <  2:1392131/150‑1 (MQ=255)
                                                                                    tACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                                                                  >  1:447690/1‑150 (MQ=255)
                                                                                    tACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTGTCATGGCCGGTGGACATAATAAATGCTGCTTAGCATAACGCCGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                                                                  >  1:328378/1‑150 (MQ=255)
                                                                                      cAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTATCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGAAGCACTGGGAGGATTACTTCTCTATTTTTcagcat                                                                >  1:316600/1‑149 (MQ=255)
                                                                                              aCCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttct                                                        >  2:554928/1‑150 (MQ=255)
                                                                                               ccAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttctt                                                       >  1:1259933/1‑150 (MQ=255)
                                                                                                cAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttcttc                                                      >  2:1527675/1‑150 (MQ=255)
                                                                                                  aCTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCaa                                                    <  1:982894/150‑1 (MQ=255)
                                                                                                   cTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAag                                                   <  2:803109/150‑1 (MQ=255)
                                                                                                     ccccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCATGTGTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTTTTCAagag                                                 >  1:1277766/1‑150 (MQ=255)
                                                                                                       ccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTCGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGTGtt                                               >  1:159872/1‑150 (MQ=255)
                                                                                                        cAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTa                                              >  1:979175/1‑150 (MQ=255)
                                                                                                        cAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTa                                              >  2:751138/1‑150 (MQ=255)
                                                                                                         aactaactCGGCGTCCTCACGCTCTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTc                                                                     <  2:766673/126‑1 (MQ=255)
                                                                                                         aactaactCGGCGTCCTCACGCGCTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTc                                                                     >  1:766673/1‑126 (MQ=255)
                                                                                                          actaactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAAc                                            <  1:949700/150‑1 (MQ=255)
                                                                                                             aactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATATCGACGCAATGTGGCGACACTTGGAGGAGTACTTCTGTATTTTTCAGCAGCCCGTTCTTCAAGAGTTAACAtt                                         >  2:901004/1‑150 (MQ=255)
                                                                                                             aactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACAtt                                         >  2:197092/1‑150 (MQ=255)
                                                                                                               ctCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTg                                       <  1:1176142/150‑1 (MQ=255)
                                                                                                               ctCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTg                                       <  1:1782932/150‑1 (MQ=255)
                                                                                                                tCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGa                                      >  2:332963/1‑150 (MQ=255)
                                                                                                                  ggCGTCCTCACGCACTCGACATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAg                                    <  2:1185337/150‑1 (MQ=255)
                                                                                                                   gCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACCCTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGc                                   >  2:1445725/1‑150 (MQ=255)
                                                                                                                   gCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGc                                   >  2:802976/1‑150 (MQ=255)
                                                                                                                        cTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATg                              >  2:1069453/1‑150 (MQ=255)
                                                                                                                             ggtcTCGCCAAAACCGGCCTTGTTTTATGGCCGGTGTACAAAATAAATTCTGTTTAGCATAACGACGCAATGTGGACACACTTGGTTGAATACTTCTTTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATc                         <  2:182770/147‑1 (MQ=255)
                                                                                                                                cTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGCCGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCCGCCCATTCTTCAAGAGTGAACATTTGAAGCAGATGCGAGCAcc                      >  2:304108/1‑150 (MQ=255)
                                                                                                                                cTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCAcc                      <  2:1467457/150‑1 (MQ=255)
                                                                                                                                cTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCAcc                      >  1:1549898/1‑150 (MQ=255)
                                                                                                                                cTCGCCATAACATGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCAcc                      <  1:1101943/150‑1 (MQ=255)
                                                                                                                                 tCGCCATAACCGGACTTGTTTCATGGCCGGTGGACAAAAAAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCt                     <  1:311952/150‑1 (MQ=255)
                                                                                                                                    ccATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGATTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttat                  <  2:708981/150‑1 (MQ=255)
                                                                                                                                     gtccaCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatt                 >  2:1680933/5‑150 (MQ=255)
                                                                                                                                     cATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatt                 <  1:736335/150‑1 (MQ=255)
                                                                                                                                      tccaCCGGCCATGAAACAAGGCCGGTGGCCAAAATAAATTCTGCTTAGCATAACGACGCAATGTGAACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCCTATGCGCTCACCttatta                >  1:164673/4‑150 (MQ=255)
                                                                                                                                        caCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtact              >  2:495888/2‑150 (MQ=255)
                                                                                                                                        caCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGAGTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAAGATTTGAAGCATATGCGATCACCTTATtact              >  2:302141/2‑150 (MQ=255)
                                                                                                                                         aCCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTATCATAACGAAGCAATGTGGACACACTTGGAGGATTACTTCTGTATATTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtacta             <  2:1561022/150‑1 (MQ=255)
                                                                                                                                          ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATTACCTTATtactac            >  2:8913/1‑150 (MQ=255)
                                                                                                                                          ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtactac            <  2:57567/150‑1 (MQ=255)
                                                                                                                                           cGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTaca           >  1:1446627/1‑150 (MQ=255)
                                                                                                                                            ggCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGAGATCACCTTATTACTacaa          >  1:1146676/1‑149 (MQ=255)
                                                                                                                                               cTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCATAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTaa       <  1:203244/150‑1 (MQ=255)
                                                                                                                                                  gTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTg    >  2:1619181/1‑150 (MQ=255)
                                                                                                                                                    ttCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCt  <  1:1692615/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGCT  >  NC_000913/1200766‑1201063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: